Status
Please wait ...
Gene information
General information
TC006252 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5833474..5839139
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006252
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10632

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006252 RA
      MLRTTLLKA LTGSKLGPK HITVLSRAA SDVAKKPEK 
      IEVFIDDQP VLVEPGTTV LQAAALVGV EIPRFCYHE 
      RLAVAGNCR MCLVEVEKS PKPVAACAM PVMKGWRIK 
      TNSEMTKKA REGVMEFLL VNHPLDCPI CDQGGECDL 
      QDQSMAFGS DRSRFTDID FSGKRAVED KDIGPLIKT 
      IMTRCIHCT RCIRFASEI AGVDDLGTT GRGSGMEIG 
      TYVQKFFLS ELSGNVIDL CPVGALTSK PYSFTARPW 
      EIRKIDSID VLDAVGSNI VVSTRTGEV MRIMPRTNE 
      EINEEWLSD KARFAYDGL KRQRLVTPM LKATNGELK 
      PVDWETALL SVAKVMKNA GSSIGAIAG GLADAEALV 
      ALKDLLNRL GSENLCTEH TFPMDGSAT DLRSSYLLN 
      NKIMGAEEA DFALLIGTN PRFEAPLLN SRLRKAYVH 
      KELDVALIG PKVNLTYGY NHLGDDPEV ISKIASGSH 
      PIAQKLKSA KKPLIILGA DLLQRPDGA AILSQVQKI 
      ASLASPAEG WKVFNILHK VASQVAALD VGYTPGVDK 
      IRENNPKVI FLLGADECA IDRSDLPEG CFVVYQGHH 
      GDHGATIAD AVLPGAAYT EKQATYVNT EGRAQQTLP 
      AVSPPGNAR EDWKIIRAL SEILGAPLP YDSLEDIRC 
      RLHQVAPHL TRYGDAESA NYFKQAQEL AKNIQSDKI 
      SGSPIDVKI KELKDFYMT DAISRASPT MAKCVQAVL 
      KQEQSKY
    CDS sequence
    • >TC006252 RA
      atgctgcgt accaccctt ttaaaagcc ctcacgggg 
      tccaagctg ggccccaag cacatcacc gtcctgtct 
      cgggccgcg agtgatgtt gctaaaaag ccggaaaaa 
      atcgaagtc tttattgac gatcagcct gttttggtg 
      gaacctggg accaccgtc ctgcaagcc gcggctttg 
      gtcggtgtg gaaatcccc cgattttgc taccacgag 
      cgtctcgcg gttgctggt aactgccga atgtgtctc 
      gtggaagtg gaaaagtcc cccaaacct gtggcggct 
      tgtgccatg cctgttatg aaagggtgg cgcattaaa 
      accaactcc gagatgact aaaaaggcc cgtgagggg 
      gtcatggag ttccttttg gtgaaccac cccctcgat 
      tgccccatt tgcgaccaa gggggcgag tgtgatttg 
      caggaccag agtatggcg ttcgggtcc gataggtca 
      cggtttacc gatatagac ttctcgggg aagagggct 
      gtggaggat aaggatata gggcctttg attaagacg 
      ataatgacg aggtgtatt cactgcacg aggtgtatt 
      cgtttcgcg tccgaaatc gcaggggtt gatgatttg 
      gggacgacg ggacggggc tccggtatg gaaattggt 
      acttacgta cagaagttt ttcttgtcg gagttgtcg 
      ggaaatgtg attgatttg tgtcccgtg ggggcgctg 
      actagcaag ccgtatagt ttcacagcg aggccttgg 
      gagataagg aaaattgac tcgattgat gttttggat 
      gctgttggt tcgaatatt gtggtctcg acaaggacg 
      ggcgaggtg atgaggatt atgccgagg actaatgag 
      gaaatcaat gaggaatgg ttatcagat aaggcgcgg 
      tttgcgtac gacggcctc aaacgccaa cgcctcgtc 
      acccccatg ctgaaagcc acaaacggc gaactaaag 
      ccagtcgac tgggaaacg gctttactt tccgtcgcc 
      aaagtaatg aaaaacgcc ggctcaagc attggcgca 
      atcgccggc ggtttagcc gatgccgaa gcccttgta 
      gccctaaaa gacctcttg aaccgttta ggttcggaa 
      aatttgtgt acagaacac acattccca atggacggt 
      tccgccacc gatctccga tcgagttac ctcctcaac 
      aacaagatc atgggggct gaagaggcc gactttgcc 
      ctcctcatt ggaaccaat ccgcgattt gaagcccca 
      cttttaaac tcacgtttg aggaaagcc tacgtccac 
      aaagaactg gacgttgct ttaatcggc ccgaaagtc 
      aacctaacc tacggttat aaccacttg ggggatgac 
      ccggaggtt atttccaaa atcgcctca gggagccac 
      cccatcgcc cagaagtta aaatcggcg aaaaagcct 
      ctgattatc ctcggggcc gatttgttg cagcgcccc 
      gatggggct gccatttta tcccaagtg cagaaaatc 
      gcctcgttg gcgagccca gccgaaggc tggaaagtc 
      ttcaatatc ttgcacaag gttgccagt caggtcgca 
      gccctcgat gttggctac accccaggg gttgataaa 
      attcgcgaa aacaacccc aaagtcatt ttcctgttg 
      ggggccgac gagtgtgcg attgaccga agcgacttg 
      cctgagggt tgtttcgtg gtttatcaa gggcaccat 
      ggggaccat ggggctaca attgccgac gcggtgctc 
      ccgggggct gcctacacc gagaaacaa gcgacttat 
      gtgaacacc gaaggcagg gcgcagcag acgttgccg 
      gcggtctcg cccccagga aacgccagg gaggactgg 
      aagataatt agggcgctc tctgagatt ttaggggcc 
      cccttgccc tatgattcg ctggaggat atcaggtgt 
      aggttgcat caagtggcg ccccatttg acgagatat 
      ggggatgcc gaaagtgcg aactatttt aaacaagct 
      caagagttg gcaaagaat atacagagt gataaaata 
      agtgggtct ccgattgat gttaaaata aaggagttg 
      aaggacttt tatatgacg gatgcgata agtcgggct 
      tcgcccacc atggccaag tgtgtccaa gctgtgctg 
      aagcaggag cagtcaaaa tattga
    mRNA sequence
    • >TC006252 RA
      tgaaaacac aaaaataag aaaatgctg cgtaccacc 
      cttttaaaa gccctcacg gggtccaag ctgggcccc 
      aagcacatc accgtcctg tctcgggcc gcgagtgat 
      gttgctaaa aagccggaa aaaatcgaa gtctttatt 
      gacgatcag cctgttttg gtggaacct gggaccacc 
      gtcctgcaa gccgcggct ttggtcggt gtggaaatc 
      ccccgattt tgctaccac gagcgtctc gcggttgct 
      ggtaactgc cgaatgtgt ctcgtggaa gtggaaaag 
      tcccccaaa cctgtggcg gcttgtgcc atgcctgtt 
      atgaaaggg tggcgcatt aaaaccaac tccgagatg 
      actaaaaag gcccgtgag ggggtcatg gagttcctt 
      ttggtgaac caccccctc gattgcccc atttgcgac 
      caagggggc gagtgtgat ttgcaggac cagagtatg 
      gcgttcggg tccgatagg tcacggttt accgatata 
      gacttctcg gggaagagg gctgtggag gataaggat 
      atagggcct ttgattaag acgataatg acgaggtgt 
      attcactgc acgaggtgt attcgtttc gcgtccgaa 
      atcgcaggg gttgatgat ttggggacg acgggacgg 
      ggctccggt atggaaatt ggtacttac gtacagaag 
      tttttcttg tcggagttg tcgggaaat gtgattgat 
      ttgtgtccc gtgggggcg ctgactagc aagccgtat 
      agtttcaca gcgaggcct tgggagata aggaaaatt 
      gactcgatt gatgttttg gatgctgtt ggttcgaat 
      attgtggtc tcgacaagg acgggcgag gtgatgagg 
      attatgccg aggactaat gaggaaatc aatgaggaa 
      tggttatca gataaggcg cggtttgcg tacgacggc 
      ctcaaacgc caacgcctc gtcaccccc atgctgaaa 
      gccacaaac ggcgaacta aagccagtc gactgggaa 
      acggcttta ctttccgtc gccaaagta atgaaaaac 
      gccggctca agcattggc gcaatcgcc ggcggttta 
      gccgatgcc gaagccctt gtagcccta aaagacctc 
      ttgaaccgt ttaggttcg gaaaatttg tgtacagaa 
      cacacattc ccaatggac ggttccgcc accgatctc 
      cgatcgagt tacctcctc aacaacaag atcatgggg 
      gctgaagag gccgacttt gccctcctc attggaacc 
      aatccgcga tttgaagcc ccactttta aactcacgt 
      ttgaggaaa gcctacgtc cacaaagaa ctggacgtt 
      gctttaatc ggcccgaaa gtcaaccta acctacggt 
      tataaccac ttgggggat gacccggag gttatttcc 
      aaaatcgcc tcagggagc caccccatc gcccagaag 
      ttaaaatcg gcgaaaaag cctctgatt atcctcggg 
      gccgatttg ttgcagcgc cccgatggg gctgccatt 
      ttatcccaa gtgcagaaa atcgcctcg ttggcgagc 
      ccagccgaa ggctggaaa gtcttcaat atcttgcac 
      aaggttgcc agtcaggtc gcagccctc gatgttggc 
      tacacccca ggggttgat aaaattcgc gaaaacaac 
      cccaaagtc attttcctg ttgggggcc gacgagtgt 
      gcgattgac cgaagcgac ttgcctgag ggttgtttc 
      gtggtttat caagggcac catggggac catggggct 
      acaattgcc gacgcggtg ctcccgggg gctgcctac 
      accgagaaa caagcgact tatgtgaac accgaaggc 
      agggcgcag cagacgttg ccggcggtc tcgccccca 
      ggaaacgcc agggaggac tggaagata attagggcg 
      ctctctgag attttaggg gcccccttg ccctatgat 
      tcgctggag gatatcagg tgtaggttg catcaagtg 
      gcgccccat ttgacgaga tatggggat gccgaaagt 
      gcgaactat tttaaacaa gctcaagag ttggcaaag 
      aatatacag agtgataaa ataagtggg tctccgatt 
      gatgttaaa ataaaggag ttgaaggac ttttatatg 
      acggatgcg ataagtcgg gcttcgccc accatggcc 
      aagtgtgtc caagctgtg ctgaagcag gagcagtca 
      aaatattga atagtttag ataattgtg tattataaa 
      agaatcaat aaaataata aacgaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY