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Gene information
General information
TC009021 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8616136..8617854
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC009021
      iBeetle screen iB_10640

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection

      Fertility
      mature egg not present - Number of animals showing the phenotype: 3

      previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 2

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 20 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC009021 RA
        MSKLFTVCL SCLLLLFLQ LSTNYAQSP STDSSTDPP 
ITTTTDSTP STTEETVPP TTTETPSSL PTTTTPQTE 
TPTITEAPT TETPTTTEA PTTETPAPT PIPPHRITE 
SFTWSSRKL DQNPIVVTV PDGYFISVT VSGVNMANG 
NYLLIQGGE SYSDQATDG KVFANTVEG PVSYLFKTS 
AVYGYCETA NKNAADEEP LYDFVAVFE VAGDGPTTT 
VTTTSTTPI TVPTTEKGA STYVTVNIS GKALNDYYE 
ETTVDAFKE CVVTMATNY CKDQNIYLT HDITNAYVM 
ISEISRCPY SWPHSETCV RITFKLPIF YNESSSVQD 
YQLNTERLE DMWLRWDQL DDQCFSNHS FSVYEEPDI 
EQSIVWWAI GISIVLLVF IALLWFLKS FSAKVKSVI 
KKRMRRHSD TKSIISQRE SYSSLTPHY LQDHLDTPK 
LFEG
      CDS sequence
      • >TC009021 RA
        atgtcaaag ttatttacc gtttgtttg tcctgttta 
ttattacta tttcttcag ttatcaaca aattatgca 
caaagccca tctactgat tcttctaca gacccgcct 
ataactact actacagac tccacccca tctaccact 
gaagaaact gttccacct accaccact gaaactcct 
tcttcccta cctaccaca acaactcct caaactgaa 
acaccgaca ataactgaa gccccgact actgaaact 
cctaccact actgaagct ccgactact gaaacgccc 
gctccaacc ccgatccct ccccacagg atcaccgaa 
tccttcacg tggagctcc agaaaactg gaccaaaat 
ccaattgtt gtaacagtt cccgatgga tatttcatt 
tcggtcacc gtttctggt gttaatatg gccaatgga 
aactactta ctaattcaa ggaggagaa tcatactca 
gaccaagca accgatggt aaagttttt gcaaacaca 
gtggaaggt cctgtgagt tatttgttc aaaacttct 
gctgtttat ggctattgt gaaacggca aataaaaac 
gcagccgat gaggagcct ttgtatgac tttgtggca 
gtatttgaa gtcgcgggt gatggacct acaacaact 
gtgaccacc acttcaacg actccaatt accgttcct 
acgacagaa aaaggggct tccacttac gtcactgta 
aacatctct ggcaaagct ttaaatgat tactacgaa 
gaaaccact gtagacgca ttcaaagag tgtgttgtt 
acaatggcc acaaattat tgcaaagac caaaatatt 
tacctcact catgacatt accaatgct tatgtgatg 
atctccgaa atatctcgt tgtccttac tcgtggcca 
cacagcgaa acctgtgtc cgaatcacc tttaaatta 
ccaattttc tataacgaa tcttccagc gttcaagat 
tatcagtta aataccgaa cgtctggaa gacatgtgg 
ctacgttgg gaccaatta gacgatcag tgtttcagc 
aatcattca ttttcagtt tatgaggaa ccggatatt 
gaacaatca atcgtttgg tgggcaatc ggtataagc 
attgttttg cttgttttc attgctttg ttgtggttt 
ttgaagagt ttcagtgcg aaagttaaa tctgtgatt 
aaaaagaga atgaggcga cacagcgat acaaaatcg 
attatttcg caacgggag agttacagt tcattgact 
ccacactat ctgcaagat catctagac acaccaaag 
ctttttgaa ggataa
      mRNA sequence
      • >TC009021 RA
        tcaggaaag attatttcc gttgagact atatcttat 
catttaact ctaatcaga ttttttcag tgttactaa 
ttaaagtta ttttttgta catttttgt gtaattctt 
attgaacaa aatgtcaaa gttatttac cgtttgttt 
gtcctgttt attattact atttcttca gttatcaac 
aaattatgc acaaagccc atctactga ttcttctac 
agacccgcc tataactac tactacaga ctccacccc 
atctaccac tgaagaaac tgttccacc taccaccac 
tgaaactcc ttcttccct acctaccac aacaactcc 
tcaaactga aacaccgac aataactga agccccgac 
tactgaaac tcctaccac tactgaagc tccgactac 
tgaaacgcc cgctccaac cccgatccc tccccacag 
gatcaccga atccttcac gtggagctc cagaaaact 
ggaccaaaa tccaattgt tgtaacagt tcccgatgg 
atatttcat ttcggtcac cgtttctgg tgttaatat 
ggccaatgg aaactactt actaattca aggaggaga 
atcatactc agaccaagc aaccgatgg taaagtttt 
tgcaaacac agtggaagg tcctgtgag ttatttgtt 
caaaacttc tgctgttta tggctattg tgaaacggc 
aaataaaaa cgcagccga tgaggagcc tttgtatga 
ctttgtggc agtatttga agtcgcggg tgatggacc 
tacaacaac tgtgaccac cacttcaac gactccaat 
taccgttcc tacgacaga aaaaggggc ttccactta 
cgtcactgt aaacatctc tggcaaagc tttaaatga 
ttactacga agaaaccac tgtagacgc attcaaaga 
gtgtgttgt tacaatggc cacaaatta ttgcaaaga 
ccaaaatat ttacctcac tcatgacat taccaatgc 
ttatgtgat gatctccga aatatctcg ttgtcctta 
ctcgtggcc acacagcga aacctgtgt ccgaatcac 
ctttaaatt accaatttt ctataacga atcttccag 
cgttcaaga ttatcagtt aaataccga acgtctgga 
agacatgtg gctacgttg ggaccaatt agacgatca 
gtgtttcag caatcattc attttcagt ttatgagga 
accggatat tgaacaatc aatcgtttg gtgggcaat 
cggtataag cattgtttt gcttgtttt cattgcttt 
gttgtggtt tttgaagag tttcagtgc gaaagttaa 
atctgtgat taaaaagag aatgaggcg acacagcga 
tacaaaatc gattatttc gcaacggga gagttacag 
ttcattgac tccacacta tctgcaaga tcatctaga 
cacaccaaa gctttttga aggataaca ctgcatttt 
tgagcggtt ttgtttttt actcaaaat ttaatgtat 
taaaaaaat acagtcact aaataatg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY