Status
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Gene information
General information
TC013715 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5455804..5461988
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013715
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10618

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013715 RA
      MLILVDVIS VLVVIFAIF AYFVYTACR SFYGMDPDE 
      AKYMRTERK PGTLDVHPT LNAIVLNYE IDVQILGAK 
      ENVIYGEKK NLKKVIELP MLNSRTDCY ALAKEVVYQ 
      CDLIHHSRI SEVEQTIYY LKKRKLSHG SAKDNNSSH 
      DLKQIINAD EPNYNNLSD YIELLYEGM SEKIKGAHF 
      IQLLARDPE NLDALSKNE TVISALARV LREDWKRSI 
      VLSTHLVFT FFCFSMYSR FHEVILKCK VGSICMDII 
      DYELRRYDK WKADLEGVE APDDIPIVR KPCPSSASL 
      SEIPRSRIP EPVRPRSGN FSEANIKAV MEGSVYDDL 
      TTSTESIDD KKLSEAEKA KRFRTLVKK QEHLLRVAF 
      YLLLNIAED ESIEEKMTK RNIVGLLVK ALERDNEEL 
      LVLVVTFLK KLSIMQCNK DAMAGMNVV EKLPKLLES 
      NSPDLVHLT LKLLFNLSF DTKLRFRIV QIGLLPKFI 
      SLLSDDRHQ EIVLKLLYH LSYDDDVKT QFAECVGLI 
      TDMLLLNIG NEGDEVMVA LCINLATDP TNAQQMMKK 
      NRVQSLMMR AFNYQDNML MKMLRNLSE HKSSKVNFV 
      EFVGDIAKA VVESKEDDF VVECVGILS NLSLPELDW 
      AEIFKHFDM LKWIEKVIR NNNTDPELV LQVVVLLGT 
      AAADEGCAK LLCESNIIS ALIELLKTH QEDDEIVLQ 
      IIFVFFATI SHGTNIDYL AEKTEAPAY LIDLLQDNN 
      KSIRKLCNT CLNIIAEHS KTWAERIKI EKFRHHNAQ 
      WLQMVDSQQ LGPEDEDDE EDALPPYLN TEFLGTAVV 
      PPLSDLNEH AQESEFISQ KDIDFDYFD RPEVIPDFE 
      IEAI
    CDS sequence
    • >TC013715 RA
      atgttaatt cttgtcgat gtgatatcg gttctggtg 
      gttattttt gcgatattt gcgtatttc gtgtacacg 
      gcctgtcgg agtttctac ggaatggac ccggacgag 
      gccaagtac atgcggacg gagagaaaa cccggcact 
      ttggacgtg cacccgact ctgaacgct atcgttttg 
      aattacgaa attgatgtc cagattttg ggggcgaaa 
      gagaacgtc atctacggc gagaaaaag aacctgaaa 
      aaagtgata gaactccca atgctgaac agcagaact 
      gattgttac gccttagcc aaagaagtt gtctaccag 
      tgtgacttg atccatcat tcgcgcatc tcggaggtc 
      gagcaaacc atttactac ttaaagaag cgaaaattg 
      agccacgga agtgctaaa gacaataac agttcccat 
      gatctcaaa caaataatc aacgccgac gaacccaat 
      tacaacaat ttgtcggat tacatcgag ctgctctac 
      gagggaatg tctgaaaaa atcaagggc gctcacttc 
      atccagctt ttggcgcgt gacccggaa aatttggac 
      gcattatcg aaaaatgaa actgtaata agcgcctta 
      gcgcgagtt ctacgcgag gattggaaa cgaagtatt 
      gtcttaagc acacacctg gtgtttacc tttttctgt 
      ttttccatg tactcacgt tttcacgag gttatactc 
      aagtgtaag gtgggttcg atctgtatg gacataatc 
      gattacgag ttgcgaaga tacgacaag tggaaggcg 
      gatttggag ggcgttgag gccccggac gacatcccc 
      atcgtccgc aagccatgt ccgagtagc gctagcctc 
      tccgaaatc ccccggagt cgcattccg gagccggtt 
      aggccccga tccgggaac ttctccgag gcgaatatt 
      aaagccgtg atggaaggc agcgtttac gacgatttg 
      acgacttcc acggaaagt atcgacgat aaaaaactg 
      agcgaagcg gaaaaggcg aaacgtttc cgcactttg 
      gtgaaaaaa caagaacat ctgctccgt gtcgcgttt 
      tatctcttg ctgaatatc gccgaagac gaaagtatt 
      gaagagaaa atgacgaag aggaacatc gtggggttg 
      ttggtcaag gctctggag agggacaac gaggaattg 
      ttggttctg gtggtgacg ttcctgaag aagttgtcc 
      attatgcag tgtaataag gacgctatg gccgggatg 
      aatgtcgtg gagaagttg ccgaaattg ctggagtcc 
      aacagtccc gacttggtg catttgacg ttgaaattg 
      ttgtttaat ttgtcgttt gatacaaaa ttgaggttt 
      agaatcgtc caaattgga cttctaccg aaatttatc 
      agtttatta agcgatgat cgtcatcag gagattgtt 
      ttaaaatta ctctaccat ttgagttac gacgatgac 
      gtcaaaacg caatttgcc gaatgtgtg ggtttgata 
      acggacatg ttattactt aatattgga aacgaaggt 
      gacgaagtt atggtcgca ctttgtatc aatttggcc 
      acggacccg acaaacgcc caacaaatg atgaaaaaa 
      aatcgcgtc caatcgtta atgatgagg gctttcaat 
      tatcaagat aacatgttg atgaaaatg ttgaggaat 
      ttgtcagag cataaatca tccaaagtt aatttcgtc 
      gagtttgta ggcgacatt gcaaaggcc gtagtcgag 
      tcaaaggaa gacgatttc gtagtcgaa tgtgtcgga 
      attttaagc aacctcagt ttacccgag ctggactgg 
      gcggaaatt ttcaaacac ttcgatatg ttaaaatgg 
      atcgaaaaa gtgattcgt aacaataac accgacccc 
      gagttggtt ttacaagtt gtagtttta ttaggcacg 
      gctgcagcc gacgaaggt tgtgccaag ctcttgtgc 
      gaatcgaac ataatctcg gctttgatc gaactcttg 
      aagacgcac caggaagac gacgaaatc gtccttcaa 
      ataattttc gtttttttc gcgaccatt tctcacggg 
      acgaatatt gattatctg gcggagaaa accgaagct 
      ccggcctat ttgatcgat ttactccaa gacaataac 
      aagtccatt aggaaattg tgcaatacg tgtcttaat 
      ataatagcc gagcatagt aaaacttgg gcggaaagg 
      atcaaaatt gaaaaattc aggcatcat aacgcgcag 
      tggttacaa atggtcgat tcgcagcag ttgggtccc 
      gaggacgag gacgacgaa gaggatgcc ttgccaccg 
      tatctgaat acggagttt ctgggaacg gcagtggtc 
      ccgcctttg tcagatttg aatgaacat gcacaagaa 
      tcggaattt atctcacaa aaagacata gactttgac 
      tattttgat agaccagag gtcattccg gactttgaa 
      attgaagcc atttaa
    mRNA sequence
    • >TC013715 RA
      tcggcaaag acgggtgcg cgcggtatc aaccaactc 
      aaatgtaat gtcaacaat aaaatgcac aaactttgc 
      ttaattgtt taatgttaa ttcttgtcg atgtgatat 
      cggttctgg tggttattt ttgcgatat ttgcgtatt 
      tcgtgtaca cggcctgtc ggagtttct acggaatgg 
      acccggacg aggccaagt acatgcgga cggagagaa 
      aacccggca ctttggacg tgcacccga ctctgaacg 
      ctatcgttt tgaattacg aaattgatg tccagattt 
      tgggggcga aagagaacg tcatctacg gcgagaaaa 
      agaacctga aaaaagtga tagaactcc caatgctga 
      acagcagaa ctgattgtt acgccttag ccaaagaag 
      ttgtctacc agtgtgact tgatccatc attcgcgca 
      tctcggagg tcgagcaaa ccatttact acttaaaga 
      agcgaaaat tgagccacg gaagtgcta aagacaata 
      acagttccc atgatctca aacaaataa tcaacgccg 
      acgaaccca attacaaca atttgtcgg attacatcg 
      agctgctct acgagggaa tgtctgaaa aaatcaagg 
      gcgctcact tcatccagc ttttggcgc gtgacccgg 
      aaaatttgg acgcattat cgaaaaatg aaactgtaa 
      taagcgcct tagcgcgag ttctacgcg aggattgga 
      aacgaagta ttgtcttaa gcacacacc tggtgttta 
      cctttttct gtttttcca tgtactcac gttttcacg 
      aggttatac tcaagtgta aggtgggtt cgatctgta 
      tggacataa tcgattacg agttgcgaa gatacgaca 
      agtggaagg cggatttgg agggcgttg aggccccgg 
      acgacatcc ccatcgtcc gcaagccat gtccgagta 
      gcgctagcc tctccgaaa tcccccgga gtcgcattc 
      cggagccgg ttaggcccc gatccggga acttctccg 
      aggcgaata ttaaagccg tgatggaag gcagcgttt 
      acgacgatt tgacgactt ccacggaaa gtatcgacg 
      ataaaaaac tgagcgaag cggaaaagg cgaaacgtt 
      tccgcactt tggtgaaaa aacaagaac atctgctcc 
      gtgtcgcgt tttatctct tgctgaata tcgccgaag 
      acgaaagta ttgaagaga aaatgacga agaggaaca 
      tcgtggggt tgttggtca aggctctgg agagggaca 
      acgaggaat tgttggttc tggtggtga cgttcctga 
      agaagttgt ccattatgc agtgtaata aggacgcta 
      tggccggga tgaatgtcg tggagaagt tgccgaaat 
      tgctggagt ccaacagtc ccgacttgg tgcatttga 
      cgttgaaat tgttgttta atttgtcgt ttgatacaa 
      aattgaggt ttagaatcg tccaaattg gacttctac 
      cgaaattta tcagtttat taagcgatg atcgtcatc 
      aggagattg ttttaaaat tactctacc atttgagtt 
      acgacgatg acgtcaaaa cgcaatttg ccgaatgtg 
      tgggtttga taacggaca tgttattac ttaatattg 
      gaaacgaag gtgacgaag ttatggtcg cactttgta 
      tcaatttgg ccacggacc cgacaaacg cccaacaaa 
      tgatgaaaa aaaatcgcg tccaatcgt taatgatga 
      gggctttca attatcaag ataacatgt tgatgaaaa 
      tgttgagga atttgtcag agcataaat catccaaag 
      ttaatttcg tcgagtttg taggcgaca ttgcaaagg 
      ccgtagtcg agtcaaagg aagacgatt tcgtagtcg 
      aatgtgtcg gaattttaa gcaacctca gtttacccg 
      agctggact gggcggaaa ttttcaaac acttcgata 
      tgttaaaat ggatcgaaa aagtgattc gtaacaata 
      acaccgacc ccgagttgg ttttacaag ttgtagttt 
      tattaggca cggctgcag ccgacgaag gttgtgcca 
      agctcttgt gcgaatcga acataatct cggctttga 
      tcgaactct tgaagacgc accaggaag acgacgaaa 
      tcgtccttc aaataattt tcgtttttt tcgcgacca 
      tttctcacg ggacgaata ttgattatc tggcggaga 
      aaaccgaag ctccggcct atttgatcg atttactcc 
      aagacaata acaagtcca ttaggaaat tgtgcaata 
      cgtgtctta atataatag ccgagcata gtaaaactt 
      gggcggaaa ggatcaaaa ttgaaaaat tcaggcatc 
      ataacgcgc agtggttac aaatggtcg attcgcagc 
      agttgggtc ccgaggacg aggacgacg aagaggatg 
      ccttgccac cgtatctga atacggagt ttctgggaa 
      cggcagtgg tcccgcctt tgtcagatt tgaatgaac 
      atgcacaag aatcggaat ttatctcac aaaaagaca 
      tagactttg actattttg atagaccag aggtcattc 
      cggactttg aaattgaag ccatttaat tttttgctt 
      gagcacaag tgatattca gctttctag tcatacatt 
      ttcttgtta atttttatg taattgata ataaacatt 
      tacttgtac t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY