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Gene information
General information
TC004501 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3684548..3691317
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004501
    iBeetle screen iB_10670

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna bent - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs segment potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004501 RA
      MLREHSDID RHSHWADVK RKVDSDARY KAVDSSGQR 
      EDWFREYCK ILKEEKKKA KEKDREHKR DKEKHKKKD 
      KDKDKERHS KDEKEKDKD KEKDKSKKE EDGDVSNKE 
      VEETEPEET DDEKAQEQR DKDRQARAE ASLREREKE 
      VQRTLATHM RDRDKEREQ HKRDEAIQH FNALLADLV 
      RNPELSWRE VKRILRKDH RWDLADSLS REDKEKLFN 
      EHIEHLLRK KREKFRELL DETPDVTLT SSWKEIKKI 
      IKEDPRYTK FASSERCER EFKDYLKDK LITAKGQFK 
      ELLQETKLI THKSLSNLR ENQGFMQEI EDILKNDKR 
      YLVLDHIPQ ERTQLILNY LEELDRRGP PPPPTASEP 
      NRRSIK
    • >TC004501 RB
      MEETDVSME YDGADVDGV AQEEYEEDG GFENDIELG 
      DDFQMRGRG PSGFRGRGR AWPGPPGPP GPRGEGPRF 
      RGRGFGPGG PPPFRGRGG PPPPMFGRG GPPRNPPPG 
      QGFNGPPNF NGPNWGGPM GPPGTMGGP PNMMGPNGP 
      PFGPPGMMG APPGGPMGA QGGTTNGQF PPVGQNPTT 
      GLDANSEIW VETKTSEGK SYFYNARTR ETTWTKPEG 
      PNVKVISQD QVEAMAQAA TTGLAQGTS TAAQAALAQ 
      ANVSNKPEV ESEESKGDA KQAGNAMPQ NMLAGPPPG 
      MPPFGGPPG QFGAPPFGL PPPGFQGTW PAPGQAAWA 
      GGPPGAAPW GMPPGLLTG LPGAIPAID EAAVLAKVD 
      PEIIAKASE WSEHKAPDG RFYYYNAKK GESVWEKPQ 
      ALKDLETAK LAAAQGIST RPGTEVIAA VETGKPNAA 
      VVQVANGDA VKDIVIKEE DDKIKKPQD ETKKKKEEE 
      EKKETKSQD KSRPVSSTP VPGTPWCVV WTGDGRVFF 
      YNPSSRTSV WERPDELIK RTDVDKMVA TPPDSVGSQ 
      TKPDAESPS KKRVSDDSD SEEETPAKK PKKEEITST 
      NGTTPQSAR KIDIGKEAA IEAEVRAAK ERAIVPLET 
      RIKSFKEML AEKNVSAFS TWEKELHKI VFDTRYLLL 
      TSKERKQVF EKYVKERAE EERREKRNK LREKKDAFR 
      KLLSESHLH GKSSFSDFA QKFAKDERF KGVEKMRER 
      ESLFNEYLI EVRKREKEE KNQRREQVK KDFFAMLRE 
      HSDIDRHSH WADVKRKVD SDARYKAVD SSGQREDWF 
      REYCKILKE EKKKAKEKD REHKRDKEK HKKKDKDKD 
      KERHSKDEK EKDKDKEKD KSKKEEDGD VSNKEVEET 
      EPEETDDEK AQEQRDKDR QARAEASLR EREKEVQRT 
      LATHMRDRD KEREQHKRD EAIQHFNAL LADLVRNPE 
      LSWREVKRI LRKDHRWDL ADSLSREDK EKLFNEHIE 
      HLLRKKREK FRELLDETP DVTLTSSWK EIKKIIKED 
      PRYTKFASS ERCEREFKD YLKDKLITA KGQFKELLQ 
      ETKLITHKS LSNLRENQG FMQEIEDIL KNDKRYLVL 
      DHIPQERTQ LILNYLEEL DRRGPPPPP TASEPNRRS 
      IK
    • >TC004501 RC
      MEETDVSME YDGADVDGV AQEEYEEDG GFENDIELG 
      DDFQMRGRG PSGFRGRGR AWPGPPGPP GPRGEGPRF 
      RGRGFGPGG PPPFRGRGG PPPPMFGRG GPPRNPPPG 
      QGFNGPPNF NGPNWGGPM GPPGTMGGP PNMMGPNGP 
      PFGPPGMMG APPGGPMGA QGGTTNGQF PPVGQNPTT 
      GLDANSEIW VETKTSEGK SYFYNARTR ETTWTKPEG 
      PNVKVISQD QVEAMAQAA TTGLAQGTS TAAQAALAQ 
      ANVSNKPEV ESEESKGDA KQAGNAMPQ NMLAGPPPG 
      MPPFGGPPG QFGAPPFGL PPPGFQGTW PAPGQAAWA 
      GGPPGAAPW GMPPGLLTG LPGAIPAID EAAVLAKVD 
      PEIIAKASE WSEHKAPDG RFYYYNAKK GESVWEKPQ 
      ALKDLETAK LAAAQGIST RPGTEVIAA VETGKPNAA 
      VVQVANGDA VKDIVIKEE DDKIKKPQD ETKKKKEEE 
      EKKETKSQD KSRPVSSTP VPGTPWCVV WTGDGRVFF 
      YNPSSRTSV WERPDELIK RTDVDKMVA TPPDSVGSQ 
      TKPDAESPS KKRVSDDSD SEEETPAKK PKKEEITST 
      NGTTPQSAR KIDIGKEAA IEAEVRAAK ERAIVPLET 
      RIKSFKEML AEKNVSAFS TWEKELHKI VFDTRYLLL 
      TSKERKQVF EKYVKERAE EERREKRNK LREKKDAFR 
      KLLSESHLH GKSSFSDFA QKFAKDERF KGVEKMRER 
      ESLFNEYLI EVRKREKEE KNQRREQVR MRCFLSQTY 
      EKVTM
    CDS sequence
    • >TC004501 RA
      atgctacgc gaacactct gatatcgac cgtcattcc 
      cactgggcc gatgtgaaa cgaaaagtt gattcggac 
      gctcgatac aaagccgtt gattcgtcc ggacaacgt 
      gaagattgg ttcagagaa tattgcaag atacttaaa 
      gaagagaaa aagaaagcg aaagagaaa gatcgcgaa 
      cacaaacgt gataaagaa aagcataag aagaaggat 
      aaagataag gataaggaa cgtcattcg aaagacgaa 
      aaagagaaa gataaagac aaggagaaa gataaatcg 
      aagaaagaa gaagacgga gatgtgtcg aataaagaa 
      gttgaagaa acggaaccg gaagaaacc gacgacgaa 
      aaagcacaa gaacaaaga gataaagat aggcaagcc 
      agggcggag gcgagtctg agagagagg gagaaggag 
      gtacaaagg actctagcg acgcatatg agggaccga 
      gataaggag agggagcaa cacaaacgg gacgaggct 
      atacaacat tttaatgcg ttattggcc gatttggtg 
      cgcaatccc gagctgagc tggcgcgag gttaagcgc 
      attttgcga aaagaccac cgttgggac ttggctgat 
      tcgctgtcg cgtgaagat aaggaaaaa ttattcaat 
      gaacacatt gaacatttg ttgaggaag aagagagag 
      aagttccgc gagttattg gatgaaacc cccgatgtt 
      actttaacc agtagttgg aaagaaatt aaaaaaatt 
      ataaaagag gatccccga tatacgaaa tttgcgtca 
      agcgaacgg tgtgaacgt gaatttaag gattatctc 
      aaagataaa ctaataacc gccaaagga caattcaag 
      gaattgtta caggaaact aaacttatt acacataaa 
      tcgttatct aatctcaga gagaatcaa ggatttatg 
      caagaaatt gaggatatt ttaaagaat gataagaga 
      tatcttgtc ttggatcat attccgcaa gagaggact 
      cagttgatt ctgaattat ttggaagaa ctcgataga 
      aggggacca cctccgcca cctacggct agtgaacct 
      aataggaga tctattaaa tag
    • >TC004501 RB
      atggaagaa acagacgtc tccatggaa tacgacggc 
      gccgacgtc gacggcgtc gcccaagag gaatacgaa 
      gaggacgga ggcttcgaa aacgacatt gaactcgga 
      gatgacttc caaatgcga ggccgaggt ccttcaggg 
      ttcaggggc cgcggacgc gcgtggccg ggtcctcca 
      ggtccgcca ggaccgcgt ggagaaggt ccccggttt 
      cgaggaagg ggatttggg cccggtggc cctcctccg 
      tttcgtgga cgtggagga ccacctcct ccgatgttt 
      ggacgtggg ggacctccc agaaaccca ccaccaggg 
      cagggattc aatggacct ccgaatttt aatgggcct 
      aactgggga ggaccgatg gggcccccg gggacgatg 
      ggtggaccg cccaatatg atgggaccc aatggaccg 
      ccgttcgga ccaccgggc atgatgggg gcgcccccg 
      ggtggaccc atgggcgct cagggcggg acgaccaat 
      gggcagttt ccaccagta gggcaaaat cccacaaca 
      gggttagat gcaaatagt gaaatttgg gtagagact 
      aaaacctca gaaggcaag tcatatttt tataatgct 
      cgtactaga gaaaccact tggactaag ccagaaggt 
      cctaatgta aaagtaatc tctcaggat caagtggaa 
      gcaatggca caagcggcc accacaggt ttagcccaa 
      ggaacgtca actgcagct caggcggcg ttagcgcag 
      gctaacgtt tcaaataaa cctgaagtt gagtcagag 
      gaatcgaaa ggcgatgcc aaacaagct gggaacgca 
      atgcctcaa aacatgtta gcgggtcct ccgccaggt 
      atgccccca tttgggggt cccccaggt cagtttgga 
      gcgcctcca ttcggctta ccaccgcca ggattccaa 
      gggacatgg ccggctcca ggtcaagca gcatgggca 
      ggggggcca ccaggggct gctccgtgg ggtatgccg 
      ccaggtctt cttacaggt ttaccagga gctatccca 
      gcgattgac gaagcagcg gttttggct aaagtcgat 
      ccggaaatc atcgccaaa gcgtccgaa tggagcgaa 
      cacaaagca cccgacgga cgtttctat tattacaac 
      gccaagaaa ggggaatcg gtttgggaa aaaccgcaa 
      gctcttaag gatttggaa actgcgaaa ctggctgca 
      gctcaaggg atttcaaca agaccggga accgaagtt 
      atagctgct gttgagaca ggtaagcca aacgctgca 
      gttgtccaa gtggccaat ggggacgca gtgaaagac 
      attgtcata aaggaggaa gatgacaag atcaaaaag 
      ccccaagac gaaacgaaa aagaagaag gaagaagaa 
      gaaaaaaag gaaactaaa agtcaagat aaatcgcgc 
      ccagtgtca agtaccccc gtcccagga accccatgg 
      tgtgttgtg tggaccggc gatggaaga gttttcttc 
      tacaaccca tcgtcaagg acatccgtt tgggagcgt 
      cctgatgag ctaataaaa cgaacagat gtggataaa 
      atggtagcg acacctccc gattccgtc ggatctcaa 
      accaaacca gacgctgaa agtccgtcg aaaaaacgc 
      gtatccgac gacagcgac agtgaggaa gaaacccca 
      gctaaaaaa ccgaaaaaa gaagaaata accagtacg 
      aacggtacc actccacaa tcagctcgt aaaatcgat 
      attggcaaa gaagcagcc attgaagcg gaagtacgt 
      gcggccaaa gaacgtgct attgtccca ctggaaact 
      cgaattaaa tcatttaaa gaaatgctg gccgagaaa 
      aacgtttcc gcgtttagt acttgggaa aaagaacta 
      cataaaatt gttttcgat acacggtat ctccttcta 
      acgtccaag gaacgcaaa caagtgttt gaaaagtac 
      gttaaagaa cgagccgag gaagagcga agagagaaa 
      cggaataag ttgagagag aagaaggac gcgtttagg 
      aaactgttg agtgagtcg catctacac ggcaaatcg 
      tcgtttagt gacttcgca cagaagttc gcaaaggac 
      gaacgattt aaaggggtt gagaaaatg cgcgaacgg 
      gaaagtctc ttcaatgaa tatttaatc gaagtaaga 
      aagcgcgaa aaagaagaa aagaatcag cgacgagaa 
      caggtaaag aaagatttc tttgcaatg ctacgcgaa 
      cactctgat atcgaccgt cattcccac tgggccgat 
      gtgaaacga aaagttgat tcggacgct cgatacaaa 
      gccgttgat tcgtccgga caacgtgaa gattggttc 
      agagaatat tgcaagata cttaaagaa gagaaaaag 
      aaagcgaaa gagaaagat cgcgaacac aaacgtgat 
      aaagaaaag cataagaag aaggataaa gataaggat 
      aaggaacgt cattcgaaa gacgaaaaa gagaaagat 
      aaagacaag gagaaagat aaatcgaag aaagaagaa 
      gacggagat gtgtcgaat aaagaagtt gaagaaacg 
      gaaccggaa gaaaccgac gacgaaaaa gcacaagaa 
      caaagagat aaagatagg caagccagg gcggaggcg 
      agtctgaga gagagggag aaggaggta caaaggact 
      ctagcgacg catatgagg gaccgagat aaggagagg 
      gagcaacac aaacgggac gaggctata caacatttt 
      aatgcgtta ttggccgat ttggtgcgc aatcccgag 
      ctgagctgg cgcgaggtt aagcgcatt ttgcgaaaa 
      gaccaccgt tgggacttg gctgattcg ctgtcgcgt 
      gaagataag gaaaaatta ttcaatgaa cacattgaa 
      catttgttg aggaagaag agagagaag ttccgcgag 
      ttattggat gaaaccccc gatgttact ttaaccagt 
      agttggaaa gaaattaaa aaaattata aaagaggat 
      ccccgatat acgaaattt gcgtcaagc gaacggtgt 
      gaacgtgaa tttaaggat tatctcaaa gataaacta 
      ataaccgcc aaaggacaa ttcaaggaa ttgttacag 
      gaaactaaa cttattaca cataaatcg ttatctaat 
      ctcagagag aatcaagga tttatgcaa gaaattgag 
      gatatttta aagaatgat aagagatat cttgtcttg 
      gatcatatt ccgcaagag aggactcag ttgattctg 
      aattatttg gaagaactc gatagaagg ggaccacct 
      ccgccacct acggctagt gaacctaat aggagatct 
      attaaatag
    • >TC004501 RC
      atggaagaa acagacgtc tccatggaa tacgacggc 
      gccgacgtc gacggcgtc gcccaagag gaatacgaa 
      gaggacgga ggcttcgaa aacgacatt gaactcgga 
      gatgacttc caaatgcga ggccgaggt ccttcaggg 
      ttcaggggc cgcggacgc gcgtggccg ggtcctcca 
      ggtccgcca ggaccgcgt ggagaaggt ccccggttt 
      cgaggaagg ggatttggg cccggtggc cctcctccg 
      tttcgtgga cgtggagga ccacctcct ccgatgttt 
      ggacgtggg ggacctccc agaaaccca ccaccaggg 
      cagggattc aatggacct ccgaatttt aatgggcct 
      aactgggga ggaccgatg gggcccccg gggacgatg 
      ggtggaccg cccaatatg atgggaccc aatggaccg 
      ccgttcgga ccaccgggc atgatgggg gcgcccccg 
      ggtggaccc atgggcgct cagggcggg acgaccaat 
      gggcagttt ccaccagta gggcaaaat cccacaaca 
      gggttagat gcaaatagt gaaatttgg gtagagact 
      aaaacctca gaaggcaag tcatatttt tataatgct 
      cgtactaga gaaaccact tggactaag ccagaaggt 
      cctaatgta aaagtaatc tctcaggat caagtggaa 
      gcaatggca caagcggcc accacaggt ttagcccaa 
      ggaacgtca actgcagct caggcggcg ttagcgcag 
      gctaacgtt tcaaataaa cctgaagtt gagtcagag 
      gaatcgaaa ggcgatgcc aaacaagct gggaacgca 
      atgcctcaa aacatgtta gcgggtcct ccgccaggt 
      atgccccca tttgggggt cccccaggt cagtttgga 
      gcgcctcca ttcggctta ccaccgcca ggattccaa 
      gggacatgg ccggctcca ggtcaagca gcatgggca 
      ggggggcca ccaggggct gctccgtgg ggtatgccg 
      ccaggtctt cttacaggt ttaccagga gctatccca 
      gcgattgac gaagcagcg gttttggct aaagtcgat 
      ccggaaatc atcgccaaa gcgtccgaa tggagcgaa 
      cacaaagca cccgacgga cgtttctat tattacaac 
      gccaagaaa ggggaatcg gtttgggaa aaaccgcaa 
      gctcttaag gatttggaa actgcgaaa ctggctgca 
      gctcaaggg atttcaaca agaccggga accgaagtt 
      atagctgct gttgagaca ggtaagcca aacgctgca 
      gttgtccaa gtggccaat ggggacgca gtgaaagac 
      attgtcata aaggaggaa gatgacaag atcaaaaag 
      ccccaagac gaaacgaaa aagaagaag gaagaagaa 
      gaaaaaaag gaaactaaa agtcaagat aaatcgcgc 
      ccagtgtca agtaccccc gtcccagga accccatgg 
      tgtgttgtg tggaccggc gatggaaga gttttcttc 
      tacaaccca tcgtcaagg acatccgtt tgggagcgt 
      cctgatgag ctaataaaa cgaacagat gtggataaa 
      atggtagcg acacctccc gattccgtc ggatctcaa 
      accaaacca gacgctgaa agtccgtcg aaaaaacgc 
      gtatccgac gacagcgac agtgaggaa gaaacccca 
      gctaaaaaa ccgaaaaaa gaagaaata accagtacg 
      aacggtacc actccacaa tcagctcgt aaaatcgat 
      attggcaaa gaagcagcc attgaagcg gaagtacgt 
      gcggccaaa gaacgtgct attgtccca ctggaaact 
      cgaattaaa tcatttaaa gaaatgctg gccgagaaa 
      aacgtttcc gcgtttagt acttgggaa aaagaacta 
      cataaaatt gttttcgat acacggtat ctccttcta 
      acgtccaag gaacgcaaa caagtgttt gaaaagtac 
      gttaaagaa cgagccgag gaagagcga agagagaaa 
      cggaataag ttgagagag aagaaggac gcgtttagg 
      aaactgttg agtgagtcg catctacac ggcaaatcg 
      tcgtttagt gacttcgca cagaagttc gcaaaggac 
      gaacgattt aaaggggtt gagaaaatg cgcgaacgg 
      gaaagtctc ttcaatgaa tatttaatc gaagtaaga 
      aagcgcgaa aaagaagaa aagaatcag cgacgagaa 
      caggtgaga atgagatgt tttttatcc caaacatac 
      gaaaaagta acgatgtaa
    mRNA sequence
    • >TC004501 RA
      acaaacatt tttcaaatt tgtgattga ttcgtaata 
      aaataataa ttggaaaat gtttgaaga ctcactaat 
      gttgccttt tttccagct ctaatattt ttttcagga 
      tcgagtgac cagttagtg ataatttag tgataaaac 
      ataaattta aataaaacc ctgttggtt tctcaaact 
      tgatgatat acgtgtttg caggtaaag aaagatttc 
      tttgcaatg ctacgcgaa cactctgat atcgaccgt 
      cattcccac tgggccgat gtgaaacga aaagttgat 
      tcggacgct cgatacaaa gccgttgat tcgtccgga 
      caacgtgaa gattggttc agagaatat tgcaagata 
      cttaaagaa gagaaaaag aaagcgaaa gagaaagat 
      cgcgaacac aaacgtgat aaagaaaag cataagaag 
      aaggataaa gataaggat aaggaacgt cattcgaaa 
      gacgaaaaa gagaaagat aaagacaag gagaaagat 
      aaatcgaag aaagaagaa gacggagat gtgtcgaat 
      aaagaagtt gaagaaacg gaaccggaa gaaaccgac 
      gacgaaaaa gcacaagaa caaagagat aaagatagg 
      caagccagg gcggaggcg agtctgaga gagagggag 
      aaggaggta caaaggact ctagcgacg catatgagg 
      gaccgagat aaggagagg gagcaacac aaacgggac 
      gaggctata caacatttt aatgcgtta ttggccgat 
      ttggtgcgc aatcccgag ctgagctgg cgcgaggtt 
      aagcgcatt ttgcgaaaa gaccaccgt tgggacttg 
      gctgattcg ctgtcgcgt gaagataag gaaaaatta 
      ttcaatgaa cacattgaa catttgttg aggaagaag 
      agagagaag ttccgcgag ttattggat gaaaccccc 
      gatgttact ttaaccagt agttggaaa gaaattaaa 
      aaaattata aaagaggat ccccgatat acgaaattt 
      gcgtcaagc gaacggtgt gaacgtgaa tttaaggat 
      tatctcaaa gataaacta ataaccgcc aaaggacaa 
      ttcaaggaa ttgttacag gaaactaaa cttattaca 
      cataaatcg ttatctaat ctcagagag aatcaagga 
      tttatgcaa gaaattgag gatatttta aagaatgat 
      aagagatat cttgtcttg gatcatatt ccgcaagag 
      aggactcag ttgattctg aattatttg gaagaactc 
      gatagaagg ggaccacct ccgccacct acggctagt 
      gaacctaat aggagatct attaaatag ggaattact 
      ttttatcgt atttattat attgtctgg atgctgaag 
      ttgtgcatt ttattgtct tagtggctc cagttgtag 
      ctatttgaa ctgaatgtg ttacacgat taattaaat 
      ttgatttta tactgtaat agaataaaa actccgtat 
      ttac
    • >TC004501 RB
      cataaaccg aagaagtgc atttgttga cgccatttt 
      ccgaaaaat aaatcttaa ttgactcta aatggctaa 
      attgattga aattaaata aaacacaat ggaagaaac 
      agacgtctc catggaata cgacggcgc cgacgtcga 
      cggcgtcgc ccaagagga atacgaaga ggacggagg 
      cttcgaaaa cgacattga actcggaga tgacttcca 
      aatgcgagg ccgaggtcc ttcagggtt caggggccg 
      cggacgcgc gtggccggg tcctccagg tccgccagg 
      accgcgtgg agaaggtcc ccggtttcg aggaagggg 
      atttgggcc cggtggccc tcctccgtt tcgtggacg 
      tggaggacc acctcctcc gatgtttgg acgtggggg 
      acctcccag aaacccacc accagggca gggattcaa 
      tggacctcc gaattttaa tgggcctaa ctggggagg 
      accgatggg gcccccggg gacgatggg tggaccgcc 
      caatatgat gggacccaa tggaccgcc gttcggacc 
      accgggcat gatgggggc gcccccggg tggacccat 
      gggcgctca gggcgggac gaccaatgg gcagtttcc 
      accagtagg gcaaaatcc cacaacagg gttagatgc 
      aaatagtga aatttgggt agagactaa aacctcaga 
      aggcaagtc atattttta taatgctcg tactagaga 
      aaccacttg gactaagcc agaaggtcc taatgtaaa 
      agtaatctc tcaggatca agtggaagc aatggcaca 
      agcggccac cacaggttt agcccaagg aacgtcaac 
      tgcagctca ggcggcgtt agcgcaggc taacgtttc 
      aaataaacc tgaagttga gtcagagga atcgaaagg 
      cgatgccaa acaagctgg gaacgcaat gcctcaaaa 
      catgttagc gggtcctcc gccaggtat gcccccatt 
      tgggggtcc cccaggtca gtttggagc gcctccatt 
      cggcttacc accgccagg attccaagg gacatggcc 
      ggctccagg tcaagcagc atgggcagg ggggccacc 
      aggggctgc tccgtgggg tatgccgcc aggtcttct 
      tacaggttt accaggagc tatcccagc gattgacga 
      agcagcggt tttggctaa agtcgatcc ggaaatcat 
      cgccaaagc gtccgaatg gagcgaaca caaagcacc 
      cgacggacg tttctatta ttacaacgc caagaaagg 
      ggaatcggt ttgggaaaa accgcaagc tcttaagga 
      tttggaaac tgcgaaact ggctgcagc tcaagggat 
      ttcaacaag accgggaac cgaagttat agctgctgt 
      tgagacagg taagccaaa cgctgcagt tgtccaagt 
      ggccaatgg ggacgcagt gaaagacat tgtcataaa 
      ggaggaaga tgacaagat caaaaagcc ccaagacga 
      aacgaaaaa gaagaagga agaagaaga aaaaaagga 
      aactaaaag tcaagataa atcgcgccc agtgtcaag 
      tacccccgt cccaggaac cccatggtg tgttgtgtg 
      gaccggcga tggaagagt tttcttcta caacccatc 
      gtcaaggac atccgtttg ggagcgtcc tgatgagct 
      aataaaacg aacagatgt ggataaaat ggtagcgac 
      acctcccga ttccgtcgg atctcaaac caaaccaga 
      cgctgaaag tccgtcgaa aaaacgcgt atccgacga 
      cagcgacag tgaggaaga aaccccagc taaaaaacc 
      gaaaaaaga agaaataac cagtacgaa cggtaccac 
      tccacaatc agctcgtaa aatcgatat tggcaaaga 
      agcagccat tgaagcgga agtacgtgc ggccaaaga 
      acgtgctat tgtcccact ggaaactcg aattaaatc 
      atttaaaga aatgctggc cgagaaaaa cgtttccgc 
      gtttagtac ttgggaaaa agaactaca taaaattgt 
      tttcgatac acggtatct ccttctaac gtccaagga 
      acgcaaaca agtgtttga aaagtacgt taaagaacg 
      agccgagga agagcgaag agagaaacg gaataagtt 
      gagagagaa gaaggacgc gtttaggaa actgttgag 
      tgagtcgca tctacacgg caaatcgtc gtttagtga 
      cttcgcaca gaagttcgc aaaggacga acgatttaa 
      aggggttga gaaaatgcg cgaacggga aagtctctt 
      caatgaata tttaatcga agtaagaaa gcgcgaaaa 
      agaagaaaa gaatcagcg acgagaaca ggtaaagaa 
      agatttctt tgcaatgct acgcgaaca ctctgatat 
      cgaccgtca ttcccactg ggccgatgt gaaacgaaa 
      agttgattc ggacgctcg atacaaagc cgttgattc 
      gtccggaca acgtgaaga ttggttcag agaatattg 
      caagatact taaagaaga gaaaaagaa agcgaaaga 
      gaaagatcg cgaacacaa acgtgataa agaaaagca 
      taagaagaa ggataaaga taaggataa ggaacgtca 
      ttcgaaaga cgaaaaaga gaaagataa agacaagga 
      gaaagataa atcgaagaa agaagaaga cggagatgt 
      gtcgaataa agaagttga agaaacgga accggaaga 
      aaccgacga cgaaaaagc acaagaaca aagagataa 
      agataggca agccagggc ggaggcgag tctgagaga 
      gagggagaa ggaggtaca aaggactct agcgacgca 
      tatgaggga ccgagataa ggagaggga gcaacacaa 
      acgggacga ggctataca acattttaa tgcgttatt 
      ggccgattt ggtgcgcaa tcccgagct gagctggcg 
      cgaggttaa gcgcatttt gcgaaaaga ccaccgttg 
      ggacttggc tgattcgct gtcgcgtga agataagga 
      aaaattatt caatgaaca cattgaaca tttgttgag 
      gaagaagag agagaagtt ccgcgagtt attggatga 
      aacccccga tgttacttt aaccagtag ttggaaaga 
      aattaaaaa aattataaa agaggatcc ccgatatac 
      gaaatttgc gtcaagcga acggtgtga acgtgaatt 
      taaggatta tctcaaaga taaactaat aaccgccaa 
      aggacaatt caaggaatt gttacagga aactaaact 
      tattacaca taaatcgtt atctaatct cagagagaa 
      tcaaggatt tatgcaaga aattgagga tattttaaa 
      gaatgataa gagatatct tgtcttgga tcatattcc 
      gcaagagag gactcagtt gattctgaa ttatttgga 
      agaactcga tagaagggg accacctcc gccacctac 
      ggctagtga acctaatag gagatctat taaataggg 
      aattacttt ttatcgtat ttattatat tgtctggat 
      gctgaagtt gtgcatttt attgtctta gtggctcca 
      gttgtagct atttgaact gaatgtgtt acacgatta 
      attaaattt gattttata ctgtaatag aataaaaac 
      tccgtattt ac
    • >TC004501 RC
      cataaaccg aagaagtgc atttgttga cgccatttt 
      ccgaaaaat aaatcttaa ttgactcta aatggctaa 
      attgattga aattaaata aaacacaat ggaagaaac 
      agacgtctc catggaata cgacggcgc cgacgtcga 
      cggcgtcgc ccaagagga atacgaaga ggacggagg 
      cttcgaaaa cgacattga actcggaga tgacttcca 
      aatgcgagg ccgaggtcc ttcagggtt caggggccg 
      cggacgcgc gtggccggg tcctccagg tccgccagg 
      accgcgtgg agaaggtcc ccggtttcg aggaagggg 
      atttgggcc cggtggccc tcctccgtt tcgtggacg 
      tggaggacc acctcctcc gatgtttgg acgtggggg 
      acctcccag aaacccacc accagggca gggattcaa 
      tggacctcc gaattttaa tgggcctaa ctggggagg 
      accgatggg gcccccggg gacgatggg tggaccgcc 
      caatatgat gggacccaa tggaccgcc gttcggacc 
      accgggcat gatgggggc gcccccggg tggacccat 
      gggcgctca gggcgggac gaccaatgg gcagtttcc 
      accagtagg gcaaaatcc cacaacagg gttagatgc 
      aaatagtga aatttgggt agagactaa aacctcaga 
      aggcaagtc atattttta taatgctcg tactagaga 
      aaccacttg gactaagcc agaaggtcc taatgtaaa 
      agtaatctc tcaggatca agtggaagc aatggcaca 
      agcggccac cacaggttt agcccaagg aacgtcaac 
      tgcagctca ggcggcgtt agcgcaggc taacgtttc 
      aaataaacc tgaagttga gtcagagga atcgaaagg 
      cgatgccaa acaagctgg gaacgcaat gcctcaaaa 
      catgttagc gggtcctcc gccaggtat gcccccatt 
      tgggggtcc cccaggtca gtttggagc gcctccatt 
      cggcttacc accgccagg attccaagg gacatggcc 
      ggctccagg tcaagcagc atgggcagg ggggccacc 
      aggggctgc tccgtgggg tatgccgcc aggtcttct 
      tacaggttt accaggagc tatcccagc gattgacga 
      agcagcggt tttggctaa agtcgatcc ggaaatcat 
      cgccaaagc gtccgaatg gagcgaaca caaagcacc 
      cgacggacg tttctatta ttacaacgc caagaaagg 
      ggaatcggt ttgggaaaa accgcaagc tcttaagga 
      tttggaaac tgcgaaact ggctgcagc tcaagggat 
      ttcaacaag accgggaac cgaagttat agctgctgt 
      tgagacagg taagccaaa cgctgcagt tgtccaagt 
      ggccaatgg ggacgcagt gaaagacat tgtcataaa 
      ggaggaaga tgacaagat caaaaagcc ccaagacga 
      aacgaaaaa gaagaagga agaagaaga aaaaaagga 
      aactaaaag tcaagataa atcgcgccc agtgtcaag 
      tacccccgt cccaggaac cccatggtg tgttgtgtg 
      gaccggcga tggaagagt tttcttcta caacccatc 
      gtcaaggac atccgtttg ggagcgtcc tgatgagct 
      aataaaacg aacagatgt ggataaaat ggtagcgac 
      acctcccga ttccgtcgg atctcaaac caaaccaga 
      cgctgaaag tccgtcgaa aaaacgcgt atccgacga 
      cagcgacag tgaggaaga aaccccagc taaaaaacc 
      gaaaaaaga agaaataac cagtacgaa cggtaccac 
      tccacaatc agctcgtaa aatcgatat tggcaaaga 
      agcagccat tgaagcgga agtacgtgc ggccaaaga 
      acgtgctat tgtcccact ggaaactcg aattaaatc 
      atttaaaga aatgctggc cgagaaaaa cgtttccgc 
      gtttagtac ttgggaaaa agaactaca taaaattgt 
      tttcgatac acggtatct ccttctaac gtccaagga 
      acgcaaaca agtgtttga aaagtacgt taaagaacg 
      agccgagga agagcgaag agagaaacg gaataagtt 
      gagagagaa gaaggacgc gtttaggaa actgttgag 
      tgagtcgca tctacacgg caaatcgtc gtttagtga 
      cttcgcaca gaagttcgc aaaggacga acgatttaa 
      aggggttga gaaaatgcg cgaacggga aagtctctt 
      caatgaata tttaatcga agtaagaaa gcgcgaaaa 
      agaagaaaa gaatcagcg acgagaaca ggtgagaat 
      gagatgttt tttatccca aacatacga aaaagtaac 
      gatgtaaca agttcctta cttgttaca ttatcggct 
      tgcaatagt agccttttt tagtaagtg ttggtgatc 
      taattttaa ttatttatg ttaatcgat gcgaaccta 
      atcacgcgc acaatacta aaaattatt tacaggttt 
      tcagcactc ggtatgtag atgtgttag attaatttt 
      atttaagga ttgttgaaa attgtgttt ttgccacaa 
      tttttataa cagcgctca ttaatatta taaagcaag 
      agttcatta agtccgatt aaatcaatt ccactttgt 
      agcaactta ctgaactgt tgttaccaa agtttgaca 
      tttgtaacc aaccacaaa ccatttcac ttatttttt 
      agtgcaatt atgtttgtt ttggtttgt tgttggtac 
      ggttgctgt ttcaatgcc tatatttta cgaccaatc 
      aagaacgct aacagattg taaatgatg agtctctta 
      tccagcttc attactttt tcaagagat gtggagtac 
      gggtgaaac acgatttct tccataacc cgaggctag 
      tgtgcatcg agtcagaga agtcgtccc gaggtgtgt 
      gtcagcgct ccttgccgg caatggggc taattttta 
      tttaacggt acttaacct acaagttag ttgttacat 
      ttttaagaa caaaaatca agtaattag taaagattt 
      ttcgtacaa atacaacac aaatcgaaa g
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