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Gene information
General information
TC012622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12220604..12223625
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012622
    iBeetle screen iB_10668

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012622 RA
      MSSLKLLSF LKLAPQCLR HYSETSFLT GTSSQYIED 
MYNAWLKDP SSVHVSWDS FFRNTSQGL PQPYHAPPN 
LAPSKGALV SQIAAVATP PQTAPLDER TIEDHLAVQ 
AVIRSYQAR GHLVAKLDP LEIMFNDKT TTTVSQTGS 
PPEEILRTF RLENLDKVY KLPSSTYIG AEGEKKLPL 
REILHRLEL AYCRHIGVE YMYIDDLEQ RDFIRRRME 
APGVLNQST VQKKLTLTR LIRTVEFEN FLAKKWASE 
KRFGIEGCD MFIPGLEQI IDKSTEHGV EHFFLGLSH 
RGRLNTLAN ILRKPLYQI FNQFVPLEP ADLGSGDVK 
YHHGSHTQR TNQLTQKHY KVTLLANPS HLESVNSVV 
LGRTRAEQF YKGDDEGKK SLAILIHGD AAFSGQGVN 
YESYGLSYL PHYTTHGAI CFIINNQVG FTTDPRFSR 
SSRYCSDLG KVVNAPIFH VNADDPESV IHVCNIAAE 
WRAKFHKDI IIDLVGYRR HGHNEADEP MFTQPLMYT 
KIKSMASIG DKYSSELLK EKVVTKDEI KHVKDDYNK 
LCEDEYVKA SKQTQIFIR DWLDSPWSG FFEGKDPLK 
VTPTGVPEA TLAHIGAKV SSPPPPNLD FVLHKGITR 
ILNQREAMV KNRQIDWAL GEAMAIGSL VKEGVHVRL 
SGEDVERGT FSHRHHVYH HQTVDKMTY QPLGDVFDA 
QAPYVVCNS SLSEYGVLG FEVGYALAN PNSLILWEA 
QFGDFYNTA QCIMDQFLC CGQAKWMRQ IGLVVLLPH 
GFDGMGPEH SSGRPERFL QNCDDDPDV VPPPGPDFA 
IKQLHDCNW IVANCSTPA NLFHIWRRQ VALPFRKPL 
ILFSPKNLL RHPECKSSF DEMLEGTEF KRVIPDSGP 
ASQDPQNVK KLILCTGKV YYDIMDAFK KKKVGKEIA 
VTRVEQLSP FPYDLLKTE FEKYPNAKI CWAQEEHKN 
GGPYLYVLA RLNTLLNRS REIHYIGRA VSAAPATGT 
KAVHLKEVE MLANEFVAL
    CDS sequence
    • >TC012622 RA
      atgtcaagt ttaaaactc ctctccttc ttaaaatta 
gccccacaa tgcctccgc cactactcg gaaacctcc 
ttcctcact ggaacctca tctcaatac atcgaagac 
atgtacaat gcttggctc aaagaccca tccagcgtc 
cacgtctcc tgggactcc ttcttccgc aacaccagc 
caaggcctc ccccaaccc taccacgcc cctccgaac 
ctggcgccc tccaaaggc gccctagtc tcccaaatc 
gcggccgtc gcaaccccc ccacaaacc gcccccctc 
gacgaaaga acaatcgag gaccattta gccgtccag 
gccgtcatt cgcagctac caagcccgc ggccatcta 
gtcgccaaa ctagaccct ttggaaatc atgttcaat 
gataaaacc acgaccacg gtgtcgcaa accgggtca 
ccaccggag gaaatcctc cgaacattc cgcctggaa 
aacctggac aaagtctac aaactgccg tcgagcacg 
tacatcggc gccgagggc gaaaagaaa ctccctctc 
cgtgagatt ttgcaccgg ctggagttg gcttactgc 
cgccacatc ggggtggag tacatgtac atcgatgat 
ttggaacag agggatttt atccggcgc cggatggaa 
gccccaggt gtgcttaac cagagcacg gtccagaaa 
aaactcacc ttgactagg cttattaga acggtcgag 
tttgagaat ttcttggcc aagaaatgg gcctccgag 
aagcggttc gggatcgaa ggctgcgac atgttcatt 
cctggactt gaacaaatc atcgacaag tcgacggaa 
cacggcgtc gagcatttt ttcctcggg ttgagtcac 
aggggccgt ttgaacacc ctggctaat attcttcgg 
aagccgctc taccagatt ttcaaccaa tttgtgccg 
ctggagccc gcggatttg gggtccggc gacgtgaag 
taccaccac gggagtcac acccagcgc accaaccag 
ctcacccag aagcactac aaagtcacg ctgctggcg 
aacccatcg cacttggag agcgtcaac tcggtggtg 
cttgggcgc acccgcgcc gagcagttc tacaaaggc 
gacgatgag ggcaagaag agtttggcg attttgatc 
cacggagac gccgctttt tctggccaa ggggtcaac 
tacgagagc tacggtctg tcctacttg ccccactac 
accacacac ggggctatc tgtttcatt ataaacaac 
caggtgggg ttcaccacc gatcctcgg ttttcccgg 
tcgtcgcgc tactgctcc gacttgggc aaagtcgtg 
aacgcgccc attttccac gtgaacgcc gacgacccc 
gagagcgtt atccacgtg tgcaacatc gcggcggag 
tggagggcc aagttccac aaagacatc atcatcgac 
ttggtcggg taccgccgc cacggccac aacgaagcc 
gacgagccc atgttcacc cagcccctg atgtacacc 
aagatcaag agcatggcc tccatcggg gacaagtac 
tccagcgag ttgctgaag gagaaagtg gtgacgaag 
gacgagatc aagcacgtg aaggacgat tacaacaaa 
ctgtgcgaa gacgagtac gtgaaggcg tccaaacaa 
acacaaatc ttcatcaga gattggttg gactcgcct 
tggtcgggc ttcttcgag gggaaagac ccgctcaag 
gtgaccccc accggggtg ccggaagcc accctcgcg 
cacatcggg gccaaagtc tcctcgccg ccgccccca 
aacctcgac ttcgtgctc cacaagggc ataactcgc 
attctgaac caacgcgaa gcgatggtc aagaaccgg 
caaatcgac tgggctttg ggggaggcg atggccatc 
gggtccctc gtcaaggag ggcgtccac gtgcgcctc 
tccggcgag gacgtcgag cgcgggacg ttctcgcac 
cgccaccac gtctaccac caccagacc gtggacaaa 
atgacctac cagcccctc ggtgacgtc ttcgacgcc 
caagcgccc tacgtggtg tgcaacagc tcgctgtcc 
gagtacggc gtcctgggc ttcgaggtg ggctatgcc 
ctggccaac cccaactcc ctgatcctg tgggaagcc 
cagttcgga gatttctac aacacggcg cagtgcata 
atggaccag tttctgtgc tgtggccag gccaagtgg 
atgcggcag atcggcctc gttgtgctc ctgccgcat 
gggttcgac ggcatgggc ccggagcac tccagcggc 
cggcctgag cgcttcctg cagaactgt gacgacgac 
cccgacgtg gtcccgccc ccaggaccc gactttgct 
attaaacaa ctccacgat tgcaactgg atcgtggcc 
aattgttcc acacccgcc aacctcttc cacatctgg 
cggcgccaa gtcgctcta ccattccgc aaacccttg 
atcctgttc agtcccaaa aacctgcta aggcacccc 
gagtgcaaa agctccttc gacgaaatg ctcgaaggg 
acggaattc aaacgagtt atccccgac agtgggcca 
gcgagtcag gacccgcaa aacgtcaag aaactcatc 
ctttgcacc gggaaggtg tattacgat attatggac 
gctttcaaa aagaaaaaa gtcgggaag gagatcgcc 
gttacgaga gtcgagcaa ttgtcgccg tttccgtac 
gatttgctt aaaaccgaa ttcgagaag tatccaaac 
gcgaaaatt tgctgggct caagaagag cacaagaac 
ggggggccg tatttgtac gttttggcg cggttgaac 
acacttttg aataggtca cgcgaaatt cattatatt 
gggagggcg gtgtccgca gccccggcc acgggaacc 
aaagcagtg catctaaag gaagtcgaa atgttggcg 
aacgaattt gtggcgctt tag
    mRNA sequence
    • >TC012622 RA
      cccttctaa atcatgtca agtttaaaa ctcctctcc 
ttcttaaaa ttagcccca caatgcctc cgccactac 
tcggaaacc tccttcctc actggaacc tcatctcaa 
tacatcgaa gacatgtac aatgcttgg ctcaaagac 
ccatccagc gtccacgtc tcctgggac tccttcttc 
cgcaacacc agccaaggc ctcccccaa ccctaccac 
gcccctccg aacctggcg ccctccaaa ggcgcccta 
gtctcccaa atcgcggcc gtcgcaacc cccccacaa 
accgccccc ctcgacgaa agaacaatc gaggaccat 
ttagccgtc caggccgtc attcgcagc taccaagcc 
cgcggccat ctagtcgcc aaactagac cctttggaa 
atcatgttc aatgataaa accacgacc acggtgtcg 
caaaccggg tcaccaccg gaggaaatc ctccgaaca 
ttccgcctg gaaaacctg gacaaagtc tacaaactg 
ccgtcgagc acgtacatc ggcgccgag ggcgaaaag 
aaactccct ctccgtgag attttgcac cggctggag 
ttggcttac tgccgccac atcggggtg gagtacatg 
tacatcgat gatttggaa cagagggat tttatccgg 
cgccggatg gaagcccca ggtgtgctt aaccagagc 
acggtccag aaaaaactc accttgact aggcttatt 
agaacggtc gagtttgag aatttcttg gccaagaaa 
tgggcctcc gagaagcgg ttcgggatc gaaggctgc 
gacatgttc attcctgga cttgaacaa atcatcgac 
aagtcgacg gaacacggc gtcgagcat tttttcctc 
gggttgagt cacaggggc cgtttgaac accctggct 
aatattctt cggaagccg ctctaccag attttcaac 
caatttgtg ccgctggag cccgcggat ttggggtcc 
ggcgacgtg aagtaccac cacgggagt cacacccag 
cgcaccaac cagctcacc cagaagcac tacaaagtc 
acgctgctg gcgaaccca tcgcacttg gagagcgtc 
aactcggtg gtgcttggg cgcacccgc gccgagcag 
ttctacaaa ggcgacgat gagggcaag aagagtttg 
gcgattttg atccacgga gacgccgct ttttctggc 
caaggggtc aactacgag agctacggt ctgtcctac 
ttgccccac tacaccaca cacggggct atctgtttc 
attataaac aaccaggtg gggttcacc accgatcct 
cggttttcc cggtcgtcg cgctactgc tccgacttg 
ggcaaagtc gtgaacgcg cccattttc cacgtgaac 
gccgacgac cccgagagc gttatccac gtgtgcaac 
atcgcggcg gagtggagg gccaagttc cacaaagac 
atcatcatc gacttggtc gggtaccgc cgccacggc 
cacaacgaa gccgacgag cccatgttc acccagccc 
ctgatgtac accaagatc aagagcatg gcctccatc 
ggggacaag tactccagc gagttgctg aaggagaaa 
gtggtgacg aaggacgag atcaagcac gtgaaggac 
gattacaac aaactgtgc gaagacgag tacgtgaag 
gcgtccaaa caaacacaa atcttcatc agagattgg 
ttggactcg ccttggtcg ggcttcttc gaggggaaa 
gacccgctc aaggtgacc cccaccggg gtgccggaa 
gccaccctc gcgcacatc ggggccaaa gtctcctcg 
ccgccgccc ccaaacctc gacttcgtg ctccacaag 
ggcataact cgcattctg aaccaacgc gaagcgatg 
gtcaagaac cggcaaatc gactgggct ttgggggag 
gcgatggcc atcgggtcc ctcgtcaag gagggcgtc 
cacgtgcgc ctctccggc gaggacgtc gagcgcggg 
acgttctcg caccgccac cacgtctac caccaccag 
accgtggac aaaatgacc taccagccc ctcggtgac 
gtcttcgac gcccaagcg ccctacgtg gtgtgcaac 
agctcgctg tccgagtac ggcgtcctg ggcttcgag 
gtgggctat gccctggcc aaccccaac tccctgatc 
ctgtgggaa gcccagttc ggagatttc tacaacacg 
gcgcagtgc ataatggac cagtttctg tgctgtggc 
caggccaag tggatgcgg cagatcggc ctcgttgtg 
ctcctgccg catgggttc gacggcatg ggcccggag 
cactccagc ggccggcct gagcgcttc ctgcagaac 
tgtgacgac gaccccgac gtggtcccg cccccagga 
cccgacttt gctattaaa caactccac gattgcaac 
tggatcgtg gccaattgt tccacaccc gccaacctc 
ttccacatc tggcggcgc caagtcgct ctaccattc 
cgcaaaccc ttgatcctg ttcagtccc aaaaacctg 
ctaaggcac cccgagtgc aaaagctcc ttcgacgaa 
atgctcgaa gggacggaa ttcaaacga gttatcccc 
gacagtggg ccagcgagt caggacccg caaaacgtc 
aagaaactc atcctttgc accgggaag gtgtattac 
gatattatg gacgctttc aaaaagaaa aaagtcggg 
aaggagatc gccgttacg agagtcgag caattgtcg 
ccgtttccg tacgatttg cttaaaacc gaattcgag 
aagtatcca aacgcgaaa atttgctgg gctcaagaa 
gagcacaag aacgggggg ccgtatttg tacgttttg 
gcgcggttg aacacactt ttgaatagg tcacgcgaa 
attcattat attgggagg gcggtgtcc gcagccccg 
gccacggga accaaagca gtgcatcta aaggaagtc 
gaaatgttg gcgaacgaa tttgtggcg ctttagtac 
tgtatctcg taaaaaata aaagtttcg tcattgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY