Status
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Gene information
General information
TC008488 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 11430645..11445461
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008488
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10617

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008488 RA
      MAGPDVAAL AADLARAVE LTMSTGASQ TDRLKAYNA 
      CESFKETSP LCAEAGLYL AAGTQHSLI SRHFGLQLM 
      EHTVKYRWT QISQQEKIF IKENAMKLL AAGGISDEP 
      HMKDALSRV IVEMVKREW PQQWPGLLS ELSEACSCG 
      EIQTELVLL VFLRLVEDV ALLQTLESN QRRKDIYHA 
      LTANMAVIF DFFLRLIEL HVNQFRICG ETNNTPKST 
      AHGRVVQVV LLTLTGFVE WVSMSHIMA QNGRLLHIL 
      CLLLNDLAF QYPAAECLS QIVNRKGKV DERKPLLLL 
      FNDEPIQCL VSASKNPGA ILDEQHYLF KKKLVQVLG 
      GLTTQLVVL WGKDSISRP NNFSAFLEA ILAFSSHQS 
      LTLSHMANP LWNSMLKHE HISRDPVFL SYIPQWVQC 
      TAPKIVKFN YPASKVQNT DTGGAAAYA KIDFDSEEE 
      FSTYFYRCR SDFLDSFRQ ATVVAPLVT FNYVEQWLM 
      KCLQVPNVT SGLVLSDPL FHEWEALST FLESILSRV 
      LQAQERPSI ASGLRLLQL CLVYQPVDP LILSTLLTC 
      ISALFVFLS MSTGQMAPT ANSVAASGA ALLPQVLDK 
      IFSTLVYAP PDEQSKDTR SRAVKNVRR HAASLMVKI 
      GNKYPLLLL PVFDQIRAT VENLSRSDS VAGLSTLEK 
      VTLQEALLL ISNHFCDYD RQSNFVREV LAEANAQWR 
      LIVASGAFE SASKFISFV GLDTPPVAP HADNPHGHN 
      RSSIVFCIN LLLGAIKRC SWPEDPERA TRGGFVVAL 
      TESGNPVCR NPAAPHVVP LLPDILSLI RVFNELFTC 
      EAQNLIHES YKGCLGMLE TEKSNLLGL IGHSVGDLG 
      ELQAVQSPM ERMQRFLFG LHESCYHMI GSMGPSLGR 
      DLYTLPDIG LAIINSVLA CLQCIPDYR MRPIIRVFL 
      KPFIYSCPT PFYEAVLLP IVAHIAPLM LSRLHAKWL 
      QVNEFRNRE GQEDNADTQ EVLEDILTR ALTREYLDV 
      LKVALVGGG LTPETNTEN METEDLSMD SPTPPPPTR 
      SNMTTEVIS DLGLVLLRS EKTCQSIVL AVLGALSWI 
      DSNASLKAT FLTGPIVRQ LVSDSSLNG EMAAHIMAS 
      VLNALMLHG QHEANQGSL LTLGAQMYE MLRPTFLEV 
      LGVMQQIPG VNPVDLQKL DERISGSTS KGNKVEKVK 
      KDLFRKITG NLIGRSMGQ LFKKEVKIH DLPSLAFSK 
      KPQPKEVTP DLQNVFS
    CDS sequence
    • >TC008488 RA
      atggctgga ccagacgtt gcggctctt gctgcggat 
      ttagcaaga gccgttgaa ctcacaatg agcaccgga 
      gcttcgcaa accgaccga ctcaaagca tacaacgcg 
      tgcgaaagc tttaaagag acttcacct ctgtgtgcc 
      gaagctggg ctctatttg gccgcaggg acgcaacat 
      tccttgatt tcgagacat ttcggcttg cagttgatg 
      gagcatacg gtcaagtat cgctggacg caaatttcc 
      caacaggag aagatcttc atcaaggaa aatgcaatg 
      aagttgctt gctgctgga gggattagt gacgaaccg 
      cacatgaag gatgcttta agtcgagtt attgtggaa 
      atggtgaag agagaatgg ccacaacaa tggccaggt 
      ttgctctca gagctgtcg gaagcttgt tcttgtggg 
      gagattcaa actgaattg gttttgttg gtgtttcta 
      cgattagtt gaggatgtt gctttactg cagacgctg 
      gaatcaaat cagcgaaga aaagacatc tatcatgcc 
      ctcaccgcc aatatggct gtcatcttt gattttttt 
      cttcgtcta atcgaatta cacgtgaac caatttcgt 
      atatgtggt gaaacaaat aacacaccg aaatctacc 
      gctcatggt agagtcgtt caagttgtt ttactgact 
      ttgactggt tttgttgaa tgggtctct atgtcacac 
      attatggct caaaatgga agactccta cacattctg 
      tgtctgtta ttgaacgat ttggcgttt cagtatccg 
      gcggctgaa tgtttatca cagattgta aatagaaaa 
      ggtaaagtt gacgaacga aaaccactc ttgctcctc 
      ttcaacgac gaaccaatc caatgttta gtttcggcg 
      tcaaaaaat cccggtgcc attctcgac gaacaacac 
      tatttattt aagaagaaa cttgtacag gttttaggc 
      ggtttaaca actcaactt gttgtctta tggggtaaa 
      gattctatt tcacgtccc aacaacttt tcggctttt 
      ttggaagca attcttgca tttagtagc catcaaagt 
      ctcacatta tcacatatg gccaaccct ctgtggaac 
      agtatgctc aaacacgaa cacatttca cgtgatcct 
      gtttttctc tcgtatatt cctcagtgg gttcaatgt 
      acggcccca aaaatcgtc aagttcaat tatcccgca 
      tcgaaagtg caaaatacg gacactgga ggcgctgct 
      gcgtatgct aaaattgat tttgatagt gaggaagaa 
      ttttcaact tatttctat cgatgtcgg agcgatttt 
      cttgattcg tttcgtcaa gccaccgtt gtggcgcca 
      cttgtcact tttaattac gtcgaacag tggcttatg 
      aaatgtttg caagttcct aacgtcact tctgggctg 
      gttttgtcc gatccgctg tttcatgaa tgggaggct 
      ttgtccacc tttctggag agtatttta agtcgtgtt 
      cttcaggca caagaacgg ccttcaata gcatcgggt 
      ttgagatta ttacaatta tgtttggtt tatcagcct 
      gtcgatccg ttaatactt tcgactttg ttgacttgt 
      atttcggct ctatttgtt tttttgagt atgtcgact 
      ggtcaaatg gcaccgacg gccaattcg gtggcggcg 
      tccggggcg gccctatta ccacaagtt ttggataag 
      attttttcg actttggtg tatgcgcct cctgatgaa 
      cagagtaaa gatacaaga tcaagggct gtgaaaaac 
      gtacgaaga catgcagct tcgctgatg gttaaaatc 
      gggaataaa tatccgctt ttgttgttg cctgttttc 
      gatcagatt cgtgcaact gtggagaat ttgtcgaga 
      tcggatagt gtggctggg ttgagtacg ttggagaaa 
      gtgacgttg caggaggcg ctgcttttg atttctaat 
      catttttgt gattatgac cggcagagt aatttcgtg 
      agggaggtg ctagctgag gcgaacgcc caatggcgt 
      cttatcgtg gcgtctggc gcgttcgaa agcgcctcg 
      aaattcatc tcatttgtc ggtcttgac acaccccct 
      gtagcccca cacgctgac aatcctcac ggccataac 
      cgcagcagt atcgtcttc tgcatcaat cttctctta 
      ggtgccatt aaacgttgc tcgtggccg gaagatccc 
      gaacgtgcg actcgtggc ggtttcgtt gtagctctc 
      accgaatcg gggaatcct gtttgtcga aatccggct 
      gctccacac gttgtacct cttctacca gacattctt 
      tccctcatt cgcgttttc aacgaattg tttacgtgc 
      gaagctcag aatttgatt catgagagc tataaagga 
      tgtctcggg atgctcgag acagagaaa tcgaatctt 
      ctgggtttg attggacat tcggtgggt gatttgggc 
      gaattacaa gcggtacaa agtccgatg gagaggatg 
      cagaggttt ttgtttgga ttgcacgag agttgttat 
      catatgatt ggttcgatg gggccttcg ttgggcagg 
      gatttgtat actttgccc gatattggt ttagcaata 
      attaacagc gtcttggca tgtttacag tgtatccca 
      gattatcga atgcgaccc ataattcgt gttttctta 
      aaacccttt atctattct tgtcctacg ccattctac 
      gaagctgta cttttgccc atcgttgca cacatagca 
      cctctaatg ttatcacgt ttacacgca aaatggtta 
      caagtgaac gaatttcgc aatcgcgaa ggacaagaa 
      gataacgct gacactcaa gaagttctt gaagatatt 
      ttaacacga gctctaact cgtgaatat ttggacgta 
      ctaaaggtg gctttagta ggcggtgga ctcacacct 
      gaaacaaat actgaaaat atggaaact gaagatctg 
      agtatggat tccccaaca ccgcctcct ccaacacga 
      tcgaatatg accacggag gttatatct gatttaggt 
      ttggtactc ttgcgtagc gagaaaact tgtcagtcg 
      attgtgttg gcagtactg ggtgcgttg tcatggatt 
      gacagtaac gcatcgtta aaagcaact tttttgact 
      ggacctata gttagacaa ttggtttct gatagttct 
      ttaaacgga gagatggca gcacatata atggcgtcc 
      gttttgaac gctttgatg ttacatggg caacatgaa 
      gccaatcaa gggtcttta ttgacctta ggtgcacaa 
      atgtatgaa atgttacgg cctactttt ctcgaagta 
      ctcggtgtg atgcaacaa attcccgga gtgaaccca 
      gttgatttg caaaagttg gatgaaaga atttcaggc 
      agtacaagt aaaggaaat aaggtcgag aaggtgaag 
      aaggatttg tttcggaaa ataacggga aatttgatt 
      ggacgaagt atgggccag ctttttaaa aaagaggtc 
      aaaattcat gacctaccc agcttagca ttttcgaag 
      aaacctcaa ccgaaagaa gtgacacct gacttgcaa 
      aatgtgttt agttaa
    mRNA sequence
    • >TC008488 RA
      ctaaaaaac tgtaaatat tttggttca agtttgtgg 
      aaaactcca ccagatgcc gcacacgcc ggcaatggc 
      cggcaaccg tcaatgagc aaattatta gtgaaacat 
      catttgttc gaaaatcgt gtcatggac tagaaataa 
      ctcaaaaaa atcaacttt tttctccga atttttcga 
      aaacgatgg ctggaccag acgttgcgg ctcttgctg 
      cggatttag caagagccg ttgaactca caatgagca 
      ccggagctt cgcaaaccg accgactca aagcataca 
      acgcgtgcg aaagcttta aagagactt cacctctgt 
      gtgccgaag ctgggctct atttggccg cagggacgc 
      aacattcct tgatttcga gacatttcg gcttgcagt 
      tgatggagc atacggtca agtatcgct ggacgcaaa 
      tttcccaac aggagaaga tcttcatca aggaaaatg 
      caatgaagt tgcttgctg ctggaggga ttagtgacg 
      aaccgcaca tgaaggatg ctttaagtc gagttattg 
      tggaaatgg tgaagagag aatggccac aacaatggc 
      caggtttgc tctcagagc tgtcggaag cttgttctt 
      gtggggaga ttcaaactg aattggttt tgttggtgt 
      ttctacgat tagttgagg atgttgctt tactgcaga 
      cgctggaat caaatcagc gaagaaaag acatctatc 
      atgccctca ccgccaata tggctgtca tctttgatt 
      tttttcttc gtctaatcg aattacacg tgaaccaat 
      ttcgtatat gtggtgaaa caaataaca caccgaaat 
      ctaccgctc atggtagag tcgttcaag ttgttttac 
      tgactttga ctggttttg ttgaatggg tctctatgt 
      cacacatta tggctcaaa atggaagac tcctacaca 
      ttctgtgtc tgttattga acgatttgg cgtttcagt 
      atccggcgg ctgaatgtt tatcacaga ttgtaaata 
      gaaaaggta aagttgacg aacgaaaac cactcttgc 
      tcctcttca acgacgaac caatccaat gtttagttt 
      cggcgtcaa aaaatcccg gtgccattc tcgacgaac 
      aacactatt tatttaaga agaaacttg tacaggttt 
      taggcggtt taacaactc aacttgttg tcttatggg 
      gtaaagatt ctatttcac gtcccaaca acttttcgg 
      cttttttgg aagcaattc ttgcattta gtagccatc 
      aaagtctca cattatcac atatggcca accctctgt 
      ggaacagta tgctcaaac acgaacaca tttcacgtg 
      atcctgttt ttctctcgt atattcctc agtgggttc 
      aatgtacgg ccccaaaaa tcgtcaagt tcaattatc 
      ccgcatcga aagtgcaaa atacggaca ctggaggcg 
      ctgctgcgt atgctaaaa ttgattttg atagtgagg 
      aagaatttt caacttatt tctatcgat gtcggagcg 
      attttcttg attcgtttc gtcaagcca ccgttgtgg 
      cgccacttg tcactttta attacgtcg aacagtggc 
      ttatgaaat gtttgcaag ttcctaacg tcacttctg 
      ggctggttt tgtccgatc cgctgtttc atgaatggg 
      aggctttgt ccacctttc tggagagta ttttaagtc 
      gtgttcttc aggcacaag aacggcctt caatagcat 
      cgggtttga gattattac aattatgtt tggtttatc 
      agcctgtcg atccgttaa tactttcga ctttgttga 
      cttgtattt cggctctat ttgtttttt tgagtatgt 
      cgactggtc aaatggcac cgacggcca attcggtgg 
      cggcgtccg gggcggccc tattaccac aagttttgg 
      ataagattt tttcgactt tggtgtatg cgcctcctg 
      atgaacaga gtaaagata caagatcaa gggctgtga 
      aaaacgtac gaagacatg cagcttcgc tgatggtta 
      aaatcggga ataaatatc cgcttttgt tgttgcctg 
      ttttcgatc agattcgtg caactgtgg agaatttgt 
      cgagatcgg atagtgtgg ctgggttga gtacgttgg 
      agaaagtga cgttgcagg aggcgctgc ttttgattt 
      ctaatcatt tttgtgatt atgaccggc agagtaatt 
      tcgtgaggg aggtgctag ctgaggcga acgcccaat 
      ggcgtctta tcgtggcgt ctggcgcgt tcgaaagcg 
      cctcgaaat tcatctcat ttgtcggtc ttgacacac 
      cccctgtag ccccacacg ctgacaatc ctcacggcc 
      ataaccgca gcagtatcg tcttctgca tcaatcttc 
      tcttaggtg ccattaaac gttgctcgt ggccggaag 
      atcccgaac gtgcgactc gtggcggtt tcgttgtag 
      ctctcaccg aatcgggga atcctgttt gtcgaaatc 
      cggctgctc cacacgttg tacctcttc taccagaca 
      ttctttccc tcattcgcg ttttcaacg aattgttta 
      cgtgcgaag ctcagaatt tgattcatg agagctata 
      aaggatgtc tcgggatgc tcgagacag agaaatcga 
      atcttctgg gtttgattg gacattcgg tgggtgatt 
      tgggcgaat tacaagcgg tacaaagtc cgatggaga 
      ggatgcaga ggtttttgt ttggattgc acgagagtt 
      gttatcata tgattggtt cgatggggc cttcgttgg 
      gcagggatt tgtatactt tgcccgata ttggtttag 
      caataatta acagcgtct tggcatgtt tacagtgta 
      tcccagatt atcgaatgc gacccataa ttcgtgttt 
      tcttaaaac cctttatct attcttgtc ctacgccat 
      tctacgaag ctgtacttt tgcccatcg ttgcacaca 
      tagcacctc taatgttat cacgtttac acgcaaaat 
      ggttacaag tgaacgaat ttcgcaatc gcgaaggac 
      aagaagata acgctgaca ctcaagaag ttcttgaag 
      atattttaa cacgagctc taactcgtg aatatttgg 
      acgtactaa aggtggctt tagtaggcg gtggactca 
      cacctgaaa caaatactg aaaatatgg aaactgaag 
      atctgagta tggattccc caacaccgc ctcctccaa 
      cacgatcga atatgacca cggaggtta tatctgatt 
      taggtttgg tactcttgc gtagcgaga aaacttgtc 
      agtcgattg tgttggcag tactgggtg cgttgtcat 
      ggattgaca gtaacgcat cgttaaaag caacttttt 
      tgactggac ctatagtta gacaattgg tttctgata 
      gttctttaa acggagaga tggcagcac atataatgg 
      cgtccgttt tgaacgctt tgatgttac atgggcaac 
      atgaagcca atcaagggt ctttattga ccttaggtg 
      cacaaatgt atgaaatgt tacggccta cttttctcg 
      aagtactcg gtgtgatgc aacaaattc ccggagtga 
      acccagttg atttgcaaa agttggatg aaagaattt 
      caggcagta caagtaaag gaaataagg tcgagaagg 
      tgaagaagg atttgtttc ggaaaataa cgggaaatt 
      tgattggac gaagtatgg gccagcttt ttaaaaaag 
      aggtcaaaa ttcatgacc tacccagct tagcatttt 
      cgaagaaac ctcaaccga aagaagtga cacctgact 
      tgcaaaatg tgtttagtt aatattgcc tgaaatatt 
      atctaattg ttatttatt taatttatt gtacaacat 
      tccttgttg ttcaattgg tcaagaccg actttatca 
      tgatcaatc aatgcattc ttttgtaat atattgttg 
      taatttaat attatttac aatcttatc tcttggtct 
      ctaataaat agtaatttt tcaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY