| General information | |||
| TC004501 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 3684548..3691317 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004501 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10670 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) -
Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna bent - Penetrance (percentage of animals/eggs on slide): less than 30%
labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs segment potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna bent - Penetrance (percentage of animals/eggs on slide): less than 30%
labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%
legs segment potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004501 RA
MLREHSDID RHSHWADVK RKVDSDARY KAVDSSGQR EDWFREYCK ILKEEKKKA KEKDREHKR DKEKHKKKD KDKDKERHS KDEKEKDKD KEKDKSKKE EDGDVSNKE VEETEPEET DDEKAQEQR DKDRQARAE ASLREREKE VQRTLATHM RDRDKEREQ HKRDEAIQH FNALLADLV RNPELSWRE VKRILRKDH RWDLADSLS REDKEKLFN EHIEHLLRK KREKFRELL DETPDVTLT SSWKEIKKI IKEDPRYTK FASSERCER EFKDYLKDK LITAKGQFK ELLQETKLI THKSLSNLR ENQGFMQEI EDILKNDKR YLVLDHIPQ ERTQLILNY LEELDRRGP PPPPTASEP NRRSIK
- >TC004501 RB
MEETDVSME YDGADVDGV AQEEYEEDG GFENDIELG DDFQMRGRG PSGFRGRGR AWPGPPGPP GPRGEGPRF RGRGFGPGG PPPFRGRGG PPPPMFGRG GPPRNPPPG QGFNGPPNF NGPNWGGPM GPPGTMGGP PNMMGPNGP PFGPPGMMG APPGGPMGA QGGTTNGQF PPVGQNPTT GLDANSEIW VETKTSEGK SYFYNARTR ETTWTKPEG PNVKVISQD QVEAMAQAA TTGLAQGTS TAAQAALAQ ANVSNKPEV ESEESKGDA KQAGNAMPQ NMLAGPPPG MPPFGGPPG QFGAPPFGL PPPGFQGTW PAPGQAAWA GGPPGAAPW GMPPGLLTG LPGAIPAID EAAVLAKVD PEIIAKASE WSEHKAPDG RFYYYNAKK GESVWEKPQ ALKDLETAK LAAAQGIST RPGTEVIAA VETGKPNAA VVQVANGDA VKDIVIKEE DDKIKKPQD ETKKKKEEE EKKETKSQD KSRPVSSTP VPGTPWCVV WTGDGRVFF YNPSSRTSV WERPDELIK RTDVDKMVA TPPDSVGSQ TKPDAESPS KKRVSDDSD SEEETPAKK PKKEEITST NGTTPQSAR KIDIGKEAA IEAEVRAAK ERAIVPLET RIKSFKEML AEKNVSAFS TWEKELHKI VFDTRYLLL TSKERKQVF EKYVKERAE EERREKRNK LREKKDAFR KLLSESHLH GKSSFSDFA QKFAKDERF KGVEKMRER ESLFNEYLI EVRKREKEE KNQRREQVK KDFFAMLRE HSDIDRHSH WADVKRKVD SDARYKAVD SSGQREDWF REYCKILKE EKKKAKEKD REHKRDKEK HKKKDKDKD KERHSKDEK EKDKDKEKD KSKKEEDGD VSNKEVEET EPEETDDEK AQEQRDKDR QARAEASLR EREKEVQRT LATHMRDRD KEREQHKRD EAIQHFNAL LADLVRNPE LSWREVKRI LRKDHRWDL ADSLSREDK EKLFNEHIE HLLRKKREK FRELLDETP DVTLTSSWK EIKKIIKED PRYTKFASS ERCEREFKD YLKDKLITA KGQFKELLQ ETKLITHKS LSNLRENQG FMQEIEDIL KNDKRYLVL DHIPQERTQ LILNYLEEL DRRGPPPPP TASEPNRRS IK
- >TC004501 RC
MEETDVSME YDGADVDGV AQEEYEEDG GFENDIELG DDFQMRGRG PSGFRGRGR AWPGPPGPP GPRGEGPRF RGRGFGPGG PPPFRGRGG PPPPMFGRG GPPRNPPPG QGFNGPPNF NGPNWGGPM GPPGTMGGP PNMMGPNGP PFGPPGMMG APPGGPMGA QGGTTNGQF PPVGQNPTT GLDANSEIW VETKTSEGK SYFYNARTR ETTWTKPEG PNVKVISQD QVEAMAQAA TTGLAQGTS TAAQAALAQ ANVSNKPEV ESEESKGDA KQAGNAMPQ NMLAGPPPG MPPFGGPPG QFGAPPFGL PPPGFQGTW PAPGQAAWA GGPPGAAPW GMPPGLLTG LPGAIPAID EAAVLAKVD PEIIAKASE WSEHKAPDG RFYYYNAKK GESVWEKPQ ALKDLETAK LAAAQGIST RPGTEVIAA VETGKPNAA VVQVANGDA VKDIVIKEE DDKIKKPQD ETKKKKEEE EKKETKSQD KSRPVSSTP VPGTPWCVV WTGDGRVFF YNPSSRTSV WERPDELIK RTDVDKMVA TPPDSVGSQ TKPDAESPS KKRVSDDSD SEEETPAKK PKKEEITST NGTTPQSAR KIDIGKEAA IEAEVRAAK ERAIVPLET RIKSFKEML AEKNVSAFS TWEKELHKI VFDTRYLLL TSKERKQVF EKYVKERAE EERREKRNK LREKKDAFR KLLSESHLH GKSSFSDFA QKFAKDERF KGVEKMRER ESLFNEYLI EVRKREKEE KNQRREQVR MRCFLSQTY EKVTM
- >TC004501 RA
atgctacgc gaacactct gatatcgac cgtcattcc cactgggcc gatgtgaaa cgaaaagtt gattcggac gctcgatac aaagccgtt gattcgtcc ggacaacgt gaagattgg ttcagagaa tattgcaag atacttaaa gaagagaaa aagaaagcg aaagagaaa gatcgcgaa cacaaacgt gataaagaa aagcataag aagaaggat aaagataag gataaggaa cgtcattcg aaagacgaa aaagagaaa gataaagac aaggagaaa gataaatcg aagaaagaa gaagacgga gatgtgtcg aataaagaa gttgaagaa acggaaccg gaagaaacc gacgacgaa aaagcacaa gaacaaaga gataaagat aggcaagcc agggcggag gcgagtctg agagagagg gagaaggag gtacaaagg actctagcg acgcatatg agggaccga gataaggag agggagcaa cacaaacgg gacgaggct atacaacat tttaatgcg ttattggcc gatttggtg cgcaatccc gagctgagc tggcgcgag gttaagcgc attttgcga aaagaccac cgttgggac ttggctgat tcgctgtcg cgtgaagat aaggaaaaa ttattcaat gaacacatt gaacatttg ttgaggaag aagagagag aagttccgc gagttattg gatgaaacc cccgatgtt actttaacc agtagttgg aaagaaatt aaaaaaatt ataaaagag gatccccga tatacgaaa tttgcgtca agcgaacgg tgtgaacgt gaatttaag gattatctc aaagataaa ctaataacc gccaaagga caattcaag gaattgtta caggaaact aaacttatt acacataaa tcgttatct aatctcaga gagaatcaa ggatttatg caagaaatt gaggatatt ttaaagaat gataagaga tatcttgtc ttggatcat attccgcaa gagaggact cagttgatt ctgaattat ttggaagaa ctcgataga aggggacca cctccgcca cctacggct agtgaacct aataggaga tctattaaa tag
- >TC004501 RB
atggaagaa acagacgtc tccatggaa tacgacggc gccgacgtc gacggcgtc gcccaagag gaatacgaa gaggacgga ggcttcgaa aacgacatt gaactcgga gatgacttc caaatgcga ggccgaggt ccttcaggg ttcaggggc cgcggacgc gcgtggccg ggtcctcca ggtccgcca ggaccgcgt ggagaaggt ccccggttt cgaggaagg ggatttggg cccggtggc cctcctccg tttcgtgga cgtggagga ccacctcct ccgatgttt ggacgtggg ggacctccc agaaaccca ccaccaggg cagggattc aatggacct ccgaatttt aatgggcct aactgggga ggaccgatg gggcccccg gggacgatg ggtggaccg cccaatatg atgggaccc aatggaccg ccgttcgga ccaccgggc atgatgggg gcgcccccg ggtggaccc atgggcgct cagggcggg acgaccaat gggcagttt ccaccagta gggcaaaat cccacaaca gggttagat gcaaatagt gaaatttgg gtagagact aaaacctca gaaggcaag tcatatttt tataatgct cgtactaga gaaaccact tggactaag ccagaaggt cctaatgta aaagtaatc tctcaggat caagtggaa gcaatggca caagcggcc accacaggt ttagcccaa ggaacgtca actgcagct caggcggcg ttagcgcag gctaacgtt tcaaataaa cctgaagtt gagtcagag gaatcgaaa ggcgatgcc aaacaagct gggaacgca atgcctcaa aacatgtta gcgggtcct ccgccaggt atgccccca tttgggggt cccccaggt cagtttgga gcgcctcca ttcggctta ccaccgcca ggattccaa gggacatgg ccggctcca ggtcaagca gcatgggca ggggggcca ccaggggct gctccgtgg ggtatgccg ccaggtctt cttacaggt ttaccagga gctatccca gcgattgac gaagcagcg gttttggct aaagtcgat ccggaaatc atcgccaaa gcgtccgaa tggagcgaa cacaaagca cccgacgga cgtttctat tattacaac gccaagaaa ggggaatcg gtttgggaa aaaccgcaa gctcttaag gatttggaa actgcgaaa ctggctgca gctcaaggg atttcaaca agaccggga accgaagtt atagctgct gttgagaca ggtaagcca aacgctgca gttgtccaa gtggccaat ggggacgca gtgaaagac attgtcata aaggaggaa gatgacaag atcaaaaag ccccaagac gaaacgaaa aagaagaag gaagaagaa gaaaaaaag gaaactaaa agtcaagat aaatcgcgc ccagtgtca agtaccccc gtcccagga accccatgg tgtgttgtg tggaccggc gatggaaga gttttcttc tacaaccca tcgtcaagg acatccgtt tgggagcgt cctgatgag ctaataaaa cgaacagat gtggataaa atggtagcg acacctccc gattccgtc ggatctcaa accaaacca gacgctgaa agtccgtcg aaaaaacgc gtatccgac gacagcgac agtgaggaa gaaacccca gctaaaaaa ccgaaaaaa gaagaaata accagtacg aacggtacc actccacaa tcagctcgt aaaatcgat attggcaaa gaagcagcc attgaagcg gaagtacgt gcggccaaa gaacgtgct attgtccca ctggaaact cgaattaaa tcatttaaa gaaatgctg gccgagaaa aacgtttcc gcgtttagt acttgggaa aaagaacta cataaaatt gttttcgat acacggtat ctccttcta acgtccaag gaacgcaaa caagtgttt gaaaagtac gttaaagaa cgagccgag gaagagcga agagagaaa cggaataag ttgagagag aagaaggac gcgtttagg aaactgttg agtgagtcg catctacac ggcaaatcg tcgtttagt gacttcgca cagaagttc gcaaaggac gaacgattt aaaggggtt gagaaaatg cgcgaacgg gaaagtctc ttcaatgaa tatttaatc gaagtaaga aagcgcgaa aaagaagaa aagaatcag cgacgagaa caggtaaag aaagatttc tttgcaatg ctacgcgaa cactctgat atcgaccgt cattcccac tgggccgat gtgaaacga aaagttgat tcggacgct cgatacaaa gccgttgat tcgtccgga caacgtgaa gattggttc agagaatat tgcaagata cttaaagaa gagaaaaag aaagcgaaa gagaaagat cgcgaacac aaacgtgat aaagaaaag cataagaag aaggataaa gataaggat aaggaacgt cattcgaaa gacgaaaaa gagaaagat aaagacaag gagaaagat aaatcgaag aaagaagaa gacggagat gtgtcgaat aaagaagtt gaagaaacg gaaccggaa gaaaccgac gacgaaaaa gcacaagaa caaagagat aaagatagg caagccagg gcggaggcg agtctgaga gagagggag aaggaggta caaaggact ctagcgacg catatgagg gaccgagat aaggagagg gagcaacac aaacgggac gaggctata caacatttt aatgcgtta ttggccgat ttggtgcgc aatcccgag ctgagctgg cgcgaggtt aagcgcatt ttgcgaaaa gaccaccgt tgggacttg gctgattcg ctgtcgcgt gaagataag gaaaaatta ttcaatgaa cacattgaa catttgttg aggaagaag agagagaag ttccgcgag ttattggat gaaaccccc gatgttact ttaaccagt agttggaaa gaaattaaa aaaattata aaagaggat ccccgatat acgaaattt gcgtcaagc gaacggtgt gaacgtgaa tttaaggat tatctcaaa gataaacta ataaccgcc aaaggacaa ttcaaggaa ttgttacag gaaactaaa cttattaca cataaatcg ttatctaat ctcagagag aatcaagga tttatgcaa gaaattgag gatatttta aagaatgat aagagatat cttgtcttg gatcatatt ccgcaagag aggactcag ttgattctg aattatttg gaagaactc gatagaagg ggaccacct ccgccacct acggctagt gaacctaat aggagatct attaaatag
- >TC004501 RC
atggaagaa acagacgtc tccatggaa tacgacggc gccgacgtc gacggcgtc gcccaagag gaatacgaa gaggacgga ggcttcgaa aacgacatt gaactcgga gatgacttc caaatgcga ggccgaggt ccttcaggg ttcaggggc cgcggacgc gcgtggccg ggtcctcca ggtccgcca ggaccgcgt ggagaaggt ccccggttt cgaggaagg ggatttggg cccggtggc cctcctccg tttcgtgga cgtggagga ccacctcct ccgatgttt ggacgtggg ggacctccc agaaaccca ccaccaggg cagggattc aatggacct ccgaatttt aatgggcct aactgggga ggaccgatg gggcccccg gggacgatg ggtggaccg cccaatatg atgggaccc aatggaccg ccgttcgga ccaccgggc atgatgggg gcgcccccg ggtggaccc atgggcgct cagggcggg acgaccaat gggcagttt ccaccagta gggcaaaat cccacaaca gggttagat gcaaatagt gaaatttgg gtagagact aaaacctca gaaggcaag tcatatttt tataatgct cgtactaga gaaaccact tggactaag ccagaaggt cctaatgta aaagtaatc tctcaggat caagtggaa gcaatggca caagcggcc accacaggt ttagcccaa ggaacgtca actgcagct caggcggcg ttagcgcag gctaacgtt tcaaataaa cctgaagtt gagtcagag gaatcgaaa ggcgatgcc aaacaagct gggaacgca atgcctcaa aacatgtta gcgggtcct ccgccaggt atgccccca tttgggggt cccccaggt cagtttgga gcgcctcca ttcggctta ccaccgcca ggattccaa gggacatgg ccggctcca ggtcaagca gcatgggca ggggggcca ccaggggct gctccgtgg ggtatgccg ccaggtctt cttacaggt ttaccagga gctatccca gcgattgac gaagcagcg gttttggct aaagtcgat ccggaaatc atcgccaaa gcgtccgaa tggagcgaa cacaaagca cccgacgga cgtttctat tattacaac gccaagaaa ggggaatcg gtttgggaa aaaccgcaa gctcttaag gatttggaa actgcgaaa ctggctgca gctcaaggg atttcaaca agaccggga accgaagtt atagctgct gttgagaca ggtaagcca aacgctgca gttgtccaa gtggccaat ggggacgca gtgaaagac attgtcata aaggaggaa gatgacaag atcaaaaag ccccaagac gaaacgaaa aagaagaag gaagaagaa gaaaaaaag gaaactaaa agtcaagat aaatcgcgc ccagtgtca agtaccccc gtcccagga accccatgg tgtgttgtg tggaccggc gatggaaga gttttcttc tacaaccca tcgtcaagg acatccgtt tgggagcgt cctgatgag ctaataaaa cgaacagat gtggataaa atggtagcg acacctccc gattccgtc ggatctcaa accaaacca gacgctgaa agtccgtcg aaaaaacgc gtatccgac gacagcgac agtgaggaa gaaacccca gctaaaaaa ccgaaaaaa gaagaaata accagtacg aacggtacc actccacaa tcagctcgt aaaatcgat attggcaaa gaagcagcc attgaagcg gaagtacgt gcggccaaa gaacgtgct attgtccca ctggaaact cgaattaaa tcatttaaa gaaatgctg gccgagaaa aacgtttcc gcgtttagt acttgggaa aaagaacta cataaaatt gttttcgat acacggtat ctccttcta acgtccaag gaacgcaaa caagtgttt gaaaagtac gttaaagaa cgagccgag gaagagcga agagagaaa cggaataag ttgagagag aagaaggac gcgtttagg aaactgttg agtgagtcg catctacac ggcaaatcg tcgtttagt gacttcgca cagaagttc gcaaaggac gaacgattt aaaggggtt gagaaaatg cgcgaacgg gaaagtctc ttcaatgaa tatttaatc gaagtaaga aagcgcgaa aaagaagaa aagaatcag cgacgagaa caggtgaga atgagatgt tttttatcc caaacatac gaaaaagta acgatgtaa
- >TC004501 RA
acaaacatt tttcaaatt tgtgattga ttcgtaata aaataataa ttggaaaat gtttgaaga ctcactaat gttgccttt tttccagct ctaatattt ttttcagga tcgagtgac cagttagtg ataatttag tgataaaac ataaattta aataaaacc ctgttggtt tctcaaact tgatgatat acgtgtttg caggtaaag aaagatttc tttgcaatg ctacgcgaa cactctgat atcgaccgt cattcccac tgggccgat gtgaaacga aaagttgat tcggacgct cgatacaaa gccgttgat tcgtccgga caacgtgaa gattggttc agagaatat tgcaagata cttaaagaa gagaaaaag aaagcgaaa gagaaagat cgcgaacac aaacgtgat aaagaaaag cataagaag aaggataaa gataaggat aaggaacgt cattcgaaa gacgaaaaa gagaaagat aaagacaag gagaaagat aaatcgaag aaagaagaa gacggagat gtgtcgaat aaagaagtt gaagaaacg gaaccggaa gaaaccgac gacgaaaaa gcacaagaa caaagagat aaagatagg caagccagg gcggaggcg agtctgaga gagagggag aaggaggta caaaggact ctagcgacg catatgagg gaccgagat aaggagagg gagcaacac aaacgggac gaggctata caacatttt aatgcgtta ttggccgat ttggtgcgc aatcccgag ctgagctgg cgcgaggtt aagcgcatt ttgcgaaaa gaccaccgt tgggacttg gctgattcg ctgtcgcgt gaagataag gaaaaatta ttcaatgaa cacattgaa catttgttg aggaagaag agagagaag ttccgcgag ttattggat gaaaccccc gatgttact ttaaccagt agttggaaa gaaattaaa aaaattata aaagaggat ccccgatat acgaaattt gcgtcaagc gaacggtgt gaacgtgaa tttaaggat tatctcaaa gataaacta ataaccgcc aaaggacaa ttcaaggaa ttgttacag gaaactaaa cttattaca cataaatcg ttatctaat ctcagagag aatcaagga tttatgcaa gaaattgag gatatttta aagaatgat aagagatat cttgtcttg gatcatatt ccgcaagag aggactcag ttgattctg aattatttg gaagaactc gatagaagg ggaccacct ccgccacct acggctagt gaacctaat aggagatct attaaatag ggaattact ttttatcgt atttattat attgtctgg atgctgaag ttgtgcatt ttattgtct tagtggctc cagttgtag ctatttgaa ctgaatgtg ttacacgat taattaaat ttgatttta tactgtaat agaataaaa actccgtat ttac
- >TC004501 RB
cataaaccg aagaagtgc atttgttga cgccatttt ccgaaaaat aaatcttaa ttgactcta aatggctaa attgattga aattaaata aaacacaat ggaagaaac agacgtctc catggaata cgacggcgc cgacgtcga cggcgtcgc ccaagagga atacgaaga ggacggagg cttcgaaaa cgacattga actcggaga tgacttcca aatgcgagg ccgaggtcc ttcagggtt caggggccg cggacgcgc gtggccggg tcctccagg tccgccagg accgcgtgg agaaggtcc ccggtttcg aggaagggg atttgggcc cggtggccc tcctccgtt tcgtggacg tggaggacc acctcctcc gatgtttgg acgtggggg acctcccag aaacccacc accagggca gggattcaa tggacctcc gaattttaa tgggcctaa ctggggagg accgatggg gcccccggg gacgatggg tggaccgcc caatatgat gggacccaa tggaccgcc gttcggacc accgggcat gatgggggc gcccccggg tggacccat gggcgctca gggcgggac gaccaatgg gcagtttcc accagtagg gcaaaatcc cacaacagg gttagatgc aaatagtga aatttgggt agagactaa aacctcaga aggcaagtc atattttta taatgctcg tactagaga aaccacttg gactaagcc agaaggtcc taatgtaaa agtaatctc tcaggatca agtggaagc aatggcaca agcggccac cacaggttt agcccaagg aacgtcaac tgcagctca ggcggcgtt agcgcaggc taacgtttc aaataaacc tgaagttga gtcagagga atcgaaagg cgatgccaa acaagctgg gaacgcaat gcctcaaaa catgttagc gggtcctcc gccaggtat gcccccatt tgggggtcc cccaggtca gtttggagc gcctccatt cggcttacc accgccagg attccaagg gacatggcc ggctccagg tcaagcagc atgggcagg ggggccacc aggggctgc tccgtgggg tatgccgcc aggtcttct tacaggttt accaggagc tatcccagc gattgacga agcagcggt tttggctaa agtcgatcc ggaaatcat cgccaaagc gtccgaatg gagcgaaca caaagcacc cgacggacg tttctatta ttacaacgc caagaaagg ggaatcggt ttgggaaaa accgcaagc tcttaagga tttggaaac tgcgaaact ggctgcagc tcaagggat ttcaacaag accgggaac cgaagttat agctgctgt tgagacagg taagccaaa cgctgcagt tgtccaagt ggccaatgg ggacgcagt gaaagacat tgtcataaa ggaggaaga tgacaagat caaaaagcc ccaagacga aacgaaaaa gaagaagga agaagaaga aaaaaagga aactaaaag tcaagataa atcgcgccc agtgtcaag tacccccgt cccaggaac cccatggtg tgttgtgtg gaccggcga tggaagagt tttcttcta caacccatc gtcaaggac atccgtttg ggagcgtcc tgatgagct aataaaacg aacagatgt ggataaaat ggtagcgac acctcccga ttccgtcgg atctcaaac caaaccaga cgctgaaag tccgtcgaa aaaacgcgt atccgacga cagcgacag tgaggaaga aaccccagc taaaaaacc gaaaaaaga agaaataac cagtacgaa cggtaccac tccacaatc agctcgtaa aatcgatat tggcaaaga agcagccat tgaagcgga agtacgtgc ggccaaaga acgtgctat tgtcccact ggaaactcg aattaaatc atttaaaga aatgctggc cgagaaaaa cgtttccgc gtttagtac ttgggaaaa agaactaca taaaattgt tttcgatac acggtatct ccttctaac gtccaagga acgcaaaca agtgtttga aaagtacgt taaagaacg agccgagga agagcgaag agagaaacg gaataagtt gagagagaa gaaggacgc gtttaggaa actgttgag tgagtcgca tctacacgg caaatcgtc gtttagtga cttcgcaca gaagttcgc aaaggacga acgatttaa aggggttga gaaaatgcg cgaacggga aagtctctt caatgaata tttaatcga agtaagaaa gcgcgaaaa agaagaaaa gaatcagcg acgagaaca ggtaaagaa agatttctt tgcaatgct acgcgaaca ctctgatat cgaccgtca ttcccactg ggccgatgt gaaacgaaa agttgattc ggacgctcg atacaaagc cgttgattc gtccggaca acgtgaaga ttggttcag agaatattg caagatact taaagaaga gaaaaagaa agcgaaaga gaaagatcg cgaacacaa acgtgataa agaaaagca taagaagaa ggataaaga taaggataa ggaacgtca ttcgaaaga cgaaaaaga gaaagataa agacaagga gaaagataa atcgaagaa agaagaaga cggagatgt gtcgaataa agaagttga agaaacgga accggaaga aaccgacga cgaaaaagc acaagaaca aagagataa agataggca agccagggc ggaggcgag tctgagaga gagggagaa ggaggtaca aaggactct agcgacgca tatgaggga ccgagataa ggagaggga gcaacacaa acgggacga ggctataca acattttaa tgcgttatt ggccgattt ggtgcgcaa tcccgagct gagctggcg cgaggttaa gcgcatttt gcgaaaaga ccaccgttg ggacttggc tgattcgct gtcgcgtga agataagga aaaattatt caatgaaca cattgaaca tttgttgag gaagaagag agagaagtt ccgcgagtt attggatga aacccccga tgttacttt aaccagtag ttggaaaga aattaaaaa aattataaa agaggatcc ccgatatac gaaatttgc gtcaagcga acggtgtga acgtgaatt taaggatta tctcaaaga taaactaat aaccgccaa aggacaatt caaggaatt gttacagga aactaaact tattacaca taaatcgtt atctaatct cagagagaa tcaaggatt tatgcaaga aattgagga tattttaaa gaatgataa gagatatct tgtcttgga tcatattcc gcaagagag gactcagtt gattctgaa ttatttgga agaactcga tagaagggg accacctcc gccacctac ggctagtga acctaatag gagatctat taaataggg aattacttt ttatcgtat ttattatat tgtctggat gctgaagtt gtgcatttt attgtctta gtggctcca gttgtagct atttgaact gaatgtgtt acacgatta attaaattt gattttata ctgtaatag aataaaaac tccgtattt ac
- >TC004501 RC
cataaaccg aagaagtgc atttgttga cgccatttt ccgaaaaat aaatcttaa ttgactcta aatggctaa attgattga aattaaata aaacacaat ggaagaaac agacgtctc catggaata cgacggcgc cgacgtcga cggcgtcgc ccaagagga atacgaaga ggacggagg cttcgaaaa cgacattga actcggaga tgacttcca aatgcgagg ccgaggtcc ttcagggtt caggggccg cggacgcgc gtggccggg tcctccagg tccgccagg accgcgtgg agaaggtcc ccggtttcg aggaagggg atttgggcc cggtggccc tcctccgtt tcgtggacg tggaggacc acctcctcc gatgtttgg acgtggggg acctcccag aaacccacc accagggca gggattcaa tggacctcc gaattttaa tgggcctaa ctggggagg accgatggg gcccccggg gacgatggg tggaccgcc caatatgat gggacccaa tggaccgcc gttcggacc accgggcat gatgggggc gcccccggg tggacccat gggcgctca gggcgggac gaccaatgg gcagtttcc accagtagg gcaaaatcc cacaacagg gttagatgc aaatagtga aatttgggt agagactaa aacctcaga aggcaagtc atattttta taatgctcg tactagaga aaccacttg gactaagcc agaaggtcc taatgtaaa agtaatctc tcaggatca agtggaagc aatggcaca agcggccac cacaggttt agcccaagg aacgtcaac tgcagctca ggcggcgtt agcgcaggc taacgtttc aaataaacc tgaagttga gtcagagga atcgaaagg cgatgccaa acaagctgg gaacgcaat gcctcaaaa catgttagc gggtcctcc gccaggtat gcccccatt tgggggtcc cccaggtca gtttggagc gcctccatt cggcttacc accgccagg attccaagg gacatggcc ggctccagg tcaagcagc atgggcagg ggggccacc aggggctgc tccgtgggg tatgccgcc aggtcttct tacaggttt accaggagc tatcccagc gattgacga agcagcggt tttggctaa agtcgatcc ggaaatcat cgccaaagc gtccgaatg gagcgaaca caaagcacc cgacggacg tttctatta ttacaacgc caagaaagg ggaatcggt ttgggaaaa accgcaagc tcttaagga tttggaaac tgcgaaact ggctgcagc tcaagggat ttcaacaag accgggaac cgaagttat agctgctgt tgagacagg taagccaaa cgctgcagt tgtccaagt ggccaatgg ggacgcagt gaaagacat tgtcataaa ggaggaaga tgacaagat caaaaagcc ccaagacga aacgaaaaa gaagaagga agaagaaga aaaaaagga aactaaaag tcaagataa atcgcgccc agtgtcaag tacccccgt cccaggaac cccatggtg tgttgtgtg gaccggcga tggaagagt tttcttcta caacccatc gtcaaggac atccgtttg ggagcgtcc tgatgagct aataaaacg aacagatgt ggataaaat ggtagcgac acctcccga ttccgtcgg atctcaaac caaaccaga cgctgaaag tccgtcgaa aaaacgcgt atccgacga cagcgacag tgaggaaga aaccccagc taaaaaacc gaaaaaaga agaaataac cagtacgaa cggtaccac tccacaatc agctcgtaa aatcgatat tggcaaaga agcagccat tgaagcgga agtacgtgc ggccaaaga acgtgctat tgtcccact ggaaactcg aattaaatc atttaaaga aatgctggc cgagaaaaa cgtttccgc gtttagtac ttgggaaaa agaactaca taaaattgt tttcgatac acggtatct ccttctaac gtccaagga acgcaaaca agtgtttga aaagtacgt taaagaacg agccgagga agagcgaag agagaaacg gaataagtt gagagagaa gaaggacgc gtttaggaa actgttgag tgagtcgca tctacacgg caaatcgtc gtttagtga cttcgcaca gaagttcgc aaaggacga acgatttaa aggggttga gaaaatgcg cgaacggga aagtctctt caatgaata tttaatcga agtaagaaa gcgcgaaaa agaagaaaa gaatcagcg acgagaaca ggtgagaat gagatgttt tttatccca aacatacga aaaagtaac gatgtaaca agttcctta cttgttaca ttatcggct tgcaatagt agccttttt tagtaagtg ttggtgatc taattttaa ttatttatg ttaatcgat gcgaaccta atcacgcgc acaatacta aaaattatt tacaggttt tcagcactc ggtatgtag atgtgttag attaatttt atttaagga ttgttgaaa attgtgttt ttgccacaa tttttataa cagcgctca ttaatatta taaagcaag agttcatta agtccgatt aaatcaatt ccactttgt agcaactta ctgaactgt tgttaccaa agtttgaca tttgtaacc aaccacaaa ccatttcac ttatttttt agtgcaatt atgtttgtt ttggtttgt tgttggtac ggttgctgt ttcaatgcc tatatttta cgaccaatc aagaacgct aacagattg taaatgatg agtctctta tccagcttc attactttt tcaagagat gtggagtac gggtgaaac acgatttct tccataacc cgaggctag tgtgcatcg agtcagaga agtcgtccc gaggtgtgt gtcagcgct ccttgccgg caatggggc taattttta tttaacggt acttaacct acaagttag ttgttacat ttttaagaa caaaaatca agtaattag taaagattt ttcgtacaa atacaacac aaatcgaaa g
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