| General information | |||
| TC012622 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 12220604..12223625 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012622 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10668 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012622 RA
MSSLKLLSF LKLAPQCLR HYSETSFLT GTSSQYIED MYNAWLKDP SSVHVSWDS FFRNTSQGL PQPYHAPPN LAPSKGALV SQIAAVATP PQTAPLDER TIEDHLAVQ AVIRSYQAR GHLVAKLDP LEIMFNDKT TTTVSQTGS PPEEILRTF RLENLDKVY KLPSSTYIG AEGEKKLPL REILHRLEL AYCRHIGVE YMYIDDLEQ RDFIRRRME APGVLNQST VQKKLTLTR LIRTVEFEN FLAKKWASE KRFGIEGCD MFIPGLEQI IDKSTEHGV EHFFLGLSH RGRLNTLAN ILRKPLYQI FNQFVPLEP ADLGSGDVK YHHGSHTQR TNQLTQKHY KVTLLANPS HLESVNSVV LGRTRAEQF YKGDDEGKK SLAILIHGD AAFSGQGVN YESYGLSYL PHYTTHGAI CFIINNQVG FTTDPRFSR SSRYCSDLG KVVNAPIFH VNADDPESV IHVCNIAAE WRAKFHKDI IIDLVGYRR HGHNEADEP MFTQPLMYT KIKSMASIG DKYSSELLK EKVVTKDEI KHVKDDYNK LCEDEYVKA SKQTQIFIR DWLDSPWSG FFEGKDPLK VTPTGVPEA TLAHIGAKV SSPPPPNLD FVLHKGITR ILNQREAMV KNRQIDWAL GEAMAIGSL VKEGVHVRL SGEDVERGT FSHRHHVYH HQTVDKMTY QPLGDVFDA QAPYVVCNS SLSEYGVLG FEVGYALAN PNSLILWEA QFGDFYNTA QCIMDQFLC CGQAKWMRQ IGLVVLLPH GFDGMGPEH SSGRPERFL QNCDDDPDV VPPPGPDFA IKQLHDCNW IVANCSTPA NLFHIWRRQ VALPFRKPL ILFSPKNLL RHPECKSSF DEMLEGTEF KRVIPDSGP ASQDPQNVK KLILCTGKV YYDIMDAFK KKKVGKEIA VTRVEQLSP FPYDLLKTE FEKYPNAKI CWAQEEHKN GGPYLYVLA RLNTLLNRS REIHYIGRA VSAAPATGT KAVHLKEVE MLANEFVAL
- >TC012622 RA
atgtcaagt ttaaaactc ctctccttc ttaaaatta gccccacaa tgcctccgc cactactcg gaaacctcc ttcctcact ggaacctca tctcaatac atcgaagac atgtacaat gcttggctc aaagaccca tccagcgtc cacgtctcc tgggactcc ttcttccgc aacaccagc caaggcctc ccccaaccc taccacgcc cctccgaac ctggcgccc tccaaaggc gccctagtc tcccaaatc gcggccgtc gcaaccccc ccacaaacc gcccccctc gacgaaaga acaatcgag gaccattta gccgtccag gccgtcatt cgcagctac caagcccgc ggccatcta gtcgccaaa ctagaccct ttggaaatc atgttcaat gataaaacc acgaccacg gtgtcgcaa accgggtca ccaccggag gaaatcctc cgaacattc cgcctggaa aacctggac aaagtctac aaactgccg tcgagcacg tacatcggc gccgagggc gaaaagaaa ctccctctc cgtgagatt ttgcaccgg ctggagttg gcttactgc cgccacatc ggggtggag tacatgtac atcgatgat ttggaacag agggatttt atccggcgc cggatggaa gccccaggt gtgcttaac cagagcacg gtccagaaa aaactcacc ttgactagg cttattaga acggtcgag tttgagaat ttcttggcc aagaaatgg gcctccgag aagcggttc gggatcgaa ggctgcgac atgttcatt cctggactt gaacaaatc atcgacaag tcgacggaa cacggcgtc gagcatttt ttcctcggg ttgagtcac aggggccgt ttgaacacc ctggctaat attcttcgg aagccgctc taccagatt ttcaaccaa tttgtgccg ctggagccc gcggatttg gggtccggc gacgtgaag taccaccac gggagtcac acccagcgc accaaccag ctcacccag aagcactac aaagtcacg ctgctggcg aacccatcg cacttggag agcgtcaac tcggtggtg cttgggcgc acccgcgcc gagcagttc tacaaaggc gacgatgag ggcaagaag agtttggcg attttgatc cacggagac gccgctttt tctggccaa ggggtcaac tacgagagc tacggtctg tcctacttg ccccactac accacacac ggggctatc tgtttcatt ataaacaac caggtgggg ttcaccacc gatcctcgg ttttcccgg tcgtcgcgc tactgctcc gacttgggc aaagtcgtg aacgcgccc attttccac gtgaacgcc gacgacccc gagagcgtt atccacgtg tgcaacatc gcggcggag tggagggcc aagttccac aaagacatc atcatcgac ttggtcggg taccgccgc cacggccac aacgaagcc gacgagccc atgttcacc cagcccctg atgtacacc aagatcaag agcatggcc tccatcggg gacaagtac tccagcgag ttgctgaag gagaaagtg gtgacgaag gacgagatc aagcacgtg aaggacgat tacaacaaa ctgtgcgaa gacgagtac gtgaaggcg tccaaacaa acacaaatc ttcatcaga gattggttg gactcgcct tggtcgggc ttcttcgag gggaaagac ccgctcaag gtgaccccc accggggtg ccggaagcc accctcgcg cacatcggg gccaaagtc tcctcgccg ccgccccca aacctcgac ttcgtgctc cacaagggc ataactcgc attctgaac caacgcgaa gcgatggtc aagaaccgg caaatcgac tgggctttg ggggaggcg atggccatc gggtccctc gtcaaggag ggcgtccac gtgcgcctc tccggcgag gacgtcgag cgcgggacg ttctcgcac cgccaccac gtctaccac caccagacc gtggacaaa atgacctac cagcccctc ggtgacgtc ttcgacgcc caagcgccc tacgtggtg tgcaacagc tcgctgtcc gagtacggc gtcctgggc ttcgaggtg ggctatgcc ctggccaac cccaactcc ctgatcctg tgggaagcc cagttcgga gatttctac aacacggcg cagtgcata atggaccag tttctgtgc tgtggccag gccaagtgg atgcggcag atcggcctc gttgtgctc ctgccgcat gggttcgac ggcatgggc ccggagcac tccagcggc cggcctgag cgcttcctg cagaactgt gacgacgac cccgacgtg gtcccgccc ccaggaccc gactttgct attaaacaa ctccacgat tgcaactgg atcgtggcc aattgttcc acacccgcc aacctcttc cacatctgg cggcgccaa gtcgctcta ccattccgc aaacccttg atcctgttc agtcccaaa aacctgcta aggcacccc gagtgcaaa agctccttc gacgaaatg ctcgaaggg acggaattc aaacgagtt atccccgac agtgggcca gcgagtcag gacccgcaa aacgtcaag aaactcatc ctttgcacc gggaaggtg tattacgat attatggac gctttcaaa aagaaaaaa gtcgggaag gagatcgcc gttacgaga gtcgagcaa ttgtcgccg tttccgtac gatttgctt aaaaccgaa ttcgagaag tatccaaac gcgaaaatt tgctgggct caagaagag cacaagaac ggggggccg tatttgtac gttttggcg cggttgaac acacttttg aataggtca cgcgaaatt cattatatt gggagggcg gtgtccgca gccccggcc acgggaacc aaagcagtg catctaaag gaagtcgaa atgttggcg aacgaattt gtggcgctt tag
- >TC012622 RA
cccttctaa atcatgtca agtttaaaa ctcctctcc ttcttaaaa ttagcccca caatgcctc cgccactac tcggaaacc tccttcctc actggaacc tcatctcaa tacatcgaa gacatgtac aatgcttgg ctcaaagac ccatccagc gtccacgtc tcctgggac tccttcttc cgcaacacc agccaaggc ctcccccaa ccctaccac gcccctccg aacctggcg ccctccaaa ggcgcccta gtctcccaa atcgcggcc gtcgcaacc cccccacaa accgccccc ctcgacgaa agaacaatc gaggaccat ttagccgtc caggccgtc attcgcagc taccaagcc cgcggccat ctagtcgcc aaactagac cctttggaa atcatgttc aatgataaa accacgacc acggtgtcg caaaccggg tcaccaccg gaggaaatc ctccgaaca ttccgcctg gaaaacctg gacaaagtc tacaaactg ccgtcgagc acgtacatc ggcgccgag ggcgaaaag aaactccct ctccgtgag attttgcac cggctggag ttggcttac tgccgccac atcggggtg gagtacatg tacatcgat gatttggaa cagagggat tttatccgg cgccggatg gaagcccca ggtgtgctt aaccagagc acggtccag aaaaaactc accttgact aggcttatt agaacggtc gagtttgag aatttcttg gccaagaaa tgggcctcc gagaagcgg ttcgggatc gaaggctgc gacatgttc attcctgga cttgaacaa atcatcgac aagtcgacg gaacacggc gtcgagcat tttttcctc gggttgagt cacaggggc cgtttgaac accctggct aatattctt cggaagccg ctctaccag attttcaac caatttgtg ccgctggag cccgcggat ttggggtcc ggcgacgtg aagtaccac cacgggagt cacacccag cgcaccaac cagctcacc cagaagcac tacaaagtc acgctgctg gcgaaccca tcgcacttg gagagcgtc aactcggtg gtgcttggg cgcacccgc gccgagcag ttctacaaa ggcgacgat gagggcaag aagagtttg gcgattttg atccacgga gacgccgct ttttctggc caaggggtc aactacgag agctacggt ctgtcctac ttgccccac tacaccaca cacggggct atctgtttc attataaac aaccaggtg gggttcacc accgatcct cggttttcc cggtcgtcg cgctactgc tccgacttg ggcaaagtc gtgaacgcg cccattttc cacgtgaac gccgacgac cccgagagc gttatccac gtgtgcaac atcgcggcg gagtggagg gccaagttc cacaaagac atcatcatc gacttggtc gggtaccgc cgccacggc cacaacgaa gccgacgag cccatgttc acccagccc ctgatgtac accaagatc aagagcatg gcctccatc ggggacaag tactccagc gagttgctg aaggagaaa gtggtgacg aaggacgag atcaagcac gtgaaggac gattacaac aaactgtgc gaagacgag tacgtgaag gcgtccaaa caaacacaa atcttcatc agagattgg ttggactcg ccttggtcg ggcttcttc gaggggaaa gacccgctc aaggtgacc cccaccggg gtgccggaa gccaccctc gcgcacatc ggggccaaa gtctcctcg ccgccgccc ccaaacctc gacttcgtg ctccacaag ggcataact cgcattctg aaccaacgc gaagcgatg gtcaagaac cggcaaatc gactgggct ttgggggag gcgatggcc atcgggtcc ctcgtcaag gagggcgtc cacgtgcgc ctctccggc gaggacgtc gagcgcggg acgttctcg caccgccac cacgtctac caccaccag accgtggac aaaatgacc taccagccc ctcggtgac gtcttcgac gcccaagcg ccctacgtg gtgtgcaac agctcgctg tccgagtac ggcgtcctg ggcttcgag gtgggctat gccctggcc aaccccaac tccctgatc ctgtgggaa gcccagttc ggagatttc tacaacacg gcgcagtgc ataatggac cagtttctg tgctgtggc caggccaag tggatgcgg cagatcggc ctcgttgtg ctcctgccg catgggttc gacggcatg ggcccggag cactccagc ggccggcct gagcgcttc ctgcagaac tgtgacgac gaccccgac gtggtcccg cccccagga cccgacttt gctattaaa caactccac gattgcaac tggatcgtg gccaattgt tccacaccc gccaacctc ttccacatc tggcggcgc caagtcgct ctaccattc cgcaaaccc ttgatcctg ttcagtccc aaaaacctg ctaaggcac cccgagtgc aaaagctcc ttcgacgaa atgctcgaa gggacggaa ttcaaacga gttatcccc gacagtggg ccagcgagt caggacccg caaaacgtc aagaaactc atcctttgc accgggaag gtgtattac gatattatg gacgctttc aaaaagaaa aaagtcggg aaggagatc gccgttacg agagtcgag caattgtcg ccgtttccg tacgatttg cttaaaacc gaattcgag aagtatcca aacgcgaaa atttgctgg gctcaagaa gagcacaag aacgggggg ccgtatttg tacgttttg gcgcggttg aacacactt ttgaatagg tcacgcgaa attcattat attgggagg gcggtgtcc gcagccccg gccacggga accaaagca gtgcatcta aaggaagtc gaaatgttg gcgaacgaa tttgtggcg ctttagtac tgtatctcg taaaaaata aaagtttcg tcattgc
