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Gene information
General information
TC015419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 612804..623521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015419
    iBeetle screen iB_10637

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature mostly degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna potentially slightlysize decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015419 RA
      MIFTLYYAY ATIMQWWRL RTLRNKLNG TLNDQNALL 
      VCSGHSVHD SRKQRSLEN LLYSTENRI ACTVVPKQK 
      LNLSQWIAF PKNLRYFPI FIDDNKISV SRPQIFLLL 
      QAVLDNYVK HQSEIIQVE HFGELIGWK ASDSFEVVV 
      KTDMDHLTK LVNQFSQSD VDINHELKL LKIETVDVE 
      NTPTVIKYE KKYLKEQTL KYSYAPVHW GKFVQEVKD 
      LYSKIRDIT TPKIKIENG SPQCLESAK PIIKKEPKK 
      TDPLSEDKN TLDVSRRHK KPREGSSSK PSKPSKHKT 
      KSDEKQHKH KRTANKLVK QSSVKENEE VKSEVKEKV 
      ITGGTPKPP NVLVYADSL VTKDNVKRV LFSILNKEK 
      YTIYDLPTS KNTGWDKST VLVVVCGGV PPDLTANLI 
      QYFLTGGQL LCICSDLMY SVLYTFTTA EVREHELVR 
      FSYGQWQHV KMMHHIFCY QASPAKKQF SKDSDHSSN 
      GSSPQAPRT PSNVELRHN EKDYNIQVQ VLGTEETWH 
      TPSLLLAKV KNSQGRAVF SQVHLEIDP DVYENDEDK 
      YRELLSSNE ARLEILKDI ISNHLDLVC DESLGTVEY 
      SQGYFLGRY DAKMNMMTD CDSITNNQL TCDKISVVF 
      CGHNVEPGL ASNIRLPVM IHSCPSNFS TLDYFETLN 
      TEDIGRLVI YADILTSTQ HLLDKKLRH GLVVIARQQ 
      THGMGRSSN SWLSPLGCA AFSLQMHIP LDSHMGQSL 
      PLIQHLVMV AVISSIKNV QGLDCLNVG IKWPNDLYA 
      EGAIKIGGL VVTSTIFSN FAIVNIGCG VNLSNQNPT 
      ICINDLIEK NTVMQPIAY EKYFAAVFN EIERLYFDV 
      QKNGFEAFF QLYYKYWLH SDADVTIQS EKGDSTRAK 
      IVGIDKYGF LSVRLADGS MTSVQPDGN SFDLMSGLI 
      APKKF
    CDS sequence
    • >TC015419 RA
      atgatcttt actttatat tatgcctac gccaccata 
      atgcagtgg tggcgtctc cgaacttta cgcaacaaa 
      ctcaacgga acactaaac gatcaaaat gccttgctt 
      gtctgttct gggcattcg gtgcacgat agcagaaaa 
      cagcgaagt ttggagaat ttattgtac agtactgag 
      aataggatc gcgtgcaca gttgtgcct aagcaaaaa 
      ctaaactta agtcaatgg atcgcattt ccgaaaaat 
      ctaagatac ttcccgatt tttatcgac gacaataaa 
      atcagtgtt agtcggccc caaattttt ctcctcctt 
      caggccgtt ttagacaat tatgttaaa catcagagt 
      gagattatt caagttgag cattttggc gaactgatc 
      ggttggaaa gcgagcgat tcgttcgaa gttgtggtt 
      aaaacagac atggatcat ttgactaag ctggtcaat 
      cagtttagc caaagcgat gttgatatt aaccatgaa 
      ttaaagtta cttaaaatt gaaactgtt gatgttgaa 
      aatacaccc actgtcatc aaatacgag aaaaaatat 
      ctcaaagaa caaactttg aaatattca tatgctcca 
      gttcattgg gggaaattc gtgcaagaa gtcaaggac 
      ttgtactcg aaaatacgc gacattacc actccgaaa 
      ataaaaatc gaaaatggt tcgcctcag tgcctcgaa 
      tccgcaaaa cccataata aaaaaagaa ccaaaaaaa 
      accgatcca ctttccgaa gacaaaaac acacttgat 
      gtgtcacga cgtcacaaa aaaccacgt gaaggcagt 
      tcttccaaa ccatcaaaa ccatcaaag cacaaaaca 
      aaaagcgac gaaaaacaa cacaaacac aaacgaact 
      gcaaataaa ttagtcaaa cagagctca gttaaagaa 
      aacgaagag gtcaaaagt gaagtcaaa gaaaaggtt 
      ataacaggg ggaaccccc aaaccacca aatgtcctc 
      gtttacgcc gacagtttg gtcacgaaa gacaacgtc 
      aaaagagtc ctattctca attttgaat aaggaaaag 
      tacacaatc tacgacctc cccacttcg aaaaacacg 
      ggttgggac aaatccaca gttctggtg gttgtgtgt 
      ggtggtgtc ccacctgac ctcaccgcc aacctgatc 
      caatatttc ctcaccgga ggtcaactc ctctgcatt 
      tgcagcgac ctcatgtac agtgtactt tacacgttc 
      acgacggcg gaagtacgc gagcacgaa ctcgtccgc 
      ttctcgtac ggccaatgg cagcacgtt aaaatgatg 
      catcatatt ttctgctat caagcatcg cctgctaag 
      aaacagttc agtaaagat tcggatcat tcgagtaac 
      ggctcaagt ccgcaagcg ccaaggacg ccctctaac 
      gtcgaatta agacacaac gaaaaagat tataacatt 
      caagttcaa gtgttaggg actgaagaa acgtggcat 
      acgcccagc ctgctattg gctaaagtg aaaaactcg 
      caagggagg gctgttttt tcgcaagtg catctggaa 
      attgatcca gatgtttat gagaatgat gaggataag 
      taccgggag ttgttgagt agtaatgaa gccagattg 
      gagattttg aaagatatt atttccaat catttggat 
      ttggtgtgt gatgagagt ttgggcacg gttgagtac 
      agtcaaggg tattttctt gggcgttat gatgcaaaa 
      atgaatatg atgaccgac tgtgactcc ataaccaat 
      aaccaatta acttgtgat aaaatcagt gttgttttt 
      tgtggccat aatgtcgag cctggcctt gcgtcaaat 
      atccgcctt ccggtcatg attcattcg tgtccttcg 
      aacttctcc actttggat tatttcgaa acgttgaac 
      actgaagac attggccgt ttggtgatt tatgccgat 
      attttgaca agtacccag cacctactt gacaaaaaa 
      ttgcgccat ggtcttgtt gtgatcgcc cggcaacag 
      acccatggc atgggacgg tcgagcaac tcatggttg 
      agtccattg ggttgtgcg gccttttcc cttcagatg 
      cacattccg ctggatagt cacatggga cagtcgctt 
      cccttgatc cagcactta gtcatggta gctgtcata 
      tcgtcgatt aaaaatgtc caaggtttg gattgtctc 
      aatgttggt attaaatgg cctaatgat ttgtacgcg 
      gaaggggcg attaaaatc ggtggactt gttgtaact 
      tctacgata ttttcaaat tttgctatt gttaatata 
      ggttgtggt gtaaattta tcaaaccaa aaccccaca 
      atatgtata aatgatctc atagaaaaa aacacagtg 
      atgcaacct atagcttat gagaaatat ttcgcagct 
      gtgttcaat gaaatcgaa cgcttatac tttgatgtg 
      cagaaaaat gggtttgag gcgtttttc cagctttat 
      tacaagtat tggttgcac agcgatgcc gacgttacg 
      atacagtcg gaaaagggc gattccaca cgagctaaa 
      atcgtggga atcgacaaa tacggtttt ctctctgtc 
      cgattggcc gacggcagt atgacgtca gtccagccc 
      gacggtaat tcgttcgat ttgatgagc ggcttgata 
      gcgccaaag aaattttaa
    mRNA sequence
    • >TC015419 RA
      gaattttta attattgtg gtcttgaga atttttttt 
      aatgatagg tgcaatgga ggcataaaa cataatgat 
      ctttacttt atattatgc ctacgccac cataatgca 
      gtggtggcg tctccgaac tttacgcaa caaactcaa 
      cggaacact aaacgatca aaatgcctt gcttgtctg 
      ttctgggca ttcggtgca cgatagcag aaaacagcg 
      aagtttgga gaatttatt gtacagtac tgagaatag 
      gatcgcgtg cacagttgt gcctaagca aaaactaaa 
      cttaagtca atggatcgc atttccgaa aaatctaag 
      atacttccc gatttttat cgacgacaa taaaatcag 
      tgttagtcg gccccaaat ttttctcct ccttcaggc 
      cgttttaga caattatgt taaacatca gagtgagat 
      tattcaagt tgagcattt tggcgaact gatcggttg 
      gaaagcgag cgattcgtt cgaagttgt ggttaaaac 
      agacatgga tcatttgac taagctggt caatcagtt 
      tagccaaag cgatgttga tattaacca tgaattaaa 
      gttacttaa aattgaaac tgttgatgt tgaaaatac 
      acccactgt catcaaata cgagaaaaa atatctcaa 
      agaacaaac tttgaaata ttcatatgc tccagttca 
      ttgggggaa attcgtgca agaagtcaa ggacttgta 
      ctcgaaaat acgcgacat taccactcc gaaaataaa 
      aatcgaaaa tggttcgcc tcagtgcct cgaatccgc 
      aaaacccat aataaaaaa agaaccaaa aaaaaccga 
      tccactttc cgaagacaa aaacacact tgatgtgtc 
      acgacgtca caaaaaacc acgtgaagg cagttcttc 
      caaaccatc aaaaccatc aaagcacaa aacaaaaag 
      cgacgaaaa acaacacaa acacaaacg aactgcaaa 
      taaattagt caaacagag ctcagttaa agaaaacga 
      agaggtcaa aagtgaagt caaagaaaa ggttataac 
      agggggaac ccccaaacc accaaatgt cctcgttta 
      cgccgacag tttggtcac gaaagacaa cgtcaaaag 
      agtcctatt ctcaatttt gaataagga aaagtacac 
      aatctacga cctccccac ttcgaaaaa cacgggttg 
      ggacaaatc cacagttct ggtggttgt gtgtggtgg 
      tgtcccacc tgacctcac cgccaacct gatccaata 
      tttcctcac cggaggtca actcctctg catttgcag 
      cgacctcat gtacagtgt actttacac gttcacgac 
      ggcggaagt acgcgagca cgaactcgt ccgcttctc 
      gtacggcca atggcagca cgttaaaat gatgcatca 
      tattttctg ctatcaagc atcgcctgc taagaaaca 
      gttcagtaa agattcgga tcattcgag taacggctc 
      aagtccgca agcgccaag gacgccctc taacgtcga 
      attaagaca caacgaaaa agattataa cattcaagt 
      tcaagtgtt agggactga agaaacgtg gcatacgcc 
      cagcctgct attggctaa agtgaaaaa ctcgcaagg 
      gagggctgt tttttcgca agtgcatct ggaaattga 
      tccagatgt ttatgagaa tgatgagga taagtaccg 
      ggagttgtt gagtagtaa tgaagccag attggagat 
      tttgaaaga tattatttc caatcattt ggatttggt 
      gtgtgatga gagtttggg cacggttga gtacagtca 
      agggtattt tcttgggcg ttatgatgc aaaaatgaa 
      tatgatgac cgactgtga ctccataac caataacca 
      attaacttg tgataaaat cagtgttgt tttttgtgg 
      ccataatgt cgagcctgg ccttgcgtc aaatatccg 
      ccttccggt catgattca ttcgtgtcc ttcgaactt 
      ctccacttt ggattattt cgaaacgtt gaacactga 
      agacattgg ccgtttggt gatttatgc cgatatttt 
      gacaagtac ccagcacct acttgacaa aaaattgcg 
      ccatggtct tgttgtgat cgcccggca acagaccca 
      tggcatggg acggtcgag caactcatg gttgagtcc 
      attgggttg tgcggcctt ttcccttca gatgcacat 
      tccgctgga tagtcacat gggacagtc gcttccctt 
      gatccagca cttagtcat ggtagctgt catatcgtc 
      gattaaaaa tgtccaagg tttggattg tctcaatgt 
      tggtattaa atggcctaa tgatttgta cgcggaagg 
      ggcgattaa aatcggtgg acttgttgt aacttctac 
      gatattttc aaattttgc tattgttaa tataggttg 
      tggtgtaaa tttatcaaa ccaaaaccc cacaatatg 
      tataaatga tctcataga aaaaaacac agtgatgca 
      acctatagc ttatgagaa atatttcgc agctgtgtt 
      caatgaaat cgaacgctt atactttga tgtgcagaa 
      aaatgggtt tgaggcgtt tttccagct ttattacaa 
      gtattggtt gcacagcga tgccgacgt tacgataca 
      gtcggaaaa gggcgattc cacacgagc taaaatcgt 
      gggaatcga caaatacgg ttttctctc tgtccgatt 
      ggccgacgg cagtatgac gtcagtcca gcccgacgg 
      taattcgtt cgatttgat gagcggctt gatagcgcc 
      aaagaaatt ttaatcctt cggtgtttt ttctatttt 
      tttagtatg atgtaatta ttggtcttt ttgatcttt 
      gttgttgtc tcttggtgt acacgcaaa ttgtaagag 
      gaaaaaaat ataaaatgt tgacgaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY