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Gene information
General information
TC011638 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 15824453..15828212
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011638
    iBeetle screen iB_10643

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011638 RA
      MAGDGPHNK AIPASMVDP RSARQPPDN SPLQIFVKA 
      KKKINDIFG EIEDYVKDA VEYMHEIEK LQEIVNQER 
      VKKTEDFVS KVHGIRDVL SRDHMKVAF FGRTSNGKS 
      SVINAMLRD KILPSGIGH TTNCFLQVE GSPSDEPYL 
      VLEGSTEHC AVESVGQLA HALCKEKLN ESQMVRVYW 
      PRNRCLLLR DDVVFVDSP GVDVTPNLD EWIDKHCLD 
      ADVFVLVAN AESTLMVTE KNFFHKVST RLSKPNIFI 
      LNNRWDASA TEPEFLDQV RRQHQERAV DFLVKELKV 
      CTPKEAEER IFFISAKEV LQARLAEQG QTVSTPLTE 
      GFQTRYFEF QDFERKFEE CISKSAVKT KFEQHSQRG 
      KFIANELRE VLDDIYNDA QKLKDEKLR QKKEVHDKI 
      NYTEEQLII ITQEMKQKI HQMVEDVEQ RVSKALNEE 
      IRRLSTLVD EFNLPFHPE PLVLNVYKK ELHLHVETG 
      LGSNLKARL STALAMNME ASQREMTER MEKLLPANH 
      KAASNFILP RRQPFEILY RLNCDNLCA DFTEDLEFR 
      FSWGITTLI QRFAGSGAN FLALKNKRE SPQNSVIPS 
      TPTDQYDGR ILYGGQPSI IEDWSLISR LAIMGVSSQ 
      GTMGCLLVA GFLFKTVGW RIIAVTGAV YSCLYLYER 
      LTWTNKAKE KSFKKQYVN HATRKLKMI VDLTSANCS 
      HQVQQELSG TFARLCHVV DEATGEMDK EFKELEKDV 
      ATLENAAGR AKVLRNKAN YLAHELELF EDAYLKIHH
    CDS sequence
    • >TC011638 RA
      atggcgggc gatggcccc cacaataaa gccattcct 
      gcgagcatg gttgaccct agaagcgcc cgacagccc 
      cctgacaac tcccctttg cagattttt gtcaaagcc 
      aagaagaaa atcaacgat attttcggc gagattgaa 
      gactatgtt aaagacgca gtcgagtat atgcacgaa 
      atcgagaaa ctgcaagaa attgttaac caagaaagg 
      gttaagaaa actgaagac tttgtgagt aaagtgcac 
      ggaattcgc gatgtcttg agtcgtgac cacatgaaa 
      gttgcgttt tttggacgc acttccaac gggaaaagc 
      tcagttatt aatgcaatg ctgcgcgat aaaatcctt 
      cccagcgga attggtcac acgactaat tgtttccta 
      caggttgaa ggttcaccg tccgatgaa ccttacttg 
      gttcttgaa ggttccact gaacactgt gctgtggaa 
      tctgttggc caattagcg cacgctttg tgcaaagaa 
      aaattgaac gaatcgcaa atggtgcgc gtttattgg 
      ccgcgaaat cgctgtctt ttgttacga gacgatgtc 
      gtgttcgtt gattcgcca ggagttgac gttacgccg 
      aatttagac gaatggatc gacaagcat tgtttagat 
      gctgatgtt tttgtttta gtagcgaat gctgaatct 
      acacttatg gttacggaa aaaaatttc ttccataaa 
      gtctcgaca cgactctca aaaccaaac attttcatt 
      ttgaataat cgatgggac gcttctgcc accgaaccg 
      gaatttttg gatcaggtc agacgtcaa caccaagaa 
      cgtgccgtc gattttctt gtcaaagaa ctaaaagtt 
      tgtacaccg aaagaggcc gaagaacga attttcttt 
      atttcggcc aaagaagta ctacaagca cgattagct 
      gaacaagga caaaccgtt tcaacaccg ctaactgaa 
      ggtttccag acgcgttat ttcgaattt caggatttt 
      gaacgaaaa ttcgaagaa tgtatttcg aaatcggcc 
      gtcaaaacc aaattcgaa caacattca caacgcgga 
      aaattcata gcaaacgaa cttcgcgag gttttagac 
      gatatttac aacgacgcg caaaaacta aaagacgag 
      aaattgcgt caaaagaaa gaagtacac gacaaaatc 
      aactacact gaagagcaa ctaattata ataacacaa 
      gaaatgaag caaaaaatc caccaaatg gtcgaagac 
      gtcgaacag cgcgtttcc aaagcttta aacgaggaa 
      atccgtcgt ttgagtaca cttgttgac gaatttaat 
      ctccccttc catcctgaa cctttagtt ttgaacgtt 
      tacaaaaaa gaattgcat ttgcacgtc gaaaccggt 
      cttggttca aatttaaaa gcccgactt tcaacagct 
      ttagcaatg aacatggaa gcgagtcaa cgcgaaatg 
      accgaacgt atggaaaaa ttactacca gcaaatcac 
      aaagcggcg tctaatttt attttaccg cgtcggcaa 
      ccgtttgag attttgtat cggttgaat tgcgataat 
      ttatgcgct gattttacc gaagattta gaattccga 
      ttctcttgg gggattacc acgctaatt caacgattt 
      gccggcagt ggcgccaat ttcctcgcc ttgaaaaac 
      aaacgggaa agtccgcaa aattcagtc atcccctcc 
      actccaacc gaccaatac gatggtcgt attttatac 
      ggcggccag ccttcaatc attgaagat tggtcgttg 
      atttcgcga ttggctata atgggcgtg tcctcgcaa 
      ggtacaatg ggctgtctt ttagttgcc gggtttttg 
      tttaagaca gtcggttgg aggattatt gcagttacg 
      ggggctgtc tatagttgt ctctatctc tatgaacgt 
      ctcacttgg acgaataaa gccaaagaa aagagtttt 
      aagaaacaa tacgtcaat cacgctact agaaaattg 
      aaaatgatt gttgatttg acgtcggcg aattgtagt 
      catcaagtg cagcaagag ttgagcgga acttttgct 
      cgattgtgt cacgtggtc gatgaagca acgggcgaa 
      atggataag gaattcaaa gagttggag aaagatgtg 
      gcaacgttg gaaaatgca gcaggaagg gctaaagtt 
      ttaaggaac aaagccaat tatttggca catgagttg 
      gagttgttc gaggatgcg tatcttaag atacaccat 
      taa
    mRNA sequence
    • >TC011638 RA
      caacgtcaa aaattaaaa actcgtgct ttggaggaa 
      tcgcctcac aaaaataat aattacgta atcaaataa 
      cacaaaatt tgctgctgc tagacgcat ggtagattg 
      gcaccccct tgtttgatg gccgcttat ataaaccgc 
      acaatttcg ttgatggcg ggcgatggc ccccacaat 
      aaagccatt cctgcgagc atggttgac cctagaagc 
      gcccgacag ccccctgac aactcccct ttgcagatt 
      tttgtcaaa gccaagaag aaaatcaac gatattttc 
      ggcgagatt gaagactat gttaaagac gcagtcgag 
      tatatgcac gaaatcgag aaactgcaa gaaattgtt 
      aaccaagaa agggttaag aaaactgaa gactttgtg 
      agtaaagtg cacggaatt cgcgatgtc ttgagtcgt 
      gaccacatg aaagttgcg ttttttgga cgcacttcc 
      aacgggaaa agctcagtt attaatgca atgctgcgc 
      gataaaatc cttcccagc ggaattggt cacacgact 
      aattgtttc ctacaggtt gaaggttca ccgtccgat 
      gaaccttac ttggttctt gaaggttcc actgaacac 
      tgtgctgtg gaatctgtt ggccaatta gcgcacgct 
      ttgtgcaaa gaaaaattg aacgaatcg caaatggtg 
      cgcgtttat tggccgcga aatcgctgt cttttgtta 
      cgagacgat gtcgtgttc gttgattcg ccaggagtt 
      gacgttacg ccgaattta gacgaatgg atcgacaag 
      cattgttta gatgctgat gtttttgtt ttagtagcg 
      aatgctgaa tctacactt atggttacg gaaaaaaat 
      ttcttccat aaagtctcg acacgactc tcaaaacca 
      aacattttc attttgaat aatcgatgg gacgcttct 
      gccaccgaa ccggaattt ttggatcag gtcagacgt 
      caacaccaa gaacgtgcc gtcgatttt cttgtcaaa 
      gaactaaaa gtttgtaca ccgaaagag gccgaagaa 
      cgaattttc tttatttcg gccaaagaa gtactacaa 
      gcacgatta gctgaacaa ggacaaacc gtttcaaca 
      ccgctaact gaaggtttc cagacgcgt tatttcgaa 
      tttcaggat tttgaacga aaattcgaa gaatgtatt 
      tcgaaatcg gccgtcaaa accaaattc gaacaacat 
      tcacaacgc ggaaaattc atagcaaac gaacttcgc 
      gaggtttta gacgatatt tacaacgac gcgcaaaaa 
      ctaaaagac gagaaattg cgtcaaaag aaagaagta 
      cacgacaaa atcaactac actgaagag caactaatt 
      ataataaca caagaaatg aagcaaaaa atccaccaa 
      atggtcgaa gacgtcgaa cagcgcgtt tccaaagct 
      ttaaacgag gaaatccgt cgtttgagt acacttgtt 
      gacgaattt aatctcccc ttccatcct gaaccttta 
      gttttgaac gtttacaaa aaagaattg catttgcac 
      gtcgaaacc ggtcttggt tcaaattta aaagcccga 
      ctttcaaca gctttagca atgaacatg gaagcgagt 
      caacgcgaa atgaccgaa cgtatggaa aaattacta 
      ccagcaaat cacaaagcg gcgtctaat tttatttta 
      ccgcgtcgg caaccgttt gagattttg tatcggttg 
      aattgcgat aatttatgc gctgatttt accgaagat 
      ttagaattc cgattctct tgggggatt accacgcta 
      attcaacga tttgccggc agtggcgcc aatttcctc 
      gccttgaaa aacaaacgg gaaagtccg caaaattca 
      gtcatcccc tccactcca accgaccaa tacgatggt 
      cgtatttta tacggcggc cagccttca atcattgaa 
      gattggtcg ttgatttcg cgattggct ataatgggc 
      gtgtcctcg caaggtaca atgggctgt cttttagtt 
      gccgggttt ttgtttaag acagtcggt tggaggatt 
      attgcagtt acgggggct gtctatagt tgtctctat 
      ctctatgaa cgtctcact tggacgaat aaagccaaa 
      gaaaagagt tttaagaaa caatacgtc aatcacgct 
      actagaaaa ttgaaaatg attgttgat ttgacgtcg 
      gcgaattgt agtcatcaa gtgcagcaa gagttgagc 
      ggaactttt gctcgattg tgtcacgtg gtcgatgaa 
      gcaacgggc gaaatggat aaggaattc aaagagttg 
      gagaaagat gtggcaacg ttggaaaat gcagcagga 
      agggctaaa gttttaagg aacaaagcc aattatttg 
      gcacatgag ttggagttg ttcgaggat gcgtatctt 
      aagatacac cattaagtt gtgatagtt tatgtatga 
      tattggagc aattggcgt tttcaaatc tctgctcgt 
      tattttgcc agagggaca ttaaaatag tggtgatta 
      tttaattta aggttgaac tgtgcaaac gaaaagaat 
      ttcaaattg gatttttat gaaaaataa ttttaaagt 
      ccaaaccac attagcaac agtacatat ttatttgaa 
      aagtttttt tcgtgtaaa aataaaatg agaaaacat 
      ga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY