Status
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Gene information
General information
TC012454 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7734736..7756143
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012454
    iBeetle screen iB_10628

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012454 RA
      MSVWETVPD ELSYGIAIY NFAADSDFK LELTVGDSL 
      HILDQEENW YYGYLIHDR HKRGIFPKS YVHIKPCSV 
      DRTGPTLTF NFKEPTIVQ EITSVLREW APHWKNLYV 
      KNSKHFEAI KNQIYDLIT HRSKIISGT LPVDELKRI 
      TKQVTEEID KGNKTLRLD LIVRNKNGN VIEPEKTST 
      LQMYYLHKK ATERMSRTN KNETKHNHP KTAIQQYSN 
      IFIVAVKNF TCKMNEDAE LLMSLYDGK EYKSITENY 
      VVRWSKDGL MCDLDQMYN LRVMFTDLG KRDLEREKI 
      YLVCYVVRV GAMESKEPD HRRSSVSVV NKKGTTDGI 
      RRPFGVAAM EITHFMNGS RESDLEQEF PVPFFNCDK 
      DNLEQTLKK IINKDFAKT EHKNHALFV GMKLLRGDL 
      KQVREENPH LVLGNLSIA RKMGFPEVI LPGDVRNDL 
      YLTLIKGEF SKGSKTCDK NVEVVVRVC NEEGVPIPG 
      VIALGSGVP PIDEYKSMI YYHEDKPHW YETFKVAIP 
      IEEFKTSHL KFTFKHRSS NEAKDKNEK PFAMSYVRL 
      MQENGTTLK DARHNLIVY KIDYKKFDE KGLDYFKLP 
      SIVSDVKDN AKPSVAGLT MSKDIFVMS SNICSTKLT 
      QNVDLLGLL NWKTHKDDL KSCLRALIK VDGEEVVKL 
      LQDTLDSLF NILIEFTET DTYDIYVFD CLLHIISLV 
      SNDWKYQHF EPVLDLYIK ENFSATLAH KKLISVLKN 
      IIGRANSKT PDSKDDLIF KTMKSLQYV MRFVSKSRI 
      LMKVVDDVE DDFEESLRD LLQDITDMM ALTSDGILR 
      EQGACLKYL PSTIPDILM IFDAKELSV ILCNIITNI 
      PPGRLTKQK MMTINEIVH SKLFLYTEC RRIMLPIFT 
      KQVKTLFEL HEEVELCIK ILSDILELL FRKDIGPTY 
      DDINEIITT VLRTLIHTR VGMPKEDPQ LGNLVALMI 
      DIFRQMSER HYEAYIERF NTRFDVLDF LMEILLFFQ 
      NLVDDKVFP YDWCDMIML QNSVILKAL RFFSHTIRD 
      RFFERFEHQ AWNNFFHCA IAFMTQPAL QLETFSFSK 
      RMKIIRIYK DMRRETGFE IRSMWFNLG QYKVQFVPS 
      LVGAILEMT LIPEPELRK ATIPIFFDM MQCEFYSSR 
      FELESYGDT KRDSSHIKG NFNDFENEM IAKLDILVE 
      GGKGDENYK DLFHDIMME HCSQHATMN ETGIKFVKT 
      VTRLMERLL EYRCIITDE NKENRMSCT VSLLDFYAE 
      INRKEMYIR YLNKLYDLH LECDNYTEA AYTIDLHAK 
      LLNWTDEDL PQLLKTNRH PKAHTHRQL KEALYYNII 
      ENYDRGKLW ECAIKKCQE LASQFESET YDYHRLSQL 
      HKRMSTFYD DIMKKGRAK PEYFRVGYF GKGFPQFLQ 
      NKVFVYRGK EYERLTEFN TRILNEFPK AELLNKLTP 
      PGEDITESD KQYVQINKV DPVMDEKKH RFSGKPVSE 
      QILNYYKVN DIQKFTFSR PFIRKDPYI DDKNEFGHL 
      WLERTELTT TYPLPGILR WFPVSSSTV HEISPLRNA 
      IETMEKANK TLKNYVVLF NNDKDMQIN PLSLTLNGI 
      LDAAVMGGI KNYEEAFFT EKYETHHQE DDVLLQRLK 
      DLIADQIPL LELCVRIHK ENAPENLQP LQKRFEDCF 
      AKMQESVEE KYGKGTCDI KLDSQVTMR RHHFVLPDP 
      RLSDMSMLS DKSSISSNT KSLLVSPSK SLTTTPALN 
      HKKSFKITR EKRRSSKSD HTPSPSLLP ATQWYTTDE 
      NTNGSPIFE LTEELTPKR PLRSEVEKE KRLSRPPSG 
      QFSSRSSSI SVTLRGTGS SSSSNRDSV GTTDSSVSE 
      EDAVPPPLP LKTREVDYC NLPNNENTS FLYSQRNSV 
      RTSLKIQVQ AADPISFDD VPPTPPPKP PKNKSISNL
    • >TC012454 RB
      MSVWETVPD ELSYGIAIY NFAADSDFK LELTVGDSL 
      HILDQEENW YYGYLIHDR HKRGIFPKS YVHIKPCSV 
      DRTGPTLTF NFKEPTIVQ EITSVLREW APHWKNLYV 
      KNSKHFEAI KNQIYDLIT HRSKIISGT LPVDELKRI 
      TKQVTEEID KGNKTLRLD LIVRNKNGN VIEPEKTST 
      LQMYYLHKK ATERMSRTN KNETKHNHP KTAIQQYSN 
      IFIVAVKNF TCKMNEDAE LLMSLYDGK EYKSITENY 
      VVRWSKDGL MCDLDQMYN LRVMFTDLG KRDLEREKI 
      YLVCYVVRV GAMESKEPD HRRSSVSVV NKKGTTDGI 
      RRPFGVAAM EITHFMNGS RESDLEQEF PVPFFNCDK 
      DNLEQTLKK IINKDFAKT EHKNHALFV GMKLLRGDL 
      KQVREENPH LVLGNLSIA RKMGFPEVI LPGDVRNDL 
      YLTLIKGEF SKGSKTCDK NVEVVVRVC NEEGVPIPG 
      VIALGSGVP PIDEYKSMI YYHEDKPHW YETFKVAIP 
      IEEFKTSHL KFTFKHRSS NEAKDKNEK PFAMSYVRL 
      MQENGTTLK DARHNLIVY KIDYKKFDE KGLDYFKLP 
      SIVSDVKDN AKPSVAGLT MSKDIFVMS SNICSTKLT 
      QNVDLLGLL NWKTHKDDL KSCLRALIK VDGEEVVKL 
      LQDTLDSLF NILIEFTET DTYDIYVFD CLLHIISLV 
      SNDWKYQHF EPVLDLYIK ENFSATLAH KKLISVLKN 
      IIGRANSKT PDSKDDLIF KTMKSLQYV MRFVSKSRI 
      LMKVVDDVE DDFEESLRD LLQDITDMM ALTSDGILR 
      EQGACLKYL PSTIPDILM IFDAKELSV ILCNIITNI 
      PPGRLTKQK MMTINEIVH SKLFLYTEC RRIMLPIFT 
      KQVKTLFEL HEEVELCIK ILSDILELL FRKDIGPTY 
      DDINEIITT VLRTLIHTR VGMPKEDPQ LGNLVALMI 
      DIFRQMSER HYEAYIERF NTRFDVLDF LMEILLFFQ 
      NLVDDKVFP YDWCDMIML QNSVILKAL RFFSHTIRD 
      RFFERFEHQ AWNNFFHCA IAFMTQPAL QLETFSFSK 
      RMKIIRIYK DMRRETGFE IRSMWFNLG QYKVQFVPS 
      LVGAILEMT LIPEPELRK ATIPIFFDM MQCEFYSSR 
      FELESYGDT KRDSSHIKG NFNDFENEM IAKLDILVE 
      GGKGDENYK DLFHDIMME HCSQHATMN ETGIKFVKT 
      VTRLMERLL EYRCIITDE NKENRMSCT VSLLDFYAE 
      INRKEMYIR YLNKLYDLH LECDNYTEA AYTIDLHAK 
      LLNWTDEDL PQLLKTNRH PKAHTHRQL KEALYYNII 
      ENYDRGKLW ECAIKKCQE LASQFESET YDYHRLSQL 
      HKRMSTFYD DIMKKGRAK PEYFRVGYF GKGFPQFLQ 
      NKVFVYRGK EYERLTEFN TRILNEFPK AELLNKLTP 
      PGEDITESD KQYVQINKV DPVMDEKKH RFSGKPVSE 
      QILNYYKVN DIQKFTFSR PFIRKDPYI DDKNEFGHL 
      WLERTELTT TYPLPGILR WFPVSSSTV HEISPLRNA 
      IETMEKANK TLKNYVVLF NNDKDMQIN PLSLTLNGI 
      LDAAVMGGI KNYEEAFFT EKYETHHQE DDVLLQRLK 
      DLIADQIPL LELCVRIHK ENAPENLQP LQKRFEDCF 
      AKMQESVEE KYGKGTCDI KLDSQVTMR RHHFVLPDP 
      RLSDMSMLS DSVDSRSRV SSLTKSQVA SLKQFTSSF 
      NFATSPSIS SKSSISSNT KSLLVSPSK SLTTTPALN 
      HKKSFKITR EKRRSSKSD HTPSPSLLP ATQWYTTDE 
      NTNGSPIFE LTEELTPKR PLRSEVEKE KRLSRPPSG 
      QFSSRSSSI SVTLRGTGS SSSSNRDSV GTTDSSVSE 
      EDAVPPPLP LKTREVDYC NLPNNENTS FLYSQRNSV 
      RTSLKIQVQ AADPISFDD VPPTPPPKP PKNKSISNL
    CDS sequence
    • >TC012454 RA
      atgagtgtg tgggaaaca gttcctgac gagctcagc 
      tacggcata gcaatatac aatttcgcc gccgacagc 
      gacttcaaa ctagaactg accgtcggg gattccctc 
      catatactc gaccaagaa gaaaactgg tactacggc 
      tacctgatt cacgacaga cacaaacga ggcattttc 
      cccaagagt tacgttcac attaaacca tgcagtgtt 
      gaccgcacc ggacctacc ctaacgttc aatttcaaa 
      gagccaaca atcgtgcaa gagataacg tcggtgttg 
      cgagaatgg gccccgcat tggaaaaac ctctacgta 
      aagaatagc aaacatttc gaagccatc aaaaatcag 
      atttacgat ctgataacg cacaggagc aaaatcatc 
      agcggtact ttaccggtc gacgaattg aaacggata 
      acgaaacaa gtcactgag gagatcgat aaggggaat 
      aaaacttta cgtttagat ttaattgtg agaaacaaa 
      aacggaaat gtcatcgaa ccggaaaag accagcacg 
      ctgcaaatg tactatctg cataaaaaa gcgacggaa 
      cggatgagt aggaccaat aagaacgaa accaaacac 
      aaccatccc aaaactgcc attcaacaa tactccaat 
      atttttata gttgccgtt aaaaatttc acctgtaag 
      atgaatgag gatgccgag cttttaatg tcgctttac 
      gacggaaaa gagtacaaa tctatcaca gaaaattat 
      gtcgtccgg tggtctaaa gatggactg atgtgcgac 
      ttggaccag atgtacaat ttgagagtt atgtttacg 
      gatctcggg aaacgcgat ttagagcgg gaaaaaatt 
      tacttggtt tgctacgtg gtccgcgtg ggtgcgatg 
      gaatcaaag gagcccgac catcgccgg agttccgtt 
      tcagtagtg aacaaaaaa ggcacaacc gacggaatc 
      cgtcgacct ttcggtgtc gcagcgatg gaaatcact 
      catttcatg aacgggtcg cgagagagc gacttggaa 
      caagaattt cccgttcct tttttcaac tgcgataag 
      gacaatctc gagcaaact ttaaagaag ataattaac 
      aaagacttc gccaaaacg gagcataaa aatcacgct 
      cttttcgtc ggaatgaag ttactgcgg ggcgattta 
      aagcaggtg cgggaggag aacccccat ctggtgctg 
      ggaaatttg tcgatcgcc cgaaaaatg ggcttcccg 
      gaagttatt ttaccggga gacgtccgg aacgatctt 
      tatttgact ttgattaaa ggagagttc agcaaaggg 
      agcaaaact tgcgataaa aacgtcgaa gttgtcgtc 
      agagtttgc aacgaagag ggagtcccg atccctggg 
      gttatcgcg ttgggttcg ggggttccg cccattgac 
      gaatacaaa tccatgatt tattatcac gaggataaa 
      ccgcactgg tatgaaact ttcaaagtc gcgattcct 
      attgaagaa ttcaagacg agtcatttg aaattcact 
      ttcaaacac cggtcttcc aatgaggcg aaagacaaa 
      aacgagaag ccgttcgcc atgtcctat gtgcgcctc 
      atgcaggag aatggcacg actctcaaa gacgcgcgc 
      cataatttg attgtctac aaaatcgat tacaaaaaa 
      ttcgatgaa aaaggcctg gattacttc aaattaccc 
      tcgattgtt agcgacgtc aaggacaat gccaagcca 
      tcagtcgcc ggactgacg atgtccaag gacattttc 
      gtgatgtcg agcaatatt tgctcgacg aaactgaca 
      cagaatgtg gatttgttg ggtcttttg aactggaaa 
      acgcacaag gatgactta aaatcgtgt cttcgagcc 
      ttgatcaaa gtagacggc gaagaagtc gtcaaattg 
      ttgcaggat actttagat tcgcttttt aacattttg 
      atagagttt accgaaacc gatacgtac gatatttac 
      gtttttgac tgtttgttg cacataatt agtctagtc 
      agtaacgac tggaagtac cagcacttc gagccggtc 
      ctggatttg tacattaag gagaatttc agtgcgact 
      ttggcccat aagaaattg atcagcgtc ctcaagaac 
      ataatcggg cgcgccaac agcaaaacg cccgatagc 
      aaagacgat ctcattttt aaaacaatg aagtcgctc 
      caatacgtg atgagattc gtctcgaaa tcgcgcatt 
      ttgatgaaa gtggtcgac gatgtcgag gacgatttc 
      gaggaaagt ctccgcgat ttgctgcaa gacatcacc 
      gacatgatg gccctcaca tcggacggg atcttgcgg 
      gagcaaggc gcctgcctt aagtacctc cccagcacg 
      attcccgac atcctgatg atttttgat gcgaaagag 
      ctgagcgtg attctgtgc aatatcatc acgaacatc 
      cctcccggc cgtctcacg aagcaaaaa atgatgaca 
      atcaacgaa atcgtccac agtaaactg tttctctac 
      acggaatgt cgcaggata atgctgccg atcttcacc 
      aagcaagtc aagaccctg ttcgagctg cacgaggag 
      gtcgagttg tgtataaaa atcctgagc gatattttg 
      gagctgctg ttccgtaag gacatcgga cccacgtat 
      gacgacatc aatgagatt ataaccacg gttttgcgg 
      actttaatt cacactcga gttggcatg cctaaggag 
      gatcctcaa ttggggaac ttggtcgcg ctaatgatc 
      gatattttc cggcaaatg tcggagcgc cactatgag 
      gcctacatc gagcgcttc aacacgcgt ttcgacgtt 
      ttagatttt ttgatggag attttgttg tttttccaa 
      aatttggtc gacgataag gtctttccg tacgactgg 
      tgcgacatg ataatgctc caaaacagc gttattttg 
      aaagctttg cggtttttc tctcatacg attcgtgac 
      cgtttcttc gaaagattc gaacatcaa gcctggaac 
      aacttcttt cactgtgct atcgctttc atgacccag 
      ccggctctc caactcgaa actttctcg ttcagtaaa 
      cggatgaaa atcatccga atctataaa gatatgcgg 
      cgcgagacc ggcttcgaa atcaggtca atgtggttc 
      aatttggga cagtataaa gtacagttc gttccgagt 
      ttggttggg gcgattcta gaaatgaca ctcataccg 
      gaaccggaa ttgcgtaaa gcaacgatt ccgattttc 
      ttcgatatg atgcagtgc gagttttac tcgtcgagg 
      ttcgaactc gagagctat ggcgatacg aaacgagat 
      tcgtcgcat atcaagggg aattttaac gatttcgag 
      aatgaaatg atcgcgaaa ttggatatt ctagtcgag 
      ggagggaaa ggagatgag aattacaaa gatttgttt 
      catgatatt atgatggag cattgcagc caacatgcc 
      acgatgaac gaaacgggg attaagttt gttaaaact 
      gtaacgcgg ttgatggaa cgcttgctc gaataccgg 
      tgtattatt accgatgag aacaaggaa aatcgcatg 
      agttgcact gtgagcctt ttggacttt tatgccgaa 
      atcaaccga aaagaaatg tacattcgc tacttgaat 
      aaactgtac gacctccat ttggagtgt gataattac 
      accgaagcc gcctacact atcgacttg cacgcgaaa 
      cttttaaat tggaccgac gaggattta ccccaactt 
      ttgaaaact aatcgacac cctaaagct cacacgcac 
      cgccaactc aaggaagct ctctattat aacattatc 
      gagaattac gacaggggc aaattatgg gagtgtgcg 
      atcaaaaag tgccaagaa ctggcctcg caattcgag 
      agcgaaacg tacgattac caccgactg agccagttg 
      cacaaaaga atgtcgacc ttttacgat gacattatg 
      aaaaaaggt cgcgcaaaa ccggagtac ttccgtgtc 
      gggtatttc ggcaagggc ttcccccaa tttctgcaa 
      aataaagtg tttgtatat cgggggaag gaatacgaa 
      cgacttacc gaattcaat acgagaata ttgaacgag 
      ttcccgaaa gcggaactt ttgaataaa ttgacgccg 
      ccgggggag gatatcacc gaatcggat aaacaatac 
      gtacaaata aataaagtt gatccggtc atggacgag 
      aaaaagcac cggttttcc ggcaaaccc gtctccgaa 
      caaattctc aattactac aaagtgaac gacatacag 
      aaatttacg ttttcgcgc ccgtttatc cgcaaggac 
      ccgtacatt gacgacaag aacgagttc ggtcacttg 
      tggctagag cggaccgaa ctgacaacg acgtaccct 
      ttgcccggg attttgcgc tggttccct gtgagctca 
      tcaacggtc catgagata tctcctctg cggaacgcc 
      atcgaaacg atggaaaaa gccaacaaa acgttgaag 
      aactacgtg gttttgttc aacaacgat aaggacatg 
      cagattaat ccgttgagc ttgactctg aacggcatc 
      ctggacgcc gccgtcatg ggaggcata aaaaattac 
      gaagaggcg ttctttacg gaaaaatac gagacgcat 
      catcaggaa gatgatgtg ctgttgcaa agactgaag 
      gatctgatc gctgatcag ataccgctg ctggagttg 
      tgcgtcaga atacacaag gaaaatgcc cctgaaaac 
      ttgcagccc ctgcagaag cggttcgag gactgtttt 
      gcgaaaatg caagagagt gtcgaggag aagtacgga 
      aaggggacc tgtgatatt aagttagat agtcaagta 
      acgatgagg aggcaccac tttgttctt cctgacccg 
      cgcttgtcc gacatgtcg atgctttca gacaaatct 
      agcatcagt agcaacacc aaaagttta ttagtgtcc 
      ccatcgaaa agtctaaca acaactccg gccttaaat 
      cacaaaaag tcgttcaaa atcacccga gaaaaacgc 
      cgttcgagt aagtctgat cacacgccg agtccgtcc 
      ctacttccg gccactcag tggtacacc actgacgaa 
      aatacaaac ggatcacct atatttgaa ctcactgaa 
      gagttgacc ccaaaacgg cccttgcgc tccgaagtc 
      gagaaggag aaacgcttg agccggccc ccaagtggt 
      cagttttct tccaggtct agcagtatt tcagtgacg 
      ttaagagga accggaagt tcgagtagc agtaaccgg 
      gattcggtt ggtacgact gactcaagt gtgagtgag 
      gaagacgcg gtgccgccc cctttaccg ttaaaaaca 
      agagaagtc gattactgt aatttgccg aataacgaa 
      aacaccagt tttctttat tcacaacgt aattcagtc 
      aggactagt ttgaaaatt caggttcag gcggctgat 
      ccgataagt tttgatgac gtaccgccc acgccgccg 
      ccaaaaccg cccaaaaac aaaagcata tccaattta 
      tga
    • >TC012454 RB
      atgagtgtg tgggaaaca gttcctgac gagctcagc 
      tacggcata gcaatatac aatttcgcc gccgacagc 
      gacttcaaa ctagaactg accgtcggg gattccctc 
      catatactc gaccaagaa gaaaactgg tactacggc 
      tacctgatt cacgacaga cacaaacga ggcattttc 
      cccaagagt tacgttcac attaaacca tgcagtgtt 
      gaccgcacc ggacctacc ctaacgttc aatttcaaa 
      gagccaaca atcgtgcaa gagataacg tcggtgttg 
      cgagaatgg gccccgcat tggaaaaac ctctacgta 
      aagaatagc aaacatttc gaagccatc aaaaatcag 
      atttacgat ctgataacg cacaggagc aaaatcatc 
      agcggtact ttaccggtc gacgaattg aaacggata 
      acgaaacaa gtcactgag gagatcgat aaggggaat 
      aaaacttta cgtttagat ttaattgtg agaaacaaa 
      aacggaaat gtcatcgaa ccggaaaag accagcacg 
      ctgcaaatg tactatctg cataaaaaa gcgacggaa 
      cggatgagt aggaccaat aagaacgaa accaaacac 
      aaccatccc aaaactgcc attcaacaa tactccaat 
      atttttata gttgccgtt aaaaatttc acctgtaag 
      atgaatgag gatgccgag cttttaatg tcgctttac 
      gacggaaaa gagtacaaa tctatcaca gaaaattat 
      gtcgtccgg tggtctaaa gatggactg atgtgcgac 
      ttggaccag atgtacaat ttgagagtt atgtttacg 
      gatctcggg aaacgcgat ttagagcgg gaaaaaatt 
      tacttggtt tgctacgtg gtccgcgtg ggtgcgatg 
      gaatcaaag gagcccgac catcgccgg agttccgtt 
      tcagtagtg aacaaaaaa ggcacaacc gacggaatc 
      cgtcgacct ttcggtgtc gcagcgatg gaaatcact 
      catttcatg aacgggtcg cgagagagc gacttggaa 
      caagaattt cccgttcct tttttcaac tgcgataag 
      gacaatctc gagcaaact ttaaagaag ataattaac 
      aaagacttc gccaaaacg gagcataaa aatcacgct 
      cttttcgtc ggaatgaag ttactgcgg ggcgattta 
      aagcaggtg cgggaggag aacccccat ctggtgctg 
      ggaaatttg tcgatcgcc cgaaaaatg ggcttcccg 
      gaagttatt ttaccggga gacgtccgg aacgatctt 
      tatttgact ttgattaaa ggagagttc agcaaaggg 
      agcaaaact tgcgataaa aacgtcgaa gttgtcgtc 
      agagtttgc aacgaagag ggagtcccg atccctggg 
      gttatcgcg ttgggttcg ggggttccg cccattgac 
      gaatacaaa tccatgatt tattatcac gaggataaa 
      ccgcactgg tatgaaact ttcaaagtc gcgattcct 
      attgaagaa ttcaagacg agtcatttg aaattcact 
      ttcaaacac cggtcttcc aatgaggcg aaagacaaa 
      aacgagaag ccgttcgcc atgtcctat gtgcgcctc 
      atgcaggag aatggcacg actctcaaa gacgcgcgc 
      cataatttg attgtctac aaaatcgat tacaaaaaa 
      ttcgatgaa aaaggcctg gattacttc aaattaccc 
      tcgattgtt agcgacgtc aaggacaat gccaagcca 
      tcagtcgcc ggactgacg atgtccaag gacattttc 
      gtgatgtcg agcaatatt tgctcgacg aaactgaca 
      cagaatgtg gatttgttg ggtcttttg aactggaaa 
      acgcacaag gatgactta aaatcgtgt cttcgagcc 
      ttgatcaaa gtagacggc gaagaagtc gtcaaattg 
      ttgcaggat actttagat tcgcttttt aacattttg 
      atagagttt accgaaacc gatacgtac gatatttac 
      gtttttgac tgtttgttg cacataatt agtctagtc 
      agtaacgac tggaagtac cagcacttc gagccggtc 
      ctggatttg tacattaag gagaatttc agtgcgact 
      ttggcccat aagaaattg atcagcgtc ctcaagaac 
      ataatcggg cgcgccaac agcaaaacg cccgatagc 
      aaagacgat ctcattttt aaaacaatg aagtcgctc 
      caatacgtg atgagattc gtctcgaaa tcgcgcatt 
      ttgatgaaa gtggtcgac gatgtcgag gacgatttc 
      gaggaaagt ctccgcgat ttgctgcaa gacatcacc 
      gacatgatg gccctcaca tcggacggg atcttgcgg 
      gagcaaggc gcctgcctt aagtacctc cccagcacg 
      attcccgac atcctgatg atttttgat gcgaaagag 
      ctgagcgtg attctgtgc aatatcatc acgaacatc 
      cctcccggc cgtctcacg aagcaaaaa atgatgaca 
      atcaacgaa atcgtccac agtaaactg tttctctac 
      acggaatgt cgcaggata atgctgccg atcttcacc 
      aagcaagtc aagaccctg ttcgagctg cacgaggag 
      gtcgagttg tgtataaaa atcctgagc gatattttg 
      gagctgctg ttccgtaag gacatcgga cccacgtat 
      gacgacatc aatgagatt ataaccacg gttttgcgg 
      actttaatt cacactcga gttggcatg cctaaggag 
      gatcctcaa ttggggaac ttggtcgcg ctaatgatc 
      gatattttc cggcaaatg tcggagcgc cactatgag 
      gcctacatc gagcgcttc aacacgcgt ttcgacgtt 
      ttagatttt ttgatggag attttgttg tttttccaa 
      aatttggtc gacgataag gtctttccg tacgactgg 
      tgcgacatg ataatgctc caaaacagc gttattttg 
      aaagctttg cggtttttc tctcatacg attcgtgac 
      cgtttcttc gaaagattc gaacatcaa gcctggaac 
      aacttcttt cactgtgct atcgctttc atgacccag 
      ccggctctc caactcgaa actttctcg ttcagtaaa 
      cggatgaaa atcatccga atctataaa gatatgcgg 
      cgcgagacc ggcttcgaa atcaggtca atgtggttc 
      aatttggga cagtataaa gtacagttc gttccgagt 
      ttggttggg gcgattcta gaaatgaca ctcataccg 
      gaaccggaa ttgcgtaaa gcaacgatt ccgattttc 
      ttcgatatg atgcagtgc gagttttac tcgtcgagg 
      ttcgaactc gagagctat ggcgatacg aaacgagat 
      tcgtcgcat atcaagggg aattttaac gatttcgag 
      aatgaaatg atcgcgaaa ttggatatt ctagtcgag 
      ggagggaaa ggagatgag aattacaaa gatttgttt 
      catgatatt atgatggag cattgcagc caacatgcc 
      acgatgaac gaaacgggg attaagttt gttaaaact 
      gtaacgcgg ttgatggaa cgcttgctc gaataccgg 
      tgtattatt accgatgag aacaaggaa aatcgcatg 
      agttgcact gtgagcctt ttggacttt tatgccgaa 
      atcaaccga aaagaaatg tacattcgc tacttgaat 
      aaactgtac gacctccat ttggagtgt gataattac 
      accgaagcc gcctacact atcgacttg cacgcgaaa 
      cttttaaat tggaccgac gaggattta ccccaactt 
      ttgaaaact aatcgacac cctaaagct cacacgcac 
      cgccaactc aaggaagct ctctattat aacattatc 
      gagaattac gacaggggc aaattatgg gagtgtgcg 
      atcaaaaag tgccaagaa ctggcctcg caattcgag 
      agcgaaacg tacgattac caccgactg agccagttg 
      cacaaaaga atgtcgacc ttttacgat gacattatg 
      aaaaaaggt cgcgcaaaa ccggagtac ttccgtgtc 
      gggtatttc ggcaagggc ttcccccaa tttctgcaa 
      aataaagtg tttgtatat cgggggaag gaatacgaa 
      cgacttacc gaattcaat acgagaata ttgaacgag 
      ttcccgaaa gcggaactt ttgaataaa ttgacgccg 
      ccgggggag gatatcacc gaatcggat aaacaatac 
      gtacaaata aataaagtt gatccggtc atggacgag 
      aaaaagcac cggttttcc ggcaaaccc gtctccgaa 
      caaattctc aattactac aaagtgaac gacatacag 
      aaatttacg ttttcgcgc ccgtttatc cgcaaggac 
      ccgtacatt gacgacaag aacgagttc ggtcacttg 
      tggctagag cggaccgaa ctgacaacg acgtaccct 
      ttgcccggg attttgcgc tggttccct gtgagctca 
      tcaacggtc catgagata tctcctctg cggaacgcc 
      atcgaaacg atggaaaaa gccaacaaa acgttgaag 
      aactacgtg gttttgttc aacaacgat aaggacatg 
      cagattaat ccgttgagc ttgactctg aacggcatc 
      ctggacgcc gccgtcatg ggaggcata aaaaattac 
      gaagaggcg ttctttacg gaaaaatac gagacgcat 
      catcaggaa gatgatgtg ctgttgcaa agactgaag 
      gatctgatc gctgatcag ataccgctg ctggagttg 
      tgcgtcaga atacacaag gaaaatgcc cctgaaaac 
      ttgcagccc ctgcagaag cggttcgag gactgtttt 
      gcgaaaatg caagagagt gtcgaggag aagtacgga 
      aaggggacc tgtgatatt aagttagat agtcaagta 
      acgatgagg aggcaccac tttgttctt cctgacccg 
      cgcttgtcc gacatgtcg atgctttca gatagcgtg 
      gatagtcgc agccgagtt tcgagtttg acgaaatcg 
      caagtcgct tcattaaaa cagtttact tctagtttt 
      aattttgca acgtcgccg agtatcagc agcaaatct 
      agcatcagt agcaacacc aaaagttta ttagtgtcc 
      ccatcgaaa agtctaaca acaactccg gccttaaat 
      cacaaaaag tcgttcaaa atcacccga gaaaaacgc 
      cgttcgagt aagtctgat cacacgccg agtccgtcc 
      ctacttccg gccactcag tggtacacc actgacgaa 
      aatacaaac ggatcacct atatttgaa ctcactgaa 
      gagttgacc ccaaaacgg cccttgcgc tccgaagtc 
      gagaaggag aaacgcttg agccggccc ccaagtggt 
      cagttttct tccaggtct agcagtatt tcagtgacg 
      ttaagagga accggaagt tcgagtagc agtaaccgg 
      gattcggtt ggtacgact gactcaagt gtgagtgag 
      gaagacgcg gtgccgccc cctttaccg ttaaaaaca 
      agagaagtc gattactgt aatttgccg aataacgaa 
      aacaccagt tttctttat tcacaacgt aattcagtc 
      aggactagt ttgaaaatt caggttcag gcggctgat 
      ccgataagt tttgatgac gtaccgccc acgccgccg 
      ccaaaaccg cccaaaaac aaaagcata tccaattta 
      tga
    mRNA sequence
    • >TC012454 RA
      ttttgatgg tgttttaaa gttttagta aaatgagtg 
      tgtgggaaa cagttcctg acgagctca gctacggca 
      tagcaatat acaatttcg ccgccgaca gcgacttca 
      aactagaac tgaccgtcg gggattccc tccatatac 
      tcgaccaag aagaaaact ggtactacg gctacctga 
      ttcacgaca gacacaaac gaggcattt tccccaaga 
      gttacgttc acattaaac catgcagtg ttgaccgca 
      ccggaccta ccctaacgt tcaatttca aagagccaa 
      caatcgtgc aagagataa cgtcggtgt tgcgagaat 
      gggccccgc attggaaaa acctctacg taaagaata 
      gcaaacatt tcgaagcca tcaaaaatc agatttacg 
      atctgataa cgcacagga gcaaaatca tcagcggta 
      ctttaccgg tcgacgaat tgaaacgga taacgaaac 
      aagtcactg aggagatcg ataagggga ataaaactt 
      tacgtttag atttaattg tgagaaaca aaaacggaa 
      atgtcatcg aaccggaaa agaccagca cgctgcaaa 
      tgtactatc tgcataaaa aagcgacgg aacggatga 
      gtaggacca ataagaacg aaaccaaac acaaccatc 
      ccaaaactg ccattcaac aatactcca atattttta 
      tagttgccg ttaaaaatt tcacctgta agatgaatg 
      aggatgccg agcttttaa tgtcgcttt acgacggaa 
      aagagtaca aatctatca cagaaaatt atgtcgtcc 
      ggtggtcta aagatggac tgatgtgcg acttggacc 
      agatgtaca atttgagag ttatgttta cggatctcg 
      ggaaacgcg atttagagc gggaaaaaa tttacttgg 
      tttgctacg tggtccgcg tgggtgcga tggaatcaa 
      aggagcccg accatcgcc ggagttccg tttcagtag 
      tgaacaaaa aaggcacaa ccgacggaa tccgtcgac 
      ctttcggtg tcgcagcga tggaaatca ctcatttca 
      tgaacgggt cgcgagaga gcgacttgg aacaagaat 
      ttcccgttc cttttttca actgcgata aggacaatc 
      tcgagcaaa ctttaaaga agataatta acaaagact 
      tcgccaaaa cggagcata aaaatcacg ctcttttcg 
      tcggaatga agttactgc ggggcgatt taaagcagg 
      tgcgggagg agaaccccc atctggtgc tgggaaatt 
      tgtcgatcg cccgaaaaa tgggcttcc cggaagtta 
      ttttaccgg gagacgtcc ggaacgatc tttatttga 
      ctttgatta aaggagagt tcagcaaag ggagcaaaa 
      cttgcgata aaaacgtcg aagttgtcg tcagagttt 
      gcaacgaag agggagtcc cgatccctg gggttatcg 
      cgttgggtt cgggggttc cgcccattg acgaataca 
      aatccatga tttattatc acgaggata aaccgcact 
      ggtatgaaa ctttcaaag tcgcgattc ctattgaag 
      aattcaaga cgagtcatt tgaaattca ctttcaaac 
      accggtctt ccaatgagg cgaaagaca aaaacgaga 
      agccgttcg ccatgtcct atgtgcgcc tcatgcagg 
      agaatggca cgactctca aagacgcgc gccataatt 
      tgattgtct acaaaatcg attacaaaa aattcgatg 
      aaaaaggcc tggattact tcaaattac cctcgattg 
      ttagcgacg tcaaggaca atgccaagc catcagtcg 
      ccggactga cgatgtcca aggacattt tcgtgatgt 
      cgagcaata tttgctcga cgaaactga cacagaatg 
      tggatttgt tgggtcttt tgaactgga aaacgcaca 
      aggatgact taaaatcgt gtcttcgag ccttgatca 
      aagtagacg gcgaagaag tcgtcaaat tgttgcagg 
      atactttag attcgcttt ttaacattt tgatagagt 
      ttaccgaaa ccgatacgt acgatattt acgtttttg 
      actgtttgt tgcacataa ttagtctag tcagtaacg 
      actggaagt accagcact tcgagccgg tcctggatt 
      tgtacatta aggagaatt tcagtgcga ctttggccc 
      ataagaaat tgatcagcg tcctcaaga acataatcg 
      ggcgcgcca acagcaaaa cgcccgata gcaaagacg 
      atctcattt ttaaaacaa tgaagtcgc tccaatacg 
      tgatgagat tcgtctcga aatcgcgca ttttgatga 
      aagtggtcg acgatgtcg aggacgatt tcgaggaaa 
      gtctccgcg atttgctgc aagacatca ccgacatga 
      tggccctca catcggacg ggatcttgc gggagcaag 
      gcgcctgcc ttaagtacc tccccagca cgattcccg 
      acatcctga tgatttttg atgcgaaag agctgagcg 
      tgattctgt gcaatatca tcacgaaca tccctcccg 
      gccgtctca cgaagcaaa aaatgatga caatcaacg 
      aaatcgtcc acagtaaac tgtttctct acacggaat 
      gtcgcagga taatgctgc cgatcttca ccaagcaag 
      tcaagaccc tgttcgagc tgcacgagg aggtcgagt 
      tgtgtataa aaatcctga gcgatattt tggagctgc 
      tgttccgta aggacatcg gacccacgt atgacgaca 
      tcaatgaga ttataacca cggttttgc ggactttaa 
      ttcacactc gagttggca tgcctaagg aggatcctc 
      aattgggga acttggtcg cgctaatga tcgatattt 
      tccggcaaa tgtcggagc gccactatg aggcctaca 
      tcgagcgct tcaacacgc gtttcgacg ttttagatt 
      ttttgatgg agattttgt tgtttttcc aaaatttgg 
      tcgacgata aggtctttc cgtacgact ggtgcgaca 
      tgataatgc tccaaaaca gcgttattt tgaaagctt 
      tgcggtttt tctctcata cgattcgtg accgtttct 
      tcgaaagat tcgaacatc aagcctgga acaacttct 
      ttcactgtg ctatcgctt tcatgaccc agccggctc 
      tccaactcg aaactttct cgttcagta aacggatga 
      aaatcatcc gaatctata aagatatgc ggcgcgaga 
      ccggcttcg aaatcaggt caatgtggt tcaatttgg 
      gacagtata aagtacagt tcgttccga gtttggttg 
      gggcgattc tagaaatga cactcatac cggaaccgg 
      aattgcgta aagcaacga ttccgattt tcttcgata 
      tgatgcagt gcgagtttt actcgtcga ggttcgaac 
      tcgagagct atggcgata cgaaacgag attcgtcgc 
      atatcaagg ggaatttta acgatttcg agaatgaaa 
      tgatcgcga aattggata ttctagtcg agggaggga 
      aaggagatg agaattaca aagatttgt ttcatgata 
      ttatgatgg agcattgca gccaacatg ccacgatga 
      acgaaacgg ggattaagt ttgttaaaa ctgtaacgc 
      ggttgatgg aacgcttgc tcgaatacc ggtgtatta 
      ttaccgatg agaacaagg aaaatcgca tgagttgca 
      ctgtgagcc ttttggact tttatgccg aaatcaacc 
      gaaaagaaa tgtacattc gctacttga ataaactgt 
      acgacctcc atttggagt gtgataatt acaccgaag 
      ccgcctaca ctatcgact tgcacgcga aacttttaa 
      attggaccg acgaggatt taccccaac ttttgaaaa 
      ctaatcgac accctaaag ctcacacgc accgccaac 
      tcaaggaag ctctctatt ataacatta tcgagaatt 
      acgacaggg gcaaattat gggagtgtg cgatcaaaa 
      agtgccaag aactggcct cgcaattcg agagcgaaa 
      cgtacgatt accaccgac tgagccagt tgcacaaaa 
      gaatgtcga ccttttacg atgacatta tgaaaaaag 
      gtcgcgcaa aaccggagt acttccgtg tcgggtatt 
      tcggcaagg gcttccccc aatttctgc aaaataaag 
      tgtttgtat atcggggga aggaatacg aacgactta 
      ccgaattca atacgagaa tattgaacg agttcccga 
      aagcggaac ttttgaata aattgacgc cgccggggg 
      aggatatca ccgaatcgg ataaacaat acgtacaaa 
      taaataaag ttgatccgg tcatggacg agaaaaagc 
      accggtttt ccggcaaac ccgtctccg aacaaattc 
      tcaattact acaaagtga acgacatac agaaattta 
      cgttttcgc gcccgttta tccgcaagg acccgtaca 
      ttgacgaca agaacgagt tcggtcact tgtggctag 
      agcggaccg aactgacaa cgacgtacc ctttgcccg 
      ggattttgc gctggttcc ctgtgagct catcaacgg 
      tccatgaga tatctcctc tgcggaacg ccatcgaaa 
      cgatggaaa aagccaaca aaacgttga agaactacg 
      tggttttgt tcaacaacg ataaggaca tgcagatta 
      atccgttga gcttgactc tgaacggca tcctggacg 
      ccgccgtca tgggaggca taaaaaatt acgaagagg 
      cgttcttta cggaaaaat acgagacgc atcatcagg 
      aagatgatg tgctgttgc aaagactga aggatctga 
      tcgctgatc agataccgc tgctggagt tgtgcgtca 
      gaatacaca aggaaaatg cccctgaaa acttgcagc 
      ccctgcaga agcggttcg aggactgtt ttgcgaaaa 
      tgcaagaga gtgtcgagg agaagtacg gaaagggga 
      cctgtgata ttaagttag atagtcaag taacgatga 
      ggaggcacc actttgttc ttcctgacc cgcgcttgt 
      ccgacatgt cgatgcttt cagacaaat ctagcatca 
      gtagcaaca ccaaaagtt tattagtgt ccccatcga 
      aaagtctaa caacaactc cggccttaa atcacaaaa 
      agtcgttca aaatcaccc gagaaaaac gccgttcga 
      gtaagtctg atcacacgc cgagtccgt ccctacttc 
      cggccactc agtggtaca ccactgacg aaaatacaa 
      acggatcac ctatatttg aactcactg aagagttga 
      ccccaaaac ggcccttgc gctccgaag tcgagaagg 
      agaaacgct tgagccggc ccccaagtg gtcagtttt 
      cttccaggt ctagcagta tttcagtga cgttaagag 
      gaaccggaa gttcgagta gcagtaacc gggattcgg 
      ttggtacga ctgactcaa gtgtgagtg aggaagacg 
      cggtgccgc cccctttac cgttaaaaa caagagaag 
      tcgattact gtaatttgc cgaataacg aaaacacca 
      gttttcttt attcacaac gtaattcag tcaggacta 
      gtttgaaaa ttcaggttc aggcggctg atccgataa 
      gttttgatg acgtaccgc ccacgccgc cgccaaaac 
      cgcccaaaa acaaaagca tatccaatt tatgaactg 
      taagcgtaa agtgaccaa gttaccaca agtgccatg 
      gatgtattt ttaactgta ttataacgc atatctagt 
      tttaatcat gtataaatt atttatatt attaaccaa 
      agatcgtat ttttttatt gtgaaggca ataaacttt 
      tttacgtat g
    • >TC012454 RB
      ttttgatgg tgttttaaa gttttagta aaatgagtg 
      tgtgggaaa cagttcctg acgagctca gctacggca 
      tagcaatat acaatttcg ccgccgaca gcgacttca 
      aactagaac tgaccgtcg gggattccc tccatatac 
      tcgaccaag aagaaaact ggtactacg gctacctga 
      ttcacgaca gacacaaac gaggcattt tccccaaga 
      gttacgttc acattaaac catgcagtg ttgaccgca 
      ccggaccta ccctaacgt tcaatttca aagagccaa 
      caatcgtgc aagagataa cgtcggtgt tgcgagaat 
      gggccccgc attggaaaa acctctacg taaagaata 
      gcaaacatt tcgaagcca tcaaaaatc agatttacg 
      atctgataa cgcacagga gcaaaatca tcagcggta 
      ctttaccgg tcgacgaat tgaaacgga taacgaaac 
      aagtcactg aggagatcg ataagggga ataaaactt 
      tacgtttag atttaattg tgagaaaca aaaacggaa 
      atgtcatcg aaccggaaa agaccagca cgctgcaaa 
      tgtactatc tgcataaaa aagcgacgg aacggatga 
      gtaggacca ataagaacg aaaccaaac acaaccatc 
      ccaaaactg ccattcaac aatactcca atattttta 
      tagttgccg ttaaaaatt tcacctgta agatgaatg 
      aggatgccg agcttttaa tgtcgcttt acgacggaa 
      aagagtaca aatctatca cagaaaatt atgtcgtcc 
      ggtggtcta aagatggac tgatgtgcg acttggacc 
      agatgtaca atttgagag ttatgttta cggatctcg 
      ggaaacgcg atttagagc gggaaaaaa tttacttgg 
      tttgctacg tggtccgcg tgggtgcga tggaatcaa 
      aggagcccg accatcgcc ggagttccg tttcagtag 
      tgaacaaaa aaggcacaa ccgacggaa tccgtcgac 
      ctttcggtg tcgcagcga tggaaatca ctcatttca 
      tgaacgggt cgcgagaga gcgacttgg aacaagaat 
      ttcccgttc cttttttca actgcgata aggacaatc 
      tcgagcaaa ctttaaaga agataatta acaaagact 
      tcgccaaaa cggagcata aaaatcacg ctcttttcg 
      tcggaatga agttactgc ggggcgatt taaagcagg 
      tgcgggagg agaaccccc atctggtgc tgggaaatt 
      tgtcgatcg cccgaaaaa tgggcttcc cggaagtta 
      ttttaccgg gagacgtcc ggaacgatc tttatttga 
      ctttgatta aaggagagt tcagcaaag ggagcaaaa 
      cttgcgata aaaacgtcg aagttgtcg tcagagttt 
      gcaacgaag agggagtcc cgatccctg gggttatcg 
      cgttgggtt cgggggttc cgcccattg acgaataca 
      aatccatga tttattatc acgaggata aaccgcact 
      ggtatgaaa ctttcaaag tcgcgattc ctattgaag 
      aattcaaga cgagtcatt tgaaattca ctttcaaac 
      accggtctt ccaatgagg cgaaagaca aaaacgaga 
      agccgttcg ccatgtcct atgtgcgcc tcatgcagg 
      agaatggca cgactctca aagacgcgc gccataatt 
      tgattgtct acaaaatcg attacaaaa aattcgatg 
      aaaaaggcc tggattact tcaaattac cctcgattg 
      ttagcgacg tcaaggaca atgccaagc catcagtcg 
      ccggactga cgatgtcca aggacattt tcgtgatgt 
      cgagcaata tttgctcga cgaaactga cacagaatg 
      tggatttgt tgggtcttt tgaactgga aaacgcaca 
      aggatgact taaaatcgt gtcttcgag ccttgatca 
      aagtagacg gcgaagaag tcgtcaaat tgttgcagg 
      atactttag attcgcttt ttaacattt tgatagagt 
      ttaccgaaa ccgatacgt acgatattt acgtttttg 
      actgtttgt tgcacataa ttagtctag tcagtaacg 
      actggaagt accagcact tcgagccgg tcctggatt 
      tgtacatta aggagaatt tcagtgcga ctttggccc 
      ataagaaat tgatcagcg tcctcaaga acataatcg 
      ggcgcgcca acagcaaaa cgcccgata gcaaagacg 
      atctcattt ttaaaacaa tgaagtcgc tccaatacg 
      tgatgagat tcgtctcga aatcgcgca ttttgatga 
      aagtggtcg acgatgtcg aggacgatt tcgaggaaa 
      gtctccgcg atttgctgc aagacatca ccgacatga 
      tggccctca catcggacg ggatcttgc gggagcaag 
      gcgcctgcc ttaagtacc tccccagca cgattcccg 
      acatcctga tgatttttg atgcgaaag agctgagcg 
      tgattctgt gcaatatca tcacgaaca tccctcccg 
      gccgtctca cgaagcaaa aaatgatga caatcaacg 
      aaatcgtcc acagtaaac tgtttctct acacggaat 
      gtcgcagga taatgctgc cgatcttca ccaagcaag 
      tcaagaccc tgttcgagc tgcacgagg aggtcgagt 
      tgtgtataa aaatcctga gcgatattt tggagctgc 
      tgttccgta aggacatcg gacccacgt atgacgaca 
      tcaatgaga ttataacca cggttttgc ggactttaa 
      ttcacactc gagttggca tgcctaagg aggatcctc 
      aattgggga acttggtcg cgctaatga tcgatattt 
      tccggcaaa tgtcggagc gccactatg aggcctaca 
      tcgagcgct tcaacacgc gtttcgacg ttttagatt 
      ttttgatgg agattttgt tgtttttcc aaaatttgg 
      tcgacgata aggtctttc cgtacgact ggtgcgaca 
      tgataatgc tccaaaaca gcgttattt tgaaagctt 
      tgcggtttt tctctcata cgattcgtg accgtttct 
      tcgaaagat tcgaacatc aagcctgga acaacttct 
      ttcactgtg ctatcgctt tcatgaccc agccggctc 
      tccaactcg aaactttct cgttcagta aacggatga 
      aaatcatcc gaatctata aagatatgc ggcgcgaga 
      ccggcttcg aaatcaggt caatgtggt tcaatttgg 
      gacagtata aagtacagt tcgttccga gtttggttg 
      gggcgattc tagaaatga cactcatac cggaaccgg 
      aattgcgta aagcaacga ttccgattt tcttcgata 
      tgatgcagt gcgagtttt actcgtcga ggttcgaac 
      tcgagagct atggcgata cgaaacgag attcgtcgc 
      atatcaagg ggaatttta acgatttcg agaatgaaa 
      tgatcgcga aattggata ttctagtcg agggaggga 
      aaggagatg agaattaca aagatttgt ttcatgata 
      ttatgatgg agcattgca gccaacatg ccacgatga 
      acgaaacgg ggattaagt ttgttaaaa ctgtaacgc 
      ggttgatgg aacgcttgc tcgaatacc ggtgtatta 
      ttaccgatg agaacaagg aaaatcgca tgagttgca 
      ctgtgagcc ttttggact tttatgccg aaatcaacc 
      gaaaagaaa tgtacattc gctacttga ataaactgt 
      acgacctcc atttggagt gtgataatt acaccgaag 
      ccgcctaca ctatcgact tgcacgcga aacttttaa 
      attggaccg acgaggatt taccccaac ttttgaaaa 
      ctaatcgac accctaaag ctcacacgc accgccaac 
      tcaaggaag ctctctatt ataacatta tcgagaatt 
      acgacaggg gcaaattat gggagtgtg cgatcaaaa 
      agtgccaag aactggcct cgcaattcg agagcgaaa 
      cgtacgatt accaccgac tgagccagt tgcacaaaa 
      gaatgtcga ccttttacg atgacatta tgaaaaaag 
      gtcgcgcaa aaccggagt acttccgtg tcgggtatt 
      tcggcaagg gcttccccc aatttctgc aaaataaag 
      tgtttgtat atcggggga aggaatacg aacgactta 
      ccgaattca atacgagaa tattgaacg agttcccga 
      aagcggaac ttttgaata aattgacgc cgccggggg 
      aggatatca ccgaatcgg ataaacaat acgtacaaa 
      taaataaag ttgatccgg tcatggacg agaaaaagc 
      accggtttt ccggcaaac ccgtctccg aacaaattc 
      tcaattact acaaagtga acgacatac agaaattta 
      cgttttcgc gcccgttta tccgcaagg acccgtaca 
      ttgacgaca agaacgagt tcggtcact tgtggctag 
      agcggaccg aactgacaa cgacgtacc ctttgcccg 
      ggattttgc gctggttcc ctgtgagct catcaacgg 
      tccatgaga tatctcctc tgcggaacg ccatcgaaa 
      cgatggaaa aagccaaca aaacgttga agaactacg 
      tggttttgt tcaacaacg ataaggaca tgcagatta 
      atccgttga gcttgactc tgaacggca tcctggacg 
      ccgccgtca tgggaggca taaaaaatt acgaagagg 
      cgttcttta cggaaaaat acgagacgc atcatcagg 
      aagatgatg tgctgttgc aaagactga aggatctga 
      tcgctgatc agataccgc tgctggagt tgtgcgtca 
      gaatacaca aggaaaatg cccctgaaa acttgcagc 
      ccctgcaga agcggttcg aggactgtt ttgcgaaaa 
      tgcaagaga gtgtcgagg agaagtacg gaaagggga 
      cctgtgata ttaagttag atagtcaag taacgatga 
      ggaggcacc actttgttc ttcctgacc cgcgcttgt 
      ccgacatgt cgatgcttt cagatagcg tggatagtc 
      gcagccgag tttcgagtt tgacgaaat cgcaagtcg 
      cttcattaa aacagttta cttctagtt ttaattttg 
      caacgtcgc cgagtatca gcagcaaat ctagcatca 
      gtagcaaca ccaaaagtt tattagtgt ccccatcga 
      aaagtctaa caacaactc cggccttaa atcacaaaa 
      agtcgttca aaatcaccc gagaaaaac gccgttcga 
      gtaagtctg atcacacgc cgagtccgt ccctacttc 
      cggccactc agtggtaca ccactgacg aaaatacaa 
      acggatcac ctatatttg aactcactg aagagttga 
      ccccaaaac ggcccttgc gctccgaag tcgagaagg 
      agaaacgct tgagccggc ccccaagtg gtcagtttt 
      cttccaggt ctagcagta tttcagtga cgttaagag 
      gaaccggaa gttcgagta gcagtaacc gggattcgg 
      ttggtacga ctgactcaa gtgtgagtg aggaagacg 
      cggtgccgc cccctttac cgttaaaaa caagagaag 
      tcgattact gtaatttgc cgaataacg aaaacacca 
      gttttcttt attcacaac gtaattcag tcaggacta 
      gtttgaaaa ttcaggttc aggcggctg atccgataa 
      gttttgatg acgtaccgc ccacgccgc cgccaaaac 
      cgcccaaaa acaaaagca tatccaatt tatgaactg 
      taagcgtaa agtgaccaa gttaccaca agtgccatg 
      gatgtattt ttaactgta ttataacgc atatctagt 
      tttaatcat gtataaatt atttatatt attaaccaa 
      agatcgtat ttttttatt gtgaaggca ataaacttt 
      tttacgtat g
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