Status
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Gene information
General information
TC015419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 612804..623521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015419
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10637

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature mostly degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna potentially slightlysize decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015419 RA
      MIFTLYYAY ATIMQWWRL RTLRNKLNG TLNDQNALL 
VCSGHSVHD SRKQRSLEN LLYSTENRI ACTVVPKQK 
LNLSQWIAF PKNLRYFPI FIDDNKISV SRPQIFLLL 
QAVLDNYVK HQSEIIQVE HFGELIGWK ASDSFEVVV 
KTDMDHLTK LVNQFSQSD VDINHELKL LKIETVDVE 
NTPTVIKYE KKYLKEQTL KYSYAPVHW GKFVQEVKD 
LYSKIRDIT TPKIKIENG SPQCLESAK PIIKKEPKK 
TDPLSEDKN TLDVSRRHK KPREGSSSK PSKPSKHKT 
KSDEKQHKH KRTANKLVK QSSVKENEE VKSEVKEKV 
ITGGTPKPP NVLVYADSL VTKDNVKRV LFSILNKEK 
YTIYDLPTS KNTGWDKST VLVVVCGGV PPDLTANLI 
QYFLTGGQL LCICSDLMY SVLYTFTTA EVREHELVR 
FSYGQWQHV KMMHHIFCY QASPAKKQF SKDSDHSSN 
GSSPQAPRT PSNVELRHN EKDYNIQVQ VLGTEETWH 
TPSLLLAKV KNSQGRAVF SQVHLEIDP DVYENDEDK 
YRELLSSNE ARLEILKDI ISNHLDLVC DESLGTVEY 
SQGYFLGRY DAKMNMMTD CDSITNNQL TCDKISVVF 
CGHNVEPGL ASNIRLPVM IHSCPSNFS TLDYFETLN 
TEDIGRLVI YADILTSTQ HLLDKKLRH GLVVIARQQ 
THGMGRSSN SWLSPLGCA AFSLQMHIP LDSHMGQSL 
PLIQHLVMV AVISSIKNV QGLDCLNVG IKWPNDLYA 
EGAIKIGGL VVTSTIFSN FAIVNIGCG VNLSNQNPT 
ICINDLIEK NTVMQPIAY EKYFAAVFN EIERLYFDV 
QKNGFEAFF QLYYKYWLH SDADVTIQS EKGDSTRAK 
IVGIDKYGF LSVRLADGS MTSVQPDGN SFDLMSGLI 
APKKF
    CDS sequence
    • >TC015419 RA
      atgatcttt actttatat tatgcctac gccaccata 
atgcagtgg tggcgtctc cgaacttta cgcaacaaa 
ctcaacgga acactaaac gatcaaaat gccttgctt 
gtctgttct gggcattcg gtgcacgat agcagaaaa 
cagcgaagt ttggagaat ttattgtac agtactgag 
aataggatc gcgtgcaca gttgtgcct aagcaaaaa 
ctaaactta agtcaatgg atcgcattt ccgaaaaat 
ctaagatac ttcccgatt tttatcgac gacaataaa 
atcagtgtt agtcggccc caaattttt ctcctcctt 
caggccgtt ttagacaat tatgttaaa catcagagt 
gagattatt caagttgag cattttggc gaactgatc 
ggttggaaa gcgagcgat tcgttcgaa gttgtggtt 
aaaacagac atggatcat ttgactaag ctggtcaat 
cagtttagc caaagcgat gttgatatt aaccatgaa 
ttaaagtta cttaaaatt gaaactgtt gatgttgaa 
aatacaccc actgtcatc aaatacgag aaaaaatat 
ctcaaagaa caaactttg aaatattca tatgctcca 
gttcattgg gggaaattc gtgcaagaa gtcaaggac 
ttgtactcg aaaatacgc gacattacc actccgaaa 
ataaaaatc gaaaatggt tcgcctcag tgcctcgaa 
tccgcaaaa cccataata aaaaaagaa ccaaaaaaa 
accgatcca ctttccgaa gacaaaaac acacttgat 
gtgtcacga cgtcacaaa aaaccacgt gaaggcagt 
tcttccaaa ccatcaaaa ccatcaaag cacaaaaca 
aaaagcgac gaaaaacaa cacaaacac aaacgaact 
gcaaataaa ttagtcaaa cagagctca gttaaagaa 
aacgaagag gtcaaaagt gaagtcaaa gaaaaggtt 
ataacaggg ggaaccccc aaaccacca aatgtcctc 
gtttacgcc gacagtttg gtcacgaaa gacaacgtc 
aaaagagtc ctattctca attttgaat aaggaaaag 
tacacaatc tacgacctc cccacttcg aaaaacacg 
ggttgggac aaatccaca gttctggtg gttgtgtgt 
ggtggtgtc ccacctgac ctcaccgcc aacctgatc 
caatatttc ctcaccgga ggtcaactc ctctgcatt 
tgcagcgac ctcatgtac agtgtactt tacacgttc 
acgacggcg gaagtacgc gagcacgaa ctcgtccgc 
ttctcgtac ggccaatgg cagcacgtt aaaatgatg 
catcatatt ttctgctat caagcatcg cctgctaag 
aaacagttc agtaaagat tcggatcat tcgagtaac 
ggctcaagt ccgcaagcg ccaaggacg ccctctaac 
gtcgaatta agacacaac gaaaaagat tataacatt 
caagttcaa gtgttaggg actgaagaa acgtggcat 
acgcccagc ctgctattg gctaaagtg aaaaactcg 
caagggagg gctgttttt tcgcaagtg catctggaa 
attgatcca gatgtttat gagaatgat gaggataag 
taccgggag ttgttgagt agtaatgaa gccagattg 
gagattttg aaagatatt atttccaat catttggat 
ttggtgtgt gatgagagt ttgggcacg gttgagtac 
agtcaaggg tattttctt gggcgttat gatgcaaaa 
atgaatatg atgaccgac tgtgactcc ataaccaat 
aaccaatta acttgtgat aaaatcagt gttgttttt 
tgtggccat aatgtcgag cctggcctt gcgtcaaat 
atccgcctt ccggtcatg attcattcg tgtccttcg 
aacttctcc actttggat tatttcgaa acgttgaac 
actgaagac attggccgt ttggtgatt tatgccgat 
attttgaca agtacccag cacctactt gacaaaaaa 
ttgcgccat ggtcttgtt gtgatcgcc cggcaacag 
acccatggc atgggacgg tcgagcaac tcatggttg 
agtccattg ggttgtgcg gccttttcc cttcagatg 
cacattccg ctggatagt cacatggga cagtcgctt 
cccttgatc cagcactta gtcatggta gctgtcata 
tcgtcgatt aaaaatgtc caaggtttg gattgtctc 
aatgttggt attaaatgg cctaatgat ttgtacgcg 
gaaggggcg attaaaatc ggtggactt gttgtaact 
tctacgata ttttcaaat tttgctatt gttaatata 
ggttgtggt gtaaattta tcaaaccaa aaccccaca 
atatgtata aatgatctc atagaaaaa aacacagtg 
atgcaacct atagcttat gagaaatat ttcgcagct 
gtgttcaat gaaatcgaa cgcttatac tttgatgtg 
cagaaaaat gggtttgag gcgtttttc cagctttat 
tacaagtat tggttgcac agcgatgcc gacgttacg 
atacagtcg gaaaagggc gattccaca cgagctaaa 
atcgtggga atcgacaaa tacggtttt ctctctgtc 
cgattggcc gacggcagt atgacgtca gtccagccc 
gacggtaat tcgttcgat ttgatgagc ggcttgata 
gcgccaaag aaattttaa
    mRNA sequence
    • >TC015419 RA
      gaattttta attattgtg gtcttgaga atttttttt 
aatgatagg tgcaatgga ggcataaaa cataatgat 
ctttacttt atattatgc ctacgccac cataatgca 
gtggtggcg tctccgaac tttacgcaa caaactcaa 
cggaacact aaacgatca aaatgcctt gcttgtctg 
ttctgggca ttcggtgca cgatagcag aaaacagcg 
aagtttgga gaatttatt gtacagtac tgagaatag 
gatcgcgtg cacagttgt gcctaagca aaaactaaa 
cttaagtca atggatcgc atttccgaa aaatctaag 
atacttccc gatttttat cgacgacaa taaaatcag 
tgttagtcg gccccaaat ttttctcct ccttcaggc 
cgttttaga caattatgt taaacatca gagtgagat 
tattcaagt tgagcattt tggcgaact gatcggttg 
gaaagcgag cgattcgtt cgaagttgt ggttaaaac 
agacatgga tcatttgac taagctggt caatcagtt 
tagccaaag cgatgttga tattaacca tgaattaaa 
gttacttaa aattgaaac tgttgatgt tgaaaatac 
acccactgt catcaaata cgagaaaaa atatctcaa 
agaacaaac tttgaaata ttcatatgc tccagttca 
ttgggggaa attcgtgca agaagtcaa ggacttgta 
ctcgaaaat acgcgacat taccactcc gaaaataaa 
aatcgaaaa tggttcgcc tcagtgcct cgaatccgc 
aaaacccat aataaaaaa agaaccaaa aaaaaccga 
tccactttc cgaagacaa aaacacact tgatgtgtc 
acgacgtca caaaaaacc acgtgaagg cagttcttc 
caaaccatc aaaaccatc aaagcacaa aacaaaaag 
cgacgaaaa acaacacaa acacaaacg aactgcaaa 
taaattagt caaacagag ctcagttaa agaaaacga 
agaggtcaa aagtgaagt caaagaaaa ggttataac 
agggggaac ccccaaacc accaaatgt cctcgttta 
cgccgacag tttggtcac gaaagacaa cgtcaaaag 
agtcctatt ctcaatttt gaataagga aaagtacac 
aatctacga cctccccac ttcgaaaaa cacgggttg 
ggacaaatc cacagttct ggtggttgt gtgtggtgg 
tgtcccacc tgacctcac cgccaacct gatccaata 
tttcctcac cggaggtca actcctctg catttgcag 
cgacctcat gtacagtgt actttacac gttcacgac 
ggcggaagt acgcgagca cgaactcgt ccgcttctc 
gtacggcca atggcagca cgttaaaat gatgcatca 
tattttctg ctatcaagc atcgcctgc taagaaaca 
gttcagtaa agattcgga tcattcgag taacggctc 
aagtccgca agcgccaag gacgccctc taacgtcga 
attaagaca caacgaaaa agattataa cattcaagt 
tcaagtgtt agggactga agaaacgtg gcatacgcc 
cagcctgct attggctaa agtgaaaaa ctcgcaagg 
gagggctgt tttttcgca agtgcatct ggaaattga 
tccagatgt ttatgagaa tgatgagga taagtaccg 
ggagttgtt gagtagtaa tgaagccag attggagat 
tttgaaaga tattatttc caatcattt ggatttggt 
gtgtgatga gagtttggg cacggttga gtacagtca 
agggtattt tcttgggcg ttatgatgc aaaaatgaa 
tatgatgac cgactgtga ctccataac caataacca 
attaacttg tgataaaat cagtgttgt tttttgtgg 
ccataatgt cgagcctgg ccttgcgtc aaatatccg 
ccttccggt catgattca ttcgtgtcc ttcgaactt 
ctccacttt ggattattt cgaaacgtt gaacactga 
agacattgg ccgtttggt gatttatgc cgatatttt 
gacaagtac ccagcacct acttgacaa aaaattgcg 
ccatggtct tgttgtgat cgcccggca acagaccca 
tggcatggg acggtcgag caactcatg gttgagtcc 
attgggttg tgcggcctt ttcccttca gatgcacat 
tccgctgga tagtcacat gggacagtc gcttccctt 
gatccagca cttagtcat ggtagctgt catatcgtc 
gattaaaaa tgtccaagg tttggattg tctcaatgt 
tggtattaa atggcctaa tgatttgta cgcggaagg 
ggcgattaa aatcggtgg acttgttgt aacttctac 
gatattttc aaattttgc tattgttaa tataggttg 
tggtgtaaa tttatcaaa ccaaaaccc cacaatatg 
tataaatga tctcataga aaaaaacac agtgatgca 
acctatagc ttatgagaa atatttcgc agctgtgtt 
caatgaaat cgaacgctt atactttga tgtgcagaa 
aaatgggtt tgaggcgtt tttccagct ttattacaa 
gtattggtt gcacagcga tgccgacgt tacgataca 
gtcggaaaa gggcgattc cacacgagc taaaatcgt 
gggaatcga caaatacgg ttttctctc tgtccgatt 
ggccgacgg cagtatgac gtcagtcca gcccgacgg 
taattcgtt cgatttgat gagcggctt gatagcgcc 
aaagaaatt ttaatcctt cggtgtttt ttctatttt 
tttagtatg atgtaatta ttggtcttt ttgatcttt 
gttgttgtc tcttggtgt acacgcaaa ttgtaagag 
gaaaaaaat ataaaatgt tgacgaaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY