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Gene information
General information
TC011194 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19307626..19310006
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011194
      iBeetle screen iB_10669

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011194 RA
        MSEAFTNSY FYALPYEVL AYILSKINT VTLLNFCEV 
        YECDYFLAD RDVTRTIDL CKAYTLPDL VELSKKIVF 
        ENVTTLNVN CLYWVSASK LRRLVRRLI NLETLYAMQ 
        TNLSLTSYD TEVFSKLKI KKMSVSVDR HEGINSHKI 
        PTIETLYLS VIEVTAFIN PWVLHMVFP ALKNLWIDV 
        EKPCDPKII LHCLHAGNK NKIFFQVKA RTPSINFDH 
        YGLSTYYNN RRASQPYVM CCEKFTAKQ LPSESIFEI 
        ERSSEESWR ILDELHCSK PYGPDDARK LFLNESNVD 
        TMFFEELNF YHNGPVCND RCRNAVSQI LKSSCSSKL 
        NKLCIMMCL FENKDAQEA VKTEDSYRA TRERIGIMN 
        KTANHPMEG VFKNFQGLQ HLEVFACNQ TSHHSAIQA 
        YPLITIFEH LECLKVEIP VTLDGSFLI DVLKKCKHL 
        KRLLITSLS QNEKLNMNL ALAIPNATS LKDFRFQNH 
        RIQLDKLLQ RFSLIKTQK LERIVLICE EIDKMNLPS 
        LQEFFDTHP QLIFVYFLI RGSTEAVVR TAQQMLNFY 
        KKKHPSKFF LVKKIDVFN RIRFEIPNV HLCEMVKSD 
        NRVASLNAL DGFVNF
      CDS sequence
      • >TC011194 RA
        atgtctgaa gcgttcaca aactcatat ttttacgca 
        ttaccctac gaagtgctc gcatacatt ttaagcaaa 
        ataaatacg gtaacatta ttaaatttc tgtgaggtg 
        tacgagtgc gactatttt ttggcagac agagatgtt 
        accaggacc atcgatttg tgcaaagct tacacttta 
        ccggacttg gtcgaatta tcgaaaaaa atcgtgttt 
        gagaatgtg actacgcta aatgtgaat tgtttgtat 
        tgggtttcg gcgtcaaaa ttgcgccga ctcgttcgc 
        aggcttatc aatttggaa actttatat gcgatgcag 
        actaatcta agcctcacg tcctacgat acggaagtt 
        tttagcaag ttgaagatt aaaaaaatg agtgtttcg 
        gtggaccga cacgaggga attaacagt cacaaaata 
        ccaactatt gagaccttg tatctcagt gttatcgaa 
        gtgacggct ttcataaac ccctgggtg ctgcatatg 
        gttttcccc gcacttaaa aacttgtgg attgatgtt 
        gagaagcct tgcgacccc aaaatcatc ctccattgc 
        ctccatgct ggcaataaa aataagatt tttttccaa 
        gttaaagcc cgaactccg tccattaac ttcgaccat 
        tacgggttg tccacttat tataataac aggagggcg 
        agtcaacct tacgttatg tgctgtgaa aaattcact 
        gcgaaacaa cttccctcg gaaagtatt ttcgaaatt 
        gagcgcagt tcggaggag agttggcgt attctggac 
        gaattgcat tgttcaaag ccctatgga cctgacgat 
        gcccgaaag ttatttttg aatgaaagc aatgtcgat 
        acgatgttt tttgaagag ctcaatttc taccacaac 
        ggccctgtt tgcaacgat cggtgcaga aacgcagtg 
        tcgcaaatc ttgaagtct tcgtgcagc tccaaactt 
        aacaaactc tgcatcatg atgtgtcta ttcgaaaat 
        aaagatgcc caagaggcg gtgaaaact gaagattcg 
        tacagagcc acccgggag agaatcgga ataatgaat 
        aagactgca aatcatcca atggagggt gtttttaaa 
        aattttcaa gggttgcaa cacttggaa gttttcgcg 
        tgtaatcaa acgtctcat catagtgcc atccaggcg 
        tatcctttg attacgatt tttgaacat ttggagtgc 
        ctcaaagtc gaaattccc gtcactcta gacggaagt 
        tttctaata gatgtttta aaaaaatgt aaacacttg 
        aagcgcctc ttgatcacg tcgttgtcg caaaacgaa 
        aaattaaat atgaatcta gccttggct attccgaat 
        gcaacatct ttaaaagac ttcagattt cagaaccac 
        cgaatccag ttggacaaa ctcctccag cgtttctct 
        ctcattaaa acacaaaaa ctggagcga attgttcta 
        atttgtgag gagattgat aaaatgaat ctaccctct 
        ctgcaggaa ttcttcgac acccacccc caacttata 
        ttcgtgtat ttcctgatc cggggaagt acagaagcg 
        gtggttcga acggcccaa caaatgtta aatttttac 
        aaaaagaaa cacccctcg aaatttttt ctcgtcaag 
        aaaattgat gttttcaac cgaattcgt ttcgagata 
        cctaatgtt catttatgt gaaatggtg aaaagcgac 
        aaccgcgtt gccagtctg aatgcctta gacggtttt 
        gtgaatttt taa
      mRNA sequence
      • >TC011194 RA
        cctcaaact ttcacactt tttatccgg tattatttt 
        atttatttg ttaattacg tattacata tcattaata 
        ttatgtctg aagcgttca caaactcat atttttacg 
        cattaccct acgaagtgc tcgcataca ttttaagca 
        aaataaata cggtaacat tattaaatt tctgtgagg 
        tgtacgagt gcgactatt ttttggcag acagagatg 
        ttaccagga ccatcgatt tgtgcaaag cttacactt 
        taccggact tggtcgaat tatcgaaaa aaatcgtgt 
        ttgagaatg tgactacgc taaatgtga attgtttgt 
        attgggttt cggcgtcaa aattgcgcc gactcgttc 
        gcaggctta tcaatttgg aaactttat atgcgatgc 
        agactaatc taagcctca cgtcctacg atacggaag 
        tttttagca agttgaaga ttaaaaaaa tgagtgttt 
        cggtggacc gacacgagg gaattaaca gtcacaaaa 
        taccaacta ttgagacct tgtatctca gtgttatcg 
        aagtgacgg ctttcataa acccctggg tgctgcata 
        tggttttcc ccgcactta aaaacttgt ggattgatg 
        ttgagaagc cttgcgacc ccaaaatca tcctccatt 
        gcctccatg ctggcaata aaaataaga tttttttcc 
        aagttaaag cccgaactc cgtccatta acttcgacc 
        attacgggt tgtccactt attataata acaggaggg 
        cgagtcaac cttacgtta tgtgctgtg aaaaattca 
        ctgcgaaac aacttccct cggaaagta ttttcgaaa 
        ttgagcgca gttcggagg agagttggc gtattctgg 
        acgaattgc attgttcaa agccctatg gacctgacg 
        atgcccgaa agttatttt tgaatgaaa gcaatgtcg 
        atacgatgt tttttgaag agctcaatt tctaccaca 
        acggccctg tttgcaacg atcggtgca gaaacgcag 
        tgtcgcaaa tcttgaagt cttcgtgca gctccaaac 
        ttaacaaac tctgcatca tgatgtgtc tattcgaaa 
        ataaagatg cccaagagg cggtgaaaa ctgaagatt 
        cgtacagag ccacccggg agagaatcg gaataatga 
        ataagactg caaatcatc caatggagg gtgttttta 
        aaaattttc aagggttgc aacacttgg aagttttcg 
        cgtgtaatc aaacgtctc atcatagtg ccatccagg 
        cgtatcctt tgattacga tttttgaac atttggagt 
        gcctcaaag tcgaaattc ccgtcactc tagacggaa 
        gttttctaa tagatgttt taaaaaaat gtaaacact 
        tgaagcgcc tcttgatca cgtcgttgt cgcaaaacg 
        aaaaattaa atatgaatc tagccttgg ctattccga 
        atgcaacat ctttaaaag acttcagat ttcagaacc 
        accgaatcc agttggaca aactcctcc agcgtttct 
        ctctcatta aaacacaaa aactggagc gaattgttc 
        taatttgtg aggagattg ataaaatga atctaccct 
        ctctgcagg aattcttcg acacccacc cccaactta 
        tattcgtgt atttcctga tccggggaa gtacagaag 
        cggtggttc gaacggccc aacaaatgt taaattttt 
        acaaaaaga aacacccct cgaaatttt ttctcgtca 
        agaaaattg atgttttca accgaattc gtttcgaga 
        tacctaatg ttcatttat gtgaaatgg tgaaaagcg 
        acaaccgcg ttgccagtc tgaatgcct tagacggtt 
        ttgtgaatt tttaactta ttttgttga ttgtagttg 
        ttacaaaaa taaaataaa taaataaaa tcaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY