Status
Please wait ...
Gene information
General information
TC032997
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016313
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 971032..981643
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032997
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10619

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Fertility
      mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
      mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
      ovary without detectable defects - Number of animals showing the phenotype: 2
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head structure irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      head position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      head structure split - Penetrance (percentage of animals/eggs on slide): less than 30%

      labrum sometimes potentiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      legs potentially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      prothorax mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      posterior terminus structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032997 RA
        MFLHIVLVI AAVCSSQGM PQRETPQIT SRIGESKQL 
        LYLPERQQY TPELQYDLS LLDPQFQVP SYNFIGDKQ 
        HFPRVQPQI VLINGGATP GQFLLQPGS PPGNFLVPQ 
        GPNVILRDP QGPQRPVFV AGYPKPAHI PPIEKDAEE 
        VPTNPAKIP PLTPKGEKK PEKLETFDE PDNSKKQAT 
        EAEVLAAAD RNPAFNTRP NLRPGQRFF ILNGQPLFS 
        NFPYNNQIY PGINPNLKY AQVAPSFVP QTPTKNISP 
        FQNVLFRNT PVENTHKPT GSDFHQSYP ENLVLLNQP 
        GIFQDDVIF RSPALPLNL VQKPKDEPQ LRENENGYG 
        LFHLRSKEE QEDDTIVVD AKEQQDGSV DDNNDEQDT 
        EAEVVSAAD KPDSEPTIS QAQPGAIAL AGPGGVAAA 
        APRGTAFVG KEGVAVSSP QATAVAGPS KDAIQEKNK 
        KQNKRKQ
      CDS sequence
      • >TC032997 RA
        atgtttttg cacattgtc ttggtcata gcagcggtt 
        tgctcctca caaggcatg cctcagaga gaaactcct 
        caaatcaca tcccggatt ggagaatcc aagcaactt 
        ctttatctt cctgaaaga caacaatac acccctgaa 
        ttgcagtat gacctatct ttgctagat ccacaattt 
        caagtgcct tcatacaat ttcatcgga gataaacaa 
        catttcccg agagtgcaa ccacaaatc gtcttgatc 
        aacggtggc gccactcca ggtcaattc ctcctccag 
        cctggatcc ccccctggc aatttcctg gtgccccaa 
        ggccctaac gtgatcctt cgggatccc caaggacct 
        caaagacct gtcttcgtt gcaggatac cccaaaccg 
        gctcatatt ccaccaatt gagaaagat gccgaagaa 
        gtgccaacc aatccagca aaaattcca ccacttaca 
        cccaaagga gaaaagaag cctgaaaaa ttggaaacg 
        ttcgacgaa cccgacaac tcgaaaaaa caagcaact 
        gaagctgaa gttcttgcc gcagctgac cgaaaccca 
        gccttcaac accaggcca aatttgcgt ccaggccaa 
        agatttttc atcctgaat ggccaacct ctcttctcc 
        aatttccct tacaacaac caaatttac ccaggaatt 
        aaccccaac ctgaaatac gctcaagta gctcccagt 
        ttcgttccc caaaccccc accaaaaac atctccccc 
        ttccaaaac gtcctcttc agaaacact ccggttgaa 
        aacacacac aaaccaact ggcagcgat tttcaccaa 
        tcttaccct gaaaatcta gtcctactc aaccaaccc 
        ggaattttc caggacgat gttattttc cggagccct 
        gctcttccc ttgaatctt gtacagaag ccaaaagac 
        gagccccag ctcagagaa aacgaaaat ggttatggt 
        ctgttccat ttgaggagc aaagaggag caagaggat 
        gatacgatt gtagtagat gctaaagag caacaagac 
        ggaagtgtt gatgataat aacgatgag caagacaca 
        gaggctgag gttgtgtca gctgctgat aagccagac 
        agcgagcca acgatttcg caagcccaa ccgggggcc 
        atagccctt gccggccct gggggcgtc gctgcagca 
        gcccccagg ggaactgct ttcgtcgga aaggaagga 
        gttgccgtt tcgagtcct caagctact gctgttgcg 
        ggaccaagc aaggacgcc atccaggag aagaataaa 
        aagcaaaat aagagaaag cagtga
      mRNA sequence
      • >TC032997 RA
        gaggagtta ttgcttatc tatactctt gtgcaccaa 
        ggtgttgga acacttagt cgacaagat gtttttgca 
        cattgtctt ggtcatagc agcggtttg ctcctcaca 
        aggcatgcc tcagagaga aactcctca aatcacatc 
        ccggattgg agaatccaa gcaacttct ttatcttcc 
        tgaaagaca acaatacac ccctgaatt gcagtatga 
        cctatcttt gctagatcc acaatttca agtgccttc 
        atacaattt catcggaga taaacaaca tttcccgag 
        agtgcaacc acaaatcgt cttgatcaa cggtggcgc 
        cactccagg tcaattcct cctccagcc tggatcccc 
        ccctggcaa tttcctggt gccccaagg ccctaacgt 
        gatccttcg ggatcccca aggacctca aagacctgt 
        cttcgttgc aggataccc caaaccggc tcatattcc 
        accaattga gaaagatgc cgaagaagt gccaaccaa 
        tccagcaaa aattccacc acttacacc caaaggaga 
        aaagaagcc tgaaaaatt ggaaacgtt cgacgaacc 
        cgacaactc gaaaaaaca agcaactga agctgaagt 
        tcttgccgc agctgaccg aaacccagc cttcaacac 
        caggccaaa tttgcgtcc aggccaaag atttttcat 
        cctgaatgg ccaacctct cttctccaa tttccctta 
        caacaacca aatttaccc aggaattaa ccccaacct 
        gaaatacgc tcaagtagc tcccagttt cgttcccca 
        aacccccac caaaaacat ctccccctt ccaaaacgt 
        cctcttcag aaacactcc ggttgaaaa cacacacaa 
        accaactgg cagcgattt tcaccaatc ttaccctga 
        aaatctagt cctactcaa ccaacccgg aattttcca 
        ggacgatgt tattttccg gagccctgc tcttccctt 
        gaatcttgt acagaagcc aaaagacga gccccagct 
        cagagaaaa cgaaaatgg ttatggtct gttccattt 
        gaggagcaa agaggagca agaggatga tacgattgt 
        agtagatgc taaagagca acaagacgg aagtgttga 
        tgataataa cgatgagca agacacaga ggctgaggt 
        tgtgtcagc tgctgataa gccagacag cgagccaac 
        gatttcgca agcccaacc gggggccat agcccttgc 
        cggccctgg gggcgtcgc tgcagcagc ccccagggg 
        aactgcttt cgtcggaaa ggaaggagt tgccgtttc 
        gagtcctca agctactgc tgttgcggg accaagcaa 
        ggacgccat ccaggagaa gaataaaaa gcaaaataa 
        gagaaagca gtgattttt gtgtgatat ttagatgat 
        ttcgaatcg ctgaattca aataaaatt gatggtttt 
        atttttaaa ctatttata agcatttgg aaaacattg 
        cgaatgtgg taatttttg taactatta ccatctcga 
        atgcttctc taattaatt agaaatgta atgaaacac 
        ataataaaa tatactttt taat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY