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Gene information
General information
TC014776 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14922612..14935365
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014776
    iBeetle screen iB_10622

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva potentially partiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva potentiallyeverted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014776 RA
      MDAGYTLTL ENPKRTANI ISKLFFLWM VKLCYKGTK 
      KGLEIADLY KTLSCDQSE KLTDELEKH WNEEVEKNK 
      LKLQKPPSL TRAIVKTFL WKYMGFGIL LFVQNIVFR 
      AFQPVILAY FINLFSGEG QDNQNEMYI FGSVLVIQT 
      FFIVITMHH IDLGQASIG MRIRVAVSS LIYRKMLKL 
      NKRSLGSAS AGQVVNLLS NDVNRFDFI TLALHYLWI 
      MPFQVVLVT YLIWREMGV STLAGVLSM LCLTLPVQG 
      YLGKLTSKL RLKTAQRTD YRVKLMNEI ISGIQIIKM 
      YAWEKPFEQ IVKQARKHE IDVVTQASY LRGIYLSCM 
      VFIERTTLF LTITCYVLL GNPITADKV FSIAQFYNI 
      LQLALAICY PMAITFGAE TLVSIKRLC DFLVLEEKP 
      QSQIERKAE QDIEFDNTS GAWNSDSLT LQNLDLFIP 
      QGTLCAIVG PVGAGKSSI LQMLLGELP PITGSIKVG 
      GKISYASQE PWLFAATVR NNILFGREY DRALYREVV 
      KVCALERDF KQFPQGDRT VVGERGVSL SGGQRARIN 
      LARAVYRGG DVYLLDDPL SAVDTHVGR HLFDECIVK 
      YLRGKTRVL ITHQLQYLK KADHIVVLN EGRIEAQGK 
      FQELINSDL DFTKLLASQ DETEKEETA KAPRKSSVV 
      SHKSNVSES SEFFEPSDD MEDLDYSNS SPFKDYIKA 
      SGNKCAVFG LLLVLLLGQ SACSAADYW VTFWTQQEA 
      YRYLNSTQI IQKSENYSQ LTDDILIDN QEVYLIKTE 
      VAMYIYGGI IAFAIFFTL VRSFAFFKM AMTASKNLH 
      GKMFHALLQ APMRFFDTN PSGRVLNRF SKDMGAIDE 
      FLPRVLVEA IQILLVMSG ILVMVTIAN YYMVVAMVI 
      IGLLFLKVR SWYVATAKD VKHLEGITK SNVYSHLNS 
      SFSGITTIR AAEAEVMLA KEFDKHQDN HTSAWFLTI 
      ATRVCFGLW LDLLSIVFI FCVIFSFIV LNQFTQVSG 
      SLVGLAISQ SLILTGMLQ FGMRQTAEV VNQLTSVER 
      VMQYTKLDS EFTETKKTV SFPWPSKGM IEFQNLSLK 
      YSEFDPPVL RHLNLTIAP GAKIGIVGR TGAGKSSLI 
      SALFRLAPI EGKILIDGI DTKTIDLNR LRKKISIIP 
      QAPVLFSAT LRYNLDPFQ EFDDTKLWD VLEQVELKE 
      SIRHLDVPV SEGGSNFSL GQRQLLCLA RAILRNNQI 
      LVLDEATAN VDPRWTDAL IQQTIRQKF HNCTVLTIA 
      HRLNTIMDS DRVLVMDSG KVAEFDHPH LLLQDEDGH 
      FAKMVAETG PAMTQQLKQ IAHDCTISK LFFLWIIPL 
      YRKIIKNGL QICDLCKIL ESDESEKVS DKLENNWNK 
      ELLRAKLKN GQPSLLKAI GATFFWKYM SFGAVLFIQ 
      HVFLRSFQP IVLSYLISL FGQTDPNHT AMYVSSGIL 
      VTLSLLIVL SMHQVNFGH ASIGMRIRI AISALVYRK 
      TLRLNRRSL NQTSIGQIV NLLSNDVTR FDLVVLTLH 
      YLWILPFQV SIITFLCWS QVGISSLVG VVSIALLSL 
      PVQGYLGKL TSNYRVKVA QKTDHRVTL MNEIVSGIQ 
      VIKMYGWEK PFEHIVRLA RSQEVKALT ITSYLRGIY 
      LSAMIFVER TALFLTLSC YVFNGNTIL AQHVFSISQ 
      FFNLLQLTM SIFYPLSIS YGAEALVSI DRIQAFLQM 
      EEVEPSKIE TDFNHGVTL SNVNSQLLK NITFKIPQG 
      TLCAIVGPV GSGKTSLLH LLLNESSSK CGKITLQGS 
      ISYAAQEPW LFASTIRKN ILFGNKYDR HTYNKVVKV 
      CALKKDFDQ FPLSDKTLV GERGSALSG GQRARVNLA 
      RAVYKDSDI YLLDDPLSA VDAHVGNHL FEQCILKYL 
      KGKTRILVT HQLQFLKRV DHIIVLKNG QIEAQGTYA 
      ELSHSKLDF PTGKRDEEV AKPDSDLHT LSDSFMLES 
      TNYKNEVED IESTGMSEG ATSLIEYVM ASGTLCQIF 
      LVSLALLVC QTLCSGTDF WVTFWTQQE ALRNITINE 
      TLTVPVTQT IDVFPHNDS LTDSYSYTY NDEKQIVKE 
      VTVSKALIE TRTALYVYL ALIVVLIIV TFLRSILYF 
      TLAMKASRN LHNNMFTTL LQAQMKFFN SNPSGRILN 
      RFSKDMGAI DEILPKVLL EAIQITLTM CGILVMVII 
      SNQYMIPVV ILLGVVFSK IRSWFVTTT KNIKHLEGI 
      TKSPVFSHM NSSLYGITT IRACGAEEM LKKEFDRHQ 
      DVHTSSWFL LITTTSSFG LWLDLVCVA FIGFTSFSF 
      ILLNHYYQI SGSLVGLAI SQSLILTGM LQYGVRQSA 
      EVVNQLTSV ERILQYSEI EKEGPFNTS PEHRPPPFW 
      PDKGQIELR DMSLHYSPA KPPVLKNIT VKIAPGQKI 
      GIVGRTGAG KSSLIAALF RLSDISGTI YIDGVDTKK 
      LGVHDLRKK ISIIPQVPV LFSSTVRYN LDPFGDFED 
      GKLWDVLDE VELKDSVVS LDAEVARDG GNFSVGQRQ 
      LICLARAIL KNNKILVMD EATANTDDK TDALIQKMI 
      RKRFKSCTV ITVAHRLHT VMDSDRIIV MDDGRVVEF 
      DHPYNLLQR PDTTFYKMV LETGLETSV YLEDMALDA 
      FLNKQSIPS AKEQ
    CDS sequence
    • >TC014776 RA
      atggacgcc ggttacacc ctaactctc gaaaacccc 
      aaacggacg gcaaacatc atctccaaa ctgtttttc 
      ctatggatg gtaaaactg tgctacaaa gggaccaaa 
      aaaggtctc gaaatcgcc gacttgtac aaaactcta 
      tcatgtgat caatctgaa aaactcacc gatgagttg 
      gagaaacac tggaatgaa gaagtcgag aaaaataaa 
      ttaaaacta caaaaaccg ccaagtctg acacgcgca 
      atcgtcaag acttttctc tggaaatac atggggttc 
      gggatttta ttatttgtg caaaatatc gtgttccgg 
      gcgttccag ccggtcatt ctggcctat tttattaat 
      ttattttcg ggcgaaggc caggataat cagaacgaa 
      atgtacatt tttgggtcg gtcttagta attcagacc 
      ttttttatc gtcataaca atgcaccac atcgaccta 
      ggacaagcc tcaatcggg atgcggatc cgagtggca 
      gtctcgtca ctcatttac cgaaaaatg ttgaagctc 
      aataagcgg tctttaggc tcagcatca gctggccaa 
      gtggtcaat ttgctctca aacgatgtc aatcgtttc 
      gatttcatc actttggcc cttcactat ttatggata 
      atgcccttc caagtcgtt ctagtgact tatttgatc 
      tggcgggag atgggagtc tcgacctta gctggagtt 
      ctctccatg ctatgtctc accctccca gttcagggg 
      tatttgggc aaactgacc tcaaaactg cgcctcaaa 
      acggcccaa aggaccgac taccgtgtc aaactcatg 
      aacgaaata atctccggc atccaaatc atcaaaatg 
      tacgcttgg gaaaaaccc ttcgaacaa atcgtaaaa 
      caggcccgt aaacacgaa attgacgtc gtcacccaa 
      gcctcatac ttgcgagga atttacttg agttgtatg 
      gtgtttata gagcgcacc actttattc ctgacaatc 
      acatgttac gtccttttg ggcaaccca ataaccgcg 
      gataaagtc ttctcaatt gcccagttt tacaacatt 
      ttgcaacta gctttagcc atttgttac cccatggct 
      ataactttc ggggctgaa acgctggtc tcgatcaaa 
      cgactttgc gacttcctg gttttggag gaaaaaccg 
      caatcgcaa atcgagcga aaggcggag caagacatc 
      gagttcgat aacaccagt ggtgcctgg aatagcgac 
      tctttgact ctccaaaat ttggattta tttatcccg 
      caagggacg ctctgtgct atcgtgggc cctgtgggg 
      gccggcaaa tcctcaatc ctccaaatg ttgcttgga 
      gagctgcct ccaatcact gggtcgatc aaagtggga 
      ggaaaaatc tcgtatgcg tcacaggaa ccgtggctt 
      tttgcagca actgtccga aataatatt ctgttcgga 
      cgggagtat gacagagcc ttgtatcgt gaagtggta 
      aaggtttgt gccttggag cgggatttt aagcagttt 
      ccgcaaggc gataggaca gtagttggg gaacgagga 
      gtgtctttg agtgggggc caaagggca aggattaac 
      ctagctagg gctgtgtat agaggaggg gatgtttac 
      ctccttgat gatcctctc tcggctgtt gatacacac 
      gtagggagg caccttttt gacgagtgc attgtcaag 
      tatttgagg ggcaagacg agggttttg atcacgcac 
      cagttgcaa tatttgaag aaagccgac cacattgtg 
      gtcctcaat gaaggacgg attgaagca caaggaaag 
      ttccaagaa ctgatcaat agtgattta gactttacg 
      aaattatta gcctctcag gacgagaca gaaaaggaa 
      gaaaccgcc aaggcgcca agaaaatcg tccgttgtc 
      tcgcataaa agtaacgta agtgagtca agcgagttt 
      tttgagcca agtgacgat atggaagac ctggactat 
      tcgaattcc tcgccgttt aaagactat atcaaagct 
      tcggggaac aaatgtgca gtttttggc ctgcttttg 
      gtgcttttg ctgggacag tcggcgtgc agtgctgcc 
      gattattgg gtcactttt tggacccaa caggaggcc 
      tacagatac ctaaactca acccaaatc atccaaaaa 
      tcggaaaat tattcccaa ctcactgac gacatcctc 
      atcgacaat caagaggtg tatttgatc aaaaccgaa 
      gttgcgatg tacatttat gggggcatt atcgctttc 
      gcgatcttt ttcacactt gtgcgatcc ttcgcattc 
      ttcaaaatg gcaatgact gcgtccaaa aacctacat 
      ggtaaaatg ttccacgcc cttttgcaa gccccaatg 
      agatttttc gacacgaac cccagtggg cgcgtcttg 
      aaccgattt tcgaaagac atgggggcc attgacgag 
      tttcttccg cgggttttg gtggaagcc atccaaatt 
      cttttggtg atgagcggg atcttggtc atggtcacg 
      atcgccaac tattacatg gtcgtagcg atggttata 
      atcgggcta ttgttccta aaagtgagg tcctggtac 
      gtggccacg gccaaagac gtcaaacac ttggaaggg 
      atcacaaaa tcaaacgtc tattcgcac ttgaactcg 
      tcattcagt ggcattacg acaattcgg gccgctgaa 
      gccgaggtc atgttggca aaggagttt gacaaacac 
      caggataat cacacatcg gcctggttt ttgaccatt 
      gcgaccaga gtttgcttc ggcctatgg ctggacttg 
      ctgagcatc gttttcatt ttttgtgtc atttttagt 
      ttcattgtt ctgaaccaa ttcactcaa gtgagtgga 
      agtttggtc ggtttggcc atttcccag tcactcatc 
      ctcaccgga atgttacaa ttcggcatg agacaaacc 
      gccgaagtc gtcaatcag ctcacaagt gtggaacgc 
      gtcatgcaa tacacaaaa cttgacagc gaattcacg 
      gaaaccaaa aaaacagtc tcttttccc tggcctagt 
      aaaggcatg attgaattc cagaacctt tccttgaaa 
      tactccgag tttgatcct ccggttctc cgccacttg 
      aacctaacc atagccccc ggagccaaa atcgggatt 
      gttgggcgg accggagcc ggaaaatcg tccctgatt 
      tcggcgctt ttccgcctt gcgccaatc gaggggaaa 
      attttgatc gacggtatt gacacaaaa accatcgac 
      ttgaaccgc ttgaggaaa aaaatctcg attattccc 
      caagcacct gtgcttttt tcggcaacg ctgcgctat 
      aatttggac ccgtttcaa gagtttgat gatacaaaa 
      ttgtgggat gttttggaa caggttgaa ttaaaagag 
      agcattcgg catttggac gttcccgtg tctgagggc 
      gggagcaat tttagtcta ggacagagg cagctcttg 
      tgtttggcg agggccatc ctgaggaat aatcaaatt 
      ctggttttg gatgaggca acagccaat gtggatcca 
      aggtggact gatgcttta atccaacaa accatccgc 
      caaaaattc cacaattgc acggtcttg accattgcc 
      catcgttta aacaccata atggactcg gatagggtt 
      ctggtaatg gattcgggg aaagtagcg gagtttgac 
      catccccac ttattactg caagatgaa gatgggcat 
      tttgccaaa atggtcgca gaaacaggg cctgcgatg 
      acgcagcaa ttaaagcaa attgcacat gattgcaca 
      atatcaaaa ctgtttttt ctttggatt attcctttg 
      tatcgtaaa ataattaaa aatggatta caaatatgt 
      gacctgtgt aaaattctg gaatcggac gaatcggaa 
      aaagtgagc gacaagctg gaaaacaac tggaacaaa 
      gagctgctc agagctaag ttaaaaaac ggacaaccc 
      agtttgctc aaagcaatc ggggccact tttttctgg 
      aaatatatg agttttggc gcggttctc ttcatacaa 
      catgttttc cttaggtct tttcagcca attgttctg 
      tcctatttg atatcactt ttcgggcaa actgacccc 
      aaccacacc gccatgtac gtctcctct ggaattctg 
      gtaacactc tcactcctt atagtcttg tccatgcac 
      caggtgaat ttcggacac gcctcaatc gggatgcgg 
      atccgaatc gcaatttcc gcactcgtt taccgaaaa 
      actttaaga ctgaaccgg aggtcactc aaccagacc 
      tcaatcggc caaatcgtc aacctcctc tcaaacgat 
      gtcacgcgg ttcgacctg gtggtcctg actttgcac 
      tatctctgg attttgccg ttccaagtg tcgattatc 
      acatttttg tgttggtcc caagtcggc atttcgtcg 
      ctggttgga gtggtttca atcgcactt ttgtcactc 
      cctgtccaa ggctatttg ggcaagtta acatcgaac 
      tatcgcgtc aaagtggcg caaaaaacc gaccaccgg 
      gtgacttta atgaacgaa atagtgtcg ggaatccag 
      gtcattaaa atgtacggg tgggaaaag ccctttgaa 
      catattgtc cggctggcg agaagccag gaggtcaaa 
      gcattgact attacttcg tacttgcgg gggatttac 
      ttaagtgcg atgattttt gtcgaacga accgccctg 
      tttttgact ttgtcttgc tacgtattt aacggtaac 
      acaatcctg gcccaacac gtgttctca atatcacag 
      ttcttcaat ttattgcaa ctgaccatg tcgatattt 
      tacccactt tccataagt tacggagct gaggcactt 
      gtgtcaatt gaccggatc caagccttc ctccagatg 
      gaagaagtg gagccttcg aaaatcgag actgacttt 
      aaccacggt gttactcta agcaatgtc aattcacaa 
      ttgctgaaa aacatcacg ttcaaaatc ccgcaagga 
      acactctgt gccattgtg ggccctgtg gggtcgggt 
      aagacgtcc ttactgcac ctcctcctc aacgaatcg 
      tcctccaag tgcggcaaa atcaccctc caagggtcg 
      atttcttac gccgcgcaa gaaccttgg ctttttgcc 
      tcaacaatc cgaaaaaac atcctgttc ggtaacaaa 
      tacgacaga cacacgtac aacaaagtg gtcaaagtg 
      tgcgctttg aaaaaagac ttcgaccag tttcccctc 
      tctgataag acccttgtt ggggaaagg ggctcagct 
      ttgagtggg gggcaaagg gctagggtt aacctagcc 
      cgggctgtg tacaaagac tccgacatt tacctcctg 
      gatgaccct ttatccgca gttgacgca cacgttggt 
      aaccactta ttcgaacaa tgcatcctg aaatacttg 
      aaaggcaaa accaggatt ttggtcacc caccagtta 
      caattcttg aaacgcgtt gaccacatt attgtgctc 
      aaaaacggg caaattgag gcacaaggg acttacgcg 
      gagctgagt cactcaaaa ttggacttt ccgacgggg 
      aaaagggac gaagaggtg gcgaaacca gacagcgat 
      ttgcacact ttgagtgat agttttatg cttgaatcc 
      acaaattac aagaacgaa gtcgaggat attgaaagt 
      acagggatg tctgaaggg gcgacctca ttgattgag 
      tacgtgatg gcctcaggg accctctgc caaattttt 
      ctggtgtct ttggctttg ctcgtgtgc cagactttg 
      tgcagtggg accgatttt tgggtcacg ttttggacc 
      caacaggag gctctccga aatatcacg attaatgaa 
      actttgacg gttcctgtg acccaaact attgatgtt 
      ttcccccac aatgatagt ttaaccgac tcttattcc 
      tacacatac aatgacgag aaacaaata gttaaagaa 
      gtaacagtt agcaaagct ttgatcgaa acccgaact 
      gcgctttac gtttatttg gctctgatc gtagttctg 
      attatcgtt acgttcctc cgatcaatt ttatacttc 
      actctggcg atgaaagcg tccagaaac ctccacaac 
      aacatgttc accaccctg ctccaggcc caaatgaaa 
      ttttttaat agcaacccc agtgggcgc attttgaac 
      cgattttcc aaagacatg ggagctatt gatgaaatt 
      ctaccgaaa gttttactc gaagcgatt caaatcact 
      ttgaccatg tgtggtatt ttggtcatg gtgatcatc 
      tcaaatcag tacatgatt ccagttgtg attctcctc 
      ggagttgtt ttttccaaa atacggtcg tggtttgtc 
      accacgacg aaaaatatc aaacatttg gaaggaata 
      actaaatcc ccggttttt tcgcatatg aattcaagc 
      ttgtacggg attacgacg ataagggcc tgcggggct 
      gaggagatg ctcaaaaag gagtttgat cgacatcag 
      gacgtacat acttcctcc tggtttttg ctaattaca 
      acaacatcg tcattcgga ttatggcta gatttagta 
      tgtgtggca tttataggc tttacttcc ttcagtttt 
      attctactg aaccattat taccagatt agtggaagt 
      ctcgtcggt ctggccatc tctcaatcg cttattctg 
      accggaatg ctgcaatac ggagttcgt caaagtgca 
      gaagtggtc aaccaactc acttccgtc gaacgaatt 
      ctccagtat tccgaaatc gagaaagaa ggacctttc 
      aacacttct cctgaacac cgaccaccg cctttctgg 
      cctgacaag ggccaaatc gagctgcgc gacatgtcc 
      ctgcactac tccccagcc aagcccccg gtgttgaaa 
      aacataacg gtgaaaatc gccccaggt caaaaaatc 
      gggattgtg ggccggaca ggtgccggg aaatcgtcc 
      ctaattgcg gcacttttc cgcctgagt gacatttcc 
      gggacgatt tacattgac ggagtggat acgaagaag 
      ttgggggtg cacgatttg agaaaaaaa atctcgatt 
      atcccacaa gtgccagtg ctgttttcg tcaactgtg 
      cggtacaat ttggatcca tttggggat tttgaagat 
      ggaaaactt tgggatgtt ttggacgaa gtggagttg 
      aaagattcg gttgtgagt ctggacgct gaggttgcc 
      cgtgatggg gggaatttt agtgtgggg caaagacaa 
      ctgatttgc ctggcgagg gcgattttg aaaaataat 
      aaaatactg gtgatggac gaggctacg gccaatacg 
      gacgataag acggatgct ctcattcaa aaaatgatc 
      cggaagaga tttaagtca tgcactgtg attacagtg 
      gctcataga ctccatacc gttatggac tcagatcgg 
      attattgtg atggacgat ggccgagta gtcgaattc 
      gaccaccct tataatctc ttacaaagg cctgacaca 
      actttttat aaaatggtt ttagagacg ggactagag 
      accagcgtt tacttggaa gatatggcc ctcgatgca 
      tttctcaat aagcagtca attccaagc gccaaggaa 
      caataa
    mRNA sequence
    • >TC014776 RA
      agtgtcagg tgtaggatg gacgccggt tacacccta 
      actctcgaa aaccccaaa cggacggca aacatcatc 
      tccaaactg tttttccta tggatggta aaactgtgc 
      tacaaaggg accaaaaaa ggtctcgaa atcgccgac 
      ttgtacaaa actctatca tgtgatcaa tctgaaaaa 
      ctcaccgat gagttggag aaacactgg aatgaagaa 
      gtcgagaaa aataaatta aaactacaa aaaccgcca 
      agtctgaca cgcgcaatc gtcaagact tttctctgg 
      aaatacatg gggttcggg attttatta tttgtgcaa 
      aatatcgtg ttccgggcg ttccagccg gtcattctg 
      gcctatttt attaattta ttttcgggc gaaggccag 
      gataatcag aacgaaatg tacattttt gggtcggtc 
      ttagtaatt cagaccttt tttatcgtc ataacaatg 
      caccacatc gacctagga caagcctca atcgggatg 
      cggatccga gtggcagtc tcgtcactc atttaccga 
      aaaatgttg aagctcaat aagcggtct ttaggctca 
      gcatcagct ggccaagtg gtcaatttg ctctcaaac 
      gatgtcaat cgtttcgat ttcatcact ttggccctt 
      cactattta tggataatg cccttccaa gtcgttcta 
      gtgacttat ttgatctgg cgggagatg ggagtctcg 
      accttagct ggagttctc tccatgcta tgtctcacc 
      ctcccagtt caggggtat ttgggcaaa ctgacctca 
      aaactgcgc ctcaaaacg gcccaaagg accgactac 
      cgtgtcaaa ctcatgaac gaaataatc tccggcatc 
      caaatcatc aaaatgtac gcttgggaa aaacccttc 
      gaacaaatc gtaaaacag gcccgtaaa cacgaaatt 
      gacgtcgtc acccaagcc tcatacttg cgaggaatt 
      tacttgagt tgtatggtg tttatagag cgcaccact 
      ttattcctg acaatcaca tgttacgtc cttttgggc 
      aacccaata accgcggat aaagtcttc tcaattgcc 
      cagttttac aacattttg caactagct ttagccatt 
      tgttacccc atggctata actttcggg gctgaaacg 
      ctggtctcg atcaaacga ctttgcgac ttcctggtt 
      ttggaggaa aaaccgcaa tcgcaaatc gagcgaaag 
      gcggagcaa gacatcgag ttcgataac accagtggt 
      gcctggaat agcgactct ttgactctc caaaatttg 
      gatttattt atcccgcaa gggacgctc tgtgctatc 
      gtgggccct gtgggggcc ggcaaatcc tcaatcctc 
      caaatgttg cttggagag ctgcctcca atcactggg 
      tcgatcaaa gtgggagga aaaatctcg tatgcgtca 
      caggaaccg tggcttttt gcagcaact gtccgaaat 
      aatattctg ttcggacgg gagtatgac agagccttg 
      tatcgtgaa gtggtaaag gtttgtgcc ttggagcgg 
      gattttaag cagtttccg caaggcgat aggacagta 
      gttggggaa cgaggagtg tctttgagt gggggccaa 
      agggcaagg attaaccta gctagggct gtgtataga 
      ggaggggat gtttacctc cttgatgat cctctctcg 
      gctgttgat acacacgta gggaggcac ctttttgac 
      gagtgcatt gtcaagtat ttgaggggc aagacgagg 
      gttttgatc acgcaccag ttgcaatat ttgaagaaa 
      gccgaccac attgtggtc ctcaatgaa ggacggatt 
      gaagcacaa ggaaagttc caagaactg atcaatagt 
      gatttagac tttacgaaa ttattagcc tctcaggac 
      gagacagaa aaggaagaa accgccaag gcgccaaga 
      aaatcgtcc gttgtctcg cataaaagt aacgtaagt 
      gagtcaagc gagtttttt gagccaagt gacgatatg 
      gaagacctg gactattcg aattcctcg ccgtttaaa 
      gactatatc aaagcttcg gggaacaaa tgtgcagtt 
      tttggcctg cttttggtg cttttgctg ggacagtcg 
      gcgtgcagt gctgccgat tattgggtc actttttgg 
      acccaacag gaggcctac agataccta aactcaacc 
      caaatcatc caaaaatcg gaaaattat tcccaactc 
      actgacgac atcctcatc gacaatcaa gaggtgtat 
      ttgatcaaa accgaagtt gcgatgtac atttatggg 
      ggcattatc gctttcgcg atctttttc acacttgtg 
      cgatccttc gcattcttc aaaatggca atgactgcg 
      tccaaaaac ctacatggt aaaatgttc cacgccctt 
      ttgcaagcc ccaatgaga tttttcgac acgaacccc 
      agtgggcgc gtcttgaac cgattttcg aaagacatg 
      ggggccatt gacgagttt cttccgcgg gttttggtg 
      gaagccatc caaattctt ttggtgatg agcgggatc 
      ttggtcatg gtcacgatc gccaactat tacatggtc 
      gtagcgatg gttataatc gggctattg ttcctaaaa 
      gtgaggtcc tggtacgtg gccacggcc aaagacgtc 
      aaacacttg gaagggatc acaaaatca aacgtctat 
      tcgcacttg aactcgtca ttcagtggc attacgaca 
      attcgggcc gctgaagcc gaggtcatg ttggcaaag 
      gagtttgac aaacaccag gataatcac acatcggcc 
      tggtttttg accattgcg accagagtt tgcttcggc 
      ctatggctg gacttgctg agcatcgtt ttcattttt 
      tgtgtcatt tttagtttc attgttctg aaccaattc 
      actcaagtg agtggaagt ttggtcggt ttggccatt 
      tcccagtca ctcatcctc accggaatg ttacaattc 
      ggcatgaga caaaccgcc gaagtcgtc aatcagctc 
      acaagtgtg gaacgcgtc atgcaatac acaaaactt 
      gacagcgaa ttcacggaa accaaaaaa acagtctct 
      tttccctgg cctagtaaa ggcatgatt gaattccag 
      aacctttcc ttgaaatac tccgagttt gatcctccg 
      gttctccgc cacttgaac ctaaccata gcccccgga 
      gccaaaatc gggattgtt gggcggacc ggagccgga 
      aaatcgtcc ctgatttcg gcgcttttc cgccttgcg 
      ccaatcgag gggaaaatt ttgatcgac ggtattgac 
      acaaaaacc atcgacttg aaccgcttg aggaaaaaa 
      atctcgatt attccccaa gcacctgtg cttttttcg 
      gcaacgctg cgctataat ttggacccg tttcaagag 
      tttgatgat acaaaattg tgggatgtt ttggaacag 
      gttgaatta aaagagagc attcggcat ttggacgtt 
      cccgtgtct gagggcggg agcaatttt agtctagga 
      cagaggcag ctcttgtgt ttggcgagg gccatcctg 
      aggaataat caaattctg gttttggat gaggcaaca 
      gccaatgtg gatccaagg tggactgat gctttaatc 
      caacaaacc atccgccaa aaattccac aattgcacg 
      gtcttgacc attgcccat cgtttaaac accataatg 
      gactcggat agggttctg gtaatggat tcggggaaa 
      gtagcggag tttgaccat ccccactta ttactgcaa 
      gatgaagat gggcatttt gccaaaatg gtcgcagaa 
      acagggcct gcgatgacg cagcaatta aagcaaatt 
      gcacatgat tgcacaata tcaaaactg ttttttctt 
      tggattatt cctttgtat cgtaaaata attaaaaat 
      ggattacaa atatgtgac ctgtgtaaa attctggaa 
      tcggacgaa tcggaaaaa gtgagcgac aagctggaa 
      aacaactgg aacaaagag ctgctcaga gctaagtta 
      aaaaacgga caacccagt ttgctcaaa gcaatcggg 
      gccactttt ttctggaaa tatatgagt tttggcgcg 
      gttctcttc atacaacat gttttcctt aggtctttt 
      cagccaatt gttctgtcc tatttgata tcacttttc 
      gggcaaact gaccccaac cacaccgcc atgtacgtc 
      tcctctgga attctggta acactctca ctccttata 
      gtcttgtcc atgcaccag gtgaatttc ggacacgcc 
      tcaatcggg atgcggatc cgaatcgca atttccgca 
      ctcgtttac cgaaaaact ttaagactg aaccggagg 
      tcactcaac cagacctca atcggccaa atcgtcaac 
      ctcctctca aacgatgtc acgcggttc gacctggtg 
      gtcctgact ttgcactat ctctggatt ttgccgttc 
      caagtgtcg attatcaca tttttgtgt tggtcccaa 
      gtcggcatt tcgtcgctg gttggagtg gtttcaatc 
      gcacttttg tcactccct gtccaaggc tatttgggc 
      aagttaaca tcgaactat cgcgtcaaa gtggcgcaa 
      aaaaccgac caccgggtg actttaatg aacgaaata 
      gtgtcggga atccaggtc attaaaatg tacgggtgg 
      gaaaagccc tttgaacat attgtccgg ctggcgaga 
      agccaggag gtcaaagca ttgactatt acttcgtac 
      ttgcggggg atttactta agtgcgatg atttttgtc 
      gaacgaacc gccctgttt ttgactttg tcttgctac 
      gtatttaac ggtaacaca atcctggcc caacacgtg 
      ttctcaata tcacagttc ttcaattta ttgcaactg 
      accatgtcg atattttac ccactttcc ataagttac 
      ggagctgag gcacttgtg tcaattgac cggatccaa 
      gccttcctc cagatggaa gaagtggag ccttcgaaa 
      atcgagact gactttaac cacggtgtt actctaagc 
      aatgtcaat tcacaattg ctgaaaaac atcacgttc 
      aaaatcccg caaggaaca ctctgtgcc attgtgggc 
      cctgtgggg tcgggtaag acgtcctta ctgcacctc 
      ctcctcaac gaatcgtcc tccaagtgc ggcaaaatc 
      accctccaa gggtcgatt tcttacgcc gcgcaagaa 
      ccttggctt tttgcctca acaatccga aaaaacatc 
      ctgttcggt aacaaatac gacagacac acgtacaac 
      aaagtggtc aaagtgtgc gctttgaaa aaagacttc 
      gaccagttt cccctctct gataagacc cttgttggg 
      gaaaggggc tcagctttg agtgggggg caaagggct 
      agggttaac ctagcccgg gctgtgtac aaagactcc 
      gacatttac ctcctggat gacccttta tccgcagtt 
      gacgcacac gttggtaac cacttattc gaacaatgc 
      atcctgaaa tacttgaaa ggcaaaacc aggattttg 
      gtcacccac cagttacaa ttcttgaaa cgcgttgac 
      cacattatt gtgctcaaa aacgggcaa attgaggca 
      caagggact tacgcggag ctgagtcac tcaaaattg 
      gactttccg acggggaaa agggacgaa gaggtggcg 
      aaaccagac agcgatttg cacactttg agtgatagt 
      tttatgctt gaatccaca aattacaag aacgaagtc 
      gaggatatt gaaagtaca gggatgtct gaaggggcg 
      acctcattg attgagtac gtgatggcc tcagggacc 
      ctctgccaa atttttctg gtgtctttg gctttgctc 
      gtgtgccag actttgtgc agtgggacc gatttttgg 
      gtcacgttt tggacccaa caggaggct ctccgaaat 
      atcacgatt aatgaaact ttgacggtt cctgtgacc 
      caaactatt gatgttttc ccccacaat gatagttta 
      accgactct tattcctac acatacaat gacgagaaa 
      caaatagtt aaagaagta acagttagc aaagctttg 
      atcgaaacc cgaactgcg ctttacgtt tatttggct 
      ctgatcgta gttctgatt atcgttacg ttcctccga 
      tcaatttta tacttcact ctggcgatg aaagcgtcc 
      agaaacctc cacaacaac atgttcacc accctgctc 
      caggcccaa atgaaattt tttaatagc aaccccagt 
      gggcgcatt ttgaaccga ttttccaaa gacatggga 
      gctattgat gaaattcta ccgaaagtt ttactcgaa 
      gcgattcaa atcactttg accatgtgt ggtattttg 
      gtcatggtg atcatctca aatcagtac atgattcca 
      gttgtgatt ctcctcgga gttgttttt tccaaaata 
      cggtcgtgg tttgtcacc acgacgaaa aatatcaaa 
      catttggaa ggaataact aaatccccg gttttttcg 
      catatgaat tcaagcttg tacgggatt acgacgata 
      agggcctgc ggggctgag gagatgctc aaaaaggag 
      tttgatcga catcaggac gtacatact tcctcctgg 
      tttttgcta attacaaca acatcgtca ttcggatta 
      tggctagat ttagtatgt gtggcattt ataggcttt 
      acttccttc agttttatt ctactgaac cattattac 
      cagattagt ggaagtctc gtcggtctg gccatctct 
      caatcgctt attctgacc ggaatgctg caatacgga 
      gttcgtcaa agtgcagaa gtggtcaac caactcact 
      tccgtcgaa cgaattctc cagtattcc gaaatcgag 
      aaagaagga cctttcaac acttctcct gaacaccga 
      ccaccgcct ttctggcct gacaagggc caaatcgag 
      ctgcgcgac atgtccctg cactactcc ccagccaag 
      cccccggtg ttgaaaaac ataacggtg aaaatcgcc 
      ccaggtcaa aaaatcggg attgtgggc cggacaggt 
      gccgggaaa tcgtcccta attgcggca cttttccgc 
      ctgagtgac atttccggg acgatttac attgacgga 
      gtggatacg aagaagttg ggggtgcac gatttgaga 
      aaaaaaatc tcgattatc ccacaagtg ccagtgctg 
      ttttcgtca actgtgcgg tacaatttg gatccattt 
      ggggatttt gaagatgga aaactttgg gatgttttg 
      gacgaagtg gagttgaaa gattcggtt gtgagtctg 
      gacgctgag gttgcccgt gatgggggg aattttagt 
      gtggggcaa agacaactg atttgcctg gcgagggcg 
      attttgaaa aataataaa atactggtg atggacgag 
      gctacggcc aatacggac gataagacg gatgctctc 
      attcaaaaa atgatccgg aagagattt aagtcatgc 
      actgtgatt acagtggct catagactc cataccgtt 
      atggactca gatcggatt attgtgatg gacgatggc 
      cgagtagtc gaattcgac cacccttat aatctctta 
      caaaggcct gacacaact ttttataaa atggtttta 
      gagacggga ctagagacc agcgtttac ttggaagat 
      atggccctc gatgcattt ctcaataag cagtcaatt 
      ccaagcgcc aaggaacaa taatgtgta agataggca 
      aatataaaa atatattcc ttcctcgta g
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