Status
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Gene information
General information
TC004970 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5375365..5380044
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004970
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10645

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs structure irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    metathorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    mesothorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs sometimes randomstructure not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004970 RA
      MDIFLFMFL VFISFLIGI IVTLVVQYY ILYSYFKTS 
      PVVTPNEKK CSTDYVLPE SLKKQLDSE ELNDATSLS 
      VSLVLQFLF HELRHSEAI KRWLYKKLS LEFEELLTK 
      TTIGKFFEV INIRDMNLG NNFPNIKKI SIEDVKLDN 
      KEGHIDTVS LCLDVDYTG NFLLSVDAK MKFGKTAYL 
      SIKVNKVNG LVRLQFTRH PYTHWSFSF FQDPIIELE 
      VESHFQGRQ LQSNITSLI VNQIKKAIR RKHTLPNYK 
      IRYKPFFIK TDPSQLDTD DSEIVPQGQ LEVTCIDVT 
      RLEFSNAVQ NVYCTVAVD TIPWICVYE SDERTFMIL 
      EITMTKLKQ AQLGVIFKP EKSSVAVES VSPHSCAFK 
      SGLKPGDIV LAVENKSVS SVPQVAKFI KSVTSTHVT 
      LRVKRLVDS YVLRKKHFD KNSTDEMDQ DESSFFIVD 
      SVETPKKPA EKIPKSLSS NENVSKFAQ TIGNFSLRK 
      RKASVSDKS STEVSCKST PTSSSPGTP QHGVFKQHT 
      SPLLAGKKQ SISEIPEIL RENSESEII EVGKSKELG 
      PTSVLYFNE DFQFSLKKG LKYLNVNVW GTVTDDKDV 
      LLGYANIPL SHILNECFN SVLGHYMRR YSFLPPAFC 
      HSNNQSHPL MAHSGFEHV FCYGDILLS FIWSHEDDI 
      EVKRKMSQD ILNTDIEQI STSTALKHD FIRTQFHRT 
      THCDFCSKK IWLKDAVQC RECGLCCHK KCIAKCQTS 
      TACIQGDKK TEPEAVQPE ITMTEPPDE MTEYAEGGL 
      KRVNSVKNL AIPGAHLLS TVSKSLPPS PQRTPSRKQ 
      SMSNINPFA LCPGVLEDV QKKPEEASE SVNRLLEQV 
      MQCPPDETL MDVAKETGR QLYANMPHE EKVEKINIM 
      MAELKKTLD SVTMEHMEL SKQLSSQES DAEKTKLAF 
      LIGQADAKV QGLSVLMLH YCSSLQHTQ EKIV
    CDS sequence
    • >TC004970 RA
      atggatata tttttgttc atgttccta gtttttata 
      tctttttta attggtatt atagtgact ctcgtggtg 
      caatattat attttgtat tcttatttt aaaacaagt 
      ccagtggtc acaccaaat gagaaaaag tgttctact 
      gattacgtt ttaccagag tctttgaaa aaacaattg 
      gatagtgaa gaattgaac gatgcgacg agtttatcg 
      gttagcctg gttttacag tttttgttt cacgaattg 
      aggcattct gaggctatt aaacgatgg ctttataag 
      aagctgagt cttgagttt gaggaacta ctaactaaa 
      actaccata gggaaattt ttcgaagtc attaatatt 
      agggatatg aacttaggt aacaacttt ccaaatatt 
      aaaaaaatc agtattgaa gatgtgaaa ttagataat 
      aaggaagga cacatagac actgtcagt ttatgtcta 
      gatgtagat tatacagga aacttttta cttagtgtt 
      gatgccaaa atgaaattt ggaaaaact gcatattta 
      tcaattaag gttaataaa gtcaacgga cttgtaaga 
      cttcagttt acaagacat ccgtacaca cactggtct 
      ttcagcttt tttcaagac cccattata gaattagaa 
      gttgaatca cactttcag ggacgtcaa ttacagtca 
      aacatcaca agtcttatc gtaaaccaa ataaaaaaa 
      gccatcaga cgaaaacac acactacca aattacaaa 
      atcagatac aaaccgttc tttatcaaa actgaccct 
      agccaacta gacacggac gatagcgaa atcgtccct 
      cagggccaa cttgaagtc acatgcata gatgtcaca 
      cgacttgag ttctcaaat gcggttcaa aacgtttat 
      tgcactgtt gcagttgac acgattcct tggatttgt 
      gtttatgaa tcagatgag cgcactttc atgattcta 
      gaaatcaca atgactaaa ttaaaacag gcccaactt 
      ggagtgatt ttcaagccg gaaaaaagc tcagtggct 
      gttgaaagc gtctctcca cacagttgc gccttcaaa 
      tctggcctc aaacccggc gatattgtt ttagcagtt 
      gagaataaa tcggtttcg agcgttccc caagtcgcc 
      aaatttatc aaatcagtg acttcgact catgtcact 
      cttcgagtg aaaagactg gtcgattcg tacgttttg 
      cgcaagaaa cacttcgat aaaaactca actgatgag 
      atggaccaa gacgaaagc agcttcttc attgttgat 
      agcgtggaa acgccgaaa aaaccggcg gagaaaatc 
      ccgaaaagt ctttcaagc aacgaaaac gtctcgaaa 
      tttgctcaa acgatcggt aattttagt ttgcgcaaa 
      cggaaggct tccgtttcg gataaatcg tcaacggaa 
      gtgagttgt aaaagtaca ccgacgtct agtagccct 
      ggaaccccg caacatgga gtgtttaaa caacataca 
      agcccgcta ttggctggg aaaaaacaa tcgatctcg 
      gaaataccc gaaattttg cgcgaaaat tccgaaagt 
      gaaattatc gaggtcggg aaaagcaaa gagttgggc 
      ccgacttcg gtgctttat ttcaatgaa gactttcaa 
      tttagcctc aaaaagggg ctcaagtac ttgaatgtc 
      aacgtttgg ggtacagtg actgatgat aaagatgtt 
      ttgttggga tatgcgaat attcccttg agccatatt 
      ttgaacgaa tgttttaat agcgttcta gggcactac 
      atgagacgg tactctttt ctaccgccg gcgttttgc 
      cattctaat aaccaatca cacccactc atggcacac 
      tctggcttc gaacacgtc ttttgctac ggagatatt 
      cttttgtct ttcatttgg tcgcatgag gacgatatc 
      gaagttaag cgtaaaatg tcgcaagat attttaaac 
      acagacatt gaacaaatt tcaacttca acagctctc 
      aagcacgac ttcatcaga acgcagttt caccgaaca 
      actcactgc gatttctgc tcgaaaaag atttggctc 
      aaagacgcg gtccagtgc cgcgaatgc ggtctctgt 
      tgccacaaa aaatgcata gccaagtgt caaacgagc 
      actgcatgc atccaaggt gacaaaaaa accgaaccg 
      gaagcggtc caaccggaa ataaccatg acggaacca 
      cccgacgaa atgaccgaa tatgcagaa ggggggttg 
      aaacgagta aacagtgtg aaaaattta gcaatacca 
      ggtgcgcat ttattgtca actgtttcg aaaagtttg 
      cctccatcg ccccaacga acgccaagt cgcaaacaa 
      tccatgtct aatataaat ccattcgct ttgtgtcct 
      ggtgtttta gaagacgtt cagaaaaaa ccggaagaa 
      gcctctgaa agtgtgaac cgacttttg gaacaagtc 
      atgcaatgc ccccctgat gagacgctc atggatgtg 
      gctaaagaa acgggaagg caactctat gccaatatg 
      ccacatgag gagaaagtt gaaaaaatc aacattatg 
      atggcagag ctgaagaag acgctagat tcagtgacc 
      atggagcat atggaactg tcgaaacag ctaagtagc 
      caagaatcg gacgctgag aagacgaaa ctggcgttt 
      ctgattggt caagccgat gctaaggtt caaggatta 
      tcagtattg atgcttcat tattgttcc agtttacag 
      catactcaa gaaaaaatt gtgtag
    mRNA sequence
    • >TC004970 RA
      tcttgagaa aaaaacaga aaaaggaaa atcatgttt 
      ggctttttt tgacgctta tcatatatg agataagca 
      caagggggc tataaaatg gtaaactga ctccacttt 
      gtgttattt cgaacaaaa cgggcctaa aaatggtga 
      taatataat ttaaagcgc tatggatat atttttgtt 
      catgttcct agtttttat atctttttt aattggtat 
      tatagtgac tctcgtggt gcaatatta tattttgta 
      ttcttattt taaaacaag tccagtggt cacaccaaa 
      tgagaaaaa gtgttctac tgattacgt tttaccaga 
      gtctttgaa aaaacaatt ggatagtga agaattgaa 
      cgatgcgac gagtttatc ggttagcct ggttttaca 
      gtttttgtt tcacgaatt gaggcattc tgaggctat 
      taaacgatg gctttataa gaagctgag tcttgagtt 
      tgaggaact actaactaa aactaccat agggaaatt 
      tttcgaagt cattaatat tagggatat gaacttagg 
      taacaactt tccaaatat taaaaaaat cagtattga 
      agatgtgaa attagataa taaggaagg acacataga 
      cactgtcag tttatgtct agatgtaga ttatacagg 
      aaacttttt acttagtgt tgatgccaa aatgaaatt 
      tggaaaaac tgcatattt atcaattaa ggttaataa 
      agtcaacgg acttgtaag acttcagtt tacaagaca 
      tccgtacac acactggtc tttcagctt ttttcaaga 
      ccccattat agaattaga agttgaatc acactttca 
      gggacgtca attacagtc aaacatcac aagtcttat 
      cgtaaacca aataaaaaa agccatcag acgaaaaca 
      cacactacc aaattacaa aatcagata caaaccgtt 
      ctttatcaa aactgaccc tagccaact agacacgga 
      cgatagcga aatcgtccc tcagggcca acttgaagt 
      cacatgcat agatgtcac acgacttga gttctcaaa 
      tgcggttca aaacgttta ttgcactgt tgcagttga 
      cacgattcc ttggatttg tgtttatga atcagatga 
      gcgcacttt catgattct agaaatcac aatgactaa 
      attaaaaca ggcccaact tggagtgat tttcaagcc 
      ggaaaaaag ctcagtggc tgttgaaag cgtctctcc 
      acacagttg cgccttcaa atctggcct caaacccgg 
      cgatattgt tttagcagt tgagaataa atcggtttc 
      gagcgttcc ccaagtcgc caaatttat caaatcagt 
      gacttcgac tcatgtcac tcttcgagt gaaaagact 
      ggtcgattc gtacgtttt gcgcaagaa acacttcga 
      taaaaactc aactgatga gatggacca agacgaaag 
      cagcttctt cattgttga tagcgtgga aacgccgaa 
      aaaaccggc ggagaaaat cccgaaaag tctttcaag 
      caacgaaaa cgtctcgaa atttgctca aacgatcgg 
      taattttag tttgcgcaa acggaaggc ttccgtttc 
      ggataaatc gtcaacgga agtgagttg taaaagtac 
      accgacgtc tagtagccc tggaacccc gcaacatgg 
      agtgtttaa acaacatac aagcccgct attggctgg 
      gaaaaaaca atcgatctc ggaaatacc cgaaatttt 
      gcgcgaaaa ttccgaaag tgaaattat cgaggtcgg 
      gaaaagcaa agagttggg cccgacttc ggtgcttta 
      tttcaatga agactttca atttagcct caaaaaggg 
      gctcaagta cttgaatgt caacgtttg gggtacagt 
      gactgatga taaagatgt tttgttggg atatgcgaa 
      tattccctt gagccatat tttgaacga atgttttaa 
      tagcgttct agggcacta catgagacg gtactcttt 
      tctaccgcc ggcgttttg ccattctaa taaccaatc 
      acacccact catggcaca ctctggctt cgaacacgt 
      cttttgcta cggagatat tcttttgtc tttcatttg 
      gtcgcatga ggacgatat cgaagttaa gcgtaaaat 
      gtcgcaaga tattttaaa cacagacat tgaacaaat 
      ttcaacttc aacagctct caagcacga cttcatcag 
      aacgcagtt tcaccgaac aactcactg cgatttctg 
      ctcgaaaaa gatttggct caaagacgc ggtccagtg 
      ccgcgaatg cggtctctg ttgccacaa aaaatgcat 
      agccaagtg tcaaacgag cactgcatg catccaagg 
      tgacaaaaa aaccgaacc ggaagcggt ccaaccgga 
      aataaccat gacggaacc acccgacga aatgaccga 
      atatgcaga aggggggtt gaaacgagt aaacagtgt 
      gaaaaattt agcaatacc aggtgcgca tttattgtc 
      aactgtttc gaaaagttt gcctccatc gccccaacg 
      aacgccaag tcgcaaaca atccatgtc taatataaa 
      tccattcgc tttgtgtcc tggtgtttt agaagacgt 
      tcagaaaaa accggaaga agcctctga aagtgtgaa 
      ccgactttt ggaacaagt catgcaatg cccccctga 
      tgagacgct catggatgt ggctaaaga aacgggaag 
      gcaactcta tgccaatat gccacatga ggagaaagt 
      tgaaaaaat caacattat gatggcaga gctgaagaa 
      gacgctaga ttcagtgac catggagca tatggaact 
      gtcgaaaca gctaagtag ccaagaatc ggacgctga 
      gaagacgaa actggcgtt tctgattgg tcaagccga 
      tgctaaggt tcaaggatt atcagtatt gatgcttca 
      ttattgttc cagtttaca gcatactca agaaaaaat 
      tgtgtaggc gctttttgt ctactaatt aaatttctt 
      gaaacaaaa aagcatatc atcgtgacc ccggtactt 
      aatttttgc atttatgac actcctcta gtcaattaa 
      ttttcacgt attgtagca tttttagtt tttacacgt 
      ttttaggaa cattaacta tacttactg tgatagcgt 
      aggatgtag taagataat ttttaatat ttatagtga 
      ctagatgta tgtgcaaaa ttgtctcta attattggt 
      tataatccc ggcaaaaag agtgaaata aagctttaa 
      cattgttgt attttttgc gtccatgtc agtatttta 
      gttttttat ttttttggc aacttggcc caacgttaa 
      tttgtgaac aaattcttt atgtaatat attttgtat 
      agtaaattt tgcctgctt cacataatg gtcttttat 
      ttcaaacaa ttacgaatg aaactatgg tttaatagg 
      taataaata aataaaggt aataaaggt acctccata 
      cctttatta aaaaattaa aaaagttta gacttgcgt 
      tttcttgga gtcatttct gaaatttat cttctgtga 
      tatagcaga aaatttaaa cattttttt gacggtact 
      tgctatttt aaaattata gaaaaatgg caacagcgc 
      acttacatg ttttggggt caaataggt cacaaataa 
      aaatttatg tgaaattag tgacaaatt tagtgttaa 
      gaaaactcc ctagttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY