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Gene information
General information
TC015480 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9398853..9400573
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015480
    iBeetle screen iB_10592

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015480 RA
      MSHEEKYKS KDTNNETVC EIKKEKPDL DPPPDRFHL 
      VYILFFFLG LVHFLPWSF FTTATEFWM YKFRNTSIN 
      ETNSEFRTY LQAEFNASI NITLEITEV AFLIVGVLF 
      GHLIRVRVR VIGIFSIIF VLFAILSVF VEIDTDSWQ 
      EEFFGLVMV ITAGINSLN AVFTITVYT ILANFPQSY 
      LAPYLTGGT LARITTSSL QIFSLASGL SVQHSALLY 
      FLLGVGVVG LTLIFIIFT GKNKFYLHH MRNYVDGSK 
      EKRVSLKEG WRLLKIIWS PIIVMGLLI FTIDACPNA 
      LVVSEGEGQ GPWNDVYFI PVISYWLAG ICDLVGRNI 
      AFRFDLKLS NVGWIAFVA LRMVTMIPL FILANAQPR 
      HHLPVLLPH DYQYIIIVI VSNISGAYI MNRTYYNIK 
      KLVTQEELK DAYHVNMVM VGLQMGLFS CVNMVTVNL 
      L
    CDS sequence
    • >TC015480 RA
      atgtcacac gaagaaaaa tacaaaagt aaagacacc 
      aacaatgag acggtgtgt gaaattaag aaggaaaaa 
      ccggacctg gaccctccg ccagatcgg ttccaccta 
      gtttacatt ttatttttc ttcctgggg cttgttcat 
      ttcctgcct tggagcttt tttaccaca gcgactgaa 
      ttttggatg tacaagttc cgaaacact tcaattaat 
      gaaacgaat tcggaattt cggacgtac ctgcaggcg 
      gaatttaac gcctcgatt aacataact ttggaaata 
      acagaagtg gcttttttg atcgtgggg gttttgttc 
      gggcacttg atccgagtc agagtgcgg gtgattgga 
      attttttca ataatattc gtgcttttc gcgattttg 
      tccgttttt gtggaaatt gatacggat tcttggcaa 
      gaggagttt ttcgggctg gttatggtg atcacagct 
      ggaattaac tcactgaat gccgttttt acgatcact 
      gtgtacacg attttggcc aacttcccc caaagttat 
      ttggccccg tacttgaca gggggcact ttagcgaga 
      ataactact tccagtctt cagattttt tctctggct 
      tctggtttg tcggtgcaa cacagtgcc ttgctgtat 
      tttttgctg ggtgttggt gtggttggt ctaacacta 
      atttttatc attttcacc gggaaaaat aaattttat 
      ttgcaccac atgcgaaac tatgtcgat ggttctaag 
      gaaaagcga gtctctttg aaagagggc tggcgtttg 
      ttgaaaatt atatggtcg ccgattatt gtcatggga 
      cttcttata ttcacaatt gacgcttgt cccaatgct 
      ttggtcgtg tcagaaggc gagggacaa gggccttgg 
      aacgacgtt tacttcatt ccggttata agttactgg 
      ttggcagga atttgcgat ttggttgga cgaaacata 
      gcgtttaga ttcgatctt aaactgagc aatgttggt 
      tggatcgct tttgttgca ctaagaatg gttacgatg 
      ataccgttg tttatattg gctaatgcc caaccgcgt 
      caccattta ccagtgttg ctacctcat gattatcag 
      tatattatc attgtgatt gtatcgaat atttctggg 
      gcctatatc atgaaccga acgtactac aacatcaaa 
      aaattggtt actcaggaa gaactgaaa gacgcgtat 
      catgtcaat atggttatg gtagggctg cagatggga 
      ctgttttct tgtgtcaat atggttact gtgaacctt 
      ttgtaa
    mRNA sequence
    • >TC015480 RA
      ttctttgaa aaacaaaac cggaacaat gtcacacga 
      agaaaaata caaaagtaa agacaccaa caatgagac 
      ggtgtgtga aattaagaa ggaaaaacc ggacctgga 
      ccctccgcc agatcggtt ccacctagt ttacatttt 
      atttttctt cctggggct tgttcattt cctgccttg 
      gagcttttt taccacagc gactgaatt ttggatgta 
      caagttccg aaacacttc aattaatga aacgaattc 
      ggaatttcg gacgtacct gcaggcgga atttaacgc 
      ctcgattaa cataacttt ggaaataac agaagtggc 
      ttttttgat cgtgggggt tttgttcgg gcacttgat 
      ccgagtcag agtgcgggt gattggaat tttttcaat 
      aatattcgt gcttttcgc gattttgtc cgtttttgt 
      ggaaattga tacggattc ttggcaaga ggagttttt 
      cgggctggt tatggtgat cacagctgg aattaactc 
      actgaatgc cgtttttac gatcactgt gtacacgat 
      tttggccaa cttccccca aagttattt ggccccgta 
      cttgacagg gggcacttt agcgagaat aactacttc 
      cagtcttca gattttttc tctggcttc tggtttgtc 
      ggtgcaaca cagtgcctt gctgtattt tttgctggg 
      tgttggtgt ggttggtct aacactaat ttttatcat 
      tttcaccgg gaaaaataa attttattt gcaccacat 
      gcgaaacta tgtcgatgg ttctaagga aaagcgagt 
      ctctttgaa agagggctg gcgtttgtt gaaaattat 
      atggtcgcc gattattgt catgggact tcttatatt 
      cacaattga cgcttgtcc caatgcttt ggtcgtgtc 
      agaaggcga gggacaagg gccttggaa cgacgttta 
      cttcattcc ggttataag ttactggtt ggcaggaat 
      ttgcgattt ggttggacg aaacatagc gtttagatt 
      cgatcttaa actgagcaa tgttggttg gatcgcttt 
      tgttgcact aagaatggt tacgatgat accgttgtt 
      tatattggc taatgccca accgcgtca ccatttacc 
      agtgttgct acctcatga ttatcagta tattatcat 
      tgtgattgt atcgaatat ttctggggc ctatatcat 
      gaaccgaac gtactacaa catcaaaaa attggttac 
      tcaggaaga actgaaaga cgcgtatca tgtcaatat 
      ggttatggt agggctgca gatgggact gttttcttg 
      tgtcaatat ggttactgt gaacctttt gtaattgag 
      ctgttttaa gcttgtctt tgatcttaa tctgtttac 
      ttttaatat tgttatacc gtaaagttt tgattattg 
      aatacgatt caatttttt tgagttgta ttaaaagtt 
      tattaattt atcaaattt tttcggagt tgtaataaa 
      aatttatta gactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY