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Gene information
General information
TC003437 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5166807..5168378
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003437
    iBeetle screen iB_10615

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber potentially number increased - Number of animals showing the phenotype: 3

    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 3

    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003437 RA
      MKLATKIGL TTATIITAY LIGNYLLTP TIPKLENTW 
      WGSRDPSTE DTTIKPFRI NVSEEVLED LQNRLANTK 
      LTPPLEGIQ HQYGLNTKL LSKIVTYWR TKYDWKKRE 
      IFLNKYPQF MVSIQGLRL HYIHLKPQK RDNVKTLPI 
      LLLHGWPGS VREFYTTIQ LLTKPTKGR NFIFEVIVP 
      SLPGFGFSE ASVRPGLGP IEMAVIFKN LMKRLGHEK 
      YLVQGGDWG ALIAQQMAV LFPDQVLGI HSNLCFDTS 
      VLSALKLAL GSLYPSWVV PKNFEHRLY PLLSFVMNL 
      VEETGYMHI QTSKPDTVG AALNDSPVG LAAYILEKF 
      ISWTNPDWK NFEDGGLLK KYTYDDLLD NIMIYWISG 
      SITTSMRLY SHALDNVMT IINVQRISI TVPSACAIF 
      PNEIFPQPG AMLREKYQN LVHVSENSD GGHFPAFEL 
      PHVYAEDIF VATEKMLEA NSQRN
    CDS sequence
    • >TC003437 RA
      atgaaactt gccacaaaa attggtcta acaactgca 
      acaattata acagcgtat ctaattgga aattattta 
      ctaaccccg acaattccc aaattagaa aacacctgg 
      tggggctca cgtgaccct tcaaccgaa gacacaaca 
      attaaacca ttccgaatt aacgtttct gaagaggta 
      ctcgaagat cttcaaaat cgcttagcc aacacaaaa 
      ctgacacca cctctagaa ggcattcag caccaatac 
      ggcctaaac accaaactt ttgagcaaa attgtgact 
      tactggcgc acgaaatac gactggaaa aagcgcgaa 
      attttctta aacaaatat ccccaattt atggtaagc 
      atccaaggc ttacgccta cactacatc cacctaaaa 
      ccccaaaaa agggacaat gttaaaacc ctccccatt 
      ctcctgctc cacggttgg cctggatca gtccgcgag 
      ttttacacc acaatccag ctgttaaca aaacccacc 
      aaagggcgc aactttatt ttcgaagtc atagttcca 
      tcactcccc ggttttggt ttttcggaa gcgtcggtt 
      cgcccaggt cttgggcca attgaaatg gccgtgatt 
      ttcaaaaac ttaatgaaa cggttgggg catgaaaaa 
      tatttagtg caagggggc gactggggg gcgcttatc 
      gcccaacaa atggcggtt ttgttcccg gatcaagtc 
      ctgggaatt cactccaat ctctgtttt gatacttcg 
      gttttgtct gctttgaaa ttagctttg gggagtttg 
      tacccttca tgggtggtt cccaaaaac ttcgaacac 
      agattatat cctctattg tctttcgtt atgaatttg 
      gtggaagaa acaggctac atgcacatt cagaccagt 
      aagcctgac actgttgga gcagctttg aatgattct 
      ccggtgggt ttggccgct tatatttta gaaaaattc 
      atttcttgg actaaccct gactggaaa aattttgaa 
      gatgggggg ttgttgaaa aaatacacc tatgacgac 
      cttttagac aatattatg atttattgg attagtggg 
      agtataacg acttcaatg agactctat tctcatgct 
      ctcgataac gtaatgaca ataataaat gttcaaagg 
      atttctata accgtacca agtgcttgt gcaatattt 
      cccaacgag attttccca caacctggt gcaatgtta 
      agagaaaag tatcagaat cttgttcat gtcagtgaa 
      aattcggat ggagggcat tttccagcg tttgaattg 
      ccgcatgtt tatgccgaa gatattttc gtcgcgacg 
      gagaagatg ctggaagct aattcccaa aggaattaa
    mRNA sequence
    • >TC003437 RA
      gtactgtgt tatcatcca ttccgaaac ggtcacaca 
      ctcaaattc aaacattta aaacatgaa acttgccac 
      aaaaattgg tctaacaac tgcaacaat tataacagc 
      gtatctaat tggaaatta tttactaac cccgacaat 
      tcccaaatt agaaaacac ctggtgggg ctcacgtga 
      cccttcaac cgaagacac aacaattaa accattccg 
      aattaacgt ttctgaaga ggtactcga agatcttca 
      aaatcgctt agccaacac aaaactgac accacctct 
      agaaggcat tcagcacca atacggcct aaacaccaa 
      acttttgag caaaattgt gacttactg gcgcacgaa 
      atacgactg gaaaaagcg cgaaatttt cttaaacaa 
      atatcccca atttatggt aagcatcca aggcttacg 
      cctacacta catccacct aaaacccca aaaaaggga 
      caatgttaa aaccctccc cattctcct gctccacgg 
      ttggcctgg atcagtccg cgagtttta caccacaat 
      ccagctgtt aacaaaacc caccaaagg gcgcaactt 
      tattttcga agtcatagt tccatcact ccccggttt 
      tggtttttc ggaagcgtc ggttcgccc aggtcttgg 
      gccaattga aatggccgt gattttcaa aaacttaat 
      gaaacggtt ggggcatga aaaatattt agtgcaagg 
      gggcgactg gggggcgct tatcgccca acaaatggc 
      ggttttgtt cccggatca agtcctggg aattcactc 
      caatctctg ttttgatac ttcggtttt gtctgcttt 
      gaaattagc tttggggag tttgtaccc ttcatgggt 
      ggttcccaa aaacttcga acacagatt atatcctct 
      attgtcttt cgttatgaa tttggtgga agaaacagg 
      ctacatgca cattcagac cagtaagcc tgacactgt 
      tggagcagc tttgaatga ttctccggt gggtttggc 
      cgcttatat tttagaaaa attcatttc ttggactaa 
      ccctgactg gaaaaattt tgaagatgg ggggttgtt 
      gaaaaaata cacctatga cgacctttt agacaatat 
      tatgattta ttggattag tgggagtat aacgacttc 
      aatgagact ctattctca tgctctcga taacgtaat 
      gacaataat aaatgttca aaggatttc tataaccgt 
      accaagtgc ttgtgcaat atttcccaa cgagatttt 
      cccacaacc tggtgcaat gttaagaga aaagtatca 
      gaatcttgt tcatgtcag tgaaaattc ggatggagg 
      gcattttcc agcgtttga attgccgca tgtttatgc 
      cgaagatat tttcgtcgc gacggagaa gatgctgga 
      agctaattc ccaaaggaa ttaaatttt tgttaagac 
      tttatttaa aaatataaa aatgtttga aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY