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Gene information
General information
TC014106 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5721175..5724755
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014106
    iBeetle screen iB_10608

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014106 RA
      MFTRRVSKF LINVNRCFH TSKNYSKDS EHPLLRTLR 
ILKEDFYRI EGPSKNELE KQRALMGLF PRHADVLII 
GGGAIGSSI AYWLKEKTS REGIRVIVI EKDMTYSKC 
TTVLSPGAL HQHFALPEN IQMSLFGAE FLRTIKKRF 
GNGVQVNFN PQGHLVLAD EAGAQHLVD NSVIEREFG 
VINELLSKE QLKNKFPWM NVEDVELGC LGLEKQGWF 
DPWALLSVM RYGATDLGA QYVEGEVVD FLFKDKQEY 
IGEGMEDAS YEAVQEAVI KLPDGQHKT MEFAFCIIA 
AGTETLELS QLARIGTGS GMLSIPLPI EKRKRFVYK 
FDCPESPPG INTPMTVDP SGLYFRRHG LGGSFLCGV 
SLPEKCDDS PEGFFNHQI SPVLQNRVP KFSDIKLRN 
SWCGIYENN YFDDSGIIG PHPYYHNLY IATGFSAYG 
IQQAPAIGR AVAELILDG DFKTIDLTR FGFDRLIVD 
KPMYEYRQI Q
    CDS sequence
    • >TC014106 RA
      atgtttact cgacgtgtc tcaaaattc ctaatcaat 
gtaaaccgg tgttttcac acatcaaag aattacagc 
aaagactct gaacaccca cttttgaga actctcaga 
atattgaaa gaagatttt taccgaatc gagggcccc 
tcgaaaaat gagcttgaa aagcaacgt gccctcatg 
ggccttttc cctcgacat gccgatgtt ttaatcatt 
ggcggaggg gccattggc tcttctatt gcttactgg 
ttaaaagaa aaaaccagt agagagggc ataagggtt 
atagttatt gaaaaagac atgacgtat tcaaaatgc 
accacagta ttgtcacca ggagcatta caccaacac 
tttgcattg cccgaaaat atacaaatg tcgcttttc 
ggagctgag tttcttcga acaattaaa aaaaggttc 
gggaacggg gtccaagtt aatttcaac cctcagggg 
cacttggtt ctggcggat gaagcaggt gcgcaacac 
cttgttgat aacagtgtc atagaaaga gaatttggt 
gtcattaac gagttactg agtaaagaa cagcttaaa 
aataagttt ccgtggatg aacgttgaa gatgtggaa 
ctgggttgt ttagggctg gaaaaacaa ggctggttt 
gacccttgg gctctgctc tcagtgatg cgatatggt 
gccactgac ttgggcgct cagtacgtg gaaggagaa 
gttgttgac ttccttttc aaggataag caagaatac 
attggggag ggtatggaa gatgcttca tatgaagct 
gtccaagaa gcagtgata aaattacca gacggccaa 
cacaaaacc atggaattc gctttctgt atcatagct 
gccggcact gagactctc gaattatca caacttgcg 
agaatcgga acaggctca gggatgtta tcaataccg 
cttccgatt gaaaaaaga aaaagattt gtttataaa 
tttgattgt ccagaatcg cctcctgga attaacact 
cccatgaca gtcgatccc tcagggctc tatttccgc 
cgacatggt ctcggtggt tctttctta tgcggcgta 
tcacttcca gaaaagtgt gacgattcg cctgagggt 
tttttcaat caccaaatt tcaccagtt ttgcagaac 
agagtcccc aaattcagc gatattaaa ctgagaaat 
tcctggtgc ggaatttac gaaaataat tattttgac 
gacagtgga ataataggc cctcatccc tactaccac 
aatttgtat atagcgaca ggttttagc gcttatggc 
attcagcaa gctccagcg attggccgg gctgtagcc 
gagcttatt ctggacggt gatttcaaa accatcgac 
ttgactagg tttggcttt gatcgatta atagtagat 
aagccaatg tacgaatat aggcaaatt caataa
    mRNA sequence
    • >TC014106 RA
      taaattatt gaaaataaa tgtgacagt gacataacg 
actaacctc aaaattact tttaaatgc tggcgtaat 
taattactc aattcatac ttattcgtg aatattaca 
atgaaaaag tgtagtttt ttttaaagt ttgaatttt 
tcaatgcca taaaatgtt tactcgacg tgtctcaaa 
attcctaat caatgtaaa ccggtgttt tcacacatc 
aaagaatta cagcaaaga ctctgaaca cccactttt 
gagaactct cagaatatt gaaagaaga tttttaccg 
aatcgaggg cccctcgaa aaatgagct tgaaaagca 
acgtgccct catgggcct tttccctcg acatgccga 
tgttttaat cattggcgg aggggccat tggctcttc 
tattgctta ctggttaaa agaaaaaac cagtagaga 
gggcataag ggttatagt tattgaaaa agacatgac 
gtattcaaa atgcaccac agtattgtc accaggagc 
attacacca acactttgc attgcccga aaatataca 
aatgtcgct tttcggagc tgagtttct tcgaacaat 
taaaaaaag gttcgggaa cggggtcca agttaattt 
caaccctca ggggcactt ggttctggc ggatgaagc 
aggtgcgca acaccttgt tgataacag tgtcataga 
aagagaatt tggtgtcat taacgagtt actgagtaa 
agaacagct taaaaataa gtttccgtg gatgaacgt 
tgaagatgt ggaactggg ttgtttagg gctggaaaa 
acaaggctg gtttgaccc ttgggctct gctctcagt 
gatgcgata tggtgccac tgacttggg cgctcagta 
cgtggaagg agaagttgt tgacttcct tttcaagga 
taagcaaga atacattgg ggagggtat ggaagatgc 
ttcatatga agctgtcca agaagcagt gataaaatt 
accagacgg ccaacacaa aaccatgga attcgcttt 
ctgtatcat agctgccgg cactgagac tctcgaatt 
atcacaact tgcgagaat cggaacagg ctcagggat 
gttatcaat accgcttcc gattgaaaa aagaaaaag 
atttgttta taaatttga ttgtccaga atcgcctcc 
tggaattaa cactcccat gacagtcga tccctcagg 
gctctattt ccgccgaca tggtctcgg tggttcttt 
cttatgcgg cgtatcact tccagaaaa gtgtgacga 
ttcgcctga gggtttttt caatcacca aatttcacc 
agttttgca gaacagagt ccccaaatt cagcgatat 
taaactgag aaattcctg gtgcggaat ttacgaaaa 
taattattt tgacgacag tggaataat aggccctca 
tccctacta ccacaattt gtatatagc gacaggttt 
tagcgctta tggcattca gcaagctcc agcgattgg 
ccgggctgt agccgagct tattctgga cggtgattt 
caaaaccat cgacttgac taggtttgg ctttgatcg 
attaatagt agataagcc aatgtacga atataggca 
aattcaata aaaagagct cctcaaatg aaacacgtt 
ttatcctac aaattctat atcttacat aacaaaaac 
aaaatgaaa tattacaaa atatacaaa caacattca 
catacatag tctatgatt acctcctaa aaatcttat 
tatttataa aagtacaaa aaatcccaa aaatatttg 
caaaaaaat agggatatt tccgtttct tgtttataa 
aactattac tattttcga caatggttt gtacaacaa 
gaaaactaa atgaattgc gtgttcatt gctaaacta 
acaaatata cagaataaa gtcactaaa ttacgctat 
tagtattta catgaataa ttaactaat atggcattg 
ttactggca taaaaatgc gtaacttaa gactattaa 
agacttcgt ttatgaacc agctttgtg atacaatgt 
gttctagta atatccctg agtattcaa ctaaaacta 
tacttttca ctctaacat tgtacattt agcaaaatt 
ttaagcttc acgcatttt taaccatga aaaccttcg 
catgcacct aattaaaca accttaccg tcaacaaac 
gtcggtttt ttctgttat aaaatctga ctagaggca 
atagaaaca taattgagc atattgcag tggcagtga 
gactgtaat aactacaat aaatgaaac aactaaaca 
aaaaagtat taccctaaa taaatgtaa aattgcgaa 
aatacgcca tactcactc aaatcgaac acaatcatg 
ttgatattt ttaaagctt taatacaat taaccacca 
atcaaagga gtcttttcg ggcaaaaat taccgaccc 
gctgacagt tttacacaa taaaactat aacgataaa 
aagtataaa gagataaat ttgcatgat tttgtaaat 
ggtgtgtag aatcaaata aaaattgtt cttccactt 
aaaattgca tacaatcaa agaagtaca taatataat 
aatcacgtg aaaatttta ctaatcgtc ataaaactc 
gccaatatt ttaaacatt acttcttta attataagc 
agttagtaa aagttaaaa ttacatgga atacgtgta 
ttataatat attagtcga caggatatg cctggacat 
atttaaata ttaagtacg gttaaaacg gagatcata 
ttacatctc taacaataa ataaaaact ttcaattct 
tacaattcg acaattcca acgttcatg atgaatacg 
gctgttttg tctataaag tctgaaaaa cgaatcaca 
actcgtttt tcagtcttt acgtaaaac caacttgtg 
aaaatactt tcactgtac aatgatcga ggataatca 
acaaattat gaattgagt gatttgtca atgcaagtc 
tagaagtct cgttatgct aaacgacgg atccacata 
ctctcagct aggtttcga tgttgactg atggtatat 
atctgagtc tcttctaat taccatctg cgaaacaaa 
tcttcactg aggaaaggc aagcaaact cggcacaac 
atgcataaa aaagcaatg gaaaaatgg aacagtatt 
tcgatgtta gtagcgctt gctgttaat gaagcaaca 
aaaaacaac gagaaggaa cactttaca acgcacaaa 
ataaactaa tagtaaaag a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY