Status
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Gene information
General information
TC004584 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7210288..7218182
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004584
      iBeetle screen iB_10606

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004584 RA
        MEVSSQHLL SNEYTEVYR RLPLKLQLA YGVGHVLND 
        VCASMWFTY LLVFFHLVL QFNNWQAGF MLLVGQVAD 
        AVSTPFVGF HSDQSDNFW LCRYGRRKT WHLIGTVCV 
        LVTFPFIFS PCIGCSGSH QWAQIFYYC VFISIFQFG 
        WASVQISHL SLIPELTPN EHDRTKLTA IRYCFTVVS 
        NLLVYVITW GILHISSGE ESKIGPGDA PKFQHVVWT 
        GLSLGILCS VIFHVFVKE EGALGSSDV RGTSLRTPI 
        ADILRSVEV YQVAVVYMS TRLFVNLSQ VFIPLYLHE 
        TLDMAASAL ALIPLIMFI GSFVTSMTI EKLNRCFGR 
        RLSYILGVL MGLAACIWI KWGSGDQFK SSQIHIIAV 
        LIGAGGSIT LVTSLGITA DLIGDKTSS GAFVYGIMS 
        FTDKLANGI AVVIIQDLH KDPSNTGYY RDVLTYVCG 
        GSALLGALA VISFLKKVR GDPLGYENV PNYNSINSD 
        VLPVATEDI QPSLIS
      • >TC004584 RB
        MEVSSQHLL SNEYTEVYR RLPLKLQLA YGVGHVLND 
        VCASMWFTY LLVFFHLVL QFNNWQAGF MLLVGQVAD 
        AVSTPFVGF HSDQSDNFW LCRYGRRKT WHLIGTVCV 
        LVTFPFIFS PCIGCSGSH QWAQIFYYC VFISIFQFG 
        WASVQISHL SLIPELTPN EHDRTKLTA IRYCFTVVS 
        NLLVYVITW GILHISSGE ESKIGPGDA PKFQHVVWT 
        GLSLGILCS VIFHVFVKE EGALGSSDV RGTSLRTPI 
        ADILRSVEV YQVAVVYMS TRLFVNLSQ VFIPLYLHE 
        TLDMAASAL ALIPLIMFI GSFVTSMTI EKLNRCFGR 
        RLSYILGVL MGLAACIWI KWGSGDQFK SSQIHIIAV 
        LIGAGGSIT LVTSLGITA DLIGDKTSS GAFVYGIMS 
        FTDKLANGI AVVIIQDLH KDPSNTGYY RDVLTYVCG 
        GSALLGALA VISFLKKVR GALSRPFRI
      CDS sequence
      • >TC004584 RA
        atggaagtt agcagtcaa cacttgcta agtaacgaa 
        tacacggaa gtttaccgc cgcctgccc ttaaaactg 
        caacttgcc tacggtgtg gggcacgtt ttgaacgat 
        gtgtgcgcg tcgatgtgg ttcacctat ttactcgtg 
        tttttccac ctggttttg cagtttaat aactggcag 
        gcgggtttt atgctcctc gttggccag gtcgcagac 
        gctgtctcc acccccttt gtggggttc cactccgac 
        cagtccgac aatttctgg ctgtgccga tacggaaga 
        cggaaaacg tggcatttg atcggcact gtgtgcgtc 
        ttagtcacc tttcccttc attttttcg ccctgtata 
        ggctgctct gggagccac caatgggcc caaattttt 
        tactactgc gttttcatc tcgattttt cagttcggg 
        tgggcctcc gtgcaaatt tcgcatctc tcgctcata 
        cccgaacta acgccaaat gagcacgat cgcaccaaa 
        ctcaccgcg attagatat tgctttacc gtcgtttcg 
        aatctttta gtatatgtg ataacgtgg ggcattttg 
        cacataagt agcggcgag gagtcgaaa atcgggcct 
        ggggatgcc cccaagttc cagcacgtg gtgtggacg 
        ggcctcagt ctgggtatt ttgtgttcg gtgatattt 
        catgttttt gtgaaagag gagggcgcc cttggaagc 
        agtgatgtc cgagggacg tcgctacga accccaatt 
        gcggatatt ttacgctca gttgaagtg tatcaagtc 
        gcagttgtg tacatgtca actcgactt ttcgtcaat 
        ttgagccaa gtttttatc cctttgtac ctccacgag 
        acgctggat atggcagcg agcgctttg gctctaatc 
        cccttaatt atgtttatc ggaagtttc gtaacttcg 
        atgacaatt gagaaattg aatcgctgt tttggtcgg 
        aggttgtcg tacattttg ggggttttg atggggctt 
        gcggcttgt atttggatc aagtggggt tctggtgat 
        caatttaag tcgtctcag attcacata atcgctgtt 
        ttgattggg gccggtggt tccataact ctagttact 
        agtctaggc ataaccgcg gatcttatt ggtgataag 
        acaagtagt ggtgctttc gtatacgga atcatgagc 
        ttcactgat aagctcgcc aacgggatc gccgtcgtg 
        atcatccaa gatttgcac aaggatccg tcaaatact 
        ggatattac cgagacgtg ctgacatac gtgtgtgga 
        gggtcggcg cttcttgga gctttggct gtgatatct 
        ttccttaag aaagtgaga ggagaccct ttaggatat 
        gaaaacgtc cctaattac aacagtatt aactcggac 
        gtactcccg gtcgccacg gaagatata caaccgtct 
        cttatttca tag
      • >TC004584 RB
        atggaagtt agcagtcaa cacttgcta agtaacgaa 
        tacacggaa gtttaccgc cgcctgccc ttaaaactg 
        caacttgcc tacggtgtg gggcacgtt ttgaacgat 
        gtgtgcgcg tcgatgtgg ttcacctat ttactcgtg 
        tttttccac ctggttttg cagtttaat aactggcag 
        gcgggtttt atgctcctc gttggccag gtcgcagac 
        gctgtctcc acccccttt gtggggttc cactccgac 
        cagtccgac aatttctgg ctgtgccga tacggaaga 
        cggaaaacg tggcatttg atcggcact gtgtgcgtc 
        ttagtcacc tttcccttc attttttcg ccctgtata 
        ggctgctct gggagccac caatgggcc caaattttt 
        tactactgc gttttcatc tcgattttt cagttcggg 
        tgggcctcc gtgcaaatt tcgcatctc tcgctcata 
        cccgaacta acgccaaat gagcacgat cgcaccaaa 
        ctcaccgcg attagatat tgctttacc gtcgtttcg 
        aatctttta gtatatgtg ataacgtgg ggcattttg 
        cacataagt agcggcgag gagtcgaaa atcgggcct 
        ggggatgcc cccaagttc cagcacgtg gtgtggacg 
        ggcctcagt ctgggtatt ttgtgttcg gtgatattt 
        catgttttt gtgaaagag gagggcgcc cttggaagc 
        agtgatgtc cgagggacg tcgctacga accccaatt 
        gcggatatt ttacgctca gttgaagtg tatcaagtc 
        gcagttgtg tacatgtca actcgactt ttcgtcaat 
        ttgagccaa gtttttatc cctttgtac ctccacgag 
        acgctggat atggcagcg agcgctttg gctctaatc 
        cccttaatt atgtttatc ggaagtttc gtaacttcg 
        atgacaatt gagaaattg aatcgctgt tttggtcgg 
        aggttgtcg tacattttg ggggttttg atggggctt 
        gcggcttgt atttggatc aagtggggt tctggtgat 
        caatttaag tcgtctcag attcacata atcgctgtt 
        ttgattggg gccggtggt tccataact ctagttact 
        agtctaggc ataaccgcg gatcttatt ggtgataag 
        acaagtagt ggtgctttc gtatacgga atcatgagc 
        ttcactgat aagctcgcc aacgggatc gccgtcgtg 
        atcatccaa gatttgcac aaggatccg tcaaatact 
        ggatattac cgagacgtg ctgacatac gtgtgtgga 
        gggtcggcg cttcttgga gctttggct gtgatatct 
        ttccttaag aaagtgaga ggagcactt tccagaccc 
        tttaggata tga
      mRNA sequence
      • >TC004584 RA
        ttaatgtga ttcagagcg tttatttct aattttagt 
        agttgtaac aatggaagt tagcagtca acacttgct 
        aagtaacga atacacgga agtttaccg ccgcctgcc 
        cttaaaact gcaacttgc ctacggtgt ggggcacgt 
        tttgaacga tgtgtgcgc gtcgatgtg gttcaccta 
        tttactcgt gtttttcca cctggtttt gcagtttaa 
        taactggca ggcgggttt tatgctcct cgttggcca 
        ggtcgcaga cgctgtctc caccccctt tgtggggtt 
        ccactccga ccagtccga caatttctg gctgtgccg 
        atacggaag acggaaaac gtggcattt gatcggcac 
        tgtgtgcgt cttagtcac ctttccctt cattttttc 
        gccctgtat aggctgctc tgggagcca ccaatgggc 
        ccaaatttt ttactactg cgttttcat ctcgatttt 
        tcagttcgg gtgggcctc cgtgcaaat ttcgcatct 
        ctcgctcat acccgaact aacgccaaa tgagcacga 
        tcgcaccaa actcaccgc gattagata ttgctttac 
        cgtcgtttc gaatctttt agtatatgt gataacgtg 
        gggcatttt gcacataag tagcggcga ggagtcgaa 
        aatcgggcc tggggatgc ccccaagtt ccagcacgt 
        ggtgtggac gggcctcag tctgggtat tttgtgttc 
        ggtgatatt tcatgtttt tgtgaaaga ggagggcgc 
        ccttggaag cagtgatgt ccgagggac gtcgctacg 
        aaccccaat tgcggatat tttacgctc agttgaagt 
        gtatcaagt cgcagttgt gtacatgtc aactcgact 
        tttcgtcaa tttgagcca agtttttat ccctttgta 
        cctccacga gacgctgga tatggcagc gagcgcttt 
        ggctctaat ccccttaat tatgtttat cggaagttt 
        cgtaacttc gatgacaat tgagaaatt gaatcgctg 
        ttttggtcg gaggttgtc gtacatttt gggggtttt 
        gatggggct tgcggcttg tatttggat caagtgggg 
        ttctggtga tcaatttaa gtcgtctca gattcacat 
        aatcgctgt tttgattgg ggccggtgg ttccataac 
        tctagttac tagtctagg cataaccgc ggatcttat 
        tggtgataa gacaagtag tggtgcttt cgtatacgg 
        aatcatgag cttcactga taagctcgc caacgggat 
        cgccgtcgt gatcatcca agatttgca caaggatcc 
        gtcaaatac tggatatta ccgagacgt gctgacata 
        cgtgtgtgg agggtcggc gcttcttgg agctttggc 
        tgtgatatc tttccttaa gaaagtgag aggagaccc 
        tttaggata tgaaaacgt ccctaatta caacagtat 
        taactcgga cgtactccc ggtcgccac ggaagatat 
        acaaccgtc tcttatttc atagtgcaa tttttattt 
        attaacaga gtggatata tttttataa aactgtatc 
        gtattacta gttgttatt aattaataa aaaatatga 
        agttat
      • >TC004584 RB
        ttaatgtga ttcagagcg tttatttct aattttagt 
        agttgtaac aatggaagt tagcagtca acacttgct 
        aagtaacga atacacgga agtttaccg ccgcctgcc 
        cttaaaact gcaacttgc ctacggtgt ggggcacgt 
        tttgaacga tgtgtgcgc gtcgatgtg gttcaccta 
        tttactcgt gtttttcca cctggtttt gcagtttaa 
        taactggca ggcgggttt tatgctcct cgttggcca 
        ggtcgcaga cgctgtctc caccccctt tgtggggtt 
        ccactccga ccagtccga caatttctg gctgtgccg 
        atacggaag acggaaaac gtggcattt gatcggcac 
        tgtgtgcgt cttagtcac ctttccctt cattttttc 
        gccctgtat aggctgctc tgggagcca ccaatgggc 
        ccaaatttt ttactactg cgttttcat ctcgatttt 
        tcagttcgg gtgggcctc cgtgcaaat ttcgcatct 
        ctcgctcat acccgaact aacgccaaa tgagcacga 
        tcgcaccaa actcaccgc gattagata ttgctttac 
        cgtcgtttc gaatctttt agtatatgt gataacgtg 
        gggcatttt gcacataag tagcggcga ggagtcgaa 
        aatcgggcc tggggatgc ccccaagtt ccagcacgt 
        ggtgtggac gggcctcag tctgggtat tttgtgttc 
        ggtgatatt tcatgtttt tgtgaaaga ggagggcgc 
        ccttggaag cagtgatgt ccgagggac gtcgctacg 
        aaccccaat tgcggatat tttacgctc agttgaagt 
        gtatcaagt cgcagttgt gtacatgtc aactcgact 
        tttcgtcaa tttgagcca agtttttat ccctttgta 
        cctccacga gacgctgga tatggcagc gagcgcttt 
        ggctctaat ccccttaat tatgtttat cggaagttt 
        cgtaacttc gatgacaat tgagaaatt gaatcgctg 
        ttttggtcg gaggttgtc gtacatttt gggggtttt 
        gatggggct tgcggcttg tatttggat caagtgggg 
        ttctggtga tcaatttaa gtcgtctca gattcacat 
        aatcgctgt tttgattgg ggccggtgg ttccataac 
        tctagttac tagtctagg cataaccgc ggatcttat 
        tggtgataa gacaagtag tggtgcttt cgtatacgg 
        aatcatgag cttcactga taagctcgc caacgggat 
        cgccgtcgt gatcatcca agatttgca caaggatcc 
        gtcaaatac tggatatta ccgagacgt gctgacata 
        cgtgtgtgg agggtcggc gcttcttgg agctttggc 
        tgtgatatc tttccttaa gaaagtgag aggagcact 
        ttccagacc ctttaggat atgaaaacg tccctaatt 
        acaacagta ttaactcgg acgtactcc cggtcgcca 
        cggaagata tacaaccgt ctcttattt catagtgca 
        atttttatt tattaacag agtggatat atttttata 
        aaactgtat cgtattact agttgttat taattaata 
        aaaaatatg aagttat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY