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Gene information
General information
TC013881 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 2131880..2133307
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013881
    iBeetle screen iB_10662

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013881 RA
      MARNLCQFL KIDVRLLLP SLHLNARLY SASPVKKSE 
      NVAPVYTKR DDEYKMTKK GAVSTLQKL LRLKTFDAF 
      NIVLNNDFF NNVSKATLK GNYALLVQK GITPETILK 
      YPELLTVSG TEQKLQIIE ALKYDINEV AILLLLNIP 
      SLKKLEENE VDPEGSTIT SLSSLLNID HQKACEYLA 
      RKPFLCFLS NDKITRKIE ILKSYGIPL EEICNDLWV 
      LRYREKLIE ERLSRAKKY NIGAAKTWM VRAKQEVFQ 
      VYIQRRSDN KSILGNSSL AEYLSERLK CSVEVANYM 
      IIKQPALQN KSLKKLKEL IDLLYAEVW KRRRKGCKN 
      SLKRGLKSI VYPC
    CDS sequence
    • >TC013881 RA
      atggctaga aatttgtgt cagtttttg aaaatcgat 
      gttcgacta ttgcttcct tcactgcat ttaaatgcg 
      aggttatat tctgcaagt cctgttaaa aaatcggaa 
      aatgtggct ccggtttac accaagaga gacgatgag 
      tataaaatg accaagaaa ggggctgtt agtactttg 
      cagaagctg ttgcgactt aaaactttt gacgcgttt 
      aacattgtg ttgaataat gattttttc aacaatgtg 
      tctaaagca actttaaaa ggcaattat gcacttctt 
      gtgcagaaa ggaataaca ccagaaact attttgaag 
      tatccagaa ttgttaaca gtgtcggga accgagcag 
      aaactacag attatagag gcgctaaaa tatgatata 
      aatgaagtt gcaattctt ttattacta aacatacct 
      agtcttaaa aaattagaa gagaatgag gtggatcca 
      gaagggagc actataacg agtctttct tcactttta 
      aatatagac catcagaaa gcgtgtgaa tatttggca 
      cgaaaacct tttctgtgt tttctttcg aacgataaa 
      atcacgaga aaaatagaa atactaaaa agttatggc 
      atcccatta gaagaaatc tgcaacgac ttgtgggtt 
      ttaagatac agagaaaaa ttaatagag gaacgttta 
      agtcgagca aagaaatac aacatcgga gctgcaaaa 
      acttggatg gtgcgagcc aaacaagaa gtgtttcaa 
      gtctatatc caaagacgt tctgacaat aagtcgatt 
      ctgggcaac agttccctc gccgagtac ttatcagaa 
      cgcctcaaa tgcagcgta gaagtggca aattacatg 
      ataattaaa cagccagcc ctccaaaac aaaagtttg 
      aaaaaactc aaagaactc atcgacttg ttatacgct 
      gaagtgtgg aaacgacgc agaaaaggc tgcaaaaac 
      tcgctgaaa aggggatta aaatcgata gtttatcca 
      tgttga
    mRNA sequence
    • >TC013881 RA
      tagatcaaa tgaacatca cacggtttt atttattta 
      taccacagt ttatacgta tttctgaaa caaattaca 
      ggcaaattt ataattaag ttgtttttt gccaacaat 
      tgttacatt gcgagcaaa caaggtgaa gaagtgatg 
      attttggaa ttttttgag gttatggtc ccttaactt 
      caagaaaaa ttgtcgccc cttaatgaa aatggctag 
      aaatttgtg tcagttttt gaaaatcga tgttcgact 
      attgcttcc ttcactgca tttaaatgc gaggttata 
      ttctgcaag tcctgttaa aaaatcgga aaatgtggc 
      tccggttta caccaagag agacgatga gtataaaat 
      gaccaagaa aggggctgt tagtacttt gcagaagct 
      gttgcgact taaaacttt tgacgcgtt taacattgt 
      gttgaataa tgatttttt caacaatgt gtctaaagc 
      aactttaaa aggcaatta tgcacttct tgtgcagaa 
      aggaataac accagaaac tattttgaa gtatccaga 
      attgttaac agtgtcggg aaccgagca gaaactaca 
      gattataga ggcgctaaa atatgatat aaatgaagt 
      tgcaattct tttattact aaacatacc tagtcttaa 
      aaaattaga agagaatga ggtggatcc agaagggag 
      cactataac gagtctttc ttcactttt aaatataga 
      ccatcagaa agcgtgtga atatttggc acgaaaacc 
      ttttctgtg ttttctttc gaacgataa aatcacgag 
      aaaaataga aatactaaa aagttatgg catcccatt 
      agaagaaat ctgcaacga cttgtgggt tttaagata 
      cagagaaaa attaataga ggaacgttt aagtcgagc 
      aaagaaata caacatcgg agctgcaaa aacttggat 
      ggtgcgagc caaacaaga agtgtttca agtctatat 
      ccaaagacg ttctgacaa taagtcgat tctgggcaa 
      cagttccct cgccgagta cttatcaga acgcctcaa 
      atgcagcgt agaagtggc aaattacat gataattaa 
      acagccagc cctccaaaa caaaagttt gaaaaaact 
      caaagaact catcgactt gttatacgc tgaagtgtg 
      gaaacgacg cagaaaagg ctgcaaaaa ctcgctgaa 
      aaggggatt aaaatcgat agtttatcc atgttgact 
      aaaagccaa aaccaattc atgcagtac tacgaggct 
      ttgctgaag agtaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY