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Gene information
General information
TC031431 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450689.1: 89322..90569
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031431
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10647

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031431 RA
      MYNFLDPKL SPKVSSSAP ATDNYEASN LISENGAVR 
      ARGFLAYTV TRPPVALEF EFLCPISVS FIALETSVG 
      RQKCTGIEL FAKNSTTPF TSIARAVFD KSGLTVCDS 
      KTYNKSKPP PCHTPDKEL RYFKSDQRR LWGDTTCLK 
      VVIFRTDRS VPCLGAIKV FGAPSGSCS KVTVETIGR 
      IMGGSPEAP QTGGGQEGL KIPQDFVDD LTCEIMAIP 
      MTLPSGKTV DRDTLDKHI ESEKSFGRK PCDPFTGLK 
      FTETRKPVI NVALKSRID MFLMQNSTN PAFFGLKRA 
      LGGQNLRAD NKRAKYGGE KECCSCRCC QFLYKIPCQ 
      HYYCRNCLL QVVKLECNS CGLEFVKSD AVRCH
    CDS sequence
    • >TC031431 RA
      atgtacaac ttcctggac cccaaactg agccccaaa 
      gtgtccagc agcgccccc gccaccgac aattacgaa 
      gcctccaac ctaatcagc gaaaacggg gctgtccga 
      gcccgcggt ttcctagct tacactgta acgcgcccc 
      cctgtagcc ctagaattc gagtttttg tgccccatt 
      agtgtctcg ttcattgca cttgagaca agtgttggg 
      cgccaaaag tgtacagga atcgaactt tttgcaaaa 
      aactccact actcccttt acttcaatc gccagagcg 
      gtctttgac aagtcgggt ttaaccgtc tgtgatagc 
      aaaacttat aataaatct aaaccgccc ccttgtcac 
      actcccgat aaagaactg cgttatttt aaaagtgat 
      cagaggcgc ctttggggc gacaccacc tgcctcaaa 
      gttgtgatt tttcgcacc gatcgcagc gtgccctgc 
      ttgggggcg attaaggtg ttcggcgcc ccttcgggg 
      agttgctcc aaagtgact gtcgagaca attgggagg 
      attatgggg ggttccccg gaagcgccc caaacgggg 
      ggaggacaa gaggggtta aaaatcccc caggatttt 
      gttgatgac ttaacttgc gaaattatg gcaattccc 
      atgactcta ccaagtggc aaaactgtg gatagagac 
      acgcttgac aaacacata gagagtgaa aaatcgttc 
      ggtcgtaaa ccttgcgat cccttcaca ggacttaaa 
      ttcaccgaa acgcgcaaa ccggtgatc aatgttgcg 
      ctcaaaagc agaattgat atgtttttg atgcagaat 
      tcgacaaat ccggcgttt tttgggcta aaaagggcg 
      ctagggggg caaaatttg agggccgat aataagagg 
      gccaagtac gggggggag aaagagtgt tgtagttgt 
      cggtgttgc cagttttta tacaaaatt ccctgccag 
      cattattat tgcaggaat tgtctgtta caagttgta 
      aaattagag tgtaattct tgtggactc gaatttgta 
      aaaagtgac gctgtacgt tgtcattaa
    mRNA sequence
    • >TC031431 RA
      aaccaagtt aatttaatt tacacaaat ttaaagtcc 
      acggtgata ttttcgcgc ttagtttag tatgtacaa 
      cttcctgga ccccaaact gagccccaa agtgtccag 
      cagcgcccc cgccaccga caattacga agcctccaa 
      cctaatcag cgaaaacgg ggctgtccg agcccgcgg 
      tttcctagc ttacactgt aacgcgccc ccctgtagc 
      cctagaatt cgagttttt gtgccccat tagtgtctc 
      gttcattgc acttgagac aagtgttgg gcgccaaaa 
      gtgtacagg aatcgaact ttttgcaaa aaactccac 
      tactccctt tacttcaat cgccagagc ggtctttga 
      caagtcggg tttaaccgt ctgtgatag caaaactta 
      taataaatc taaaccgcc cccttgtca cactcccga 
      taaagaact gcgttattt taaaagtga tcagaggcg 
      cctttgggg cgacaccac ctgcctcaa agttgtgat 
      ttttcgcac cgatcgcag cgtgccctg cttgggggc 
      gattaaggt gttcggcgc cccttcggg gagttgctc 
      caaagtgac tgtcgagac aattgggag gattatggg 
      gggttcccc ggaagcgcc ccaaacggg gggaggaca 
      agaggggtt aaaaatccc ccaggattt tgttgatga 
      cttaacttg cgaaattat ggcaattcc catgactct 
      accaagtgg caaaactgt ggatagaga cacgcttga 
      caaacacat agagagtga aaaatcgtt cggtcgtaa 
      accttgcga tcccttcac aggacttaa attcaccga 
      aacgcgcaa accggtgat caatgttgc gctcaaaag 
      cagaattga tatgttttt gatgcagaa ttcgacaaa 
      tccggcgtt ttttgggct aaaaagggc gctaggggg 
      gcaaaattt gagggccga taataagag ggccaagta 
      cggggggga gaaagagtg ttgtagttg tcggtgttg 
      ccagttttt atacaaaat tccctgcca gcattatta 
      ttgcaggaa ttgtctgtt acaagttgt aaaattaga 
      gtgtaattc ttgtggact cgaatttgt aaaaagtga 
      cgctgtacg ttgtcatta ataaatatt tttataaaa 
      acgattttt ttttatttc ctgttcaaa actgttgtt 
      tgcacttac acttcactg ttaaaaaat tcataactc 
      gaaaactaa aagtcgtag agcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY