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Gene information
General information
TC014364 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9751880..9755416
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014364
    iBeetle screen iB_10591

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature potentially thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna antennal flagellum shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs potentially number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen cuticle ectopically structure present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014364 RA
      MSRFAKVEQ GPPIEVFAL NQQFAADTS PNKVSLGVG 
      AYRTNEGQP WVLPVVREA EKILAADEK LNKEYLPVL 
      GLDTFSSAA TQMLLGADS PALKENRAF GVQCLSGTG 
      CLRVGAEFL ARTVGYKTF YVSIPTWEN HKLVFTNAG 
      FNDIKEYRY WSSESRGLD LKGFLEDLN KAPENSVII 
      LHSCAHNPT GCDPTQEQW AQIADVMEK RKLFPFFDS 
      AYQGFASGD LEKDAWTVR YFVSRGFEL LCAQSFAKN 
      FGLYNERVG NLTFVTKTT DVIPKVKSQ VTLLVRGMY 
      SNPPSHGAR IVAHVLSDP KLFEQWKGC IRTMATRII 
      EMRKALRAA LEKLNTPGD WSHITAQIG MFSYTGLTE 
      KQSLHMVEK HHIYMLKSG RISMCGVTP GNVDYVAKA 
      IYETVTNVK
    • >TC014364 RB
      MSRFAKVEQ GPPIEVFAL NQQFAADTS PNKVSLGVG 
      AYRTNEGQP WVLPVVREA EKILAADEK LNKEYLPVL 
      GLDTFSSAA TQMLLGADS PALKENRAF GVQCLSGTG 
      CLRVGAEFL ARTVGYKTF YVSIPTWEN HKLVFTNAG 
      FNDIKEYRY WSSESRGLD LKGFLEDLN KAPENSVII 
      LHSCAHNPT GCDPTQEQW AQIADVMEK RKLFPFFDS 
      AYQGFASGD LEKDAWTVR YFVSRGFEL LCAQSFAKN 
      FGLYNERVG NLTFVTKTT DVIPKVKSQ VTLLVRGMY 
      SNPPSHGAR IVAHVLSDP KLFEQWKGC IRTMATRII 
      EMRKALRAA LEKLNTPGD WSHITAQIG MFSYTGLTE 
      KQSLHMVEK HHIYMLKSG RISMCGVTP GNVDYVAKA 
      IYETVTNCH QTLGNKL
    CDS sequence
    • >TC014364 RA
      atgtcaaga ttcgccaaa gtggagcaa gggcccccc 
      atcgaggtc ttcgccttg aaccagcaa ttcgccgcc 
      gacacgagc ccgaataaa gtcagtttg ggggttgga 
      gcgtatcgt actaatgag ggtcaacct tgggttttg 
      ccagtggtt cgcgaagcc gaaaaaatc cttgctgcc 
      gatgagaaa ctgaacaag gagtatttg ccggttttg 
      ggcttggac actttcagc tcggcggcc actcagatg 
      ctgctgggg gctgatagt cccgctctc aaggaaaat 
      cgggctttt ggggtccag tgcctatcc ggcacgggc 
      tgtcttcgc gttggggct gaatttctg gcccgaacc 
      gtcggctac aaaaccttc tacgtgtcc atacccacc 
      tgggagaac cacaaactc gtattcacc aacgccggc 
      ttcaacgac atcaaagaa tacagatac tggtcatcg 
      gagtcccgc ggcctcgac ctcaaaggc tttctggaa 
      gacctcaac aaagcgcct gaaaactca gtcataatc 
      ctgcactcc tgcgcccat aatcccaca ggatgcgac 
      cccacgcag gaacagtgg gcccaaatc gccgatgtt 
      atggaaaag aggaaactc ttcccgttt ttcgattcc 
      gcgtatcag ggctttgcc tcaggcgat ctggagaag 
      gacgcctgg acggtcaga tatttcgtc agcagggga 
      tttgagcta ttgtgtgca caatcattc gcgaaaaat 
      ttcggcttg tacaacgaa cgtgttggt aatttgact 
      tttgtgacc aaaacgacc gatgttatc cccaaagtt 
      aagtcacag gtgacttta ctagtgagg gggatgtac 
      tcgaacccg cctagtcac ggtgctaga attgtggca 
      cacgtttta agcgatcca aagttgttc gaacagtgg 
      aaaggttgt attaggacg atggcgacc aggattatc 
      gaaatgaga aaagctttg agggcagct ttggaaaaa 
      ttgaacact ccgggagac tggagtcat atcacggcg 
      caaatcggc atgttttcg tacacggga ctgactgaa 
      aaacaatcc ttgcacatg gttgagaaa caccacatc 
      tatatgttg aaatcgggc cgtatcagc atgtgtgga 
      gtcactcca ggcaatgtg gattacgtg gccaaagct 
      atttatgaa actgtaaca aatgtaaag taa
    • >TC014364 RB
      atgtcaaga ttcgccaaa gtggagcaa gggcccccc 
      atcgaggtc ttcgccttg aaccagcaa ttcgccgcc 
      gacacgagc ccgaataaa gtcagtttg ggggttgga 
      gcgtatcgt actaatgag ggtcaacct tgggttttg 
      ccagtggtt cgcgaagcc gaaaaaatc cttgctgcc 
      gatgagaaa ctgaacaag gagtatttg ccggttttg 
      ggcttggac actttcagc tcggcggcc actcagatg 
      ctgctgggg gctgatagt cccgctctc aaggaaaat 
      cgggctttt ggggtccag tgcctatcc ggcacgggc 
      tgtcttcgc gttggggct gaatttctg gcccgaacc 
      gtcggctac aaaaccttc tacgtgtcc atacccacc 
      tgggagaac cacaaactc gtattcacc aacgccggc 
      ttcaacgac atcaaagaa tacagatac tggtcatcg 
      gagtcccgc ggcctcgac ctcaaaggc tttctggaa 
      gacctcaac aaagcgcct gaaaactca gtcataatc 
      ctgcactcc tgcgcccat aatcccaca ggatgcgac 
      cccacgcag gaacagtgg gcccaaatc gccgatgtt 
      atggaaaag aggaaactc ttcccgttt ttcgattcc 
      gcgtatcag ggctttgcc tcaggcgat ctggagaag 
      gacgcctgg acggtcaga tatttcgtc agcagggga 
      tttgagcta ttgtgtgca caatcattc gcgaaaaat 
      ttcggcttg tacaacgaa cgtgttggt aatttgact 
      tttgtgacc aaaacgacc gatgttatc cccaaagtt 
      aagtcacag gtgacttta ctagtgagg gggatgtac 
      tcgaacccg cctagtcac ggtgctaga attgtggca 
      cacgtttta agcgatcca aagttgttc gaacagtgg 
      aaaggttgt attaggacg atggcgacc aggattatc 
      gaaatgaga aaagctttg agggcagct ttggaaaaa 
      ttgaacact ccgggagac tggagtcat atcacggcg 
      caaatcggc atgttttcg tacacggga ctgactgaa 
      aaacaatcc ttgcacatg gttgagaaa caccacatc 
      tatatgttg aaatcgggc cgtatcagc atgtgtgga 
      gtcactcca ggcaatgtg gattacgtg gccaaagct 
      atttatgaa actgtaaca aattgtcac caaactttg 
      gggaacaaa ctgtaa
    mRNA sequence
    • >TC014364 RA
      cgggtcaaa aaggttatc tcaatcaaa ttctaatac 
      tcaaaacag tgcaaattt tcggcgttt tccccgaga 
      tcgcgagca gtgaacatg tcaagattc gccaaagtg 
      gagcaaggg ccccccatc gaggtcttc gccttgaac 
      cagcaattc gccgccgac acgagcccg aataaagtc 
      agtttgggg gttggagcg tatcgtact aatgagggt 
      caaccttgg gttttgcca gtggttcgc gaagccgaa 
      aaaatcctt gctgccgat gagaaactg aacaaggag 
      tatttgccg gttttgggc ttggacact ttcagctcg 
      gcggccact cagatgctg ctgggggct gatagtccc 
      gctctcaag gaaaatcgg gcttttggg gtccagtgc 
      ctatccggc acgggctgt cttcgcgtt ggggctgaa 
      tttctggcc cgaaccgtc ggctacaaa accttctac 
      gtgtccata cccacctgg gagaaccac aaactcgta 
      ttcaccaac gccggcttc aacgacatc aaagaatac 
      agatactgg tcatcggag tcccgcggc ctcgacctc 
      aaaggcttt ctggaagac ctcaacaaa gcgcctgaa 
      aactcagtc ataatcctg cactcctgc gcccataat 
      cccacagga tgcgacccc acgcaggaa cagtgggcc 
      caaatcgcc gatgttatg gaaaagagg aaactcttc 
      ccgtttttc gattccgcg tatcagggc tttgcctca 
      ggcgatctg gagaaggac gcctggacg gtcagatat 
      ttcgtcagc aggggattt gagctattg tgtgcacaa 
      tcattcgcg aaaaatttc ggcttgtac aacgaacgt 
      gttggtaat ttgactttt gtgaccaaa acgaccgat 
      gttatcccc aaagttaag tcacaggtg actttacta 
      gtgaggggg atgtactcg aacccgcct agtcacggt 
      gctagaatt gtggcacac gttttaagc gatccaaag 
      ttgttcgaa cagtggaaa ggttgtatt aggacgatg 
      gcgaccagg attatcgaa atgagaaaa gctttgagg 
      gcagctttg gaaaaattg aacactccg ggagactgg 
      agtcatatc acggcgcaa atcggcatg ttttcgtac 
      acgggactg actgaaaaa caatccttg cacatggtt 
      gagaaacac cacatctat atgttgaaa tcgggccgt 
      atcagcatg tgtggagtc actccaggc aatgtggat 
      tacgtggcc aaagctatt tatgaaact gtaacaaat 
      gtaaagtaa caaattatt tatttttac tataatatt 
      gtgtattat accgaaacc aaatttttt acaatgttt 
      tgtaattgg atgtatatg taaataaaa gtccggact 
      gaaagctac atttttgca atgttttat aatgcattt 
      catattgtg ataaatgat gtaacagaa catttccgt 
      gtttttttt tacacatga tctttattt gcagtgtca 
      ccaaacttt ggggaacaa actgtaaaa atggtaaag 
      gacggatta ttttctaag tattatatt gtttctaca 
      ctggtgatt aaaataaat agaaaatgt attt
    • >TC014364 RB
      cgggtcaaa aaggttatc tcaatcaaa ttctaatac 
      tcaaaacag tgcaaattt tcggcgttt tccccgaga 
      tcgcgagca gtgaacatg tcaagattc gccaaagtg 
      gagcaaggg ccccccatc gaggtcttc gccttgaac 
      cagcaattc gccgccgac acgagcccg aataaagtc 
      agtttgggg gttggagcg tatcgtact aatgagggt 
      caaccttgg gttttgcca gtggttcgc gaagccgaa 
      aaaatcctt gctgccgat gagaaactg aacaaggag 
      tatttgccg gttttgggc ttggacact ttcagctcg 
      gcggccact cagatgctg ctgggggct gatagtccc 
      gctctcaag gaaaatcgg gcttttggg gtccagtgc 
      ctatccggc acgggctgt cttcgcgtt ggggctgaa 
      tttctggcc cgaaccgtc ggctacaaa accttctac 
      gtgtccata cccacctgg gagaaccac aaactcgta 
      ttcaccaac gccggcttc aacgacatc aaagaatac 
      agatactgg tcatcggag tcccgcggc ctcgacctc 
      aaaggcttt ctggaagac ctcaacaaa gcgcctgaa 
      aactcagtc ataatcctg cactcctgc gcccataat 
      cccacagga tgcgacccc acgcaggaa cagtgggcc 
      caaatcgcc gatgttatg gaaaagagg aaactcttc 
      ccgtttttc gattccgcg tatcagggc tttgcctca 
      ggcgatctg gagaaggac gcctggacg gtcagatat 
      ttcgtcagc aggggattt gagctattg tgtgcacaa 
      tcattcgcg aaaaatttc ggcttgtac aacgaacgt 
      gttggtaat ttgactttt gtgaccaaa acgaccgat 
      gttatcccc aaagttaag tcacaggtg actttacta 
      gtgaggggg atgtactcg aacccgcct agtcacggt 
      gctagaatt gtggcacac gttttaagc gatccaaag 
      ttgttcgaa cagtggaaa ggttgtatt aggacgatg 
      gcgaccagg attatcgaa atgagaaaa gctttgagg 
      gcagctttg gaaaaattg aacactccg ggagactgg 
      agtcatatc acggcgcaa atcggcatg ttttcgtac 
      acgggactg actgaaaaa caatccttg cacatggtt 
      gagaaacac cacatctat atgttgaaa tcgggccgt 
      atcagcatg tgtggagtc actccaggc aatgtggat 
      tacgtggcc aaagctatt tatgaaact gtaacaaat 
      tgtcaccaa actttgggg aacaaactg taaaaatgg 
      taaaggacg gattatttt ctaagtatt atattgttt 
      ctacactgg tgattaaaa taaatagaa aatgtattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY