| General information | |||
| TC014106 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 5721175..5724755 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014106 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10608 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014106 RA
MFTRRVSKF LINVNRCFH TSKNYSKDS EHPLLRTLR ILKEDFYRI EGPSKNELE KQRALMGLF PRHADVLII GGGAIGSSI AYWLKEKTS REGIRVIVI EKDMTYSKC TTVLSPGAL HQHFALPEN IQMSLFGAE FLRTIKKRF GNGVQVNFN PQGHLVLAD EAGAQHLVD NSVIEREFG VINELLSKE QLKNKFPWM NVEDVELGC LGLEKQGWF DPWALLSVM RYGATDLGA QYVEGEVVD FLFKDKQEY IGEGMEDAS YEAVQEAVI KLPDGQHKT MEFAFCIIA AGTETLELS QLARIGTGS GMLSIPLPI EKRKRFVYK FDCPESPPG INTPMTVDP SGLYFRRHG LGGSFLCGV SLPEKCDDS PEGFFNHQI SPVLQNRVP KFSDIKLRN SWCGIYENN YFDDSGIIG PHPYYHNLY IATGFSAYG IQQAPAIGR AVAELILDG DFKTIDLTR FGFDRLIVD KPMYEYRQI Q
- >TC014106 RA
atgtttact cgacgtgtc tcaaaattc ctaatcaat gtaaaccgg tgttttcac acatcaaag aattacagc aaagactct gaacaccca cttttgaga actctcaga atattgaaa gaagatttt taccgaatc gagggcccc tcgaaaaat gagcttgaa aagcaacgt gccctcatg ggccttttc cctcgacat gccgatgtt ttaatcatt ggcggaggg gccattggc tcttctatt gcttactgg ttaaaagaa aaaaccagt agagagggc ataagggtt atagttatt gaaaaagac atgacgtat tcaaaatgc accacagta ttgtcacca ggagcatta caccaacac tttgcattg cccgaaaat atacaaatg tcgcttttc ggagctgag tttcttcga acaattaaa aaaaggttc gggaacggg gtccaagtt aatttcaac cctcagggg cacttggtt ctggcggat gaagcaggt gcgcaacac cttgttgat aacagtgtc atagaaaga gaatttggt gtcattaac gagttactg agtaaagaa cagcttaaa aataagttt ccgtggatg aacgttgaa gatgtggaa ctgggttgt ttagggctg gaaaaacaa ggctggttt gacccttgg gctctgctc tcagtgatg cgatatggt gccactgac ttgggcgct cagtacgtg gaaggagaa gttgttgac ttccttttc aaggataag caagaatac attggggag ggtatggaa gatgcttca tatgaagct gtccaagaa gcagtgata aaattacca gacggccaa cacaaaacc atggaattc gctttctgt atcatagct gccggcact gagactctc gaattatca caacttgcg agaatcgga acaggctca gggatgtta tcaataccg cttccgatt gaaaaaaga aaaagattt gtttataaa tttgattgt ccagaatcg cctcctgga attaacact cccatgaca gtcgatccc tcagggctc tatttccgc cgacatggt ctcggtggt tctttctta tgcggcgta tcacttcca gaaaagtgt gacgattcg cctgagggt tttttcaat caccaaatt tcaccagtt ttgcagaac agagtcccc aaattcagc gatattaaa ctgagaaat tcctggtgc ggaatttac gaaaataat tattttgac gacagtgga ataataggc cctcatccc tactaccac aatttgtat atagcgaca ggttttagc gcttatggc attcagcaa gctccagcg attggccgg gctgtagcc gagcttatt ctggacggt gatttcaaa accatcgac ttgactagg tttggcttt gatcgatta atagtagat aagccaatg tacgaatat aggcaaatt caataa
- >TC014106 RA
taaattatt gaaaataaa tgtgacagt gacataacg actaacctc aaaattact tttaaatgc tggcgtaat taattactc aattcatac ttattcgtg aatattaca atgaaaaag tgtagtttt ttttaaagt ttgaatttt tcaatgcca taaaatgtt tactcgacg tgtctcaaa attcctaat caatgtaaa ccggtgttt tcacacatc aaagaatta cagcaaaga ctctgaaca cccactttt gagaactct cagaatatt gaaagaaga tttttaccg aatcgaggg cccctcgaa aaatgagct tgaaaagca acgtgccct catgggcct tttccctcg acatgccga tgttttaat cattggcgg aggggccat tggctcttc tattgctta ctggttaaa agaaaaaac cagtagaga gggcataag ggttatagt tattgaaaa agacatgac gtattcaaa atgcaccac agtattgtc accaggagc attacacca acactttgc attgcccga aaatataca aatgtcgct tttcggagc tgagtttct tcgaacaat taaaaaaag gttcgggaa cggggtcca agttaattt caaccctca ggggcactt ggttctggc ggatgaagc aggtgcgca acaccttgt tgataacag tgtcataga aagagaatt tggtgtcat taacgagtt actgagtaa agaacagct taaaaataa gtttccgtg gatgaacgt tgaagatgt ggaactggg ttgtttagg gctggaaaa acaaggctg gtttgaccc ttgggctct gctctcagt gatgcgata tggtgccac tgacttggg cgctcagta cgtggaagg agaagttgt tgacttcct tttcaagga taagcaaga atacattgg ggagggtat ggaagatgc ttcatatga agctgtcca agaagcagt gataaaatt accagacgg ccaacacaa aaccatgga attcgcttt ctgtatcat agctgccgg cactgagac tctcgaatt atcacaact tgcgagaat cggaacagg ctcagggat gttatcaat accgcttcc gattgaaaa aagaaaaag atttgttta taaatttga ttgtccaga atcgcctcc tggaattaa cactcccat gacagtcga tccctcagg gctctattt ccgccgaca tggtctcgg tggttcttt cttatgcgg cgtatcact tccagaaaa gtgtgacga ttcgcctga gggtttttt caatcacca aatttcacc agttttgca gaacagagt ccccaaatt cagcgatat taaactgag aaattcctg gtgcggaat ttacgaaaa taattattt tgacgacag tggaataat aggccctca tccctacta ccacaattt gtatatagc gacaggttt tagcgctta tggcattca gcaagctcc agcgattgg ccgggctgt agccgagct tattctgga cggtgattt caaaaccat cgacttgac taggtttgg ctttgatcg attaatagt agataagcc aatgtacga atataggca aattcaata aaaagagct cctcaaatg aaacacgtt ttatcctac aaattctat atcttacat aacaaaaac aaaatgaaa tattacaaa atatacaaa caacattca catacatag tctatgatt acctcctaa aaatcttat tatttataa aagtacaaa aaatcccaa aaatatttg caaaaaaat agggatatt tccgtttct tgtttataa aactattac tattttcga caatggttt gtacaacaa gaaaactaa atgaattgc gtgttcatt gctaaacta acaaatata cagaataaa gtcactaaa ttacgctat tagtattta catgaataa ttaactaat atggcattg ttactggca taaaaatgc gtaacttaa gactattaa agacttcgt ttatgaacc agctttgtg atacaatgt gttctagta atatccctg agtattcaa ctaaaacta tacttttca ctctaacat tgtacattt agcaaaatt ttaagcttc acgcatttt taaccatga aaaccttcg catgcacct aattaaaca accttaccg tcaacaaac gtcggtttt ttctgttat aaaatctga ctagaggca atagaaaca taattgagc atattgcag tggcagtga gactgtaat aactacaat aaatgaaac aactaaaca aaaaagtat taccctaaa taaatgtaa aattgcgaa aatacgcca tactcactc aaatcgaac acaatcatg ttgatattt ttaaagctt taatacaat taaccacca atcaaagga gtcttttcg ggcaaaaat taccgaccc gctgacagt tttacacaa taaaactat aacgataaa aagtataaa gagataaat ttgcatgat tttgtaaat ggtgtgtag aatcaaata aaaattgtt cttccactt aaaattgca tacaatcaa agaagtaca taatataat aatcacgtg aaaatttta ctaatcgtc ataaaactc gccaatatt ttaaacatt acttcttta attataagc agttagtaa aagttaaaa ttacatgga atacgtgta ttataatat attagtcga caggatatg cctggacat atttaaata ttaagtacg gttaaaacg gagatcata ttacatctc taacaataa ataaaaact ttcaattct tacaattcg acaattcca acgttcatg atgaatacg gctgttttg tctataaag tctgaaaaa cgaatcaca actcgtttt tcagtcttt acgtaaaac caacttgtg aaaatactt tcactgtac aatgatcga ggataatca acaaattat gaattgagt gatttgtca atgcaagtc tagaagtct cgttatgct aaacgacgg atccacata ctctcagct aggtttcga tgttgactg atggtatat atctgagtc tcttctaat taccatctg cgaaacaaa tcttcactg aggaaaggc aagcaaact cggcacaac atgcataaa aaagcaatg gaaaaatgg aacagtatt tcgatgtta gtagcgctt gctgttaat gaagcaaca aaaaacaac gagaaggaa cactttaca acgcacaaa ataaactaa tagtaaaag a
