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Gene information
General information
TC009787 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8131932..8133820
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009787
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10605

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009787 RA
      MGAKNLLLG HLKVILGNH VESDIVDYI HSIKNKEDL 
      DEFMENIID KNNTGHVTS YEGICNILF GSNKASKKS 
      HSEAMVASK SQEKNNKAM KQKGKKRFS DINAFQEKK 
      KQQEGRTMC DCEGQEHEF MNNCLNCGR IHCYEEGPG 
      PCFFCNEPV TLMGEENYV DLSSSRKSR KSASVTKHK 
      ENTVFDDDN DYFKTDSNQ NKQDKRTLV VALDFATRR 
      VIESTAEDV RIKEKLHED IKKHLANVE KLYRKLQKE 
      SEVTIAPDN NDLVDLLVQ MRHKKPVGV EDLGPEDNK 
      AHIIDYGKV YDEEVIANT DHGLCLSMH QPYASLLVA 
      GVKKHEGRT WKTNHRGRL WIAAAAKVP DEDEISALE 
      TFYRHYYND QSLKFPRDY PTSCLLGCV FVEDCLDQE 
      TYRKRFPNG ESNSPYVLI CSNPVILPI FYPILGKHK 
      IYPLDKELH NCAKLSLRH ANYFD
    CDS sequence
    • >TC009787 RA
      atgggtgcc aagaatttg ctgttgggc cacttgaag 
      gtgattttg ggaaatcat gtcgaatcg gatattgtc 
      gattacata catagcata aaaaacaag gaagattta 
      gatgaattt atggaaaat ataattgat aagaacaat 
      accggtcat gttacatcg tacgagggg atttgtaat 
      attttattt ggtagtaat aaggcctca aaaaaaagt 
      cattctgag gctatggtg gcttccaaa agtcaggaa 
      aaaaataat aaggcaatg aagcaaaaa ggcaagaaa 
      cgcttcagt gatataaac gcattccaa gagaagaaa 
      aaacaacag gaaggtagg acaatgtgt gactgtgaa 
      ggtcaggag cacgaattt atgaataat tgtttgaat 
      tgtggacga attcattgt tatgaggaa ggcccaggg 
      ccttgcttt ttctgcaat gaaccagtt actttaatg 
      ggcgaagag aattacgta gatttatca tcttcacgc 
      aaaagcagg aaaagtgct tctgtcaca aaacataaa 
      gaaaacaca gtttttgat gacgataac gactacttt 
      aaaaccgac tcaaatcag aataaacaa gacaaacgc 
      accttggtt gtagctctt gactttgcg acccgtcga 
      gtaattgaa agcacggct gaagatgtg cgaattaag 
      gagaaactt cacgaagac attaaaaaa catctcgct 
      aatgtcgag aaattgtac cggaaattg caaaaagaa 
      tcggaagtt actatagcg cctgataat aacgatttg 
      gtcgatttg ttggtacaa atgagacac aaaaagccg 
      gtcggagtt gaagattta ggtccggaa gataacaaa 
      gcccatatt atcgattat gggaaggtt tatgacgag 
      gaggttatc gcaaatacc gaccatggg ctgtgcctg 
      agtatgcac caaccgtat gctagtctt ctggtagcg 
      ggagtcaaa aaacacgaa ggcaggacg tggaaaacc 
      aatcacagg gggcgcctt tggatcgca gcggctgct 
      aaagtacca gacgaggat gaaatttcc gcgttagaa 
      acattttat cgccactac tacaacgac cagtcatta 
      aaatttcca cgagactat ccaacgagt tgcctccta 
      ggctgtgtt ttcgtggaa gattgttta gaccaagaa 
      acttaccga aaacgtttc ccgaacggc gagagtaac 
      agtccatac gtcttaatt tgctcaaat cccgtcatt 
      ttgcctatt ttttaccca atcctcggc aaacataaa 
      atatatccg ttagataag gagttgcac aattgcgcc 
      aaattatca ctccgacat gcgaactac ttcgattga
    mRNA sequence
    • >TC009787 RA
      gggtaaggt tagacaagt gttttttgg gaagttttt 
      ggaaaattt ggcataaat gggtgccaa gaatttgct 
      gttgggcca cttgaaggt gattttggg aaatcatgt 
      cgaatcgga tattgtcga ttacataca tagcataaa 
      aaacaagga agatttaga tgaatttat ggaaaatat 
      aattgataa gaacaatac cggtcatgt tacatcgta 
      cgaggggat ttgtaatat tttatttgg tagtaataa 
      ggcctcaaa aaaaagtca ttctgaggc tatggtggc 
      ttccaaaag tcaggaaaa aaataataa ggcaatgaa 
      gcaaaaagg caagaaacg cttcagtga tataaacgc 
      attccaaga gaagaaaaa acaacagga aggtaggac 
      aatgtgtga ctgtgaagg tcaggagca cgaatttat 
      gaataattg tttgaattg tggacgaat tcattgtta 
      tgaggaagg cccagggcc ttgcttttt ctgcaatga 
      accagttac tttaatggg cgaagagaa ttacgtaga 
      tttatcatc ttcacgcaa aagcaggaa aagtgcttc 
      tgtcacaaa acataaaga aaacacagt ttttgatga 
      cgataacga ctactttaa aaccgactc aaatcagaa 
      taaacaaga caaacgcac cttggttgt agctcttga 
      ctttgcgac ccgtcgagt aattgaaag cacggctga 
      agatgtgcg aattaagga gaaacttca cgaagacat 
      taaaaaaca tctcgctaa tgtcgagaa attgtaccg 
      gaaattgca aaaagaatc ggaagttac tatagcgcc 
      tgataataa cgatttggt cgatttgtt ggtacaaat 
      gagacacaa aaagccggt cggagttga agatttagg 
      tccggaaga taacaaagc ccatattat cgattatgg 
      gaaggttta tgacgagga ggttatcgc aaataccga 
      ccatgggct gtgcctgag tatgcacca accgtatgc 
      tagtcttct ggtagcggg agtcaaaaa acacgaagg 
      caggacgtg gaaaaccaa tcacagggg gcgcctttg 
      gatcgcagc ggctgctaa agtaccaga cgaggatga 
      aatttccgc gttagaaac attttatcg ccactacta 
      caacgacca gtcattaaa atttccacg agactatcc 
      aacgagttg cctcctagg ctgtgtttt cgtggaaga 
      ttgtttaga ccaagaaac ttaccgaaa acgtttccc 
      gaacggcga gagtaacag tccatacgt cttaatttg 
      ctcaaatcc cgtcatttt gcctatttt ttacccaat 
      cctcggcaa acataaaat atatccgtt agataagga 
      gttgcacaa ttgcgccaa attatcact ccgacatgc 
      gaactactt cgattgaaa aaacaaata gattcttat 
      gtttattgc actttttgt ttgtttaca aaaatataa 
      taaattaca actatacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY