General information | |||
TC004970 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 5375365..5380044 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC004970 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_10645 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
head remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs structure irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
metathorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
mesothorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
legs sometimes randomstructure not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC004970 RA
MDIFLFMFL VFISFLIGI IVTLVVQYY ILYSYFKTS PVVTPNEKK CSTDYVLPE SLKKQLDSE ELNDATSLS VSLVLQFLF HELRHSEAI KRWLYKKLS LEFEELLTK TTIGKFFEV INIRDMNLG NNFPNIKKI SIEDVKLDN KEGHIDTVS LCLDVDYTG NFLLSVDAK MKFGKTAYL SIKVNKVNG LVRLQFTRH PYTHWSFSF FQDPIIELE VESHFQGRQ LQSNITSLI VNQIKKAIR RKHTLPNYK IRYKPFFIK TDPSQLDTD DSEIVPQGQ LEVTCIDVT RLEFSNAVQ NVYCTVAVD TIPWICVYE SDERTFMIL EITMTKLKQ AQLGVIFKP EKSSVAVES VSPHSCAFK SGLKPGDIV LAVENKSVS SVPQVAKFI KSVTSTHVT LRVKRLVDS YVLRKKHFD KNSTDEMDQ DESSFFIVD SVETPKKPA EKIPKSLSS NENVSKFAQ TIGNFSLRK RKASVSDKS STEVSCKST PTSSSPGTP QHGVFKQHT SPLLAGKKQ SISEIPEIL RENSESEII EVGKSKELG PTSVLYFNE DFQFSLKKG LKYLNVNVW GTVTDDKDV LLGYANIPL SHILNECFN SVLGHYMRR YSFLPPAFC HSNNQSHPL MAHSGFEHV FCYGDILLS FIWSHEDDI EVKRKMSQD ILNTDIEQI STSTALKHD FIRTQFHRT THCDFCSKK IWLKDAVQC RECGLCCHK KCIAKCQTS TACIQGDKK TEPEAVQPE ITMTEPPDE MTEYAEGGL KRVNSVKNL AIPGAHLLS TVSKSLPPS PQRTPSRKQ SMSNINPFA LCPGVLEDV QKKPEEASE SVNRLLEQV MQCPPDETL MDVAKETGR QLYANMPHE EKVEKINIM MAELKKTLD SVTMEHMEL SKQLSSQES DAEKTKLAF LIGQADAKV QGLSVLMLH YCSSLQHTQ EKIV
- >TC004970 RA
atggatata tttttgttc atgttccta gtttttata tctttttta attggtatt atagtgact ctcgtggtg caatattat attttgtat tcttatttt aaaacaagt ccagtggtc acaccaaat gagaaaaag tgttctact gattacgtt ttaccagag tctttgaaa aaacaattg gatagtgaa gaattgaac gatgcgacg agtttatcg gttagcctg gttttacag tttttgttt cacgaattg aggcattct gaggctatt aaacgatgg ctttataag aagctgagt cttgagttt gaggaacta ctaactaaa actaccata gggaaattt ttcgaagtc attaatatt agggatatg aacttaggt aacaacttt ccaaatatt aaaaaaatc agtattgaa gatgtgaaa ttagataat aaggaagga cacatagac actgtcagt ttatgtcta gatgtagat tatacagga aacttttta cttagtgtt gatgccaaa atgaaattt ggaaaaact gcatattta tcaattaag gttaataaa gtcaacgga cttgtaaga cttcagttt acaagacat ccgtacaca cactggtct ttcagcttt tttcaagac cccattata gaattagaa gttgaatca cactttcag ggacgtcaa ttacagtca aacatcaca agtcttatc gtaaaccaa ataaaaaaa gccatcaga cgaaaacac acactacca aattacaaa atcagatac aaaccgttc tttatcaaa actgaccct agccaacta gacacggac gatagcgaa atcgtccct cagggccaa cttgaagtc acatgcata gatgtcaca cgacttgag ttctcaaat gcggttcaa aacgtttat tgcactgtt gcagttgac acgattcct tggatttgt gtttatgaa tcagatgag cgcactttc atgattcta gaaatcaca atgactaaa ttaaaacag gcccaactt ggagtgatt ttcaagccg gaaaaaagc tcagtggct gttgaaagc gtctctcca cacagttgc gccttcaaa tctggcctc aaacccggc gatattgtt ttagcagtt gagaataaa tcggtttcg agcgttccc caagtcgcc aaatttatc aaatcagtg acttcgact catgtcact cttcgagtg aaaagactg gtcgattcg tacgttttg cgcaagaaa cacttcgat aaaaactca actgatgag atggaccaa gacgaaagc agcttcttc attgttgat agcgtggaa acgccgaaa aaaccggcg gagaaaatc ccgaaaagt ctttcaagc aacgaaaac gtctcgaaa tttgctcaa acgatcggt aattttagt ttgcgcaaa cggaaggct tccgtttcg gataaatcg tcaacggaa gtgagttgt aaaagtaca ccgacgtct agtagccct ggaaccccg caacatgga gtgtttaaa caacataca agcccgcta ttggctggg aaaaaacaa tcgatctcg gaaataccc gaaattttg cgcgaaaat tccgaaagt gaaattatc gaggtcggg aaaagcaaa gagttgggc ccgacttcg gtgctttat ttcaatgaa gactttcaa tttagcctc aaaaagggg ctcaagtac ttgaatgtc aacgtttgg ggtacagtg actgatgat aaagatgtt ttgttggga tatgcgaat attcccttg agccatatt ttgaacgaa tgttttaat agcgttcta gggcactac atgagacgg tactctttt ctaccgccg gcgttttgc cattctaat aaccaatca cacccactc atggcacac tctggcttc gaacacgtc ttttgctac ggagatatt cttttgtct ttcatttgg tcgcatgag gacgatatc gaagttaag cgtaaaatg tcgcaagat attttaaac acagacatt gaacaaatt tcaacttca acagctctc aagcacgac ttcatcaga acgcagttt caccgaaca actcactgc gatttctgc tcgaaaaag atttggctc aaagacgcg gtccagtgc cgcgaatgc ggtctctgt tgccacaaa aaatgcata gccaagtgt caaacgagc actgcatgc atccaaggt gacaaaaaa accgaaccg gaagcggtc caaccggaa ataaccatg acggaacca cccgacgaa atgaccgaa tatgcagaa ggggggttg aaacgagta aacagtgtg aaaaattta gcaatacca ggtgcgcat ttattgtca actgtttcg aaaagtttg cctccatcg ccccaacga acgccaagt cgcaaacaa tccatgtct aatataaat ccattcgct ttgtgtcct ggtgtttta gaagacgtt cagaaaaaa ccggaagaa gcctctgaa agtgtgaac cgacttttg gaacaagtc atgcaatgc ccccctgat gagacgctc atggatgtg gctaaagaa acgggaagg caactctat gccaatatg ccacatgag gagaaagtt gaaaaaatc aacattatg atggcagag ctgaagaag acgctagat tcagtgacc atggagcat atggaactg tcgaaacag ctaagtagc caagaatcg gacgctgag aagacgaaa ctggcgttt ctgattggt caagccgat gctaaggtt caaggatta tcagtattg atgcttcat tattgttcc agtttacag catactcaa gaaaaaatt gtgtag
- >TC004970 RA
tcttgagaa aaaaacaga aaaaggaaa atcatgttt ggctttttt tgacgctta tcatatatg agataagca caagggggc tataaaatg gtaaactga ctccacttt gtgttattt cgaacaaaa cgggcctaa aaatggtga taatataat ttaaagcgc tatggatat atttttgtt catgttcct agtttttat atctttttt aattggtat tatagtgac tctcgtggt gcaatatta tattttgta ttcttattt taaaacaag tccagtggt cacaccaaa tgagaaaaa gtgttctac tgattacgt tttaccaga gtctttgaa aaaacaatt ggatagtga agaattgaa cgatgcgac gagtttatc ggttagcct ggttttaca gtttttgtt tcacgaatt gaggcattc tgaggctat taaacgatg gctttataa gaagctgag tcttgagtt tgaggaact actaactaa aactaccat agggaaatt tttcgaagt cattaatat tagggatat gaacttagg taacaactt tccaaatat taaaaaaat cagtattga agatgtgaa attagataa taaggaagg acacataga cactgtcag tttatgtct agatgtaga ttatacagg aaacttttt acttagtgt tgatgccaa aatgaaatt tggaaaaac tgcatattt atcaattaa ggttaataa agtcaacgg acttgtaag acttcagtt tacaagaca tccgtacac acactggtc tttcagctt ttttcaaga ccccattat agaattaga agttgaatc acactttca gggacgtca attacagtc aaacatcac aagtcttat cgtaaacca aataaaaaa agccatcag acgaaaaca cacactacc aaattacaa aatcagata caaaccgtt ctttatcaa aactgaccc tagccaact agacacgga cgatagcga aatcgtccc tcagggcca acttgaagt cacatgcat agatgtcac acgacttga gttctcaaa tgcggttca aaacgttta ttgcactgt tgcagttga cacgattcc ttggatttg tgtttatga atcagatga gcgcacttt catgattct agaaatcac aatgactaa attaaaaca ggcccaact tggagtgat tttcaagcc ggaaaaaag ctcagtggc tgttgaaag cgtctctcc acacagttg cgccttcaa atctggcct caaacccgg cgatattgt tttagcagt tgagaataa atcggtttc gagcgttcc ccaagtcgc caaatttat caaatcagt gacttcgac tcatgtcac tcttcgagt gaaaagact ggtcgattc gtacgtttt gcgcaagaa acacttcga taaaaactc aactgatga gatggacca agacgaaag cagcttctt cattgttga tagcgtgga aacgccgaa aaaaccggc ggagaaaat cccgaaaag tctttcaag caacgaaaa cgtctcgaa atttgctca aacgatcgg taattttag tttgcgcaa acggaaggc ttccgtttc ggataaatc gtcaacgga agtgagttg taaaagtac accgacgtc tagtagccc tggaacccc gcaacatgg agtgtttaa acaacatac aagcccgct attggctgg gaaaaaaca atcgatctc ggaaatacc cgaaatttt gcgcgaaaa ttccgaaag tgaaattat cgaggtcgg gaaaagcaa agagttggg cccgacttc ggtgcttta tttcaatga agactttca atttagcct caaaaaggg gctcaagta cttgaatgt caacgtttg gggtacagt gactgatga taaagatgt tttgttggg atatgcgaa tattccctt gagccatat tttgaacga atgttttaa tagcgttct agggcacta catgagacg gtactcttt tctaccgcc ggcgttttg ccattctaa taaccaatc acacccact catggcaca ctctggctt cgaacacgt cttttgcta cggagatat tcttttgtc tttcatttg gtcgcatga ggacgatat cgaagttaa gcgtaaaat gtcgcaaga tattttaaa cacagacat tgaacaaat ttcaacttc aacagctct caagcacga cttcatcag aacgcagtt tcaccgaac aactcactg cgatttctg ctcgaaaaa gatttggct caaagacgc ggtccagtg ccgcgaatg cggtctctg ttgccacaa aaaatgcat agccaagtg tcaaacgag cactgcatg catccaagg tgacaaaaa aaccgaacc ggaagcggt ccaaccgga aataaccat gacggaacc acccgacga aatgaccga atatgcaga aggggggtt gaaacgagt aaacagtgt gaaaaattt agcaatacc aggtgcgca tttattgtc aactgtttc gaaaagttt gcctccatc gccccaacg aacgccaag tcgcaaaca atccatgtc taatataaa tccattcgc tttgtgtcc tggtgtttt agaagacgt tcagaaaaa accggaaga agcctctga aagtgtgaa ccgactttt ggaacaagt catgcaatg cccccctga tgagacgct catggatgt ggctaaaga aacgggaag gcaactcta tgccaatat gccacatga ggagaaagt tgaaaaaat caacattat gatggcaga gctgaagaa gacgctaga ttcagtgac catggagca tatggaact gtcgaaaca gctaagtag ccaagaatc ggacgctga gaagacgaa actggcgtt tctgattgg tcaagccga tgctaaggt tcaaggatt atcagtatt gatgcttca ttattgttc cagtttaca gcatactca agaaaaaat tgtgtaggc gctttttgt ctactaatt aaatttctt gaaacaaaa aagcatatc atcgtgacc ccggtactt aatttttgc atttatgac actcctcta gtcaattaa ttttcacgt attgtagca tttttagtt tttacacgt ttttaggaa cattaacta tacttactg tgatagcgt aggatgtag taagataat ttttaatat ttatagtga ctagatgta tgtgcaaaa ttgtctcta attattggt tataatccc ggcaaaaag agtgaaata aagctttaa cattgttgt attttttgc gtccatgtc agtatttta gttttttat ttttttggc aacttggcc caacgttaa tttgtgaac aaattcttt atgtaatat attttgtat agtaaattt tgcctgctt cacataatg gtcttttat ttcaaacaa ttacgaatg aaactatgg tttaatagg taataaata aataaaggt aataaaggt acctccata cctttatta aaaaattaa aaaagttta gacttgcgt tttcttgga gtcatttct gaaatttat cttctgtga tatagcaga aaatttaaa cattttttt gacggtact tgctatttt aaaattata gaaaaatgg caacagcgc acttacatg ttttggggt caaataggt cacaaataa aaatttatg tgaaattag tgacaaatt tagtgttaa gaaaactcc ctagttaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
