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Gene information
General information
TC013430 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9740855..9744248
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013430
    iBeetle screen iB_10653

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs femur random potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs tibiotarsus random potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen cuticle shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    posterior terminus structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013430 RA
      MADSASESD SSSIDGGPI MMPPSPITR EQLQKRIES 
      LQQQNRVLK VELETYKLR VKALQEENR GLRQASVII 
      QAKAEQEEE FISNTLLKK IQALKKEKE SLAHHYERE 
      EECLTNDLS RKLTQLRQE KCRLEQTLE QEQECLVNR 
      LMRKIEKLE AETLAKQSN LEQLRREKV ELENTLEQE 
      QEALVNKLW KRMDKLEAE KRMLQIKLD QPVSDPASP 
      RDINNGDTA TNLSAHIQT LRSEVARLR QTLAISQQE 
      HTQKMQRYA QEERNIKEE NLRLQRKLQ LEVERREAL 
      CRHLSESES SLEMEEERH YNEQVLGAR QHTVSPVPY 
      NPSPSQSRP LSPGLSSMQ APSPRDSLI VGPPRCPTC 
      GQLLTAACV PALHNVGSS SPPAPAHRR TSERFIKPA 
      IPAPQPASP MDTSITKE
    CDS sequence
    • >TC013430 RA
      atggctgat tcagcgagt gagtctgat tcgagcagc 
      attgacggg ggccccatc atgatgccc cccagcccc 
      ataaccagg gagcagctc caaaagcgg atagaaagc 
      ttgcagcag caaaatcgg gtgcttaaa gtcgaactc 
      gagacctac aagctgcgg gtcaaggcg ctccaggag 
      gaaaatcgc ggcctgaga caggcctct gtcattatc 
      caagccaag gctgagcaa gaggaggag tttatttca 
      aacactctt cttaagaag attcaagcg ttgaagaag 
      gaaaaggag tctttagcc caccactat gaaagggag 
      gaggagtgt cttaccaac gatctctcg cggaaactc 
      acgcagctg cgacaggag aagtgccga cttgaacaa 
      actctagag caggaacaa gagtgcctt gttaaccgt 
      ctaatgaga aaaattgag aaactagaa gctgagacg 
      ctggccaag cagagcaac ttggagcag ctgagacgc 
      gaaaaagtc gagcttgag aacactctt gagcaggag 
      caagaagct ctcgttaac aagctctgg aagcgcatg 
      gacaagctc gaggccgag aagcgaatg cttcagatc 
      aaattggac caaccggtc tcagatccg gcgagtcct 
      cgtgacatc aacaacggc gatactgcc accaatctc 
      agtgcccac atccagact ctccgttcg gaagtggcc 
      cgtttgagg caaacatta gcaatcagc caacaagag 
      cacacccag aagatgcaa agatacgcg caagaggaa 
      cgcaacatc aaggaggag aacttgagg ttgcagagg 
      aagctgcag ttggaggtg gagaggcgc gaagctctc 
      tgtcgccac ctctcggag agcgaaagc tcgctggag 
      atggaggag gagaggcat tacaacgag caagtgctt 
      ggggccagg cagcacacg gtgtcgccg gttccgtac 
      aatccgtcg cctagccag agtcgcccc ctgagtcca 
      ggtttgtcg tcgatgcaa gccccatcc ccgcgcgac 
      tcgctaatc gtgggcccc ccacgctgt ccgacttgc 
      ggtcaatta ctaacggcg gcttgcgtg cctgctttg 
      cacaacgtg ggttccagc agccccccg gccccggcc 
      caccgaagg accagcgaa agattcatc aagccggcg 
      attccagca ccacagccc gctagtccc atggatact 
      tcaatcact aaagaataa
    mRNA sequence
    • >TC013430 RA
      aaaataaaa gtcctaaat tgaaaattt ttgtgcaat 
      tttttactt tactatact ttccgaaac gtgtaatat 
      tgccttgtt gttcaattt gtttgcgat aaggtaaag 
      ttcatccga tttgaaggc gtattacct tcgataatt 
      cgttctcca gccggtttt ttggtaaca ttttccgca 
      ttggcagca atgtgcatg gcggctgtc aagaaacga 
      aaccaaaaa ttgtcatct gtcctcatg tttattgct 
      aattgttta attttatag ttacagtga agcaataaa 
      gacatggct gattcagcg agtgagtct gattcgagc 
      agcattgac gggggcccc atcatgatg ccccccagc 
      cccataacc agggagcag ctccaaaag cggatagaa 
      agcttgcag cagcaaaat cgggtgctt aaagtcgaa 
      ctcgagacc tacaagctg cgggtcaag gcgctccag 
      gaggaaaat cgcggcctg agacaggcc tctgtcatt 
      atccaagcc aaggctgag caagaggag gagtttatt 
      tcaaacact cttcttaag aagattcaa gcgttgaag 
      aaggaaaag gagtcttta gcccaccac tatgaaagg 
      gaggaggag tgtcttacc aacgatctc tcgcggaaa 
      ctcacgcag ctgcgacag gagaagtgc cgacttgaa 
      caaactcta gagcaggaa caagagtgc cttgttaac 
      cgtctaatg agaaaaatt gagaaacta gaagctgag 
      acgctggcc aagcagagc aacttggag cagctgaga 
      cgcgaaaaa gtcgagctt gagaacact cttgagcag 
      gagcaagaa gctctcgtt aacaagctc tggaagcgc 
      atggacaag ctcgaggcc gagaagcga atgcttcag 
      atcaaattg gaccaaccg gtctcagat ccggcgagt 
      cctcgtgac atcaacaac ggcgatact gccaccaat 
      ctcagtgcc cacatccag actctccgt tcggaagtg 
      gcccgtttg aggcaaaca ttagcaatc agccaacaa 
      gagcacacc cagaagatg caaagatac gcgcaagag 
      gaacgcaac atcaaggag gagaacttg aggttgcag 
      aggaagctg cagttggag gtggagagg cgcgaagct 
      ctctgtcgc cacctctcg gagagcgaa agctcgctg 
      gagatggag gaggagagg cattacaac gagcaagtg 
      cttggggcc aggcagcac acggtgtcg ccggttccg 
      tacaatccg tcgcctagc cagagtcgc cccctgagt 
      ccaggtttg tcgtcgatg caagcccca tccccgcgc 
      gactcgcta atcgtgggc cccccacgc tgtccgact 
      tgcggtcaa ttactaacg gcggcttgc gtgcctgct 
      ttgcacaac gtgggttcc agcagcccc ccggccccg 
      gcccaccga aggaccagc gaaagattc atcaagccg 
      gcgattcca gcaccacag cccgctagt cccatggat 
      acttcaatc actaaagaa taagataaa aaaggtgtc 
      gagttgaca aataagtaa tcatgttga tgggctttc 
      gttttgcag gtggtgaac atttgttgg ttttctctg 
      catgttatt tgtataggg gctttaaag ttcgaaaca 
      aataaattt aataaaact acatggtgt tactgtgaa 
      actaatgga tacataatt ttttcttat ttagatata 
      ttatacttg gatttttcg attttcggg cagctgttt 
      ttgattaat ttcgggatc tgctttgct tttaatatg 
      tgtacttaa tgatgttat atgataaag ggagcttca 
      ttgtggtca gctctacta aaatatttc catatttga 
      atgtttaca agtgatctt tcaatctag caacggcag 
      ttgtcacag gtccgatgc catgttgaa ccattgcca 
      acaattttc acaatgttt gtgatatgt gagtttttg 
      ttaattaaa attgtgaca cgatttctt aacaatttc 
      gtttagttt gtacactgc actctgcca aacttgttt 
      tcttagtat tcattattt tgtcatgca ataataaat 
      ctagcttac gttttaagt aatggatat cacgttttt 
      tatttgtaa caccatttc aattaagta agtttaatt 
      gaaatgttg aaggttatt tatccgatt tgttacatt 
      cataacact agaaattac gaattaaag actatttag 
      tttagagtt aggtgtttt ttagattaa catctcaaa 
      aattgacac tacaggtgg caattcaaa aaaattgtg 
      aaaagtgat attaggata taaactcat tagtttcat 
      tggtggaat tacattatc atcttgatt tgccaaatt 
      tctaaattt aaacaaaat tcttccctt actaaatta 
      aaagccaat gaaaacttt cggtgacta tttcagggt 
      catatgtta gtctaggat aacattttg ttaatgtga 
      gtttgagat atttgttat gtttttagt aaactgatg 
      tattttcct gaaaatgtt gtttttatc ttgtttttt 
      ttgtaaaaa aagctattc tatttagta aacgctgtg 
      aatcataaa aaagattac attggccta ttgcaccaa 
      cagtcccag aaacaaatg attctgaat aaacaactg 
      gcttacgaa tttcctgta atctttacg ctacgaatt 
      attttctat tttaataat ttgtataag gttgaatga 
      tatgtaatg gtttttaaa taataataa acacttaaa 
      agcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY