Status
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Gene information
General information
TC030930
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005278
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450498.1: 50899..54243
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC030930
      iBeetle screen iB_10601

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC030930 RA
        MANKQIRFM QLNMNRCAA AHDLANKIM QEERIDILL 
        GQEPNSKKA QRLICDTNC GSFIVMGNE VSYNNLICD 
        KDYLAADLP SLRIVSTYF SPNGSEEDF ILLLESLEQ 
        RVREGNLET IISGDLNAK SPIFGSSVT NRRGILLEE 
        FVESNNLIV LNKGDEATF IGPGGSSVI DVTLATTKV 
        GGRIRDWRV DTQENLSDH QTIKFSICA EFSMPKTLY 
        ETGGWKITP KSWATVEAM ATLTPMLKK ETCTPTEMI 
        QQITEFCNK YLPMRQKTS RRRSVFWWT KEISETRIC 
        CMKLRRRYL RLRKKQQHT EEDYKEYIG AKRALKTQI 
        KKQKDKCWK ELCDKLEGD IWGKAYKIV MGRLGPRPQ 
        TTLKDEQIV SQVQKLFPN HPTRRWSID GTEDVPVPI 
        STQEILEAA KCIKTKKAP GLDYLLPEV VKLAAKTCP 
        NEVAVVFNS CLNRGEFPE IWKSARLVL IEKPKKCPS 
        DSPTYRPIC LLNVSGKLL ETVIKRRLE KELRERGIP 
        HEHQYGFRS GKSTMDAIT KVLEAPNSI EEKISYKRK 
        KFCLLITLD IENAFNSAP WDGIIAALE RSGVSRYLN 
        NIIKSYLTD RYIETAERK KIRMTCGVP QGSVLGPTL 
        WNLFYDQIL RLDMEAGVY TVAYADDLA VVVTDVCVD 
        RLRDKAEYA IRNIIDKLQ NMGIRLCLH KTEMILMSG 
        RRKLRELTV RIGELEITT KPNLNYMGI VLDITADKV 
        MGKLSRVLP NIGGPSLLK RKVLTSVVT SIIYYAVPT 
        WSKVLKYNA YLAKLERIT RRMALRITM AYRTSPTKA 
        LLVIAGVPP VQLKVQERC KIYQKNEKE QAQKELLQK 
        WQESWNTYE GWTKVFIKN IELWINKKV VGVDHHVTQ 
        ALTGHGAFG RYLQRIKKQ DHARCQYCD WETDDARHT 
        IFQCVRWEN VRLDAETRC GRIINEETI STLLTGEED 
        TSDAVFNMI RSIMREKNK DGVKINK
      CDS sequence
      • >TC030930 RA
        atggctaac aaacaaata agatttatg cagcttaat 
        atgaatcgg tgcgcagct gcccacgac ttggctaac 
        aaaataatg caagaagaa agaatagac atccttctt 
        ggacaggag cctaatagt aaaaaagct caaagactt 
        atctgtgat acgaactgt ggtagtttt attgtgatg 
        ggaaatgaa gtgtcttat aataacctg atctgtgat 
        aaagattat ctagcggcc gatctacca tcgctacga 
        attgtgtct acctacttt tctcctaat gggagcgag 
        gaagacttt attctgctt ctggaaagc ttggagcag 
        cgagtaagg gaaggtaat ctagaaacg attatctca 
        ggggatcta aacgcaaaa tcgccgatc ttcggatcg 
        tctgttact aaccgtagg ggcatcctt ctagaggag 
        tttgtagag tctaacaac cttatagta ctaaataaa 
        ggagacgag gctacgttt attgggccc ggtggaagc 
        tccgttatc gatgtgacg cttgcgact acaaaagtt 
        gggggaagg atacgggac tggagggtt gatactcag 
        gaaaacctg tccgaccac caaactatc aaattttca 
        atatgtgct gaattttcc atgccaaaa acattatat 
        gaaaccggt ggatggaag ataacacct aaatcctgg 
        gccacagta gaggcaatg gcaacctta actccaatg 
        ctcaaaaaa gaaacttgt acaccaacc gaaatgatc 
        cagcaaatt actgagttt tgtaataaa tatttacct 
        atgcgacag aaaacgtca aggcgccgt tcagttttc 
        tggtggacg aaagagatt tctgaaaca agaatctgc 
        tgtatgaaa cttagaaga agatatctt cgtctaaga 
        aaaaagcaa caacacact gaagaagat tataaagaa 
        tacataggg gccaaaaga gctttaaaa actcaaata 
        aagaaacaa aaagacaaa tgctggaag gagctttgt 
        gacaagctg gaaggtgat atctggggt aaggcatac 
        aaaatagtt atgggcaga ctaggacct aggcctcaa 
        acaacgcta aaagatgag caaattgta agtcaagta 
        caaaaactt tttcccaat cacccaact aggcggtgg 
        tcaatagat ggtactgaa gatgtgcca gtcccaata 
        agtacgcag gaaatccta gaagcagct aaatgtatt 
        aaaacaaaa aaagcacca gggttggat tacttactt 
        cctgaagta gtcaagtta gctgctaaa acatgccca 
        aatgaggtc gcagttgtg tttaattcc tgcctaaat 
        cgtggcgaa tttccggaa atctggaag tcagcaagg 
        ctggtactt atagagaag cccaagaag tgtccaagc 
        gatagtcct acatacaga ccaatatgt cttctaaac 
        gtgtccggt aaactctta gaaacagtt atcaaacgc 
        aggctggaa aaagaactg cgagaacgc ggtattccg 
        catgaacat caatatggc ttcagaagc ggcaagtcg 
        acaatggat gcaatcacc aaggtttta gaggcacct 
        aatagcata gaggaaaaa atatcttat aagaggaaa 
        aaattttgc ttacttata acgttggac attgaaaac 
        gcgtttaac tcagctccg tgggatggt ataatagcg 
        gccctggaa cgcagcggt gtaagtaga taccttaat 
        aacatcata aaatcctac ctaacagac aggtatata 
        gaaaccgca gaaagaaaa aagatacgt atgacctgt 
        ggtgttcca cagggctcg gtcctcgga cctaccttg 
        tggaacctc ttctacgat caaattcta cgcctggac 
        atggaagct ggagtttat accgttgca tatgccgac 
        gatcttgct gtcgtagtg acagatgta tgtgtggat 
        agactaaga gataaagcg gaatatgca attcgaaat 
        ataatagat aagctacag aatatgggt attagactg 
        tgcttacat aagaccgaa atgatcctg atgagtgga 
        cgacgaaaa ctacgagaa ctgacggtt agaattggg 
        gagctggaa atcacaaca aaacctaat cttaactat 
        atgggcatt gtattggat attacagcg gacaaagtt 
        atgggaaaa ctgtctcga gtgcttccg aacatagga 
        ggcccatca ttgttaaaa agaaaagtg ctgacttca 
        gtggtaacc tcaataatc tattacgca gttcctact 
        tggtcaaaa gtgcttaaa tataatgct tatctagct 
        aaactagaa agaattacc agacgcatg gcccttaga 
        attacaatg gcttatagg acttcacca actaaggct 
        ctactggtt attgcagga gtgccgccg gttcaatta 
        aaggtgcaa gaacgctgc aaaatatac caaaaaaat 
        gaaaaagag caggctcaa aaagaactt ttacaaaaa 
        tggcaggaa agttggaat acatacgaa ggatggaca 
        aaagttttc ataaaaaat atagagctg tggattaat 
        aagaaggta gtaggcgtt gaccaccac gtgacacag 
        gcactcacg ggccatggg gccttcggg aggtatcta 
        caaagaata aaaaaacag gaccatgct aggtgccag 
        tattgcgac tgggaaacc gatgacgca agacacacg 
        atcttccaa tgcgttcgt tgggaaaat gttaggcta 
        gacgccgaa acacgctgc ggtagaata ataaacgag 
        gaaacaata agcacttta ttaactgga gaagaagac 
        acaagtgac gctgtattc aatatgatt agaagtatt 
        atgagagag aaaaacaaa gatggagta aaaataaac 
        aaatag
      mRNA sequence
      • >TC030930 RA
        aggaccttg gcagtaaca gctacagta gaccagaga 
        acatcagat aaaataata gaatcgcgt tacctgcgg 
        gttggttta gtaaagtgc agcgtagaa aaacggata 
        gacttgaaa aggtgtttt aaatgttgg gcttttgac 
        catgaggca gctcaatgc gatggaaca gatagaaag 
        gatttgtgc tttaaatgc ggacaaaaa ggtcacgca 
        tccaaaacc tgtgaaaat gaagaatat tgccctatt 
        tgtgctgtt caaggacat aaaaccgga ggtggccgc 
        tgcaaagct ttcaagaag gcacttagt ttagcaagg 
        aggggcgaa agacaacaa ttattctaa atggctaac 
        aaacaaata agatttatg cagcttaat atgaatcgg 
        tgcgcagct gcccacgac ttggctaac aaaataatg 
        caagaagaa agaatagac atccttctt ggacaggag 
        cctaatagt aaaaaagct caaagactt atctgtgat 
        acgaactgt ggtagtttt attgtgatg ggaaatgaa 
        gtgtcttat aataacctg atctgtgat aaagattat 
        ctagcggcc gatctacca tcgctacga attgtgtct 
        acctacttt tctcctaat gggagcgag gaagacttt 
        attctgctt ctggaaagc ttggagcag cgagtaagg 
        gaaggtaat ctagaaacg attatctca ggggatcta 
        aacgcaaaa tcgccgatc ttcggatcg tctgttact 
        aaccgtagg ggcatcctt ctagaggag tttgtagag 
        tctaacaac cttatagta ctaaataaa ggagacgag 
        gctacgttt attgggccc ggtggaagc tccgttatc 
        gatgtgacg cttgcgact acaaaagtt gggggaagg 
        atacgggac tggagggtt gatactcag gaaaacctg 
        tccgaccac caaactatc aaattttca atatgtgct 
        gaattttcc atgccaaaa acattatat gaaaccggt 
        ggatggaag ataacacct aaatcctgg gccacagta 
        gaggcaatg gcaacctta actccaatg ctcaaaaaa 
        gaaacttgt acaccaacc gaaatgatc cagcaaatt 
        actgagttt tgtaataaa tatttacct atgcgacag 
        aaaacgtca aggcgccgt tcagttttc tggtggacg 
        aaagagatt tctgaaaca agaatctgc tgtatgaaa 
        cttagaaga agatatctt cgtctaaga aaaaagcaa 
        caacacact gaagaagat tataaagaa tacataggg 
        gccaaaaga gctttaaaa actcaaata aagaaacaa 
        aaagacaaa tgctggaag gagctttgt gacaagctg 
        gaaggtgat atctggggt aaggcatac aaaatagtt 
        atgggcaga ctaggacct aggcctcaa acaacgcta 
        aaagatgag caaattgta agtcaagta caaaaactt 
        tttcccaat cacccaact aggcggtgg tcaatagat 
        ggtactgaa gatgtgcca gtcccaata agtacgcag 
        gaaatccta gaagcagct aaatgtatt aaaacaaaa 
        aaagcacca gggttggat tacttactt cctgaagta 
        gtcaagtta gctgctaaa acatgccca aatgaggtc 
        gcagttgtg tttaattcc tgcctaaat cgtggcgaa 
        tttccggaa atctggaag tcagcaagg ctggtactt 
        atagagaag cccaagaag tgtccaagc gatagtcct 
        acatacaga ccaatatgt cttctaaac gtgtccggt 
        aaactctta gaaacagtt atcaaacgc aggctggaa 
        aaagaactg cgagaacgc ggtattccg catgaacat 
        caatatggc ttcagaagc ggcaagtcg acaatggat 
        gcaatcacc aaggtttta gaggcacct aatagcata 
        gaggaaaaa atatcttat aagaggaaa aaattttgc 
        ttacttata acgttggac attgaaaac gcgtttaac 
        tcagctccg tgggatggt ataatagcg gccctggaa 
        cgcagcggt gtaagtaga taccttaat aacatcata 
        aaatcctac ctaacagac aggtatata gaaaccgca 
        gaaagaaaa aagatacgt atgacctgt ggtgttcca 
        cagggctcg gtcctcgga cctaccttg tggaacctc 
        ttctacgat caaattcta cgcctggac atggaagct 
        ggagtttat accgttgca tatgccgac gatcttgct 
        gtcgtagtg acagatgta tgtgtggat agactaaga 
        gataaagcg gaatatgca attcgaaat ataatagat 
        aagctacag aatatgggt attagactg tgcttacat 
        aagaccgaa atgatcctg atgagtgga cgacgaaaa 
        ctacgagaa ctgacggtt agaattggg gagctggaa 
        atcacaaca aaacctaat cttaactat atgggcatt 
        gtattggat attacagcg gacaaagtt atgggaaaa 
        ctgtctcga gtgcttccg aacatagga ggcccatca 
        ttgttaaaa agaaaagtg ctgacttca gtggtaacc 
        tcaataatc tattacgca gttcctact tggtcaaaa 
        gtgcttaaa tataatgct tatctagct aaactagaa 
        agaattacc agacgcatg gcccttaga attacaatg 
        gcttatagg acttcacca actaaggct ctactggtt 
        attgcagga gtgccgccg gttcaatta aaggtgcaa 
        gaacgctgc aaaatatac caaaaaaat gaaaaagag 
        caggctcaa aaagaactt ttacaaaaa tggcaggaa 
        agttggaat acatacgaa ggatggaca aaagttttc 
        ataaaaaat atagagctg tggattaat aagaaggta 
        gtaggcgtt gaccaccac gtgacacag gcactcacg 
        ggccatggg gccttcggg aggtatcta caaagaata 
        aaaaaacag gaccatgct aggtgccag tattgcgac 
        tgggaaacc gatgacgca agacacacg atcttccaa 
        tgcgttcgt tgggaaaat gttaggcta gacgccgaa 
        acacgctgc ggtagaata ataaacgag gaaacaata 
        agcacttta ttaactgga gaagaagac acaagtgac 
        gctgtattc aatatgatt agaagtatt atgagagag 
        aaaaacaaa gatggagta aaaataaac aaatagaat 
        agaagacta ggcgaaggc taaagtaga cactatctg 
        ggaagaaat gccgaaagg cgat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY