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Gene information
General information
TC009747 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7215021..7219329
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC009747
      iBeetle screen iB_10667

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009747 RA
        MSDKKAIGG AVPPPSSAQ TFTPQPPPQ 
        ANQSVPTPQ PYGVVPGLP TASPYVIPG 
        QTQIYPQGP PPTYDQALT HPAAIVGQP 
        MYHPGTMYA AGYPTYLGY PTYAPMQYY 
        PSLGAYSYA MQPAQLRPT IMIPNGYDA 
        GARFDGIAQ PVLPPAPPG VPPSAAQLA 
        AMAGHQVAL GQKKNSFLA GGSDGGYTF 
        W
      • >TC009747 RB
        MTPPSAVLF SVVPGLPTA SPYVIPGQT 
        QIYPQGPPP TYDQALTHP AAIVGQPMY 
        HPGTMYAAG YPTYLGYPT YAPMQYYPS 
        LGAYSYAMQ PAQLRPTIM IPNGYDAGA 
        RFDGIAQPV LPPAPPGVP PSAAQLAAM 
        AGHQVALGQ KKNSFLAGG SDGGYTFW
      • >TC009747 RC
        MNNSKIRSE KFVQKYSYS VSRQSKIFL 
        HIFEQPIGG AVPPPSSAQ TFTPQPPPQ 
        ANQSVPTPQ PYGVVPGLP TASPYVIPG 
        QTQIYPQGP PPTYDQALT HPAAIVGQP 
        MYHPGTMYA AGYPTYLGY PTYAPMQYY 
        PSLGAYSYA MQPAQLRPT IMIPNGYDA 
        GARFDGIAQ PVLPPAPPG VPPSAAQLA 
        AMAGHQVAL GQKKNSFLA GGSDGGYTF 
        W
      CDS sequence
      • >TC009747 RA
        atgagcgat aaaaaagct atagggggc gccgtaccc 
        ccgccatca tctgcgcag acattcact cctcagcct 
        ccaccgcag gctaatcag tcggtacca acgccacaa 
        ccatatgga gtagttcct ggtttaccg accgcttcc 
        ccttacgtt atacctggc caaacccag atttaccca 
        caagggcct ccccccacc tacgatcag gctttgaca 
        cacccagct gcgatagtg gggcagccc atgtaccat 
        ccgggcacg atgtatgcc gccggatac cccacctac 
        ctcgggtac cccacttac gcccctatg cagtattat 
        ccctctctg ggcgcgtat tcgtacgcg atgcaacca 
        gcacagctg aggcccacg atcatgatt ccgaacggg 
        tacgacgcc ggggcgagg tttgatggg atagcccag 
        cccgtactt cctccggcc ccacccggg gttccgccc 
        tccgccgcg cagctagcg gcgatggcg ggccaccag 
        gtggctcta ggacagaag aaaaactct tttctggca 
        ggcggctcc gatggggga tacacattc tggtag
      • >TC009747 RB
        atgacgcca ccttccgct gttctattt tcagtagtt 
        cctggttta ccgaccgct tccccttac gttatacct 
        ggccaaacc cagatttac ccacaaggg cctcccccc 
        acctacgat caggctttg acacaccca gctgcgata 
        gtggggcag cccatgtac catccgggc acgatgtat 
        gccgccgga taccccacc tacctcggg taccccact 
        tacgcccct atgcagtat tatccctct ctgggcgcg 
        tattcgtac gcgatgcaa ccagcacag ctgaggccc 
        acgatcatg attccgaac gggtacgac gccggggcg 
        aggtttgat gggatagcc cagcccgta cttcctccg 
        gccccaccc ggggttccg ccctccgcc gcgcagcta 
        gcggcgatg gcgggccac caggtggct ctaggacag 
        aagaaaaac tcttttctg gcaggcggc tccgatggg 
        ggatacaca ttctggtag
      • >TC009747 RC
        atgaataac tcgaaaata cgatctgaa aaatttgtg 
        caaaaatat tcctattct gtgagtcga caatcgaaa 
        atatttttg catatcttc gagcaacct atagggggc 
        gccgtaccc ccgccatca tctgcgcag acattcact 
        cctcagcct ccaccgcag gctaatcag tcggtacca 
        acgccacaa ccatatgga gtagttcct ggtttaccg 
        accgcttcc ccttacgtt atacctggc caaacccag 
        atttaccca caagggcct ccccccacc tacgatcag 
        gctttgaca cacccagct gcgatagtg gggcagccc 
        atgtaccat ccgggcacg atgtatgcc gccggatac 
        cccacctac ctcgggtac cccacttac gcccctatg 
        cagtattat ccctctctg ggcgcgtat tcgtacgcg 
        atgcaacca gcacagctg aggcccacg atcatgatt 
        ccgaacggg tacgacgcc ggggcgagg tttgatggg 
        atagcccag cccgtactt cctccggcc ccacccggg 
        gttccgccc tccgccgcg cagctagcg gcgatggcg 
        ggccaccag gtggctcta ggacagaag aaaaactct 
        tttctggca ggcggctcc gatggggga tacacattc 
        tggtag
      mRNA sequence
      • >TC009747 RA
        tgaaagaag tgaagtttc acaaaacaa gtgtaataa 
        ttgtaatta attttgtac gataattgt gattattag 
        agtcgccaa cgaaaatga gcgataaaa aagctatag 
        ggggcgccg tacccccgc catcatctg cgcagacat 
        tcactcctc agcctccac cgcaggcta atcagtcgg 
        taccaacgc cacaaccat atggagtag ttcctggtt 
        taccgaccg cttcccctt acgttatac ctggccaaa 
        cccagattt acccacaag ggcctcccc ccacctacg 
        atcaggctt tgacacacc cagctgcga tagtggggc 
        agcccatgt accatccgg gcacgatgt atgccgccg 
        gatacccca cctacctcg ggtacccca cttacgccc 
        ctatgcagt attatccct ctctgggcg cgtattcgt 
        acgcgatgc aaccagcac agctgaggc ccacgatca 
        tgattccga acgggtacg acgccgggg cgaggtttg 
        atgggatag cccagcccg tacttcctc cggccccac 
        ccggggttc cgccctccg ccgcgcagc tagcggcga 
        tggcgggcc accaggtgg ctctaggac agaagaaaa 
        actcttttc tggcaggcg gctccgatg ggggataca 
        cattctggt aggaaacgg ccctaggtt taagcaatt 
        caaattagg ttaacgaaa ttctttatt ttatcttaa 
        cgactagca aggtctgtc tagtctata tcatttcaa 
        gtactaaat gtcggaagt gagccgtct aagtcactt 
        ttgtacatg aattgctga caacttaaa aaaacatat 
        taaaatgag ttattttca aaacgaaaa aaatattag 
        agtacaagt tagagtgat ctgaaactg tccaatgac 
        ggttctagg taatctgtt attgtttga tgatattaa 
        gacgtttcc tgtttgcat aacgtggcg tttctagga 
        ttacgtgac aaattatct tcgttcttt ctttttttg 
        ttgaatatc tattaatgt gcaataaac gttgttgac 
        attgtaatt tattgtttt gtaaaattt taggttttg 
        ttgcgaagt ttttaggta cgtaaaatt ttataggca 
        ataagttga ttggtgatg cgactggtt cccaactaa 
        aaatgtgcg attatattt tttatttgg gaatgagtc 
        ttcttttta gagatattt cttttattt tagttttgg 
        aaagcaagt ttgaagtca gttctgccg attgtacgg 
        caacacttt catttccta aaaaaataa aataaaaga 
        actatcaag acgatgcag cttaatgaa tatttacta 
        gtcaataac aagtaaaac aatcgatag aatattaat 
        tgaaaaaaa tataaaaca ccaacaaaa acttcttgt 
        tacttgcca taattttgt tatactaat gttctgttg 
        ttacttgaa acttttaca atgaaattg aactattgt 
        gagactgtg aagatctat tgtgaaaaa tgtaatgct 
        gtaatattg aatgagtgt ttcttttac ccattccca 
        ggattccca ataaaaata ataaaaata c
      • >TC009747 RB
        tgatttttt cgtattctc attgagctg tgattattt 
        ttaagcgtt tgttagttt tgttaggtt ggtggtggt 
        gcacatgcg cgtcttttg acgttttat tcgtgtggt 
        ggtgaaaga agtgaagtt tcacaaaac aagtgtaat 
        aattgtaat taattttgt acgataatt gtgattatt 
        agagtcgcc aacgaaaat gagcgataa aaaaggtaa 
        gttgtggtt ttggtgccg ttaggaata taaatcgcc 
        tcggtgttc acccccttg gagggctac acgggcccc 
        atccgcgat ccgccgtat ttcggcaaa aaagacgat 
        ttcgaaatc tcgggggca gattttggc aaaaatttt 
        gcatagttc cgtgcagga tgttccaat caatactaa 
        cagttaggt tatctttgt ctgaatcag gtaacttcc 
        atgttcttg ctcattctg ttcccatgc ctttttaat 
        agccttgtc ctacattgc aaaatactg cagtaggta 
        atgttaggg tttttaata aactgaatg tgatttagg 
        gcgatttat atgatgacg atgtgttat tccagcgcc 
        tggttttaa atcgactgg ggaggagcc gcggaccgc 
        aaaggatct cggactgca aacgataca gtacatatt 
        tattttcat gtgttttaa acagtggct gatgcttgt 
        tggttatac ccgctcaac gattttacc ttcactaga 
        tttttggtt gggattgca tacaacact gcatttttt 
        caaccagaa tgtttgctt cttcccaca gtttttttt 
        agttcagta cgggtcgac aatgcagtg ttgtaacaa 
        ttactgtag ctgaagatg taaaaattc tcagtggtc 
        caacattag acatttgtg gtctatcac tgcatgatg 
        aagtccaaa aaatacgca actcccact cgggcccgc 
        ttttattta agcagcgtt ttaaccaac agatggtta 
        attttttct tttgcgttg gcgtccata aggtcataa 
        ctcgtgaaa aattcatca aacgagtcg caagagcgg 
        aaagaatgc cattcgaga catgcaatc ttaaactag 
        ataagaaaa tcgattgct ggaatacca attcgtgac 
        aaaagtttc cgcgtttag aaagtttcg gctgcttgc 
        gacaagttt taaatcgaa agcatttcc acttgcagc 
        ataattaaa tatttatat tcgaggaga ttgtctttg 
        ttttgttat tccgtttca gtttttgca agccatttt 
        aattaaagg aagttgaga gaaccagta gttctcaaa 
        accagtaaa tttcgtaca ttgtaagat tttgtgcgt 
        ctcccgttt gtctaataa ataaagtct ggggtccga 
        gtgcgtcgt tgcatattt tttgttgtg tgtgggtgt 
        agggtggta cttactgga ttcacccgg tgtttgcta 
        caatgttgg ctaaaaccg ctgcagcac ctctacgtc 
        gctaatata tcgtaaaat cagttccac ttatcggat 
        gaaaatttt aaattttac aaactctga taagtgggg 
        cgttctctg ccacaggtc tacgggatt ttgtattta 
        aaatgtttg aattacctt attaatatt ataacttag 
        tatttactt gtgcatggt gctagggta aattgcttt 
        cgaacttcg agtcaatag gtgtaacta aaaatccga 
        cgaagcaat gtaccaaaa cactcgatc taatgattt 
        ttatttttg catgcctta tggtgtaca tattagggc 
        atgaacata tttccaaat tggtaaaaa cgtcttcag 
        aaaaacaga ttctttctc taaaggaaa aaatctgtt 
        ggatgatta cacttgatt tcttaatca agtcccgct 
        ttcatatgt ccaaaataa caacattaa gcttgtttt 
        tagatattt cgcatgcta ttattcaac ggttttagc 
        agacggcat cggagtgta aaatttctg ctgaaactg 
        tctttttat ttattttct tgcatgatt ttattttat 
        agttatctc aacaaatac acttctggc attccttgt 
        ttgtaaccc gagaagtgt atttttcac ttttttctt 
        tcttatttc ttttgtttg catgtttta tgcacataa 
        agatcattc tgcattagt cagttcctt ccaaagttc 
        cctcgggtt gaaatgatg tgatgttat cttttgcat 
        atgaggaat aagtgctgt cctaaatcc agataaatt 
        tttcgtgag atgcagaat gaatccaat gcatgcttt 
        cagatataa tatcttttc ctatgaata acgtacgga 
        aataatcta aatgtaaat aatagttat aggagtatt 
        ttgtaatct agtcgaaaa tacgatctg aaaaatttg 
        tgcaaaaat attcctatt ctgtgagtc gacaatcga 
        aaatatttt tgcatatct tcgagcaac gtcacaaat 
        tttcagtgt tttccacta aaaaagcat taatttctt 
        ttctttgct tcattttag atttctttg atcaattaa 
        taatcattt gcaaattaa aaaccaaaa aaataacca 
        aaatcacaa aacagttgg tttggtttt tatttttca 
        ttacggcgt tttacgatc gaatgttta aaacggtat 
        ttaattcgg tttgcagct atagggggc gccgtaccc 
        ccgccatca tctgcgcag acattcact cctcagcct 
        ccaccgcag gctaatcag tcggtacca acgccacaa 
        ccatatgga ggtataatc cttccaaaa tacaaccat 
        gacgccacc ttccgctgt tctattttc agtagttcc 
        tggtttacc gaccgcttc cccttacgt tatacctgg 
        ccaaaccca gatttaccc acaagggcc tccccccac 
        ctacgatca ggctttgac acacccagc tgcgatagt 
        ggggcagcc catgtacca tccgggcac gatgtatgc 
        cgccggata ccccaccta cctcgggta ccccactta 
        cgcccctat gcagtatta tccctctct gggcgcgta 
        ttcgtacgc gatgcaacc agcacagct gaggcccac 
        gatcatgat tccgaacgg gtacgacgc cggggcgag 
        gtttgatgg gatagccca gcccgtact tcctccggc 
        cccacccgg ggttccgcc ctccgccgc gcagctagc 
        ggcgatggc gggccacca ggtggctct aggacagaa 
        gaaaaactc ttttctggc aggcggctc cgatggggg 
        atacacatt ctggtagga aacggccct aggtttaag 
        caattcaaa ttaggttaa cgaaattct ttattttat 
        cttaacgac tagcaaggt ctgtctagt ctatatcat 
        ttcaagtac taaatgtcg gaagtgagc cgtctaagt 
        cacttttgt acatgaatt gctgacaac ttaaaaaaa 
        catattaaa atgagttat tttcaaaac gaaaaaaat 
        attagagta caagttaga gtgatctga aactgtcca 
        atgacggtt ctaggtaat ctgttattg tttgatgat 
        attaagacg tttcctgtt tgcataacg tggcgtttc 
        taggattac gtgacaaat tatcttcgt tctttcttt 
        ttttgttga atatctatt aatgtgcaa taaacgttg 
        ttgacattg taatttatt gttttgtaa aattttagg 
        ttttgttgc gaagttttt aggtacgta aaattttat 
        aggcaataa gttgattgg tgatgcgac tggttccca 
        actaaaaat gtgcgatta tatttttta tttgggaat 
        gagtcttct ttttagaga tatttcttt tattttagt 
        tttggaaag caagtttga agtcagttc tgccgattg 
        tacggcaac actttcatt tcctaaaaa aataaaata 
        aaagaacta tcaagacga tgcagctta atgaatatt 
        tactagtca ataacaagt aaaacaatc gatagaata 
        ttaattgaa aaaaatata aaacaccaa caaaaactt 
        cttgttact tgccataat tttgttata ctaatgttc 
        tgttgttac ttgaaactt ttacaatga aattgaact 
        attgtgaga ctgtgaaga tctattgtg aaaaatgta 
        atgctgtaa tattgaatg agtgtttct tttacccat 
        tcccaggat tcccaataa aaataataa aaatac
      • >TC009747 RC
        tgatttttt cgtattctc attgagctg tgattattt 
        ttaagcgtt tgttagttt tgttaggtt ggtggtggt 
        gcacatgcg cgtcttttg acgttttat tcgtgtggt 
        ggtgaaaga agtgaagtt tcacaaaac aagtgtaat 
        aattgtaat taattttgt acgataatt gtgattatt 
        agagtcgcc aacgaaaat gagcgataa aaaaggtaa 
        gttgtggtt ttggtgccg ttaggaata taaatcgcc 
        tcggtgttc acccccttg gagggctac acgggcccc 
        atccgcgat ccgccgtat ttcggcaaa aaagacgat 
        ttcgaaatc tcgggggca gattttggc aaaaatttt 
        gcatagttc cgtgcagga tgttccaat caatactaa 
        cagttaggt tatctttgt ctgaatcag gtaacttcc 
        atgttcttg ctcattctg ttcccatgc ctttttaat 
        agccttgtc ctacattgc aaaatactg cagtagggc 
        gatttatat gatgacgat gtgttattc cagcgcctg 
        gttttaaat cgactgggg aggagccgc ggaccgcaa 
        aggatctcg gactgcaaa cgatacagt acatattta 
        ttttcatgt gttttaaac agtggctga tgcttgttg 
        gttataccc gctcaacga ttttacctt cactagatt 
        tttggttgg gattgcata caacactgc attttttca 
        accagaatg tttgcttct tcccacagt tttttttag 
        ttcagtacg ggtcgacaa tgcagtgtt gtaacaatt 
        actgtagct gaagatgta aaaattctc agtggtcca 
        acattagac atttgtggt ctatcactg catgatgaa 
        gtccaaaaa atacgcaac tcccactcg ggcccgctt 
        ttatttaag cagcgtttt aaccaacag atggttaat 
        tttttcttt tgcgttggc gtccataag gtcataact 
        cgtgaaaaa ttcatcaaa cgagtcgca agagcggaa 
        agaatgcca ttcgagaca tgcaatctt aaactagat 
        aagaaaatc gattgctgg aataccaat tcgtgacaa 
        aagtttccg cgtttagaa agtttcggc tgcttgcga 
        caagtttta aatcgaaag catttccac ttgcagcat 
        aattaaata tttatattc gaggagatt gtctttgtt 
        ttgttattc cgtttcagt ttttgcaag ccattttaa 
        ttaaaggaa gttgagaga accagtagt tctcaaaac 
        cagtaaatt tcgtacatt gtaagattt tgtgcgtct 
        cccgtttgt ctaataaat aaagtctgg ggtccgagt 
        gcgtcgttg catattttt tgttgtgtg tgggtgtag 
        ggtggtact tactggatt cacccggtg tttgctaca 
        atgttggct aaaaccgct gcagcacct ctacgtcgc 
        taatatatc gtaaaatca gttccactt atcggatga 
        aaattttaa attttacaa actctgata agtggggcg 
        ttctctgcc acaggtcta cgggatttt gtatttaaa 
        atgtttgaa ttaccttat taatattat gtgactaat 
        ctagattaa gtaattatt ttttttgat agaacttag 
        tatttactt gtgcatggt gctagggta aattgcttt 
        cgaacttcg agtcaatag gtgtaacta aaaatccga 
        cgaagcaat gtaccaaaa cactcgatc taatgattt 
        ttatttttg catgcctta tggtgtaca tattagggc 
        atgaacata tttccaaat tggtaaaaa cgtcttcag 
        aaaaacaga ttctttctc taaaggaaa aaatctgtt 
        ggatgatta cacttgatt tcttaatca agtcccgct 
        ttcatatgt ccaaaataa caacattaa gcttgtttt 
        tagatattt cgcatgcta ttattcaac ggttttagc 
        agacggcat cggagtgta aaatttctg ctgaaactg 
        tctttttat ttattttct tgcatgatt ttattttat 
        agttatctc aacaaatac acttctggc attccttgt 
        ttgtaaccc gagaagtgt atttttcac ttttttctt 
        tcttatttc ttttgtttg catgtttta tgcacataa 
        agatcattc tgcattagt cagttcctt ccaaagttc 
        cctcgggtt gaaatgatg tgatgttat cttttgcat 
        atgaggaat aagtgctgt cctaaatcc agataaatt 
        tttcgtgag atgcagaat gaatccaat gcatgcttt 
        cagatataa tatcttttc ctatgaata actcgaaaa 
        tacgatctg aaaaatttg tgcaaaaat attcctatt 
        ctgtgagtc gacaatcga aaatatttt tgcatatct 
        tcgagcaac ctatagggg gcgccgtac ccccgccat 
        catctgcgc agacattca ctcctcagc ctccaccgc 
        aggctaatc agtcggtac caacgccac aaccatatg 
        gagtagttc ctggtttac cgaccgctt ccccttacg 
        ttatacctg gccaaaccc agatttacc cacaagggc 
        ctcccccca cctacgatc aggctttga cacacccag 
        ctgcgatag tggggcagc ccatgtacc atccgggca 
        cgatgtatg ccgccggat accccacct acctcgggt 
        accccactt acgccccta tgcagtatt atccctctc 
        tgggcgcgt attcgtacg cgatgcaac cagcacagc 
        tgaggccca cgatcatga ttccgaacg ggtacgacg 
        ccggggcga ggtttgatg ggatagccc agcccgtac 
        ttcctccgg ccccacccg gggttccgc cctccgccg 
        cgcagctag cggcgatgg cgggccacc aggtggctc 
        taggacaga agaaaaact cttttctgg caggcggct 
        ccgatgggg gatacacat tctggtagg aaacggccc 
        taggtttaa gcaattcaa attaggtta acgaaattc 
        tttatttta tcttaacga ctagcaagg tctgtctag 
        tctatatca tttcaagta ctaaatgtc ggaagtgag 
        ccgtctaag tcacttttg tacatgaat tgctgacaa 
        cttaaaaaa acatattaa aatgagtta ttttcaaaa 
        cgaaaaaaa tattagagt acaagttag agtgatctg 
        aaactgtcc aatgacggt tctaggtaa tctgttatt 
        gtttgatga tattaagac gtttcctgt ttgcataac 
        gtggcgttt ctaggatta cgtgacaaa ttatcttcg 
        ttctttctt tttttgttg aatatctat taatgtgca 
        ataaacgtt gttgacatt gtaatttat tgttttgta 
        aaattttag gttttgttg cgaagtttt taggtacgt 
        aaaatttta taggcaata agttgattg gtgatgcga 
        ctggttccc aactaaaaa tgtgcgatt atatttttt 
        atttgggaa tgagtcttc tttttagag atatttctt 
        ttattttag ttttggaaa gcaagtttg aagtcagtt 
        ctgccgatt gtacggcaa cactttcat ttcctaaaa 
        aaataaaat aaaagaact atcaagacg atgcagctt 
        aatgaatat ttactagtc aataacaag taaaacaat 
        cgatagaat attaattga aaaaaatat aaaacacca 
        acaaaaact tcttgttac ttgccataa ttttgttat 
        actaatgtt ctgttgtta cttgaaact tttacaatg 
        aaattgaac tattgtgag actgtgaag atctattgt 
        gaaaaatgt aatgctgta atattgaat gagtgtttc 
        ttttaccca ttcccagga ttcccaata aaaataata 
        aaaatac
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