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Gene information
General information
TC008794 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9962808..9971956
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008794
    iBeetle screen iB_10642

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    labrum slightly size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008794 RA
      MVWQPSSVL VNGLILLTF SLHSFVTAT NPSSAKFYT 
      IHWNSSNPI FRIDNTDNV IDVNRYNLK FEYDQVNLI 
      CPVYVPGTR DEEMEKYII YNVSKDEYD TCRITNPNP 
      RIIAVCDKP YKLMYFTIT FRPFTPQPG GLEFLPGHD 
      YYFISTSTS DDLHRRIGG RCTTHNMKI VFKVWGPAQ 
      TSPPPAPAT VSRWWPVTS PRSTSTSST TTTTSTPPP 
      VTTTKKSKT YGGKHPNEV GKSEELTLG GGAAVGPVQ 
      VLVVCAVAV LLAMR
    CDS sequence
    • >TC008794 RA
      atggtctgg caaccgagc agtgtcctt gtcaacggc 
      ctgatcctg ctaacgttc agcctacac agtttcgtc 
      acggcaacg aaccccagc agcgccaag ttctacacc 
      atccactgg aactcctcg aacccgatc tttcgcatc 
      gacaacacc gacaacgtg atcgacgtg aaccgctac 
      aacctgaag ttcgagtac gaccaggtg aacctcatc 
      tgccccgtg tacgtcccg ggcacccgc gacgaggag 
      atggagaag tacatcatc tacaacgtg tccaaggac 
      gagtacgac acgtgccgc ataacgaac cccaacccg 
      cgcatcatt gccgtctgt gacaaaccg tataagtta 
      atgtacttc accataacg ttccggccg ttcacgccg 
      cagccgggg ggcctggag ttcctcccc gggcacgac 
      tactacttc atctcgacg tcgaccagc gacgacctg 
      caccggcga ataggcggc cggtgcacg acgcacaac 
      atgaagatc gtgttcaag gtgtggggg cccgcgcag 
      acgtcgccg ccgcccgcg cccgccacc gtctcgcgg 
      tggtggccc gtcacgtcg ccgcgctcc acctccacg 
      agctcgacg acgacgacg accagcacg ccgccgccg 
      gtcacgacg acgaagaag tccaagacc tacggcggc 
      aagcacccc aacgaggtc ggcaagagc gaggaactc 
      acgctcggc ggcggcgcc gccgtcggg ccggtgcaa 
      gtgctggtg gtgtgtgcg gtcgcggtg ctgctcgcg 
      atgcgatga
    mRNA sequence
    • >TC008794 RA
      gcgcgtttg tgtaggtcg ccgtcaggt ggcggcgcg 
      atcgtcgag agaagctga gtgacaaaa tggcaatgg 
      aatgttggt gtaagaacc cgactttat gatgtgttt 
      tcgttaaat ttctaagat aattgtggt gttgttgaa 
      tgtaatatg tttagtaaa cataattta acataatgt 
      gcattgtgg ggctcgatg tgtgagtgc atttcggcc 
      gggtttatc atgtttctt ttccaatta tgaaccata 
      tacttttaa caatagatt gacgctgga ggagacatg 
      taaatcgtt ttttcaccc gtctctgaa gaagaggaa 
      ggcatcacg atggtctgg caaccgagc agtgtcctt 
      gtcaacggc ctgatcctg ctaacgttc agcctacac 
      agtttcgtc acggcaacg aaccccagc agcgccaag 
      ttctacacc atccactgg aactcctcg aacccgatc 
      tttcgcatc gacaacacc gacaacgtg atcgacgtg 
      aaccgctac aacctgaag ttcgagtac gaccaggtg 
      aacctcatc tgccccgtg tacgtcccg ggcacccgc 
      gacgaggag atggagaag tacatcatc tacaacgtg 
      tccaaggac gagtacgac acgtgccgc ataacgaac 
      cccaacccg cgcatcatt gccgtctgt gacaaaccg 
      tataagtta atgtacttc accataacg ttccggccg 
      ttcacgccg cagccgggg ggcctggag ttcctcccc 
      gggcacgac tactacttc atctcgacg tcgaccagc 
      gacgacctg caccggcga ataggcggc cggtgcacg 
      acgcacaac atgaagatc gtgttcaag gtgtggggg 
      cccgcgcag acgtcgccg ccgcccgcg cccgccacc 
      gtctcgcgg tggtggccc gtcacgtcg ccgcgctcc 
      acctccacg agctcgacg acgacgacg accagcacg 
      ccgccgccg gtcacgacg acgaagaag tccaagacc 
      tacggcggc aagcacccc aacgaggtc ggcaagagc 
      gaggaactc acgctcggc ggcggcgcc gccgtcggg 
      ccggtgcaa gtgctggtg gtgtgtgcg gtcgcggtg 
      ctgctcgcg atgcgatga aatttttcg cggtactaa 
      gtagttaag atgattaaa taataaaga acgttacgt 
      gtccctttg tgtgtaatg tactgtcac gagttattt 
      ttaatgagg aaaaacgac ctaaaacta tcctaattt 
      tatataaat attggtaac tattaggcg attaaggta 
      aattattgt tcccacttc tcattttcg atcgtcggt 
      tttagatgc aaaccagtc ctcggattt attattgtg 
      tatatttta gtaacgaaa ccgacgact gaaacctga 
      caatataat tttttattc gattatcca ttattttta 
      atatgaatt ggtggtgta cagtttttt atatgttgg 
      tgtagacac tgaagataa taaaataag aaaaataat 
      ttaatgcgc aagcgcatt gtgaccaat tcaacggac 
      taaacaaaa aatgtgtaa atgttatat ttttcggtc 
      agttgatta tttcgtaga ttataaaca gtgttgaac 
      atttcaaat aacggcgta tttgtataa ctgtgaaga 
      ctgataata gtttttatt gtgttaaat ataaagtat 
      taatatcct taatagcat aatcttggc gtttttagt 
      cgacatttc actcaacct ccatgtact tattacgcg 
      gtgacagtt ttgttttac agtaaatgg ttgtgatta 
      tttaccctt ttatagtgc cagtttaca aattagact 
      aatgcagac ttccacttt atttattaa tttatttat 
      tttctctct atttataca tcacggtag ctttgtaga 
      tagtaaatt atttcacct taaaaacta aacagggtg 
      tcgaattaa cacgctgtt tagtagtta cttttattc 
      ttcaggata gcccggtga aatttacac tttgcatag 
      gatttgttc gtgtcaatt atcctgaag tgaggtaga 
      tttgtgcaa tataagctt tcaataata ctcatcaat 
      tctctctat cacactgtc cattttgat cttgccatg 
      taaaattaa ggctttaat cgaacaata ctccatcgc 
      actaaaaac gccagattg aagatgatt tgtgtctag 
      aaaagtagt tttacgata ttgtaatca gatgtactt 
      ttaattgca agccatagt gataaatat ttaacttat 
      aaaaaacga aaatagcaa tgataccaa ctgatcttg 
      gtttattta agtttttaa catatttat tgggtgacg 
      tttagtaac ttctgcaaa cacataaac atgtaaacg 
      gtcgcttct tgacgactc catatattt ttccttaaa 
      actatttta tcgaaatgc tctgtatat atttgtaat 
      tggttttaa catacgaaa tttgtgtta cattttaat 
      aggtaaaca cactgtatg tattacgat tgtaaaatg 
      cactcacta tatatacat aatattata aatatacat 
      acatgccat tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY