Status
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Gene information
General information
TC011534 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3646813..3651302
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011534
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10664

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011534 RA
      MSHLMRILR ADDQVRESQ QSRATSTPR DNNSPNFSS 
      PSVSTIPRD EEPMEDGDS DENWTKGTS DDADSSLCE 
      SLAEVSISS RQTAKRLKW ENFWRSLKP KNTQSLYYK 
      IMTQPEKLD SITVELIDT YKTNKKFAI FCILQLFVD 
      LSGYKKLSI GEVYDFERG NSYNVVQRM ESDLLDEEL 
      KKTGKFLFM KPTSFVKDA EHIFAQFLD HLLIQAFKS 
      QVLFDEVFA AHVFEFVRC MCLSPVSGP CVTGLITVI 
      NLITSFLKL HQNLIALSD EIDRNSSSE TISVIQRKI 
      EDHIDFLYF VFDKHCLLS DKRGALVKI RSAQETFQW 
      IRLYPETFI LKRRCLGPL LKMTLDQHE FVRLAALKT 
      IEKLIHCQE ISDVLKQRI EMCLKFASG RIVDVSPQV 
      AVMAIHVFT VATESYFDK ITTEHKNKI IQEIYFKDV 
      ILGKAAGEF LVAYLHRPN TTGEHFLFS LVLAAFSKP 
      EFVEQLPIF LESVFEFAD ELKDWPLFV QVFLNDDIR 
      LGAKTALLK ILNECVRFV LTGKFSYSR QVSQVLGRC 
      DDEEHIQIA NAIIPNFTQ LLILFQAEK VSLQKLIEL 
      LSFIDYSVC RVNGLNDDF SKIFSILKE MFKATADRT 
      LLQNITNVF AVFCEQRHY YKATLVVES LKTWIESLP 
      EDLNQMTPD CDKTLLIQI RNKSLKAST LFSRFDLTK 
      VLQWQDIFP VLETNFFKV LKYLVVCCK WHLIWRLRQ 
      FMRLPKDDN GSEPISPLL YNDCKEFVY ECLKMFTQE 
      KSNSDLFLV LETLCDLYT DFENELKRK REYSHFVIK 
      ITEPRSLEI LFNFVVQHI INNKEMPLK ERQKLLRKV 
      INLLYLDVI PCEYFSKIL RFYHTHNNE FGYLMDHIF 
      GQLKITPPA LPLIVMHTL AEIYQEILE KRQIVDLRT 
      EETKFLKKL AKKFASVKE MRSNQDILK FIIMALNFT 
      CRTEQHYPF LSFAKYFAK HLNEDGKSD AYEIFRKSV 
      PKEAEKNEI FLLFAKTLK
    CDS sequence
    • >TC011534 RA
      atgtctcat ttaatgaga attttgcgt gctgatgac 
      caagttcgc gaatctcaa cagtcccgg gccaccagc 
      actcctcga gacaataat tctcccaac ttctcttcc 
      ccaagcgtt tccaccatt ccaagggac gaagaaccg 
      atggaagat ggagacagt gacgaaaat tggaccaaa 
      ggaacaagc gatgacgcg gattcatct ctttgcgaa 
      tctcttgcc gaagtttca atcagcagt cgtcaaact 
      gcgaaacga ctcaaatgg gagaacttt tggagatcg 
      ctgaaaccg aaaaacacg cagagtctg tattacaaa 
      ataatgacc caacctgag aaactagac agcataaca 
      gttgagctg atagacacc tacaagact aataaaaaa 
      tttgccatt ttttgcata ctccagctt tttgttgac 
      ctgagtggt tataaaaaa ttatcaatc ggcgaagtt 
      tacgatttt gagcgcgga aattcgtat aatgtcgtc 
      caacgaatg gaatctgac ttactggac gaagaacta 
      aaaaaaacg ggaaaattc ttgtttatg aaaccaaca 
      agttttgta aaagacgct gaacatatt tttgcccag 
      tttttagac catttatta atccaggca ttcaagtct 
      caagtgtta tttgatgaa gtttttgca gcacacgtt 
      ttcgaattc gttaggtgt atgtgttta agtccagta 
      agtggtccc tgtgttact ggtcttata acagtaata 
      aatttgata acgtcgttt ctgaaactg caccaaaat 
      ttaattgct ttaagtgac gaaattgat aggaatagt 
      tccagtgaa acgatatca gtaatacaa agaaaaatt 
      gaagaccat attgatttt ctttacttc gtttttgat 
      aaacattgt ttgctttct gataaaaga ggggctttg 
      gtaaaaata cgatcagct caagaaacg ttccagtgg 
      atccgtttg taccctgaa actttcata ctgaaacgt 
      cgttgtttg gggccttta ttgaaaatg actcttgac 
      cagcacgaa ttcgtccgt ttggcggct ctcaaaact 
      attgaaaaa ttgatccat tgtcaggaa atttcagac 
      gtcctgaag caacgaatt gagatgtgc ttgaaattt 
      gcttccggt cgcattgtc gacgttagt cctcaagtg 
      gctgttatg gcaatacat gtcttcacc gtagccaca 
      gagagctac tttgacaaa attaccacg gaacataag 
      aacaaaatc attcaggag atctatttc aaagatgta 
      attttgggc aaagcggct ggggaattc ctagtggct 
      tatcttcat cgtccaaac accactggg gaacacttt 
      ttgttctca cttgttcta gcagcattc tcaaaaccg 
      gagtttgta gaacaactc ccaattttt ctcgaatca 
      gtttttgaa tttgccgat gaattgaaa gactggccg 
      ttgtttgtg caagtgttt ctaaacgat gatattcgt 
      ttaggcgcc aaaacagcc cttcttaaa attttgaat 
      gagtgtgtg aggtttgtc ctcactgga aaattctct 
      tactccaga caggtctca caagtttta ggacggtgt 
      gacgatgaa gagcatatt caaatagca aatgctata 
      attcctaat ttcacgcag ttgctaata ctgtttcaa 
      gccgagaaa gtgagcttg caaaaatta attgaactc 
      ttgtctttt attgattat agtgtttgt cgtgttaat 
      ggcctgaac gatgatttc agtaaaatt ttttccata 
      ttaaaagaa atgtttaaa gcaactgct gatcgaacc 
      ttgctccaa aatataaca aacgtgttt gctgttttc 
      tgtgaacag cgccactac tacaaggct actcttgtg 
      gttgaaagt ttaaaaaca tggatcgag tcattacct 
      gaagatttg aaccaaatg actcccgat tgtgacaaa 
      acgttactg atacaaata agaaacaaa tcgttgaaa 
      gcttccact ttattttca cgttttgat ttgacaaaa 
      gttctccaa tggcaagac attttccca gtcttggaa 
      actaacttc ttcaaagtg ctcaaatat ttggtggtt 
      tgttgcaag tggcacttg atttggcgt ttgcgccaa 
      tttatgcga ctgcctaaa gacgataat ggaagtgaa 
      cccatcagt cctctcctc tacaacgac tgcaaagag 
      tttgtttat gaatgtttg aaaatgttc acacaagag 
      aagagcaat tccgactta ttcttggtt ttggaaact 
      ttgtgcgat ctttacacc gactttgaa aacgaactc 
      aaacgaaaa cgtgaatac tctcatttt gtaattaaa 
      attactgag cccaggagt ttggaaatt ttatttaat 
      ttcgtagtg cagcacata attaacaat aaagagatg 
      cctttgaaa gaacgccaa aaactgcta aggaaagtt 
      atcaatctt ttgtatctg gatgtaatt ccttgtgaa 
      tatttttcg aagatatta cgtttctat cacacgcac 
      aacaacgag tttggttat ttaatggac cacatattt 
      ggtcaatta aaaataacc cctccagcg ttgcctcta 
      atagtaatg catacattg gcagaaatt tatcaggaa 
      attttggag aaacgccaa attgttgat cttcggact 
      gaagaaaca aaattttta aagaaattg gctaaaaag 
      ttcgcttct gttaaagaa atgagatcg aatcaggat 
      attctcaaa tttattatc atggcccta aatttcacc 
      tgcagaact gaacaacat tatcctttt ctgagtttc 
      gcaaaatat tttgcaaaa catttaaat gaagatggg 
      aaaagcgat gcgtacgaa atattcagg aaatcggtt 
      cccaaagaa gcagaaaag aatgagata tttttgctt 
      ttcgcaaaa actctcaag tag
    mRNA sequence
    • >TC011534 RA
      tcattggcc aattaattt cgtccccat ttgtgcgca 
      tctaacatt ctttcgtgg cggtaaact ctattcaag 
      tgaatattt tcgtgcaaa aatgtctca tttaatgag 
      aattttgcg tgctgatga ccaagttcg cgaatctca 
      acagtcccg ggccaccag cactcctcg agacaataa 
      ttctcccaa cttctcttc cccaagcgt ttccaccat 
      tccaaggga cgaagaacc gatggaaga tggagacag 
      tgacgaaaa ttggaccaa aggaacaag cgatgacgc 
      ggattcatc tctttgcga atctcttgc cgaagtttc 
      aatcagcag tcgtcaaac tgcgaaacg actcaaatg 
      ggagaactt ttggagatc gctgaaacc gaaaaacac 
      gcagagtct gtattacaa aataatgac ccaacctga 
      gaaactaga cagcataac agttgagct gatagacac 
      ctacaagac taataaaaa atttgccat tttttgcat 
      actccagct ttttgttga cctgagtgg ttataaaaa 
      attatcaat cggcgaagt ttacgattt tgagcgcgg 
      aaattcgta taatgtcgt ccaacgaat ggaatctga 
      cttactgga cgaagaact aaaaaaaac gggaaaatt 
      cttgtttat gaaaccaac aagttttgt aaaagacgc 
      tgaacatat ttttgccca gtttttaga ccatttatt 
      aatccaggc attcaagtc tcaagtgtt atttgatga 
      agtttttgc agcacacgt tttcgaatt cgttaggtg 
      tatgtgttt aagtccagt aagtggtcc ctgtgttac 
      tggtcttat aacagtaat aaatttgat aacgtcgtt 
      tctgaaact gcaccaaaa tttaattgc tttaagtga 
      cgaaattga taggaatag ttccagtga aacgatatc 
      agtaataca aagaaaaat tgaagacca tattgattt 
      tctttactt cgtttttga taaacattg tttgctttc 
      tgataaaag aggggcttt ggtaaaaat acgatcagc 
      tcaagaaac gttccagtg gatccgttt gtaccctga 
      aactttcat actgaaacg tcgttgttt ggggccttt 
      attgaaaat gactcttga ccagcacga attcgtccg 
      tttggcggc tctcaaaac tattgaaaa attgatcca 
      ttgtcagga aatttcaga cgtcctgaa gcaacgaat 
      tgagatgtg cttgaaatt tgcttccgg tcgcattgt 
      cgacgttag tcctcaagt ggctgttat ggcaataca 
      tgtcttcac cgtagccac agagagcta ctttgacaa 
      aattaccac ggaacataa gaacaaaat cattcagga 
      gatctattt caaagatgt aattttggg caaagcggc 
      tggggaatt cctagtggc ttatcttca tcgtccaaa 
      caccactgg ggaacactt tttgttctc acttgttct 
      agcagcatt ctcaaaacc ggagtttgt agaacaact 
      cccaatttt tctcgaatc agtttttga atttgccga 
      tgaattgaa agactggcc gttgtttgt gcaagtgtt 
      tctaaacga tgatattcg tttaggcgc caaaacagc 
      ccttcttaa aattttgaa tgagtgtgt gaggtttgt 
      cctcactgg aaaattctc ttactccag acaggtctc 
      acaagtttt aggacggtg tgacgatga agagcatat 
      tcaaatagc aaatgctat aattcctaa tttcacgca 
      gttgctaat actgtttca agccgagaa agtgagctt 
      gcaaaaatt aattgaact cttgtcttt tattgatta 
      tagtgtttg tcgtgttaa tggcctgaa cgatgattt 
      cagtaaaat tttttccat attaaaaga aatgtttaa 
      agcaactgc tgatcgaac cttgctcca aaatataac 
      aaacgtgtt tgctgtttt ctgtgaaca gcgccacta 
      ctacaaggc tactcttgt ggttgaaag tttaaaaac 
      atggatcga gtcattacc tgaagattt gaaccaaat 
      gactcccga ttgtgacaa aacgttact gatacaaat 
      aagaaacaa atcgttgaa agcttccac tttattttc 
      acgttttga tttgacaaa agttctcca atggcaaga 
      cattttccc agtcttgga aactaactt cttcaaagt 
      gctcaaata tttggtggt ttgttgcaa gtggcactt 
      gatttggcg tttgcgcca atttatgcg actgcctaa 
      agacgataa tggaagtga acccatcag tcctctcct 
      ctacaacga ctgcaaaga gtttgttta tgaatgttt 
      gaaaatgtt cacacaaga gaagagcaa ttccgactt 
      attcttggt tttggaaac tttgtgcga tctttacac 
      cgactttga aaacgaact caaacgaaa acgtgaata 
      ctctcattt tgtaattaa aattactga gcccaggag 
      tttggaaat tttatttaa tttcgtagt gcagcacat 
      aattaacaa taaagagat gcctttgaa agaacgcca 
      aaaactgct aaggaaagt tatcaatct tttgtatct 
      ggatgtaat tccttgtga atatttttc gaagatatt 
      acgtttcta tcacacgca caacaacga gtttggtta 
      tttaatgga ccacatatt tggtcaatt aaaaataac 
      ccctccagc gttgcctct aatagtaat gcatacatt 
      ggcagaaat ttatcagga aattttgga gaaacgcca 
      aattgttga tcttcggac tgaagaaac aaaattttt 
      aaagaaatt ggctaaaaa gttcgcttc tgttaaaga 
      aatgagatc gaatcagga tattctcaa atttattat 
      catggccct aaatttcac ctgcagaac tgaacaaca 
      ttatccttt tctgagttt cgcaaaata ttttgcaaa 
      acatttaaa tgaagatgg gaaaagcga tgcgtacga 
      aatattcag gaaatcggt tcccaaaga agcagaaaa 
      gaatgagat atttttgct tttcgcaaa aactctcaa 
      gtagataag ttttttaac aaaataaat attcgtctt 
      gtttgaaat attaaatgt aattgttag cgttaaaag 
      agtttaaaa atttgttac gacttcgtc acttaactg 
      ttttttttt tgttttgaa gaacctatt atgattttt 
      aagatgcca tccctgaaa cattatctt tcttttaat 
      tttaatatt tttttatgt tactgtagc agagttttt 
      taccactga tttcggtcc tgtataact agagaaaag 
      gccaaaaaa gaatgaaac aattaaaac aaaaagtta 
      ttcaataat atcgttatc taataaacc ctttaacat 
      tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY