Status
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Gene information
General information
TC009894 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 12628305..12643041
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009894
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10607

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009894 RA
      MKRPPGLWI LLSAGVLWL SLQDVYGIR ATALIFGKS 
      TTTTTSTEA TSEAETSPD ETATEASTE SSNVTKPTL 
      TGVPQVDYI WDPNLPKEL NGYNLSEYP FYERVPEDI 
      DFKCDGLHD GFYASVPHK CQVYHHCLF GTRYDFLCA 
      NYTAFDQKT FICHFVSEV DCVNSKKYW HRNDALYKA 
      ATTTTAKPV VVYTPPPQP HPAAAPAAV NPRRPGGRR 
      RPMRRRRPQ YDYYDYADD YDDYYEERP PRSEARRRK 
      RPRPRPRPV YEDDYEEYE DERYERRGP GRRRAGDRR 
      RNNNKDRRK YEDEDKYED DKLDDDRKS NDRRPNNKD 
      RRKNEDEED DKLEEDRKT EDKRTSERR HHKRRPPHD 
      YSEEEEEDR RRSRPKGGS RRPVEDDYE EEEAPQNDK 
      PSAPENVGD KPLIKPTSG SSIYDRPRA APKIKPPVP 
      KNEASKYAY KPVVTKAPP VEDEEYYDD YEETPPKTH 
      RKNERHTTR REEERERKP SPPRLRASQ SSRDDVSYE 
      EDVPRRSQK PKYGSHHRE RPRNKKPPP PVEDYYDDY 
      EEESKETPA KAEKPKEKS TTTTTTTTT TTTTTTTTP 
      TTTTTTTTT TTPPPPSTT TERELRPES IIRIVKRPF 
      LPSRGGNPY GPRGIQPVG AKAVEKKPE IKEEETSES 
      AKAAFKPSV VIKTPLRQK YVNEPQEEF APVSSRPQT 
      PPRPSLKPS KEFLGPRTT QKPKLPEPD PLDIEDEYD 
      VTLNDALNP TLPNLPVRG FPTGFSNAN NEYNNYNSY 
      QRPRYVLEG ANSDYYQAK PTRQRYEAV PYSNPQEHF 
      VNSEADYKG QYSTVNRAN FRPRLTQAL YTSF
    CDS sequence
    • >TC009894 RA
      atgaaaagg ccgcctggg ctttggatc ctactgtcg 
      gcaggtgtt ctctggctg agtctacaa gatgtttac 
      ggaatcaga gccacagcg ctcatcttt ggcaagtcg 
      acaacgacc acgacttcc acagaagcg acttcagaa 
      gctgagact tctccagat gagactgcc accgaggcg 
      agcaccgag tcctccaac gtgaccaag cccaccctc 
      acgggggtg ccccaggtg gactacatc tgggacccc 
      aacttgccc aaagagctc aacgggtac aacctctcc 
      gagtacccc ttctacgag agggtgccc gaagacatc 
      gacttcaag tgcgatggt ctccatgac gggttctac 
      gccagcgtg ccccacaaa tgccaggtc taccaccac 
      tgccttttc ggaacaaga tacgacttt ttatgtgcg 
      aattatacc gcatttgac cagaaaaca tttatctgc 
      cacttcgtg tccgaggtg gattgcgtc aattcgaag 
      aaatattgg cacaggaat gacgctctg tataaggca 
      gccacgacc acgactgcg aagcctgtg gtggtttat 
      acgccgccc ccgcaaccg caccctgca gccgcccct 
      gcggcggtg aacccccgg aggcctggg ggccgccgg 
      cggcctatg cgaagaagg cggccgcag tacgattat 
      tacgattac gccgacgat tatgacgat tattacgaa 
      gaaaggcct ccaaggtcg gaagcgaga agaagaaag 
      aggccacga ccgagacct agaccagtt tatgaagac 
      gactacgaa gaatacgag gatgagagg tacgaaaga 
      aggggaccg ggaaggagg cgagctggg gacaggcga 
      agaaacaac aataaagat agaagaaaa tacgaagat 
      gaggataaa tacgaagat gataagtta gacgacgat 
      agaaaatca aatgataga agaccgaat aataaagac 
      agaagaaaa aatgaagat gaagaagat gataaacta 
      gaagaggat agaaaaaca gaagataaa agaacgagc 
      gaaaggaga catcataaa aggcggcca ccgcatgat 
      tactcggag gaagaagag gaagataga agaagaagt 
      cgtcctaaa ggaggctct cgacggcca gttgaagat 
      gattacgaa gaggaagaa gcacctcaa aatgataaa 
      ccctcagcg cctgaaaat gtcggtgat aaaccactg 
      ataaaacca acaagtggt agttcaatt tatgataga 
      cctagagct gccccaaaa attaaacca ccagtccct 
      aaaaatgaa gcaagcaaa tatgcttat aaaccggta 
      gtgactaaa gcacctccg gttgaagat gaagaatac 
      tacgacgat tatgaagaa actccccct aaaacacac 
      cgaaaaaat gaacgacat acaaccaga cgtgaagag 
      gagagagaa cgaaaacca tcacctcca agattacga 
      gcatcgcag tcatctcga gacgatgta agttatgaa 
      gaagacgtc cctcgaaga agccagaaa ccgaaatat 
      gggtcacac catcgtgaa agacctagg aacaaaaaa 
      ccaccacca ccagttgaa gattattac gacgattat 
      gaagaagag agtaaggaa acacccgcg aaagcggaa 
      aaaccgaaa gaaaaatcg actacgaca acaaccacg 
      acgacaaca acaacgaca accacaacg acaacaccc 
      acaacaacg acaacgact actacaacg accacaccc 
      cctccacct agcacaaca acagaaagg gaattgcgt 
      cccgaatct ataattcgg atagtaaaa agaccgttt 
      ttgcccagc cggggcggt aatccctac ggcccccgt 
      ggcatccaa cctgtgggg gccaaagcg gtagaaaaa 
      aagcccgaa ataaaagag gaagaaact tctgaaagt 
      gctaaagcg gcatttaaa ccctctgtt gtaataaaa 
      acaccacta cgtcaaaaa tacgtaaac gaaccccaa 
      gaagaattt gcccccgtg tcaagtcgg ccccaaact 
      ccccctcgc ccaagtctc aaacctagc aaggaattc 
      ctaggaccg cgaacgact caaaaaccc aaattgcct 
      gaacccgat cctctggac atagaagat gaatatgac 
      gtcacctta aacgacgcc ctaaacccc actttgccc 
      aatttaccg gttcgggga ttccccacc gggtttagt 
      aacgctaat aacgagtat aataattac aattcgtat 
      cagagaccg aggtatgtc cttgaaggt gcaaattcg 
      gattattat caagccaag ccgacgagg caaagatac 
      gaagcggtg ccctacagt aacccgcag gaacatttt 
      gttaattct gaggccgat tacaaaggg cagtacagc 
      acagtcaac agagctaat ttcaggccc agactcacg 
      caagccctc tacacgagt ttctga
    mRNA sequence
    • >TC009894 RA
      tgggggtca aataaggtc cataaatgt cgtaagagg 
      ttacagggc ggccgtaaa tagcgacat tacataaac 
      cgattagtg tgtgcacta gctaattaa tgagcacat 
      aaacggccc cttattgtt ggaaagaga cagagagcc 
      cccatatcc caccttaga aagttgctc gagtggcac 
      cagcttgtc aaggtagcg gcaaagcgg tctttggca 
      ccgctactc cggtagaaa gtccccaaa ccgcaccgt 
      tcgaatcgc tcctccact agctgaaga attgaaaat 
      ttctgtctt cagtcggtt cacttggat tgttgcaga 
      caggtgaga atcggaaga gtgaataaa tcgcgtgcg 
      attttcgaa aaaaaaagt ggtgatggt cgaaaaccg 
      atgtcgcca cattagaaa actctgcta tttttcgaa 
      actgggcct gaaattgct gaagttcgg actgtatgc 
      catagtgcc gttccggct ctttcccgt caaccgatt 
      tttggcgaa aacgcttct gggggcgtg aaattgcgc 
      ctgtggggg gcttgttca gtggtgctc gagacgtct 
      ttctcattt atttgaaat atctgatat tgactattg 
      ttctgactt tagtgacct acttgtacc tgctttatt 
      ttacgagag acgatatca aactgtaat tagcgagtg 
      ttaaagtgt acagtgaaa tgcgttaaa ttttcaaat 
      taattcatt ggaaaacag ttgttctag ttaaacaca 
      ggaggatga aaaggccgc ctgggcttt ggatcctac 
      tgtcggcag gtgttctct ggctgagtc tacaagatg 
      tttacggaa tcagagcca cagcgctca tctttggca 
      agtcgacaa cgaccacga cttccacag aagcgactt 
      cagaagctg agacttctc cagatgaga ctgccaccg 
      aggcgagca ccgagtcct ccaacgtga ccaagccca 
      ccctcacgg gggtgcccc aggtggact acatctggg 
      accccaact tgcccaaag agctcaacg ggtacaacc 
      tctccgagt accccttct acgagaggg tgcccgaag 
      acatcgact tcaagtgcg atggtctcc atgacgggt 
      tctacgcca gcgtgcccc acaaatgcc aggtctacc 
      accactgcc ttttcggaa caagatacg actttttat 
      gtgcgaatt ataccgcat ttgaccaga aaacattta 
      tctgccact tcgtgtccg aggtggatt gcgtcaatt 
      cgaagaaat attggcaca ggaatgacg ctctgtata 
      aggcagcca cgaccacga ctgcgaagc ctgtggtgg 
      tttatacgc cgcccccgc aaccgcacc ctgcagccg 
      cccctgcgg cggtgaacc cccggaggc ctgggggcc 
      gccggcggc ctatgcgaa gaaggcggc cgcagtacg 
      attattacg attacgccg acgattatg acgattatt 
      acgaagaaa ggcctccaa ggtcggaag cgagaagaa 
      gaaagaggc cacgaccga gacctagac cagtttatg 
      aagacgact acgaagaat acgaggatg agaggtacg 
      aaagaaggg gaccgggaa ggaggcgag ctggggaca 
      ggcgaagaa acaacaata aagatagaa gaaaatacg 
      aagatgagg ataaatacg aagatgata agttagacg 
      acgatagaa aatcaaatg atagaagac cgaataata 
      aagacagaa gaaaaaatg aagatgaag aagatgata 
      aactagaag aggatagaa aaacagaag ataaaagaa 
      cgagcgaaa ggagacatc ataaaaggc ggccaccgc 
      atgattact cggaggaag aagaggaag atagaagaa 
      gaagtcgtc ctaaaggag gctctcgac ggccagttg 
      aagatgatt acgaagagg aagaagcac ctcaaaatg 
      ataaaccct cagcgcctg aaaatgtcg gtgataaac 
      cactgataa aaccaacaa gtggtagtt caatttatg 
      atagaccta gagctgccc caaaaatta aaccaccag 
      tccctaaaa atgaagcaa gcaaatatg cttataaac 
      cggtagtga ctaaagcac ctccggttg aagatgaag 
      aatactacg acgattatg aagaaactc cccctaaaa 
      cacaccgaa aaaatgaac gacatacaa ccagacgtg 
      aagaggaga gagaacgaa aaccatcac ctccaagat 
      tacgagcat cgcagtcat ctcgagacg atgtaagtt 
      atgaagaag acgtccctc gaagaagcc agaaaccga 
      aatatgggt cacaccatc gtgaaagac ctaggaaca 
      aaaaaccac caccaccag ttgaagatt attacgacg 
      attatgaag aagagagta aggaaacac ccgcgaaag 
      cggaaaaac cgaaagaaa aatcgacta cgacaacaa 
      ccacgacga caacaacaa cgacaacca caacgacaa 
      cacccacaa caacgacaa cgactacta caacgacca 
      caccccctc cacctagca caacaacag aaagggaat 
      tgcgtcccg aatctataa ttcggatag taaaaagac 
      cgtttttgc ccagccggg gcggtaatc cctacggcc 
      cccgtggca tccaacctg tgggggcca aagcggtag 
      aaaaaaagc ccgaaataa aagaggaag aaacttctg 
      aaagtgcta aagcggcat ttaaaccct ctgttgtaa 
      taaaaacac cactacgtc aaaaatacg taaacgaac 
      cccaagaag aatttgccc ccgtgtcaa gtcggcccc 
      aaactcccc ctcgcccaa gtctcaaac ctagcaagg 
      aattcctag gaccgcgaa cgactcaaa aacccaaat 
      tgcctgaac ccgatcctc tggacatag aagatgaat 
      atgacgtca ccttaaacg acgccctaa accccactt 
      tgcccaatt taccggttc ggggattcc ccaccgggt 
      ttagtaacg ctaataacg agtataata attacaatt 
      cgtatcaga gaccgaggt atgtccttg aaggtgcaa 
      attcggatt attatcaag ccaagccga cgaggcaaa 
      gatacgaag cggtgccct acagtaacc cgcaggaac 
      attttgtta attctgagg ccgattaca aagggcagt 
      acagcacag tcaacagag ctaatttca ggcccagac 
      tcacgcaag ccctctaca cgagtttct gatttttca 
      agccatatt ctaggtaga cttaaaatt ctatattta 
      atttttttg taaattagt aagcaaatt atggttgtg 
      taaattatg acgattatt taagttaaa ttacatgat 
      actttgtaa aatatttgt actcaaaaa taaaaccgt 
      aaataaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY