Status
Please wait ...
Gene information
General information
TC003232 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 123729..124780
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003232
    iBeetle screen iB_10658

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003232 RA
      MAPAYKLTY FEYTGLAEV SRFLMKFGG 
      IDFEDCRIK MEEWPQLKA KFPFGQVPV 
      LEYKGKIAC QSLAIARYL AKQVKLSGN 
      DDWENLEID ATIDTINDL RMKLATWWF 
      EADEAKKKL IVENFKKDN LSYYLPRLE 
      AIVKKNKGF LAVGRLTWA DFYWATISP 
      VFDMVTGID ALADYPELK AARERVNAL 
      PAVKKWIDE RPNN
    CDS sequence
    • >TC003232 RA
      atggcacca gcttacaaa ttgacttat ttcgagtac 
      acaggcctt gccgaagtc tcccgtttc cttatgaaa 
      ttcggtgga attgatttt gaagactgt cggatcaaa 
      atggaagag tggcctcaa ctgaaagcc aaatttcca 
      ttcggccaa gtgcctgtg ttggagtat aagggaaaa 
      attgcgtgt caaagcctt gcaattgca cgatattta 
      gccaaacaa gtaaaattg agtggaaac gatgattgg 
      gaaaatctc gaaatcgat gctaccatt gacaccatc 
      aacgatctt cgcatgaag cttgcaact tggtggttt 
      gaggcagac gaggcgaaa aagaagctc attgttgag 
      aatttcaaa aaagacaat ttgtcctac tatttaccc 
      cgtttagag gcaatcgtt aaaaagaat aaaggcttt 
      ttggcagtt ggacgactc acttgggcc gatttttac 
      tgggcgaca atttcacct gtttttgat atggtcaca 
      gggattgac gcacttgct gattatcca gaattaaaa 
      gctgccaga gaaagagtt aacgcttta ccagctgtc 
      aagaaatgg attgatgag cgacccaac aactga
    mRNA sequence
    • >TC003232 RA
      aacaaatag cagattgcg ctcaatggt gcaactttt 
      gatcagtta tttttgaaa caccgaaag tgtttgtct 
      aaaaaatgg caccagctt acaaattga cttatttcg 
      agtacacag gccttgccg aagtctccc gtttcctta 
      tgaaattcg gtggaattg attttgaag actgtcgga 
      tcaaaatgg aagagtggc ctcaactga aagccaaat 
      ttccattcg gccaagtgc ctgtgttgg agtataagg 
      gaaaaattg cgtgtcaaa gccttgcaa ttgcacgat 
      atttagcca aacaagtaa aattgagtg gaaacgatg 
      attgggaaa atctcgaaa tcgatgcta ccattgaca 
      ccatcaacg atcttcgca tgaagcttg caacttggt 
      ggtttgagg cagacgagg cgaaaaaga agctcattg 
      ttgagaatt tcaaaaaag acaatttgt cctactatt 
      taccccgtt tagaggcaa tcgttaaaa agaataaag 
      gctttttgg cagttggac gactcactt gggccgatt 
      tttactggg cgacaattt cacctgttt ttgatatgg 
      tcacaggga ttgacgcac ttgctgatt atccagaat 
      taaaagctg ccagagaaa gagttaacg ctttaccag 
      ctgtcaaga aatggattg atgagcgac ccaacaact 
      gatttaata aatcataat tccttataa agttctcca 
      ttctgttgt tcaaacaga ctcaaaaat aaatacact 
      actagtaac tgattcgtc cattaaatt caacgctgt 
      ttcatttta gaaaatttg ttgtttttg tgattgttt 
      taaataaga cgtattaca cgtattgtt tacccctgt 
      aaaatgcaa ccaccaata cacagtata tcagtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY