Status
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Gene information
General information
TC011805 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13342652..13350813
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011805
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10626

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011805 RA
      MCPISGMVQ FLIVVVTLL LLCHAAVVR SLQVVFEPK 
      NATVHMHDT IPIAYQILA APDENLSPV RLYTENPNV 
      AKVDAFVIK NQSGTFNLT GNFLGKTEV RCQGAYGKL 
      DVTVIRKKR VIDTVFTSS VATLVSILY INFGCALNW 
      GEMIRNLKR PIGPAIGFV GQFVVMPLL SFGVGKLLF 
      PANPEMQLG MFFTGVSPA GGASNIWTV VLDGNMDLS 
      VTMTTISTL AAFGMMPMW LFTLGKVIF DQGDITVPY 
      SQISTYAIA LVIPLGIGF LLSRYCKKV ATFLVRILK 
      GFSAVLLLF IIVFAIVTN LYLFELFSW QIVLAGMGL 
      PYLGYLLGY LLAKILKQP APDCLAIAI ETGIQNTGI 
      AIFLLRFTL PQPQADLTT VAPVSVAMM TPLPLLIMF 
      VYKKIKARL YPARKKLNM AVQELTIDG NSNTQKEDR 
      LIPTIS
    CDS sequence
    • >TC011805 RA
      atgtgtccc atctcggga atggtccaa ttcctcatc 
      gtcgtcgtc accctcctc ctcctctgc cacgcggcc 
      gtcgtgcgc tccctgcag gtcgtcttc gagccgaag 
      aacgccacc gtccacatg cacgacacc atccccata 
      gcctaccag atcctcgca gcccccgac gagaacctc 
      agcccggtg cggctgtac accgagaac cccaacgtg 
      gccaaagtc gacgctttc gtcataaaa aatcagagc 
      gggacgttc aacttgact ggaaatttt ctaggtaag 
      acggaggtg aggtgtcag ggggcatat gggaagttg 
      gacgtgact gtaattcgc aaaaaacga gtcattgat 
      acggttttc acctcgtcg gtggccaca cttgtcagc 
      attttgtac atcaacttt ggctgtgcg cttaactgg 
      ggggaaatg atacggaat ttgaagcgg ccaattggg 
      cccgccatc ggcttcgtg ggacagttt gtcgtaatg 
      ccattgctc agttttggc gtgggaaag ctgctgttt 
      cccgccaac cccgagatg cagctcggt atgtttttc 
      acgggggtt tccccggcc ggcggtgcc tcaaacatc 
      tggacggtg gtccttgac ggcaacatg gacctgtcc 
      gtcactatg acaacaatc agtacctta gcagccttc 
      gggatgatg cccatgtgg ctcttcacc ctcggcaaa 
      gtgatcttc gatcagggc gacatcaca gtgccttat 
      tcccaaatc tccacctac gccatcgcg ctggtgatt 
      cccctaggc atcgggttt ttgctgtcg cgctattgc 
      aaaaaagtc gccacgttc ctggtccgc attttgaag 
      ggtttttct gccgtcctc ctcctgttc atcattgtg 
      ttcgcaatc gtgaccaac ttgtactta ttcgagttg 
      ttttcgtgg cagattgtc ctagcaggg atggggctg 
      ccctacctg gggtacctt ctggggtac ctccttgcc 
      aagatcctc aagcagccc gcccccgac tgtctcgca 
      attgcgata gaaacgggg attcaaaac acggggatc 
      gccattttt ctgctcagg ttcacactg ccgcagccc 
      caagccgac ctgaccaca gtggccccc gtctcagtg 
      gccatgatg acgccactg ccgctgcta attatgttc 
      gtttacaag aaaattaaa gccaggtta tatcccgct 
      cgcaagaag ctgaacatg gcggtgcaa gaattgaca 
      attgacgga aactcaaat acgcaaaag gaggatcgg 
      cttataccg accatttcg taa
    mRNA sequence
    • >TC011805 RA
      ggcgactct cgctgtctt cgtccaact cacggttac 
      aaaacagtc ccctctatt tatccttcg tttatcaat 
      tttttcagt ctcccattg gcactcgat tgtgatgtg 
      cacgtgttt tttattatt ttgcaatta tgtaaaata 
      acttgtgtt ttgtgattt gtgactcga aaaaggttt 
      ttgtagcta agatgtgtc ccatctcgg gaatggtcc 
      aattcctca tcgtcgtcg tcaccctcc tcctcctct 
      gccacgcgg ccgtcgtgc gctccctgc aggtcgtct 
      tcgagccga agaacgcca ccgtccaca tgcacgaca 
      ccatcccca tagcctacc agatcctcg cagcccccg 
      acgagaacc tcagcccgg tgcggctgt acaccgaga 
      accccaacg tggccaaag tcgacgctt tcgtcataa 
      aaaatcaga gcgggacgt tcaacttga ctggaaatt 
      ttctaggta agacggagg tgaggtgtc agggggcat 
      atgggaagt tggacgtga ctgtaattc gcaaaaaac 
      gagtcattg atacggttt tcacctcgt cggtggcca 
      cacttgtca gcattttgt acatcaact ttggctgtg 
      cgcttaact ggggggaaa tgatacgga atttgaagc 
      ggccaattg ggcccgcca tcggcttcg tgggacagt 
      ttgtcgtaa tgccattgc tcagttttg gcgtgggaa 
      agctgctgt ttcccgcca accccgaga tgcagctcg 
      gtatgtttt tcacggggg tttccccgg ccggcggtg 
      cctcaaaca tctggacgg tggtccttg acggcaaca 
      tggacctgt ccgtcacta tgacaacaa tcagtacct 
      tagcagcct tcgggatga tgcccatgt ggctcttca 
      ccctcggca aagtgatct tcgatcagg gcgacatca 
      cagtgcctt attcccaaa tctccacct acgccatcg 
      cgctggtga ttcccctag gcatcgggt ttttgctgt 
      cgcgctatt gcaaaaaag tcgccacgt tcctggtcc 
      gcattttga agggttttt ctgccgtcc tcctcctgt 
      tcatcattg tgttcgcaa tcgtgacca acttgtact 
      tattcgagt tgttttcgt ggcagattg tcctagcag 
      ggatggggc tgccctacc tggggtacc ttctggggt 
      acctccttg ccaagatcc tcaagcagc ccgcccccg 
      actgtctcg caattgcga tagaaacgg ggattcaaa 
      acacgggga tcgccattt ttctgctca ggttcacac 
      tgccgcagc cccaagccg acctgacca cagtggccc 
      ccgtctcag tggccatga tgacgccac tgccgctgc 
      taattatgt tcgtttaca agaaaatta aagccaggt 
      tatatcccg ctcgcaaga agctgaaca tggcggtgc 
      aagaattga caattgacg gaaactcaa atacgcaaa 
      aggaggatc ggcttatac cgaccattt cgtaatctc 
      tgtgattgg ttaaaacgc gacttaaat taccaaaaa 
      ttcggctaa tgtgcaaaa gtgggactc accgcaatt 
      atttcctcg ccaactcac tcgtgaagt aaatgagta 
      cccgtttct gataaaaac attaaaatc atcggagct 
      tggagattt tttgctcaa aactgcgtg tttaggtcc 
      aagattaac acaaatttt gtgtttttt tggctaatt 
      ttcaaccat attttccca aaaaaacga ggtaaatcg 
      agtgaaacg ggcagaata accccaaat gcgatttat 
      ttttggggc cggttttat aagaaagct ccattaaaa 
      tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY