Status
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Gene information
General information
TC012622 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 12220604..12223625
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012622
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10668

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012622 RA
      MSSLKLLSF LKLAPQCLR HYSETSFLT GTSSQYIED 
      MYNAWLKDP SSVHVSWDS FFRNTSQGL PQPYHAPPN 
      LAPSKGALV SQIAAVATP PQTAPLDER TIEDHLAVQ 
      AVIRSYQAR GHLVAKLDP LEIMFNDKT TTTVSQTGS 
      PPEEILRTF RLENLDKVY KLPSSTYIG AEGEKKLPL 
      REILHRLEL AYCRHIGVE YMYIDDLEQ RDFIRRRME 
      APGVLNQST VQKKLTLTR LIRTVEFEN FLAKKWASE 
      KRFGIEGCD MFIPGLEQI IDKSTEHGV EHFFLGLSH 
      RGRLNTLAN ILRKPLYQI FNQFVPLEP ADLGSGDVK 
      YHHGSHTQR TNQLTQKHY KVTLLANPS HLESVNSVV 
      LGRTRAEQF YKGDDEGKK SLAILIHGD AAFSGQGVN 
      YESYGLSYL PHYTTHGAI CFIINNQVG FTTDPRFSR 
      SSRYCSDLG KVVNAPIFH VNADDPESV IHVCNIAAE 
      WRAKFHKDI IIDLVGYRR HGHNEADEP MFTQPLMYT 
      KIKSMASIG DKYSSELLK EKVVTKDEI KHVKDDYNK 
      LCEDEYVKA SKQTQIFIR DWLDSPWSG FFEGKDPLK 
      VTPTGVPEA TLAHIGAKV SSPPPPNLD FVLHKGITR 
      ILNQREAMV KNRQIDWAL GEAMAIGSL VKEGVHVRL 
      SGEDVERGT FSHRHHVYH HQTVDKMTY QPLGDVFDA 
      QAPYVVCNS SLSEYGVLG FEVGYALAN PNSLILWEA 
      QFGDFYNTA QCIMDQFLC CGQAKWMRQ IGLVVLLPH 
      GFDGMGPEH SSGRPERFL QNCDDDPDV VPPPGPDFA 
      IKQLHDCNW IVANCSTPA NLFHIWRRQ VALPFRKPL 
      ILFSPKNLL RHPECKSSF DEMLEGTEF KRVIPDSGP 
      ASQDPQNVK KLILCTGKV YYDIMDAFK KKKVGKEIA 
      VTRVEQLSP FPYDLLKTE FEKYPNAKI CWAQEEHKN 
      GGPYLYVLA RLNTLLNRS REIHYIGRA VSAAPATGT 
      KAVHLKEVE MLANEFVAL
    CDS sequence
    • >TC012622 RA
      atgtcaagt ttaaaactc ctctccttc ttaaaatta 
      gccccacaa tgcctccgc cactactcg gaaacctcc 
      ttcctcact ggaacctca tctcaatac atcgaagac 
      atgtacaat gcttggctc aaagaccca tccagcgtc 
      cacgtctcc tgggactcc ttcttccgc aacaccagc 
      caaggcctc ccccaaccc taccacgcc cctccgaac 
      ctggcgccc tccaaaggc gccctagtc tcccaaatc 
      gcggccgtc gcaaccccc ccacaaacc gcccccctc 
      gacgaaaga acaatcgag gaccattta gccgtccag 
      gccgtcatt cgcagctac caagcccgc ggccatcta 
      gtcgccaaa ctagaccct ttggaaatc atgttcaat 
      gataaaacc acgaccacg gtgtcgcaa accgggtca 
      ccaccggag gaaatcctc cgaacattc cgcctggaa 
      aacctggac aaagtctac aaactgccg tcgagcacg 
      tacatcggc gccgagggc gaaaagaaa ctccctctc 
      cgtgagatt ttgcaccgg ctggagttg gcttactgc 
      cgccacatc ggggtggag tacatgtac atcgatgat 
      ttggaacag agggatttt atccggcgc cggatggaa 
      gccccaggt gtgcttaac cagagcacg gtccagaaa 
      aaactcacc ttgactagg cttattaga acggtcgag 
      tttgagaat ttcttggcc aagaaatgg gcctccgag 
      aagcggttc gggatcgaa ggctgcgac atgttcatt 
      cctggactt gaacaaatc atcgacaag tcgacggaa 
      cacggcgtc gagcatttt ttcctcggg ttgagtcac 
      aggggccgt ttgaacacc ctggctaat attcttcgg 
      aagccgctc taccagatt ttcaaccaa tttgtgccg 
      ctggagccc gcggatttg gggtccggc gacgtgaag 
      taccaccac gggagtcac acccagcgc accaaccag 
      ctcacccag aagcactac aaagtcacg ctgctggcg 
      aacccatcg cacttggag agcgtcaac tcggtggtg 
      cttgggcgc acccgcgcc gagcagttc tacaaaggc 
      gacgatgag ggcaagaag agtttggcg attttgatc 
      cacggagac gccgctttt tctggccaa ggggtcaac 
      tacgagagc tacggtctg tcctacttg ccccactac 
      accacacac ggggctatc tgtttcatt ataaacaac 
      caggtgggg ttcaccacc gatcctcgg ttttcccgg 
      tcgtcgcgc tactgctcc gacttgggc aaagtcgtg 
      aacgcgccc attttccac gtgaacgcc gacgacccc 
      gagagcgtt atccacgtg tgcaacatc gcggcggag 
      tggagggcc aagttccac aaagacatc atcatcgac 
      ttggtcggg taccgccgc cacggccac aacgaagcc 
      gacgagccc atgttcacc cagcccctg atgtacacc 
      aagatcaag agcatggcc tccatcggg gacaagtac 
      tccagcgag ttgctgaag gagaaagtg gtgacgaag 
      gacgagatc aagcacgtg aaggacgat tacaacaaa 
      ctgtgcgaa gacgagtac gtgaaggcg tccaaacaa 
      acacaaatc ttcatcaga gattggttg gactcgcct 
      tggtcgggc ttcttcgag gggaaagac ccgctcaag 
      gtgaccccc accggggtg ccggaagcc accctcgcg 
      cacatcggg gccaaagtc tcctcgccg ccgccccca 
      aacctcgac ttcgtgctc cacaagggc ataactcgc 
      attctgaac caacgcgaa gcgatggtc aagaaccgg 
      caaatcgac tgggctttg ggggaggcg atggccatc 
      gggtccctc gtcaaggag ggcgtccac gtgcgcctc 
      tccggcgag gacgtcgag cgcgggacg ttctcgcac 
      cgccaccac gtctaccac caccagacc gtggacaaa 
      atgacctac cagcccctc ggtgacgtc ttcgacgcc 
      caagcgccc tacgtggtg tgcaacagc tcgctgtcc 
      gagtacggc gtcctgggc ttcgaggtg ggctatgcc 
      ctggccaac cccaactcc ctgatcctg tgggaagcc 
      cagttcgga gatttctac aacacggcg cagtgcata 
      atggaccag tttctgtgc tgtggccag gccaagtgg 
      atgcggcag atcggcctc gttgtgctc ctgccgcat 
      gggttcgac ggcatgggc ccggagcac tccagcggc 
      cggcctgag cgcttcctg cagaactgt gacgacgac 
      cccgacgtg gtcccgccc ccaggaccc gactttgct 
      attaaacaa ctccacgat tgcaactgg atcgtggcc 
      aattgttcc acacccgcc aacctcttc cacatctgg 
      cggcgccaa gtcgctcta ccattccgc aaacccttg 
      atcctgttc agtcccaaa aacctgcta aggcacccc 
      gagtgcaaa agctccttc gacgaaatg ctcgaaggg 
      acggaattc aaacgagtt atccccgac agtgggcca 
      gcgagtcag gacccgcaa aacgtcaag aaactcatc 
      ctttgcacc gggaaggtg tattacgat attatggac 
      gctttcaaa aagaaaaaa gtcgggaag gagatcgcc 
      gttacgaga gtcgagcaa ttgtcgccg tttccgtac 
      gatttgctt aaaaccgaa ttcgagaag tatccaaac 
      gcgaaaatt tgctgggct caagaagag cacaagaac 
      ggggggccg tatttgtac gttttggcg cggttgaac 
      acacttttg aataggtca cgcgaaatt cattatatt 
      gggagggcg gtgtccgca gccccggcc acgggaacc 
      aaagcagtg catctaaag gaagtcgaa atgttggcg 
      aacgaattt gtggcgctt tag
    mRNA sequence
    • >TC012622 RA
      cccttctaa atcatgtca agtttaaaa ctcctctcc 
      ttcttaaaa ttagcccca caatgcctc cgccactac 
      tcggaaacc tccttcctc actggaacc tcatctcaa 
      tacatcgaa gacatgtac aatgcttgg ctcaaagac 
      ccatccagc gtccacgtc tcctgggac tccttcttc 
      cgcaacacc agccaaggc ctcccccaa ccctaccac 
      gcccctccg aacctggcg ccctccaaa ggcgcccta 
      gtctcccaa atcgcggcc gtcgcaacc cccccacaa 
      accgccccc ctcgacgaa agaacaatc gaggaccat 
      ttagccgtc caggccgtc attcgcagc taccaagcc 
      cgcggccat ctagtcgcc aaactagac cctttggaa 
      atcatgttc aatgataaa accacgacc acggtgtcg 
      caaaccggg tcaccaccg gaggaaatc ctccgaaca 
      ttccgcctg gaaaacctg gacaaagtc tacaaactg 
      ccgtcgagc acgtacatc ggcgccgag ggcgaaaag 
      aaactccct ctccgtgag attttgcac cggctggag 
      ttggcttac tgccgccac atcggggtg gagtacatg 
      tacatcgat gatttggaa cagagggat tttatccgg 
      cgccggatg gaagcccca ggtgtgctt aaccagagc 
      acggtccag aaaaaactc accttgact aggcttatt 
      agaacggtc gagtttgag aatttcttg gccaagaaa 
      tgggcctcc gagaagcgg ttcgggatc gaaggctgc 
      gacatgttc attcctgga cttgaacaa atcatcgac 
      aagtcgacg gaacacggc gtcgagcat tttttcctc 
      gggttgagt cacaggggc cgtttgaac accctggct 
      aatattctt cggaagccg ctctaccag attttcaac 
      caatttgtg ccgctggag cccgcggat ttggggtcc 
      ggcgacgtg aagtaccac cacgggagt cacacccag 
      cgcaccaac cagctcacc cagaagcac tacaaagtc 
      acgctgctg gcgaaccca tcgcacttg gagagcgtc 
      aactcggtg gtgcttggg cgcacccgc gccgagcag 
      ttctacaaa ggcgacgat gagggcaag aagagtttg 
      gcgattttg atccacgga gacgccgct ttttctggc 
      caaggggtc aactacgag agctacggt ctgtcctac 
      ttgccccac tacaccaca cacggggct atctgtttc 
      attataaac aaccaggtg gggttcacc accgatcct 
      cggttttcc cggtcgtcg cgctactgc tccgacttg 
      ggcaaagtc gtgaacgcg cccattttc cacgtgaac 
      gccgacgac cccgagagc gttatccac gtgtgcaac 
      atcgcggcg gagtggagg gccaagttc cacaaagac 
      atcatcatc gacttggtc gggtaccgc cgccacggc 
      cacaacgaa gccgacgag cccatgttc acccagccc 
      ctgatgtac accaagatc aagagcatg gcctccatc 
      ggggacaag tactccagc gagttgctg aaggagaaa 
      gtggtgacg aaggacgag atcaagcac gtgaaggac 
      gattacaac aaactgtgc gaagacgag tacgtgaag 
      gcgtccaaa caaacacaa atcttcatc agagattgg 
      ttggactcg ccttggtcg ggcttcttc gaggggaaa 
      gacccgctc aaggtgacc cccaccggg gtgccggaa 
      gccaccctc gcgcacatc ggggccaaa gtctcctcg 
      ccgccgccc ccaaacctc gacttcgtg ctccacaag 
      ggcataact cgcattctg aaccaacgc gaagcgatg 
      gtcaagaac cggcaaatc gactgggct ttgggggag 
      gcgatggcc atcgggtcc ctcgtcaag gagggcgtc 
      cacgtgcgc ctctccggc gaggacgtc gagcgcggg 
      acgttctcg caccgccac cacgtctac caccaccag 
      accgtggac aaaatgacc taccagccc ctcggtgac 
      gtcttcgac gcccaagcg ccctacgtg gtgtgcaac 
      agctcgctg tccgagtac ggcgtcctg ggcttcgag 
      gtgggctat gccctggcc aaccccaac tccctgatc 
      ctgtgggaa gcccagttc ggagatttc tacaacacg 
      gcgcagtgc ataatggac cagtttctg tgctgtggc 
      caggccaag tggatgcgg cagatcggc ctcgttgtg 
      ctcctgccg catgggttc gacggcatg ggcccggag 
      cactccagc ggccggcct gagcgcttc ctgcagaac 
      tgtgacgac gaccccgac gtggtcccg cccccagga 
      cccgacttt gctattaaa caactccac gattgcaac 
      tggatcgtg gccaattgt tccacaccc gccaacctc 
      ttccacatc tggcggcgc caagtcgct ctaccattc 
      cgcaaaccc ttgatcctg ttcagtccc aaaaacctg 
      ctaaggcac cccgagtgc aaaagctcc ttcgacgaa 
      atgctcgaa gggacggaa ttcaaacga gttatcccc 
      gacagtggg ccagcgagt caggacccg caaaacgtc 
      aagaaactc atcctttgc accgggaag gtgtattac 
      gatattatg gacgctttc aaaaagaaa aaagtcggg 
      aaggagatc gccgttacg agagtcgag caattgtcg 
      ccgtttccg tacgatttg cttaaaacc gaattcgag 
      aagtatcca aacgcgaaa atttgctgg gctcaagaa 
      gagcacaag aacgggggg ccgtatttg tacgttttg 
      gcgcggttg aacacactt ttgaatagg tcacgcgaa 
      attcattat attgggagg gcggtgtcc gcagccccg 
      gccacggga accaaagca gtgcatcta aaggaagtc 
      gaaatgttg gcgaacgaa tttgtggcg ctttagtac 
      tgtatctcg taaaaaata aaagtttcg tcattgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY