General information | |||
TC015419 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 612804..623521 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015419 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_10637 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larval musculature mostly degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
antenna potentially slightlysize decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015419 RA
MIFTLYYAY ATIMQWWRL RTLRNKLNG TLNDQNALL VCSGHSVHD SRKQRSLEN LLYSTENRI ACTVVPKQK LNLSQWIAF PKNLRYFPI FIDDNKISV SRPQIFLLL QAVLDNYVK HQSEIIQVE HFGELIGWK ASDSFEVVV KTDMDHLTK LVNQFSQSD VDINHELKL LKIETVDVE NTPTVIKYE KKYLKEQTL KYSYAPVHW GKFVQEVKD LYSKIRDIT TPKIKIENG SPQCLESAK PIIKKEPKK TDPLSEDKN TLDVSRRHK KPREGSSSK PSKPSKHKT KSDEKQHKH KRTANKLVK QSSVKENEE VKSEVKEKV ITGGTPKPP NVLVYADSL VTKDNVKRV LFSILNKEK YTIYDLPTS KNTGWDKST VLVVVCGGV PPDLTANLI QYFLTGGQL LCICSDLMY SVLYTFTTA EVREHELVR FSYGQWQHV KMMHHIFCY QASPAKKQF SKDSDHSSN GSSPQAPRT PSNVELRHN EKDYNIQVQ VLGTEETWH TPSLLLAKV KNSQGRAVF SQVHLEIDP DVYENDEDK YRELLSSNE ARLEILKDI ISNHLDLVC DESLGTVEY SQGYFLGRY DAKMNMMTD CDSITNNQL TCDKISVVF CGHNVEPGL ASNIRLPVM IHSCPSNFS TLDYFETLN TEDIGRLVI YADILTSTQ HLLDKKLRH GLVVIARQQ THGMGRSSN SWLSPLGCA AFSLQMHIP LDSHMGQSL PLIQHLVMV AVISSIKNV QGLDCLNVG IKWPNDLYA EGAIKIGGL VVTSTIFSN FAIVNIGCG VNLSNQNPT ICINDLIEK NTVMQPIAY EKYFAAVFN EIERLYFDV QKNGFEAFF QLYYKYWLH SDADVTIQS EKGDSTRAK IVGIDKYGF LSVRLADGS MTSVQPDGN SFDLMSGLI APKKF
- >TC015419 RA
atgatcttt actttatat tatgcctac gccaccata atgcagtgg tggcgtctc cgaacttta cgcaacaaa ctcaacgga acactaaac gatcaaaat gccttgctt gtctgttct gggcattcg gtgcacgat agcagaaaa cagcgaagt ttggagaat ttattgtac agtactgag aataggatc gcgtgcaca gttgtgcct aagcaaaaa ctaaactta agtcaatgg atcgcattt ccgaaaaat ctaagatac ttcccgatt tttatcgac gacaataaa atcagtgtt agtcggccc caaattttt ctcctcctt caggccgtt ttagacaat tatgttaaa catcagagt gagattatt caagttgag cattttggc gaactgatc ggttggaaa gcgagcgat tcgttcgaa gttgtggtt aaaacagac atggatcat ttgactaag ctggtcaat cagtttagc caaagcgat gttgatatt aaccatgaa ttaaagtta cttaaaatt gaaactgtt gatgttgaa aatacaccc actgtcatc aaatacgag aaaaaatat ctcaaagaa caaactttg aaatattca tatgctcca gttcattgg gggaaattc gtgcaagaa gtcaaggac ttgtactcg aaaatacgc gacattacc actccgaaa ataaaaatc gaaaatggt tcgcctcag tgcctcgaa tccgcaaaa cccataata aaaaaagaa ccaaaaaaa accgatcca ctttccgaa gacaaaaac acacttgat gtgtcacga cgtcacaaa aaaccacgt gaaggcagt tcttccaaa ccatcaaaa ccatcaaag cacaaaaca aaaagcgac gaaaaacaa cacaaacac aaacgaact gcaaataaa ttagtcaaa cagagctca gttaaagaa aacgaagag gtcaaaagt gaagtcaaa gaaaaggtt ataacaggg ggaaccccc aaaccacca aatgtcctc gtttacgcc gacagtttg gtcacgaaa gacaacgtc aaaagagtc ctattctca attttgaat aaggaaaag tacacaatc tacgacctc cccacttcg aaaaacacg ggttgggac aaatccaca gttctggtg gttgtgtgt ggtggtgtc ccacctgac ctcaccgcc aacctgatc caatatttc ctcaccgga ggtcaactc ctctgcatt tgcagcgac ctcatgtac agtgtactt tacacgttc acgacggcg gaagtacgc gagcacgaa ctcgtccgc ttctcgtac ggccaatgg cagcacgtt aaaatgatg catcatatt ttctgctat caagcatcg cctgctaag aaacagttc agtaaagat tcggatcat tcgagtaac ggctcaagt ccgcaagcg ccaaggacg ccctctaac gtcgaatta agacacaac gaaaaagat tataacatt caagttcaa gtgttaggg actgaagaa acgtggcat acgcccagc ctgctattg gctaaagtg aaaaactcg caagggagg gctgttttt tcgcaagtg catctggaa attgatcca gatgtttat gagaatgat gaggataag taccgggag ttgttgagt agtaatgaa gccagattg gagattttg aaagatatt atttccaat catttggat ttggtgtgt gatgagagt ttgggcacg gttgagtac agtcaaggg tattttctt gggcgttat gatgcaaaa atgaatatg atgaccgac tgtgactcc ataaccaat aaccaatta acttgtgat aaaatcagt gttgttttt tgtggccat aatgtcgag cctggcctt gcgtcaaat atccgcctt ccggtcatg attcattcg tgtccttcg aacttctcc actttggat tatttcgaa acgttgaac actgaagac attggccgt ttggtgatt tatgccgat attttgaca agtacccag cacctactt gacaaaaaa ttgcgccat ggtcttgtt gtgatcgcc cggcaacag acccatggc atgggacgg tcgagcaac tcatggttg agtccattg ggttgtgcg gccttttcc cttcagatg cacattccg ctggatagt cacatggga cagtcgctt cccttgatc cagcactta gtcatggta gctgtcata tcgtcgatt aaaaatgtc caaggtttg gattgtctc aatgttggt attaaatgg cctaatgat ttgtacgcg gaaggggcg attaaaatc ggtggactt gttgtaact tctacgata ttttcaaat tttgctatt gttaatata ggttgtggt gtaaattta tcaaaccaa aaccccaca atatgtata aatgatctc atagaaaaa aacacagtg atgcaacct atagcttat gagaaatat ttcgcagct gtgttcaat gaaatcgaa cgcttatac tttgatgtg cagaaaaat gggtttgag gcgtttttc cagctttat tacaagtat tggttgcac agcgatgcc gacgttacg atacagtcg gaaaagggc gattccaca cgagctaaa atcgtggga atcgacaaa tacggtttt ctctctgtc cgattggcc gacggcagt atgacgtca gtccagccc gacggtaat tcgttcgat ttgatgagc ggcttgata gcgccaaag aaattttaa
- >TC015419 RA
gaattttta attattgtg gtcttgaga atttttttt aatgatagg tgcaatgga ggcataaaa cataatgat ctttacttt atattatgc ctacgccac cataatgca gtggtggcg tctccgaac tttacgcaa caaactcaa cggaacact aaacgatca aaatgcctt gcttgtctg ttctgggca ttcggtgca cgatagcag aaaacagcg aagtttgga gaatttatt gtacagtac tgagaatag gatcgcgtg cacagttgt gcctaagca aaaactaaa cttaagtca atggatcgc atttccgaa aaatctaag atacttccc gatttttat cgacgacaa taaaatcag tgttagtcg gccccaaat ttttctcct ccttcaggc cgttttaga caattatgt taaacatca gagtgagat tattcaagt tgagcattt tggcgaact gatcggttg gaaagcgag cgattcgtt cgaagttgt ggttaaaac agacatgga tcatttgac taagctggt caatcagtt tagccaaag cgatgttga tattaacca tgaattaaa gttacttaa aattgaaac tgttgatgt tgaaaatac acccactgt catcaaata cgagaaaaa atatctcaa agaacaaac tttgaaata ttcatatgc tccagttca ttgggggaa attcgtgca agaagtcaa ggacttgta ctcgaaaat acgcgacat taccactcc gaaaataaa aatcgaaaa tggttcgcc tcagtgcct cgaatccgc aaaacccat aataaaaaa agaaccaaa aaaaaccga tccactttc cgaagacaa aaacacact tgatgtgtc acgacgtca caaaaaacc acgtgaagg cagttcttc caaaccatc aaaaccatc aaagcacaa aacaaaaag cgacgaaaa acaacacaa acacaaacg aactgcaaa taaattagt caaacagag ctcagttaa agaaaacga agaggtcaa aagtgaagt caaagaaaa ggttataac agggggaac ccccaaacc accaaatgt cctcgttta cgccgacag tttggtcac gaaagacaa cgtcaaaag agtcctatt ctcaatttt gaataagga aaagtacac aatctacga cctccccac ttcgaaaaa cacgggttg ggacaaatc cacagttct ggtggttgt gtgtggtgg tgtcccacc tgacctcac cgccaacct gatccaata tttcctcac cggaggtca actcctctg catttgcag cgacctcat gtacagtgt actttacac gttcacgac ggcggaagt acgcgagca cgaactcgt ccgcttctc gtacggcca atggcagca cgttaaaat gatgcatca tattttctg ctatcaagc atcgcctgc taagaaaca gttcagtaa agattcgga tcattcgag taacggctc aagtccgca agcgccaag gacgccctc taacgtcga attaagaca caacgaaaa agattataa cattcaagt tcaagtgtt agggactga agaaacgtg gcatacgcc cagcctgct attggctaa agtgaaaaa ctcgcaagg gagggctgt tttttcgca agtgcatct ggaaattga tccagatgt ttatgagaa tgatgagga taagtaccg ggagttgtt gagtagtaa tgaagccag attggagat tttgaaaga tattatttc caatcattt ggatttggt gtgtgatga gagtttggg cacggttga gtacagtca agggtattt tcttgggcg ttatgatgc aaaaatgaa tatgatgac cgactgtga ctccataac caataacca attaacttg tgataaaat cagtgttgt tttttgtgg ccataatgt cgagcctgg ccttgcgtc aaatatccg ccttccggt catgattca ttcgtgtcc ttcgaactt ctccacttt ggattattt cgaaacgtt gaacactga agacattgg ccgtttggt gatttatgc cgatatttt gacaagtac ccagcacct acttgacaa aaaattgcg ccatggtct tgttgtgat cgcccggca acagaccca tggcatggg acggtcgag caactcatg gttgagtcc attgggttg tgcggcctt ttcccttca gatgcacat tccgctgga tagtcacat gggacagtc gcttccctt gatccagca cttagtcat ggtagctgt catatcgtc gattaaaaa tgtccaagg tttggattg tctcaatgt tggtattaa atggcctaa tgatttgta cgcggaagg ggcgattaa aatcggtgg acttgttgt aacttctac gatattttc aaattttgc tattgttaa tataggttg tggtgtaaa tttatcaaa ccaaaaccc cacaatatg tataaatga tctcataga aaaaaacac agtgatgca acctatagc ttatgagaa atatttcgc agctgtgtt caatgaaat cgaacgctt atactttga tgtgcagaa aaatgggtt tgaggcgtt tttccagct ttattacaa gtattggtt gcacagcga tgccgacgt tacgataca gtcggaaaa gggcgattc cacacgagc taaaatcgt gggaatcga caaatacgg ttttctctc tgtccgatt ggccgacgg cagtatgac gtcagtcca gcccgacgg taattcgtt cgatttgat gagcggctt gatagcgcc aaagaaatt ttaatcctt cggtgtttt ttctatttt tttagtatg atgtaatta ttggtcttt ttgatcttt gttgttgtc tcttggtgt acacgcaaa ttgtaagag gaaaaaaat ataaaatgt tgacgaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
