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Gene information
General information
TC001865 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 520803..529610
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001865
    iBeetle screen iB_10639

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001865 RA
      MMTSVTLPS TSGPLHGGY SSVAEETTS SEPEFETPR 
      PVKRCLVTP LTDSCKKRR KQSTPVRIS AAESDVHTE 
      GKPSSTSPE PELQCPVCQ LFFTSREQL QSHVVSEHS 
      ALECKKEPE EFALNPPER AENAHQEVP WMNELQGKE 
      WINPGLHGL PFNPTNPMF MTLPQFAQP ENMPRQPIR 
      IFNPDAYCD LCNKEFCNK YFLKTHKAN KHGIYSDPP 
      SSEPLVNNV NIASYAGTI KLPTSVPSV EISAVKSEK 
      PANIISPFN NLFPNPFSN KSFPQKIKP NLPNLYESE 
      SNENVPNSN GFSSNCESD KVSPDDQPT EESSSPRNI 
      QTESTSFQK DSEGGVYYI SQSKMSPGN DIEISNRLK 
      RIGVMNPKA FCEICCKEY CNKYFLRTH KMKRHGIYI 
      PDDKDKDTK VEGSGNFPW QNNMQTSPL NLIMTEQSN 
      QDRKTTSPS DISCDICGI KFQNASLSQ LHNVSVHSK 
      IYPKEEEHQ EQQTEEAKK ETTQEKPIN AEAISEDLQ 
      KLQTMILQL NELDVSKVC TTCNACNKE FENRYFLHA 
      HMMTEHGIL LEDVTDADK PVEPENSSN NNTMCDLCG 
      KDVQSVEEM KKHLLEFHP NQQTSTDNA KDEFNGFST 
      PEKSVNRIP VSSAERRIS MNVTPTSSY CDICNKELC 
      NKYFMKTHM QRMHGIEIE NGAQIGGVI CDICNKELC 
      SKYFLRVHK HNTHGIVEY GTSLLQPRK SEGESAHLP 
      PEPDPALKP ADLADLSHR YFTHFTEVC SICSRRFRS 
      TKWLKAHLL SDHGQAGAE KWSELEQQL QQTIGQHSK 
      GVKSERGSP TLKIPNGGQ ERKELEIQN VLSSLFGVD 
      ESSTKTYSC SYCPFTTPL LPLLFVHER SHSMSSDGA 
      PLKCPVCAQ SFLQPELLQ HHMFAQHPF LPLPPFFNG 
      NENPGETSK EPSDNADDD DAKTKKKGK GKRAELSPE 
      VGQSLKDVA KRAQVPAAY ALPQGGEDA GYVMQAFLL 
      EEAAPERRL VPAVVFLPV FQKQPAPLT VTFTLTPA
    CDS sequence
    • >TC001865 RA
      atgatgaca tccgtcacg ttaccctca acttctggt 
      cccttacac ggcggatac tcctcagta gccgaggaa 
      acaacaagc agcgagccc gagttcgaa acacctcgt 
      ccagtgaag cgttgtctc gtaacacca ctgaccgat 
      tcatgcaag aagcggcgc aaacaatcg acaccagtc 
      cgaatttca gcagccgaa tccgacgtt cataccgaa 
      ggtaagccc agttcgacc tcccccgag cccgagctg 
      cagtgccct gtgtgccaa ttattcttc acgagtcgt 
      gagcagctt caaagtcat gtcgtatcc gagcattct 
      gcgctcgaa tgcaagaaa gagccggaa gaattcgcg 
      ctgaatccg ccagaacgc gccgaaaac gcgcaccag 
      gaggtgcca tggatgaac gagctgcag ggcaaagag 
      tggatcaac cccggtttg cacggcttg cccttcaac 
      ccaacgaat ccgatgttc atgacctta cctcaattc 
      gcgcaaccc gagaacatg cccagacaa ccgatccga 
      attttcaac ccggatgcg tattgtgac ttgtgcaac 
      aaggagttc tgtaacaaa tattttttg aagactcac 
      aaggccaac aagcacggc atctattcc gatcctccc 
      tcatcagag cctctagtg aataatgta aatattgct 
      tcgtatgct ggaacgatt aaactccca acttccgtg 
      ccaagtgta gaaatatca gccgttaaa agcgaaaaa 
      cccgccaat attatcagt cccttcaac aacttgttc 
      cccaatccc ttctcaaac aagtccttc ccgcagaaa 
      atcaagccg aacctcccc aacttatac gaaagtgaa 
      tcaaacgaa aatgttcct aattcaaat ggtttctca 
      tcaaactgt gaaagtgat aaagtatcg ccagatgac 
      caaccaact gaagaatct tctagtcct agaaatata 
      caaaccgaa tctacctca tttcaaaaa gattctgaa 
      ggaggagtc tactacatt tcccaaagc aaaatgtct 
      ccaggcaac gatatcgag atatccaat cgcctcaaa 
      agaatcggt gttatgaat cctaaagca ttttgtgag 
      atttgttgc aaggaatat tgcaataaa tatttccta 
      cgaacccat aaaatgaaa cgtcacggt atctacata 
      cctgacgat aaagataaa gacactaaa gtcgaagga 
      agtggcaat tttccatgg caaaacaac atgcaaaca 
      agtcctctg aacttaata atgacagaa caatcaaac 
      caagatcgt aaaactacc tcgccatct gacatcagt 
      tgtgacata tgtggaatc aaattccaa aacgcgagt 
      ctatcgcaa ctgcacaac gtcagtgtc cattcgaaa 
      atttacccc aaagaggag gaacaccaa gagcagcaa 
      accgaagaa gcgaagaaa gaaacgacc caagaaaag 
      ccaataaac gcggaagcc attagcgaa gacctgcaa 
      aaactgcaa acaatgatc ttacaattg aacgagctc 
      gacgtcagt aaagtatgc acgacttgc aacgcctgt 
      aacaaagag tttgagaat cgttatttt ttacacgcc 
      cacatgatg acagaacac ggaatatta ctcgaagac 
      gttacagac gctgataaa cctgttgag ccagaaaac 
      agttctaat aacaatacc atgtgcgat ttgtgtggg 
      aaagacgtt cagagtgtc gaagaaatg aaaaagcac 
      ttgcttgaa ttccatcct aatcagcaa accagcacc 
      gataacgcc aaagacgag tttaacgga tttagcact 
      ccggaaaaa agcgttaac aggatacct gtatcaagt 
      gccgagcgg cgaattagc atgaacgtg acaccgacc 
      agtagttat tgcgacatt tgcaataaa gaattgtgt 
      aataaatat ttcatgaag acgcacatg caaagaatg 
      cacggcatc gagatcgaa aacggcgcg caaattggc 
      ggtgttatt tgcgatata tgcaataag gagttgtgc 
      agtaaatat ttcctcaga gttcacaag cacaatact 
      cacgggatt gtcgaatac ggaacgagt ctcctccag 
      ccgaggaag agcgaaggc gagagcgct cacctgccg 
      cctgaaccg gatccggcg ctgaagcct gcagatttg 
      gccgatttg agccaccgc tacttcact catttcacg 
      gaagtttgc tcgatttgc agccgccgt tttcgcagc 
      acgaagtgg ctgaaggcc cacttgctg agcgaccac 
      ggccaagcc ggcgccgaa aagtggtcg gagttggag 
      cagcagctc cagcagacc atcggccag cattcgaaa 
      ggcgtcaag agcgagcgt ggcagtccc actctcaaa 
      atcccaaac ggcggccaa gagcgcaaa gaactcgag 
      atccagaac gtcctatcg agtctgttc ggggttgac 
      gaatccagt acaaaaact tattcatgt tcctattgc 
      ccttttacg actcctctc cttccgcta ctgttcgtg 
      catgagagg tcccactcg atgtcatcg gacggcgcc 
      cctctgaag tgccccgtg tgcgcccaa agcttcctg 
      cagccagaa ctgttgcag catcacatg tttgctcaa 
      catccgttt cttcccttg ccgccgttc tttaacggg 
      aacgagaat cctggcgag acgtccaag gagccgtcc 
      gacaacgcc gacgacgac gacgccaag accaagaag 
      aaggggaag gggaagagg gccgagctg tcgccggaa 
      gtcggccag agtctgaaa gacgtcgcg aaacgggcg 
      caagttcct gctgcatat gcgctgcct caagggggc 
      gaggatgcg gggtatgtg atgcaagcg tttttgcta 
      gaggaggct gcccccgag aggaggctc gtgccggcg 
      gtggtgttc ctgccggtg ttccagaag cagcccgca 
      cctctcacc gttacattc actctcacc ccggcctaa
    mRNA sequence
    • >TC001865 RA
      tcgcaatga ctattttct aaaattcgg ctgtggtct 
      ttggaccta tagaatcat tgcatgtcg cctacacga 
      caatatgat gacatccgt cacgttacc ctcaacttc 
      tggtccctt acacggcgg atactcctc agtagccga 
      ggaaacaac aagcagcga gcccgagtt cgaaacacc 
      tcgtccagt gaagcgttg tctcgtaac accactgac 
      cgattcatg caagaagcg gcgcaaaca atcgacacc 
      agtccgaat ttcagcagc cgaatccga cgttcatac 
      cgaaggtaa gcccagttc gacctcccc cgagcccga 
      gctgcagtg ccctgtgtg ccaattatt cttcacgag 
      tcgtgagca gcttcaaag tcatgtcgt atccgagca 
      ttctgcgct cgaatgcaa gaaagagcc ggaagaatt 
      cgcgctgaa tccgccaga acgcgccga aaacgcgca 
      ccaggaggt gccatggat gaacgagct gcagggcaa 
      agagtggat caaccccgg tttgcacgg cttgccctt 
      caacccaac gaatccgat gttcatgac cttacctca 
      attcgcgca acccgagaa catgcccag acaaccgat 
      ccgaatttt caacccgga tgcgtattg tgacttgtg 
      caacaagga gttctgtaa caaatattt tttgaagac 
      tcacaaggc caacaagca cggcatcta ttccgatcc 
      tccctcatc agagcctct agtgaataa tgtaaatat 
      tgcttcgta tgctggaac gattaaact cccaacttc 
      cgtgccaag tgtagaaat atcagccgt taaaagcga 
      aaaacccgc caatattat cagtccctt caacaactt 
      gttccccaa tcccttctc aaacaagtc cttcccgca 
      gaaaatcaa gccgaacct ccccaactt atacgaaag 
      tgaatcaaa cgaaaatgt tcctaattc aaatggttt 
      ctcatcaaa ctgtgaaag tgataaagt atcgccaga 
      tgaccaacc aactgaaga atcttctag tcctagaaa 
      tatacaaac cgaatctac ctcatttca aaaagattc 
      tgaaggagg agtctacta catttccca aagcaaaat 
      gtctccagg caacgatat cgagatatc caatcgcct 
      caaaagaat cggtgttat gaatcctaa agcattttg 
      tgagatttg ttgcaagga atattgcaa taaatattt 
      cctacgaac ccataaaat gaaacgtca cggtatcta 
      catacctga cgataaaga taaagacac taaagtcga 
      aggaagtgg caattttcc atggcaaaa caacatgca 
      aacaagtcc tctgaactt aataatgac agaacaatc 
      aaaccaaga tcgtaaaac tacctcgcc atctgacat 
      cagttgtga catatgtgg aatcaaatt ccaaaacgc 
      gagtctatc gcaactgca caacgtcag tgtccattc 
      gaaaattta ccccaaaga ggaggaaca ccaagagca 
      gcaaaccga agaagcgaa gaaagaaac gacccaaga 
      aaagccaat aaacgcgga agccattag cgaagacct 
      gcaaaaact gcaaacaat gatcttaca attgaacga 
      gctcgacgt cagtaaagt atgcacgac ttgcaacgc 
      ctgtaacaa agagtttga gaatcgtta ttttttaca 
      cgcccacat gatgacaga acacggaat attactcga 
      agacgttac agacgctga taaacctgt tgagccaga 
      aaacagttc taataacaa taccatgtg cgatttgtg 
      tgggaaaga cgttcagag tgtcgaaga aatgaaaaa 
      gcacttgct tgaattcca tcctaatca gcaaaccag 
      caccgataa cgccaaaga cgagtttaa cggatttag 
      cactccgga aaaaagcgt taacaggat acctgtatc 
      aagtgccga gcggcgaat tagcatgaa cgtgacacc 
      gaccagtag ttattgcga catttgcaa taaagaatt 
      gtgtaataa atatttcat gaagacgca catgcaaag 
      aatgcacgg catcgagat cgaaaacgg cgcgcaaat 
      tggcggtgt tatttgcga tatatgcaa taaggagtt 
      gtgcagtaa atatttcct cagagttca caagcacaa 
      tactcacgg gattgtcga atacggaac gagtctcct 
      ccagccgag gaagagcga aggcgagag cgctcacct 
      gccgcctga accggatcc ggcgctgaa gcctgcaga 
      tttggccga tttgagcca ccgctactt cactcattt 
      cacggaagt ttgctcgat ttgcagccg ccgttttcg 
      cagcacgaa gtggctgaa ggcccactt gctgagcga 
      ccacggcca agccggcgc cgaaaagtg gtcggagtt 
      ggagcagca gctccagca gaccatcgg ccagcattc 
      gaaaggcgt caagagcga gcgtggcag tcccactct 
      caaaatccc aaacggcgg ccaagagcg caaagaact 
      cgagatcca gaacgtcct atcgagtct gttcggggt 
      tgacgaatc cagtacaaa aacttattc atgttccta 
      ttgcccttt tacgactcc tctccttcc gctactgtt 
      cgtgcatga gaggtccca ctcgatgtc atcggacgg 
      cgcccctct gaagtgccc cgtgtgcgc ccaaagctt 
      cctgcagcc agaactgtt gcagcatca catgtttgc 
      tcaacatcc gtttcttcc cttgccgcc gttctttaa 
      cgggaacga gaatcctgg cgagacgtc caaggagcc 
      gtccgacaa cgccgacga cgacgacgc caagaccaa 
      gaagaaggg gaaggggaa gagggccga gctgtcgcc 
      ggaagtcgg ccagagtct gaaagacgt cgcgaaacg 
      ggcgcaagt tcctgctgc atatgcgct gcctcaagg 
      gggcgagga tgcggggta tgtgatgca agcgttttt 
      gctagagga ggctgcccc cgagaggag gctcgtgcc 
      ggcggtggt gttcctgcc ggtgttcca gaagcagcc 
      cgcacctct caccgttac attcactct caccccggc 
      ctaagcagt ttcctacac gtagtttgc atagatgtg 
      acatatttt tttaagaaa ctatcgatg gcgattcga 
      cgacacctg tgatttttg attaatttt atttgatta 
      tattgtata atcttaaag tatatataa gcgtattcc 
      tatatttgc aagaggcct catatatta tggggaaca 
      tatgagcac atattttta tgcaaatac cggaaagac 
      tgagtgaaa ttttatata gtctgttga ttttcactt 
      taaaatatc gagatactg atgttttgt acgatagaa 
      agctttagg tgatgttta aagaagttc ctattttat 
      ctctcgata tacttattc gttcacaag tgtgtatgt 
      attgagttg ttagatgta gcttgctca acagtcagt 
      taacgttcc gcattccca gtccacgca actcaggtt 
      catggtaaa attcatcga aaccgcccc cgccaggcg 
      gaccccaaa cccaacacc cacacctcc accgacgtc 
      ttacgtcct actccaacc aaaatttat acaattgca 
      aacacaact aacgctgtt ttcatttgg agttattta 
      atattcgtg ggcttattg caaaacgtg accaaaaat 
      taagccttt tcacaaatc ctatttaga aaatcagtg 
      tttttttct acaaaaaca cttttggtg ataaattac 
      ataagacca agagctaag ttttgtaca ccaaatctc 
      ctcaagatt tcctctttg atatttaat aataatatc 
      ctcctgatt ttctgaaga ttttgcaat atttttcct 
      aattttttt aaatgattt tctctgcgt atgttttga 
      agttttccg aaaaagtct gatttggtc tctttttgc 
      actaagctt atgatgtac tagattcag tgaaattaa 
      aatggaaaa aataggaat gaattgcaa gttgggtaa 
      ttctggtgg tggtgaaaa tcgggtgtg gggttgggc 
      cgcgtccgc gggggtccc cggacaaga gcaactcag 
      ggttagctg aaacgagta caatcagac ctcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY