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Gene information
General information
TC003529 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 6263502..6265307
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003529
    iBeetle screen iB_10638

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature potentially thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07282

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003529 RA
      MKYFFLISL LVFPAVIFT LDNGLKMVA HAEEDPLED 
      EVEVEGEAD VEEVAPTDA EDEEDSEKT TASPDADTT 
      LLFVRPIAT ASQLELPAG VPVEFLVGF RNKGKEDFV 
      LESLEASFR YPMDFNFYI QNFSAIGYS KVVQPEQEA 
      TLAYSFIPS EAFAGRPFG LNINLAYRD ASGNSFQEA 
      VYNETVQII ELEEGLDGE TFFLYVFLV AGVVLLLVI 
      GQQTLLSFG KKRITRKAA PVETGTSNP NNVDYDWLP 
      KQTLATLNK EKSQKSPKS IKQSPRQRK VKRSTGSE
    CDS sequence
    • >TC003529 RA
      atgaagtac tttttccta atttccttg ctggttttc 
      cctgctgtg attttcacc cttgataat ggccttaaa 
      atggtcgca catgccgag gaagacccc ctagaggac 
      gaggttgaa gttgaggga gaagcagat gttgaagag 
      gtggcaccg actgatgcg gaggacgaa gaggattcg 
      gagaagaca acagcgtcg ccagatgct gatacgacg 
      cttctgttt gttcgcccg atcgccacc gcttcacaa 
      ttagaactg cctgccggg gttcctgtt gaatttttg 
      gtgggtttc cgcaacaag ggcaaagag gattttgtc 
      ctggaatct ctggaagcg tcgttccgt tatcccatg 
      gatttcaac ttctacatt cagaatttc tcagcgatt 
      ggctacagc aaagtggtg cagccggag caagaagcc 
      acgctggcc tacagtttc atcccgtct gaggccttc 
      gctggtagg ccgtttggc ctgaatatt aatctcgct 
      tacagggat gcttctggg aattcgttc caagaagcc 
      gtttacaac gagactgta caaataatt gagcttgaa 
      gaaggtttg gatggagaa actttcttc ttgtacgtg 
      tttcttgtc gccggtgtt gtacttctt ttggttatt 
      gggcagcag actttgttg tcttttggt aaaaaacga 
      atcactcgc aaagctgcg cctgttgag actggtaca 
      agtaaccca aataatgtg gactatgac tggttgcca 
      aaacaaacg ttggccact ttgaacaaa gaaaaatca 
      caaaagtct cccaaatca attaagcag agtcccaga 
      caacgcaaa gtcaaaagg tccactggt tctgaatag
    mRNA sequence
    • >TC003529 RA
      attgcaagt tatggtcac ttaaaagat taaatttag 
      acaacctct cggcgattt atcgcttca cggaggagc 
      cgcaacaac acgtgaaaa acaagcgtt ttaacctca 
      aaaaacgtt gacaaacgt ttggtgaaa acgcagggt 
      ttttggttt cgtggtgtt ggggtcccg cttgttgac 
      gttttgaga caagtcgtg acgtcacga gagaggggg 
      tgccatgtg tacaagcaa cttccacgt cttactcgc 
      tggctggaa aagttgaac ttgtagtga gagtttctt 
      tggttagct tcacatcgc cgaatgata ttagtgaga 
      ttagttcag taaaacaac acaaaaatg aagtacttt 
      ttcctaatt tccttgctg gttttccct gctgtgatt 
      ttcaccctt gataatggc cttaaaatg gtcgcacat 
      gccgaggaa gacccccta gaggacgag gttgaagtt 
      gagggagaa gcagatgtt gaagaggtg gcaccgact 
      gatgcggag gacgaagag gattcggag aagacaaca 
      gcgtcgcca gatgctgat acgacgctt ctgtttgtt 
      cgcccgatc gccaccgct tcacaatta gaactgcct 
      gccggggtt cctgttgaa tttttggtg ggtttccgc 
      aacaagggc aaagaggat tttgtcctg gaatctctg 
      gaagcgtcg ttccgttat cccatggat ttcaacttc 
      tacattcag aatttctca gcgattggc tacagcaaa 
      gtggtgcag ccggagcaa gaagccacg ctggcctac 
      agtttcatc ccgtctgag gccttcgct ggtaggccg 
      tttggcctg aatattaat ctcgcttac agggatgct 
      tctgggaat tcgttccaa gaagccgtt tacaacgag 
      actgtacaa ataattgag cttgaagaa ggtttggat 
      ggagaaact ttcttcttg tacgtgttt cttgtcgcc 
      ggtgttgta cttcttttg gttattggg cagcagact 
      ttgttgtct tttggtaaa aaacgaatc actcgcaaa 
      gctgcgcct gttgagact ggtacaagt aacccaaat 
      aatgtggac tatgactgg ttgccaaaa caaacgttg 
      gccactttg aacaaagaa aaatcacaa aagtctccc 
      aaatcaatt aagcagagt cccagacaa cgcaaagtc 
      aaaaggtcc actggttct gaatagtcg ttttcaaaa 
      gtttttgcg ttcatttca cagtttgtt ttcacattt 
      cattttgta cattgacag ggacacaaa acttgtaag 
      aataatata aatagactc ctatttata attattttg 
      tatcctgtg ttgtgattt ttttttaat tgttgaatt 
      tttcacata ggtatagac ttaaattat gtaaataat 
      gaatgatcg ttttgaaaa acgatttga ataaataaa 
      cggaaacat c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY