Status
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Gene information
General information
TC000818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6218836..6225096
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000818
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10631

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000818 RA
      MKETEDNNV FVLSKRLDD AEKNKNSDE CRKPADFET 
      AIGATGFGK FNIILLLIA MPAGWSSIF ETTTMSYVF 
      PAAQCDLSL TLENKGFLN AITYIGMIS SAFVWGYLC 
      DTLGRKKLL AIGLFLDGI FVLMSASSQ NATILMIAK 
      FLGGFIING PFAALTTYL SEFHCARHR ARIQMMLGI 
      VFSLGTVIL PFVAWAVLP LNLSVKIFN DSVEFHSWN 
      LYLCLCAFP ALISSIAFI FMPESPKFL MSMGRNEKA 
      LEVFRKVYC FNTGNAPET FPIKELVEE TKLNNDSKH 
      GGRITANRT KLQAMKEGW QQITPLFFP PHLGKIILV 
      CLLQMLTMM SLNTLRLWL PQIFQAIND YQYYHNGST 
      TDLCTMIDQ IRPKNQSQN CEVNLNNSS VYINSIIVA 
      VVSIGGYII AGMLINALG KKRLLNILG ILSGSGAMC 
      LYFAQNSLS TIILSCLFV SLGSININV VLAVVVDLF 
      PTTLRTMTI SLTMMVGRS GAMLGNLIF PVLLQLGCA 
      PPFFTVGAA IVGCAFLAC LLPNTDLKA LQ
    CDS sequence
    • >TC000818 RA
      atgaaggaa accgaagat aacaacgta tttgtgtta 
      tcaaaaaga ctggatgac gctgagaag aacaagaac 
      tcggacgaa tgtcggaaa ccggccgat ttcgagacg 
      gccatagga gccactggt ttcggtaaa ttcaacata 
      atcctcctc ttgatcgct atgccagca ggctggagc 
      tcaatcttc gagaccaca accatgtcg tacgttttt 
      ccagcagct caatgcgac ttgagtttg acgctggaa 
      aataaagga tttcttaac gctattact tacatcgga 
      atgatatcg agtgcgttc gtttggggg tatttgtgc 
      gacacccta ggccggaaa aaactcctg gccattggc 
      ttattcctt gacggaatt ttcgttcta atgagcgca 
      tcgtcgcaa aacgcaact attcttatg atcgccaag 
      tttttggga ggtttcata ataaatgga ccttttgcg 
      gcacttacg acttactta tcagagttc cactgtgcc 
      agacacagg gctcggatc cagatgatg ctagggatt 
      gtctttagt ttagggact gtgatattg ccctttgtg 
      gcctgggcg gttcttccg cttaatcta agtgtaaag 
      atttttaac gatagtgta gaatttcac tcgtggaat 
      ttatacttg tgtttgtgt gcatttccg gctttgatt 
      agttcgatt gcttttatt ttcatgccg gaaagtccg 
      aaattcttg atgtcgatg ggccggaat gaaaaggct 
      ttggaggtt ttcaggaaa gtttactgt tttaataca 
      ggaaatgcg cccgaaacg tttccgatc aaagaactg 
      gtcgaggaa acaaaactg aataatgac tcgaaacac 
      gggggcaga ataacggcc aacagaacc aagctacaa 
      gcgatgaaa gaagggtgg cagcagatc acgcctctg 
      tttttccca cctcattta ggcaaaatc attttagtt 
      tgtttgcta caaatgctg acaatgatg agtcttaac 
      acgttgcgc ctttggctg ccacaaatc ttccaagcg 
      atcaacgat tatcaatat tatcacaat ggatcgacc 
      actgatctt tgcacaatg atcgaccaa atacggcct 
      aagaaccaa tctcaaaat tgcgaagtg aatttgaac 
      aactcgtcg gtttacatc aactcaatc atcgttgct 
      gttgtctcg attggtggc tacatcatt gcagggatg 
      ctaatcaat gcgttgggc aaaaaaaga cttctcaac 
      atcctggga atactttct ggatctggt gcgatgtgt 
      ttgtacttt gctcaaaac tcgctatca acaataatt 
      ttatcgtgt ctttttgtc tccctgggc agtattaac 
      ataaacgta gtccttgcc gtcgttgtt gatcttttc 
      ccaacaact ttacgcaca atgaccatc tccctaacc 
      atgatggtt gggcggtcg ggtgccatg ttgggtaat 
      ttgatattt ccagtttta ctacaatta gggtgtgca 
      ccacccttt ttcactgtg ggggctgca attgttggc 
      tgtgctttc ttagcgtgt cttttacca aatacggac 
      ctgaaagcg ctacagtag
    mRNA sequence
    • >TC000818 RA
      gctgtcggc cgtgaacaa gcatgtgtt tagtttaaa 
      atagctttt ttggcagag cgtttgaga ggagacgag 
      tctgcgtca tcgatcaac cactgcttt attaacact 
      ttgagattc tcatcagat aagttaagt ccgggctca 
      aactcgtgt aaataagag aaaattgtt ttcgctaac 
      gttaacaca actgtgaca ttcaaacca tgaaggaaa 
      ccgaagata acaacgtat ttgtgttat caaaaagac 
      tggatgacg ctgagaaga acaagaact cggacgaat 
      gtcggaaac cggccgatt tcgagacgg ccataggag 
      ccactggtt tcggtaaat tcaacataa tcctcctct 
      tgatcgcta tgccagcag gctggagct caatcttcg 
      agaccacaa ccatgtcgt acgtttttc cagcagctc 
      aatgcgact tgagtttga cgctggaaa ataaaggat 
      ttcttaacg ctattactt acatcggaa tgatatcga 
      gtgcgttcg tttgggggt atttgtgcg acaccctag 
      gccggaaaa aactcctgg ccattggct tattccttg 
      acggaattt tcgttctaa tgagcgcat cgtcgcaaa 
      acgcaacta ttcttatga tcgccaagt ttttgggag 
      gtttcataa taaatggac cttttgcgg cacttacga 
      cttacttat cagagttcc actgtgcca gacacaggg 
      ctcggatcc agatgatgc tagggattg tctttagtt 
      tagggactg tgatattgc cctttgtgg cctgggcgg 
      ttcttccgc ttaatctaa gtgtaaaga tttttaacg 
      atagtgtag aatttcact cgtggaatt tatacttgt 
      gtttgtgtg catttccgg ctttgatta gttcgattg 
      cttttattt tcatgccgg aaagtccga aattcttga 
      tgtcgatgg gccggaatg aaaaggctt tggaggttt 
      tcaggaaag tttactgtt ttaatacag gaaatgcgc 
      ccgaaacgt ttccgatca aagaactgg tcgaggaaa 
      caaaactga ataatgact cgaaacacg ggggcagaa 
      taacggcca acagaacca agctacaag cgatgaaag 
      aagggtggc agcagatca cgcctctgt ttttcccac 
      ctcatttag gcaaaatca ttttagttt gtttgctac 
      aaatgctga caatgatga gtcttaaca cgttgcgcc 
      tttggctgc cacaaatct tccaagcga tcaacgatt 
      atcaatatt atcacaatg gatcgacca ctgatcttt 
      gcacaatga tcgaccaaa tacggccta agaaccaat 
      ctcaaaatt gcgaagtga atttgaaca actcgtcgg 
      tttacatca actcaatca tcgttgctg ttgtctcga 
      ttggtggct acatcattg cagggatgc taatcaatg 
      cgttgggca aaaaaagac ttctcaaca tcctgggaa 
      tactttctg gatctggtg cgatgtgtt tgtactttg 
      ctcaaaact cgctatcaa caataattt tatcgtgtc 
      tttttgtct ccctgggca gtattaaca taaacgtag 
      tccttgccg tcgttgttg atcttttcc caacaactt 
      tacgcacaa tgaccatct ccctaacca tgatggttg 
      ggcggtcgg gtgccatgt tgggtaatt tgatatttc 
      cagttttac tacaattag ggtgtgcac caccctttt 
      tcactgtgg gggctgcaa ttgttggct gtgctttct 
      tagcgtgtc ttttaccaa atacggacc tgaaagcgc 
      tacagtagt tgtaacttg taacttata acgaactgt 
      ggtaccaat ttttttagc aatgtgtta cttgttgaa 
      attaataaa aaatacaac attaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY