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Gene information
General information
TC014106 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 5721175..5724755
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014106
    iBeetle screen iB_10608

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014106 RA
      MFTRRVSKF LINVNRCFH TSKNYSKDS EHPLLRTLR 
      ILKEDFYRI EGPSKNELE KQRALMGLF PRHADVLII 
      GGGAIGSSI AYWLKEKTS REGIRVIVI EKDMTYSKC 
      TTVLSPGAL HQHFALPEN IQMSLFGAE FLRTIKKRF 
      GNGVQVNFN PQGHLVLAD EAGAQHLVD NSVIEREFG 
      VINELLSKE QLKNKFPWM NVEDVELGC LGLEKQGWF 
      DPWALLSVM RYGATDLGA QYVEGEVVD FLFKDKQEY 
      IGEGMEDAS YEAVQEAVI KLPDGQHKT MEFAFCIIA 
      AGTETLELS QLARIGTGS GMLSIPLPI EKRKRFVYK 
      FDCPESPPG INTPMTVDP SGLYFRRHG LGGSFLCGV 
      SLPEKCDDS PEGFFNHQI SPVLQNRVP KFSDIKLRN 
      SWCGIYENN YFDDSGIIG PHPYYHNLY IATGFSAYG 
      IQQAPAIGR AVAELILDG DFKTIDLTR FGFDRLIVD 
      KPMYEYRQI Q
    CDS sequence
    • >TC014106 RA
      atgtttact cgacgtgtc tcaaaattc ctaatcaat 
      gtaaaccgg tgttttcac acatcaaag aattacagc 
      aaagactct gaacaccca cttttgaga actctcaga 
      atattgaaa gaagatttt taccgaatc gagggcccc 
      tcgaaaaat gagcttgaa aagcaacgt gccctcatg 
      ggccttttc cctcgacat gccgatgtt ttaatcatt 
      ggcggaggg gccattggc tcttctatt gcttactgg 
      ttaaaagaa aaaaccagt agagagggc ataagggtt 
      atagttatt gaaaaagac atgacgtat tcaaaatgc 
      accacagta ttgtcacca ggagcatta caccaacac 
      tttgcattg cccgaaaat atacaaatg tcgcttttc 
      ggagctgag tttcttcga acaattaaa aaaaggttc 
      gggaacggg gtccaagtt aatttcaac cctcagggg 
      cacttggtt ctggcggat gaagcaggt gcgcaacac 
      cttgttgat aacagtgtc atagaaaga gaatttggt 
      gtcattaac gagttactg agtaaagaa cagcttaaa 
      aataagttt ccgtggatg aacgttgaa gatgtggaa 
      ctgggttgt ttagggctg gaaaaacaa ggctggttt 
      gacccttgg gctctgctc tcagtgatg cgatatggt 
      gccactgac ttgggcgct cagtacgtg gaaggagaa 
      gttgttgac ttccttttc aaggataag caagaatac 
      attggggag ggtatggaa gatgcttca tatgaagct 
      gtccaagaa gcagtgata aaattacca gacggccaa 
      cacaaaacc atggaattc gctttctgt atcatagct 
      gccggcact gagactctc gaattatca caacttgcg 
      agaatcgga acaggctca gggatgtta tcaataccg 
      cttccgatt gaaaaaaga aaaagattt gtttataaa 
      tttgattgt ccagaatcg cctcctgga attaacact 
      cccatgaca gtcgatccc tcagggctc tatttccgc 
      cgacatggt ctcggtggt tctttctta tgcggcgta 
      tcacttcca gaaaagtgt gacgattcg cctgagggt 
      tttttcaat caccaaatt tcaccagtt ttgcagaac 
      agagtcccc aaattcagc gatattaaa ctgagaaat 
      tcctggtgc ggaatttac gaaaataat tattttgac 
      gacagtgga ataataggc cctcatccc tactaccac 
      aatttgtat atagcgaca ggttttagc gcttatggc 
      attcagcaa gctccagcg attggccgg gctgtagcc 
      gagcttatt ctggacggt gatttcaaa accatcgac 
      ttgactagg tttggcttt gatcgatta atagtagat 
      aagccaatg tacgaatat aggcaaatt caataa
    mRNA sequence
    • >TC014106 RA
      taaattatt gaaaataaa tgtgacagt gacataacg 
      actaacctc aaaattact tttaaatgc tggcgtaat 
      taattactc aattcatac ttattcgtg aatattaca 
      atgaaaaag tgtagtttt ttttaaagt ttgaatttt 
      tcaatgcca taaaatgtt tactcgacg tgtctcaaa 
      attcctaat caatgtaaa ccggtgttt tcacacatc 
      aaagaatta cagcaaaga ctctgaaca cccactttt 
      gagaactct cagaatatt gaaagaaga tttttaccg 
      aatcgaggg cccctcgaa aaatgagct tgaaaagca 
      acgtgccct catgggcct tttccctcg acatgccga 
      tgttttaat cattggcgg aggggccat tggctcttc 
      tattgctta ctggttaaa agaaaaaac cagtagaga 
      gggcataag ggttatagt tattgaaaa agacatgac 
      gtattcaaa atgcaccac agtattgtc accaggagc 
      attacacca acactttgc attgcccga aaatataca 
      aatgtcgct tttcggagc tgagtttct tcgaacaat 
      taaaaaaag gttcgggaa cggggtcca agttaattt 
      caaccctca ggggcactt ggttctggc ggatgaagc 
      aggtgcgca acaccttgt tgataacag tgtcataga 
      aagagaatt tggtgtcat taacgagtt actgagtaa 
      agaacagct taaaaataa gtttccgtg gatgaacgt 
      tgaagatgt ggaactggg ttgtttagg gctggaaaa 
      acaaggctg gtttgaccc ttgggctct gctctcagt 
      gatgcgata tggtgccac tgacttggg cgctcagta 
      cgtggaagg agaagttgt tgacttcct tttcaagga 
      taagcaaga atacattgg ggagggtat ggaagatgc 
      ttcatatga agctgtcca agaagcagt gataaaatt 
      accagacgg ccaacacaa aaccatgga attcgcttt 
      ctgtatcat agctgccgg cactgagac tctcgaatt 
      atcacaact tgcgagaat cggaacagg ctcagggat 
      gttatcaat accgcttcc gattgaaaa aagaaaaag 
      atttgttta taaatttga ttgtccaga atcgcctcc 
      tggaattaa cactcccat gacagtcga tccctcagg 
      gctctattt ccgccgaca tggtctcgg tggttcttt 
      cttatgcgg cgtatcact tccagaaaa gtgtgacga 
      ttcgcctga gggtttttt caatcacca aatttcacc 
      agttttgca gaacagagt ccccaaatt cagcgatat 
      taaactgag aaattcctg gtgcggaat ttacgaaaa 
      taattattt tgacgacag tggaataat aggccctca 
      tccctacta ccacaattt gtatatagc gacaggttt 
      tagcgctta tggcattca gcaagctcc agcgattgg 
      ccgggctgt agccgagct tattctgga cggtgattt 
      caaaaccat cgacttgac taggtttgg ctttgatcg 
      attaatagt agataagcc aatgtacga atataggca 
      aattcaata aaaagagct cctcaaatg aaacacgtt 
      ttatcctac aaattctat atcttacat aacaaaaac 
      aaaatgaaa tattacaaa atatacaaa caacattca 
      catacatag tctatgatt acctcctaa aaatcttat 
      tatttataa aagtacaaa aaatcccaa aaatatttg 
      caaaaaaat agggatatt tccgtttct tgtttataa 
      aactattac tattttcga caatggttt gtacaacaa 
      gaaaactaa atgaattgc gtgttcatt gctaaacta 
      acaaatata cagaataaa gtcactaaa ttacgctat 
      tagtattta catgaataa ttaactaat atggcattg 
      ttactggca taaaaatgc gtaacttaa gactattaa 
      agacttcgt ttatgaacc agctttgtg atacaatgt 
      gttctagta atatccctg agtattcaa ctaaaacta 
      tacttttca ctctaacat tgtacattt agcaaaatt 
      ttaagcttc acgcatttt taaccatga aaaccttcg 
      catgcacct aattaaaca accttaccg tcaacaaac 
      gtcggtttt ttctgttat aaaatctga ctagaggca 
      atagaaaca taattgagc atattgcag tggcagtga 
      gactgtaat aactacaat aaatgaaac aactaaaca 
      aaaaagtat taccctaaa taaatgtaa aattgcgaa 
      aatacgcca tactcactc aaatcgaac acaatcatg 
      ttgatattt ttaaagctt taatacaat taaccacca 
      atcaaagga gtcttttcg ggcaaaaat taccgaccc 
      gctgacagt tttacacaa taaaactat aacgataaa 
      aagtataaa gagataaat ttgcatgat tttgtaaat 
      ggtgtgtag aatcaaata aaaattgtt cttccactt 
      aaaattgca tacaatcaa agaagtaca taatataat 
      aatcacgtg aaaatttta ctaatcgtc ataaaactc 
      gccaatatt ttaaacatt acttcttta attataagc 
      agttagtaa aagttaaaa ttacatgga atacgtgta 
      ttataatat attagtcga caggatatg cctggacat 
      atttaaata ttaagtacg gttaaaacg gagatcata 
      ttacatctc taacaataa ataaaaact ttcaattct 
      tacaattcg acaattcca acgttcatg atgaatacg 
      gctgttttg tctataaag tctgaaaaa cgaatcaca 
      actcgtttt tcagtcttt acgtaaaac caacttgtg 
      aaaatactt tcactgtac aatgatcga ggataatca 
      acaaattat gaattgagt gatttgtca atgcaagtc 
      tagaagtct cgttatgct aaacgacgg atccacata 
      ctctcagct aggtttcga tgttgactg atggtatat 
      atctgagtc tcttctaat taccatctg cgaaacaaa 
      tcttcactg aggaaaggc aagcaaact cggcacaac 
      atgcataaa aaagcaatg gaaaaatgg aacagtatt 
      tcgatgtta gtagcgctt gctgttaat gaagcaaca 
      aaaaacaac gagaaggaa cactttaca acgcacaaa 
      ataaactaa tagtaaaag a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY