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Gene information
General information
TC012874 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15275224..15277812
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012874
    iBeetle screen iB_10649

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012874 RA
      MSLKLMSAV ISPNIANQP KSETADKLL RQLLECVQE 
      CQKRYGGKT ELATEFDSC VVALCLTLE AVLLHGIRV 
      NPQGSALKQ VASSLHLGG ENPSFWPFV RTHLTKHEQ 
      ERYAVLKHI WTDTGRGRA WIRAALNER SLERYFHIL 
      LSNQSILEI YYEGCAILR DLEKSSMLP NMAGGVGSI 
      LFAISIDKP ELNSGIPPR IDSIKSKAE PIIEAPLSE 
      RPKTRKKKK VARQFISFD EDDSVLSTS VPSTSSSIS 
      SDTSTSNEQ QQSSTKLKK ILEENRMAE NAKPRRKLS 
      IETSGSREK FREIPETLT PVSHAEIGE LTPVSVEIN 
      KNFESDAVV EVPTDISEV LTAVEARNT EELNKKQER 
      IDQLSKENE ILKEQVKKY VSAIQMLKN NENVTLEDL 
      DINQQPDYQ GEAKLFEKK LVQVAEMHA ELMDFNVAL 
      QQSLCQKDQ LVERLKAEL EQLRGPCHD DDTEGEVSG 
      NVNVWIPSA FLTGTGSSA HHVYQIFLR AGNDEWNIY 
      RRYAQFYAL HSDLKKLDP AVTSFDFPP KKSIGKKDS 
      ALVEERRKR LQVYLRRVL AHWPELSHC NSRFLLEQH 
      LAFFKDQKD GEPNKGIFS PRRSRNTND NHYAGL
    CDS sequence
    • >TC012874 RA
      atgtccctg aagctcatg tcggctgta atttcgcca 
      aatatcgca aatcaaccg aaatccgaa accgctgat 
      aaactccta cgacagtta ctcgagtgc gtgcaagag 
      tgccaaaaa cgatatggg ggaaaaacc gaactagcg 
      actgagttt gatagctgt gttgtggcc ctgtgcttg 
      acgctcgag gccgttttg ctgcacgga atccgggta 
      aacccccaa ggctccgct ttgaagcaa gtggcgagc 
      agtctgcat ctagggggc gagaaccct tcgttttgg 
      ccgtttgtt aggacgcat ttaacgaag catgagcag 
      gagagatac gccgttttg aaacatatc tggactgac 
      acgggccgc gggcgtgcg tggatcagg gcggcgctg 
      aacgagcgt tctctggaa agatatttt catatttta 
      ttgagcaat caatcgata ttagagatt tactatgaa 
      ggctgtgca attttacgc gatttggag aagagttcc 
      atgttgccc aacatggcg gggggcgta ggctcgatc 
      ctcttcgcc attagtata gataaacct gaactaaac 
      tcggggatt cccccccgc atcgacagt atcaaatcg 
      aaggcggag cccatcatt gaggccccc ttgagcgag 
      cgccccaaa acccggaaa aagaaaaag gtggcgcga 
      caatttatc tcatttgat gaggacgat agtgttttg 
      tcaactagc gtcccatca acctctagc agtatttcc 
      tctgataca agtacctcg aatgaacag caacagtct 
      tccactaag ttgaaaaaa attctcgaa gaaaacaga 
      atggccgaa aacgcgaaa ccgcgtcgg aaattatcg 
      atcgaaacg agtggttcc cgcgaaaag ttccgggaa 
      atccccgaa acacttact ccagtctcg cacgccgaa 
      atcggcgaa cttacccca gtttcggtc gaaatcaat 
      aaaaatttc gaatcggac gcggttgtg gaagtcccg 
      actgacatt agcgaagtc ctaacggca gtcgaagcc 
      cgcaatacc gaagaactg aacaagaaa caagaacgc 
      atcgatcag ttgtccaaa gagaacgag attctcaaa 
      gagcaggtt aagaagtac gtcagtgcg attcaaatg 
      ttgaaaaac aacgaaaac gtgacactt gaagatttg 
      gacatcaac cagcaaccg gattatcaa ggcgaggcg 
      aaacttttc gaaaaaaaa ttagtccaa gttgccgaa 
      atgcacgcc gaattgatg gactttaac gtggctttg 
      cagcaatcg ctctgtcag aaggaccag ctggttgag 
      cggctcaag gccgagctg gagcaactc cgggggccg 
      tgtcatgat gatgacacg gagggcgaa gtctcgggg 
      aatgttaat gtctggatt ccttcggcg tttttgacc 
      gggactggg tcaagtgca catcatgtt tatcaaatt 
      tttctaagg gccgggaat gacgagtgg aatatttat 
      aggcgatat gcgcagttt tatgcattg cactctgat 
      ttgaaaaag ttggatccg gcggtcacg tcgtttgat 
      tttccgccg aaaaaaagt atagggaaa aaggattca 
      gcgttagtt gaagagcgc cgaaagcga ctgcaagtc 
      tatctgagg cgcgtttta gcgcattgg cccgaacta 
      tcgcattgc aatagccgg tttttacta gagcaacat 
      ttggctttt ttcaaggac cagaaggac ggtgagccg 
      aataaaggg attttctcg cctcgaaga agccgaaat 
      actaatgat aatcactat gcagggctt taa
    mRNA sequence
    • >TC012874 RA
      taattttgc ttaaattcg tctaaaaca accaaaatt 
      cggctaaaa tcgccccaa atcagtcgt ttccagcca 
      aaacagccc aattttggc caaattaac tcgctgaca 
      ttagcccag tttgacact tcccaatca gctgattgt 
      accagtagt gtctgtttt gtgttttta atcgaaaat 
      cctgtgata tcacgtttc tggcgataa gggccccac 
      tgcaaccca tcttatcgc aatgttatg ccacctcga 
      gtcagattc acccatgcc atgaccggc caaggttat 
      aattatgtc cctgaagct catgtcggc tgtaatttc 
      gccaaatat cgcaaatca accgaaatc cgaaaccgc 
      tgataaact cctacgaca gttactcga gtgcgtgca 
      agagtgcca aaaacgata tgggggaaa aaccgaact 
      agcgactga gtttgatag ctgtgttgt ggccctgtg 
      cttgacgct cgaggccgt tttgctgca cggaatccg 
      ggtaaaccc ccaaggctc cgctttgaa gcaagtggc 
      gagcagtct gcatctagg gggcgagaa cccttcgtt 
      ttggccgtt tgttaggac gcatttaac gaagcatga 
      gcaggagag atacgccgt tttgaaaca tatctggac 
      tgacacggg ccgcgggcg tgcgtggat cagggcggc 
      gctgaacga gcgttctct ggaaagata ttttcatat 
      tttattgag caatcaatc gatattaga gatttacta 
      tgaaggctg tgcaatttt acgcgattt ggagaagag 
      ttccatgtt gcccaacat ggcgggggg cgtaggctc 
      gatcctctt cgccattag tatagataa acctgaact 
      aaactcggg gattccccc ccgcatcga cagtatcaa 
      atcgaaggc ggagcccat cattgaggc ccccttgag 
      cgagcgccc caaaacccg gaaaaagaa aaaggtggc 
      gcgacaatt tatctcatt tgatgagga cgatagtgt 
      tttgtcaac tagcgtccc atcaacctc tagcagtat 
      ttcctctga tacaagtac ctcgaatga acagcaaca 
      gtcttccac taagttgaa aaaaattct cgaagaaaa 
      cagaatggc cgaaaacgc gaaaccgcg tcggaaatt 
      atcgatcga aacgagtgg ttcccgcga aaagttccg 
      ggaaatccc cgaaacact tactccagt ctcgcacgc 
      cgaaatcgg cgaacttac cccagtttc ggtcgaaat 
      caataaaaa tttcgaatc ggacgcggt tgtggaagt 
      cccgactga cattagcga agtcctaac ggcagtcga 
      agcccgcaa taccgaaga actgaacaa gaaacaaga 
      acgcatcga tcagttgtc caaagagaa cgagattct 
      caaagagca ggttaagaa gtacgtcag tgcgattca 
      aatgttgaa aaacaacga aaacgtgac acttgaaga 
      tttggacat caaccagca accggatta tcaaggcga 
      ggcgaaact tttcgaaaa aaaattagt ccaagttgc 
      cgaaatgca cgccgaatt gatggactt taacgtggc 
      tttgcagca atcgctctg tcagaagga ccagctggt 
      tgagcggct caaggccga gctggagca actccgggg 
      gccgtgtca tgatgatga cacggaggg cgaagtctc 
      ggggaatgt taatgtctg gattccttc ggcgttttt 
      gaccgggac tgggtcaag tgcacatca tgtttatca 
      aatttttct aagggccgg gaatgacga gtggaatat 
      ttataggcg atatgcgca gttttatgc attgcactc 
      tgatttgaa aaagttgga tccggcggt cacgtcgtt 
      tgattttcc gccgaaaaa aagtatagg gaaaaagga 
      ttcagcgtt agttgaaga gcgccgaaa gcgactgca 
      agtctatct gaggcgcgt tttagcgca ttggcccga 
      actatcgca ttgcaatag ccggttttt actagagca 
      acatttggc ttttttcaa ggaccagaa ggacggtga 
      gccgaataa agggatttt ctcgcctcg aagaagccg 
      aaatactaa tgataatca ctatgcagg gctttaaac 
      tagatctgt ggaggtttc cagtaataa attagggtt 
      atttgttac gaataatac gttttatat tttatgatg 
      agcagtaaa gataaataa aataatttg aataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY