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Gene information
General information
TC008732 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15148917..15168441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008732
    iBeetle screen iB_10598

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008732 RA
      MYLKVCKTN LLQSARKQF RNYHSDNVF GFKRKSKHV 
      YTAPEQVLK AQCDQSNFF RLVSAFRQN AHKTADVNP 
      VALTREVTI FPDFDPKRY CLDPDTKIK FTGILNTTQ 
      SEGTVAEAV DFLRHVYGK NIAAEFTHL ENPEEIDWF 
      CNRLEQLVP EEIDTETKL RLATELLRS QNFDNFLAN 
      KFTGVKRYG GEGAESMMA FFSEFFRLA AGDSVEQLV 
      VAMPHRGRL NLLTGMLNF PPVKMFAKL KGKSNFSDK 
      YRVTGDVLS HFVSSTDLK FGDKNLHVT LLYNPSHLE 
      AVNPVSMGK TRAKQLESR DGDYGDGRW GDKIINLQV 
      HGDAAITGQ GVNQECLAL SGTPHFEIG GSIHLVVNN 
      QLGFTTPAD RGRSSRYCT DLAKMISAP VIHVNGDFP 
      EMVLKATKL AFEYQRKFR KEVFIDMNC YRQWGHNEL 
      DDPTFTNPS LYGIIRSRG TVPDQYTKK LIDEGILNE 
      EEKNTIIKE HTKWLNEHL KAVDSYKPD DVYFKKQWE 
      GFSQADEAI TTWDTGIHL DLLTYIGSK SVQFPEHFN 
      VHPTLLKTH VKNRLARVA EGVNIDWST AETLAFGSL 
      LYEGYNIRI SGQDVGRGT FSHRHVMLV DQQTNHIYI 
      PLNNLHENQ GGFLEIANS ILSEEAVLG YEYGMSIES 
      PKNLIIWEA QFGDFFNGA QIIFDTFVS NGEVKWLWS 
      SGLVVLLPH GYDGAGPEH SSARIERFL QMTDSKEDK 
      VDGDNVNMQ VCHPSTPAQ YFHLLRRQM LRNFRKPLI 
      VIGPKTLLR LPEATSNFA DMGPQTTFQ PVITDNTHD 
      PKQIQVVLL TSGKHFYNL ASKRESLGA KNVAIVRLE 
      SYCPFPTLE LQQEVAKFP NAKIFMWCQ EEPQNMGAW 
      SFVKPRFEN LVGRKIQFC GRPTQAAPA VGIGELHDQ 
      QVSEILSKP FSFEI
    CDS sequence
    • >TC008732 RA
      atgtactta aaagtgtgc aaaacaaac ctactccaa 
      agtgcccgt aaacaattt cgaaattac catagtgat 
      aatgttttc ggttttaag cggaaaagt aaacatgtt 
      tacaccgca cccgaacaa gtattaaaa gcccaatgc 
      gaccaaagc aacttcttc cgtctcgtc agcgctttc 
      cgacaaaat gcccacaaa accgccgac gtaaatccg 
      gtcgctctc acccgcgaa gtaacaatt ttcccggat 
      tttgacccg aaacgctac tgcctcgac cccgacacc 
      aaaatcaaa ttcaccgga attttaaac acgacgcag 
      tccgaaggc acagttgcc gaagccgtg gacttcctc 
      cgacatgtc tacgggaag aatatcgcc gccgaattc 
      acccattta gagaacccg gaagagatc gactggttt 
      tgtaaccgg ctcgaacaa ctcgtcccg gaagaaatc 
      gacacggag acaaaactc cggctagcg acagagttg 
      ctccggtcg cagaacttc gacaatttt ctcgctaat 
      aaattcact ggtgttaag cgatacggg ggcgaaggc 
      gccgaaagc atgatggcg ttttttagc gagtttttt 
      cgccttgcg gcaggagat agcgtcgaa cagttggtg 
      gttgcaatg ccgcatagg ggacgcttg aatcttctc 
      accggaatg ctcaatttt cctccggtg aagatgttt 
      gccaagctg aaggggaag agcaacttt tcggacaag 
      tatcgtgtg acgggggat gttttaagt cattttgtg 
      tcttcaact gatttgaaa tttggagat aaaaatctt 
      cacgtaact ctgttgtac aatccttcg catttggaa 
      gctgttaat cccgtctca atggggaag actagagcc 
      aaacaattg gaaagtcgc gatggggac tatggggat 
      gggagatgg ggtgataaa attattaat ttacaagtg 
      catggagac gctgcgatt actggacaa ggagttaat 
      caggaatgt ttagcttta agtggaact ccacatttt 
      gaaattggt ggctctatc cacttagtt gttaataat 
      cagttaggt tttactaca ccagcagat cggggaaga 
      tcatcaagg tattgcacc gacttagct aaaatgatt 
      tcagcaccg gttattcac gtcaatgga gacttcccc 
      gagatggtt ctaaaagct acaaaatta gcatttgaa 
      tatcaacgt aaattccga aaagaagtt tttatcgat 
      atgaattgc tacagacaa tggggacac aacgaatta 
      gacgatccg acttttact aatccttca ctttacgga 
      attatcaga tctagaggt acagtccct gaccaatat 
      acgaaaaaa ctaatcgac gaagggatt ttaaacgag 
      gaagaaaaa aataccatc atcaaagaa catacgaaa 
      tggcttaat gagcacttg aaagctgta gattcttac 
      aaacctgac gacgtttat ttcaaaaaa caatgggag 
      gggttttcg caagctgac gaagccata acgacttgg 
      gacaccgga attcatcta gatcttctc acatacatc 
      ggaagcaaa tcggtccaa tttccggaa catttcaat 
      gtccatccg actctcctc aaaacccac gtaaaaaat 
      cgcctggct cgagtggcc gaaggcgta aatatcgac 
      tggtcaacg gctgaaact ctcgctttt ggttcatta 
      ttatacgag gggtataat atacgcatt agcggccag 
      gatgtggga aggggcact ttttcgcac cgccacgta 
      atgctagtc gatcaacaa acaaaccat atttatatt 
      cccttgaac aacttgcac gaaaaccaa ggcggtttc 
      ctcgaaatt gctaatagt attttatca gaagaggcg 
      gttctaggg tacgagtac ggcatgagc atcgaaagt 
      ccgaaaaat ctaattatt tgggaagcc cagtttggg 
      gattttttc aacggcgcc caaattatc ttcgacacg 
      tttgtatca aacggagaa gtgaagtgg ttatggagt 
      agcggttta gttgtgctt ctaccacat gggtacgat 
      ggagccggt cctgaacac agctcagcc agaatcgaa 
      aggtttcta caaatgacc gattccaaa gaagataaa 
      gtcgacggt gataatgtt aatatgcaa gtttgtcat 
      ccgagcacc ccagcacaa tatttccat ttactacga 
      agacagatg ttgcgcaat tttagaaaa cctttaatt 
      gtaataggc ccgaaaaca cttttaaga ctgccggaa 
      gctacctca aatttcgcc gacatgggt cctcaaaca 
      acatttcaa cccgtcata accgataat actcacgac 
      ccgaaacaa atccaagtc gttctgtta acctcagga 
      aaacacttt tacaatctt gcttcaaaa agggaatca 
      ctaggtgcc aaaaatgtg gcaattgtt cgattagaa 
      tcgtattgc ccgtttccg actctagaa ttacaacag 
      gaagttgcg aaatttccc aatgcaaaa atttttatg 
      tggtgtcaa gaagagcca caaaacatg ggcgcttgg 
      agttttgtg aaacctcga tttgagaat ttagttggc 
      agaaaaatt cagttttgt ggtcgacca actcaagct 
      gctccagcg gttggaatt ggcgaattg cacgaccag 
      caagtgtcg gaaatcctg agcaaaccg ttttcgttt 
      gaaatttaa
    mRNA sequence
    • >TC008732 RA
      gtcagtagc aagttcaaa tatgattta gtcaagatt 
      tagcacgct tgtgataat tttccattt ctaaaggta 
      ttagaaaat gtacttaaa agtgtgcaa aacaaacct 
      actccaaag tgcccgtaa acaatttcg aaattacca 
      tagtgataa tgttttcgg ttttaagcg gaaaagtaa 
      acatgttta caccgcacc cgaacaagt attaaaagc 
      ccaatgcga ccaaagcaa cttcttccg tctcgtcag 
      cgctttccg acaaaatgc ccacaaaac cgccgacgt 
      aaatccggt cgctctcac ccgcgaagt aacaatttt 
      cccggattt tgacccgaa acgctactg cctcgaccc 
      cgacaccaa aatcaaatt caccggaat tttaaacac 
      gacgcagtc cgaaggcac agttgccga agccgtgga 
      cttcctccg acatgtcta cgggaagaa tatcgccgc 
      cgaattcac ccatttaga gaacccgga agagatcga 
      ctggttttg taaccggct cgaacaact cgtcccgga 
      agaaatcga cacggagac aaaactccg gctagcgac 
      agagttgct ccggtcgca gaacttcga caattttct 
      cgctaataa attcactgg tgttaagcg atacggggg 
      cgaaggcgc cgaaagcat gatggcgtt ttttagcga 
      gttttttcg ccttgcggc aggagatag cgtcgaaca 
      gttggtggt tgcaatgcc gcatagggg acgcttgaa 
      tcttctcac cggaatgct caattttcc tccggtgaa 
      gatgtttgc caagctgaa ggggaagag caacttttc 
      ggacaagta tcgtgtgac gggggatgt tttaagtca 
      ttttgtgtc ttcaactga tttgaaatt tggagataa 
      aaatcttca cgtaactct gttgtacaa tccttcgca 
      tttggaagc tgttaatcc cgtctcaat ggggaagac 
      tagagccaa acaattgga aagtcgcga tggggacta 
      tggggatgg gagatgggg tgataaaat tattaattt 
      acaagtgca tggagacgc tgcgattac tggacaagg 
      agttaatca ggaatgttt agctttaag tggaactcc 
      acattttga aattggtgg ctctatcca cttagttgt 
      taataatca gttaggttt tactacacc agcagatcg 
      gggaagatc atcaaggta ttgcaccga cttagctaa 
      aatgatttc agcaccggt tattcacgt caatggaga 
      cttccccga gatggttct aaaagctac aaaattagc 
      atttgaata tcaacgtaa attccgaaa agaagtttt 
      tatcgatat gaattgcta cagacaatg gggacacaa 
      cgaattaga cgatccgac ttttactaa tccttcact 
      ttacggaat tatcagatc tagaggtac agtccctga 
      ccaatatac gaaaaaact aatcgacga agggatttt 
      aaacgagga agaaaaaaa taccatcat caaagaaca 
      tacgaaatg gcttaatga gcacttgaa agctgtaga 
      ttcttacaa acctgacga cgtttattt caaaaaaca 
      atgggaggg gttttcgca agctgacga agccataac 
      gacttggga caccggaat tcatctaga tcttctcac 
      atacatcgg aagcaaatc ggtccaatt tccggaaca 
      tttcaatgt ccatccgac tctcctcaa aacccacgt 
      aaaaaatcg cctggctcg agtggccga aggcgtaaa 
      tatcgactg gtcaacggc tgaaactct cgcttttgg 
      ttcattatt atacgaggg gtataatat acgcattag 
      cggccagga tgtgggaag gggcacttt ttcgcaccg 
      ccacgtaat gctagtcga tcaacaaac aaaccatat 
      ttatattcc cttgaacaa cttgcacga aaaccaagg 
      cggtttcct cgaaattgc taatagtat tttatcaga 
      agaggcggt tctagggta cgagtacgg catgagcat 
      cgaaagtcc gaaaaatct aattatttg ggaagccca 
      gtttgggga ttttttcaa cggcgccca aattatctt 
      cgacacgtt tgtatcaaa cggagaagt gaagtggtt 
      atggagtag cggtttagt tgtgcttct accacatgg 
      gtacgatgg agccggtcc tgaacacag ctcagccag 
      aatcgaaag gtttctaca aatgaccga ttccaaaga 
      agataaagt cgacggtga taatgttaa tatgcaagt 
      ttgtcatcc gagcacccc agcacaata tttccattt 
      actacgaag acagatgtt gcgcaattt tagaaaacc 
      tttaattgt aataggccc gaaaacact tttaagact 
      gccggaagc tacctcaaa tttcgccga catgggtcc 
      tcaaacaac atttcaacc cgtcataac cgataatac 
      tcacgaccc gaaacaaat ccaagtcgt tctgttaac 
      ctcaggaaa acactttta caatcttgc ttcaaaaag 
      ggaatcact aggtgccaa aaatgtggc aattgttcg 
      attagaatc gtattgccc gtttccgac tctagaatt 
      acaacagga agttgcgaa atttcccaa tgcaaaaat 
      ttttatgtg gtgtcaaga agagccaca aaacatggg 
      cgcttggag ttttgtgaa acctcgatt tgagaattt 
      agttggcag aaaaattca gttttgtgg tcgaccaac 
      tcaagctgc tccagcggt tggaattgg cgaattgca 
      cgaccagca agtgtcgga aatcctgag caaaccgtt 
      ttcgtttga aatttaaaa accgtttca attaattag 
      ccatatatt tttttatat aatgtacac agtacaata 
      cagtgctag ctatctacg aaaagacct tgtcagttg 
      ttctataag cctgacact taagtaata cttatctaa 
      cttaaattt agaattagt ataattata gaaaaggac 
      agtatttta caaacatga tatacacag acatcagtc 
      atatcaaaa cataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY