Status
Please wait ...
Gene information
General information
TC015499 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1554509..1555544
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015499
      iBeetle screen iB_10646

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015499 RA
        MLSTYLNTL PLPKFVLIE DSVAAKGGD FFEHQLRSH 
        ANRSDYATH LLIFESYFT SLKNRHSSC TCHDFTSDS 
        LNWFKKSAD LTSLTSLNE NCVIFVDSL AHVLYRFGA 
        AETYKLFTQ LKNKKNVQQ VVTVLHTDL LENKDKIVK 
        YFRHLATLY IELDTKRLS YLYKKSHRK VIKQIENYH 
        FDNKRLITE TIKKPDKEI IAETLNRLP ENLSTFRIG 
        LTEKEKESR DGLVLPFLP KESESSGGK IHYSLDEID 
        DWDEEDPDD DLDI
      CDS sequence
      • >TC015499 RA
        atgttgtcc acttattta aacaccttg ccgttgccc 
        aaattcgtc ctaattgaa gattcagtg gcggcaaaa 
        gggggcgat tttttcgaa caccagctc agaagtcac 
        gccaatagg agtgactac gccactcat ttgctcatt 
        tttgagagt tatttcact agtttgaaa aacagacac 
        agttcttgc acttgtcat gactttacc agtgattcc 
        ctcaactgg tttaaaaaa agtgcagat ttgacgagt 
        cttacaagt ttgaatgag aattgtgtg atttttgtt 
        gattccctg gctcatgtt ttgtaccgt tttggggcc 
        gctgagacg tacaaatta ttcacacaa ttgaaaaat 
        aaaaaaaat gtacaacaa gttgttaca gttttacac 
        accgattta ctggaaaat aaagataaa attgttaaa 
        tattttcgc cacttagca acgctttat atagaattg 
        gacacaaaa cgcttatcg tatttgtat aaaaagagt 
        cataggaaa gttataaaa caaatcgag aattatcac 
        tttgataat aagagatta atcaccgaa acgatcaaa 
        aaaccagac aaggaaata atcgcagag actttaaat 
        cgccttccg gagaacttg tccactttt aggataggt 
        ttgactgag aaggagaaa gaatcaagg gatggttta 
        gttctacca tttttaccc aaggaaagt gaaagttcg 
        ggtggaaaa atacactat tccttggat gagattgac 
        gattgggac gaagaagat ccagacgat gatctcgac 
        atttag
      mRNA sequence
      • >TC015499 RA
        ttttttgtg attgcagca tttacaaat gttgtccac 
        ttatttaaa caccttgcc gttgcccaa attcgtcct 
        aattgaaga ttcagtggc ggcaaaagg gggcgattt 
        tttcgaaca ccagctcag aagtcacgc caataggag 
        tgactacgc cactcattt gctcatttt tgagagtta 
        tttcactag tttgaaaaa cagacacag ttcttgcac 
        ttgtcatga ctttaccag tgattccct caactggtt 
        taaaaaaag tgcagattt gacgagtct tacaagttt 
        gaatgagaa ttgtgtgat ttttgttga ttccctggc 
        tcatgtttt gtaccgttt tggggccgc tgagacgta 
        caaattatt cacacaatt gaaaaataa aaaaaatgt 
        acaacaagt tgttacagt tttacacac cgatttact 
        ggaaaataa agataaaat tgttaaata ttttcgcca 
        cttagcaac gctttatat agaattgga cacaaaacg 
        cttatcgta tttgtataa aaagagtca taggaaagt 
        tataaaaca aatcgagaa ttatcactt tgataataa 
        gagattaat caccgaaac gatcaaaaa accagacaa 
        ggaaataat cgcagagac tttaaatcg ccttccgga 
        gaacttgtc cacttttag gataggttt gactgagaa 
        ggagaaaga atcaaggga tggtttagt tctaccatt 
        tttacccaa ggaaagtga aagttcggg tggaaaaat 
        acactattc cttggatga gattgacga ttgggacga 
        agaagatcc agacgatga tctcgacat ttagttaca 
        tttgtttgg atttatatt gttattaat aaaaagttt 
        ttttacga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY