| General information | |||
| TC010878 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 5004575..5011554 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010878 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10589 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Days post injection
Analysis of embryonic musculature and early development
number of eggs/embryos/larvae on slide: > 50
larval musculature pattern irregular -
Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: > 50
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): more than 80%
larva segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
larva appendages swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
head appendages swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
thorax segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): more than 80%
larva segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
larva appendages swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
head appendages swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
thorax segment potentially number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC010878 RA
MNLNWFAHI IVHHPYIII SAVAVFSGT CLVIPFTLK TKPFKDFSD PKMGFTTRG TAISNRLTA WKNLDESTR PSGQFAINP KDILIKRNV TFFKPKVKK NGKKKKNKQ KNDFSNVFI NEASNNRSD NSYENNNWR ALQNMLHPK AAENVAVKP VEIDGFFCG YPDERFAHI VLKSKNAED LFTLKSLLA LCRIEHEFI EAHYYKDLC ISNQDYHVK KCCKPWSLA NYIAILHNR TSCLAITEE DVNATKTLL INCSQYFHK LQLMQDCFD KGYCDVPLE CVRHDAVFN VLNFLTSTT FLPPNNTRE STTLEETMI FLPLACSTA ILPYYHEII QMDLEYEGI SVVAMEFGI KDALFDECL LRDAWLMTS GALFVLLCM WLYTESLFL TIMTIIAII FSLGISYFM YMLVFELKF FPFMNLLAI IVAIGIGAD DAFIFCKIW HKVKEEKNS SIVKLMGDT FYHAFFSMF VTSLTTSVA FLASYASSV TAICCFSIF SGTAVITNF FMMITWFPA CVVIWERSC CSNVDLAKS CLMACLQRW CCSKLIKPT WNLSTTWPK CSFLGRIWS SKERFLLDS VVNFRYIWL VILSIIGLI STIVVIYYP KFQLPDSSE FQLFDSSHP FEQYDFKYK YHFWFRREE RENLVGVNY KLPLRFVWG VLPVDNGDH LNPESYGKL ELDPDFDIS QPESQVWLL KFCKRLQRQ PFFQPTFGP LLPNCFIET FMRSMQRDC VDSFSQRDW SPCCKMSTF PYNSSVFYE CIVEEMTDI YETPSQYLI PGMAGPKFS KDQFPTIKA VIVEYDSNY SYSMSYEYM HEFYTKVEN WMIEELKTA PKTMKNGWF ISELEFYDL QRELSENTI LAIIVSMGL ALIVLLLST LNILTSLYA ILTITCSIF VTVAVLVLL GWKLNILES ITVSTAIGL TVDFSLHYT VNYRLCPLA LADERIAAT KYALSYMAG PVLMAALTT GAAGAFMMP SLILPYIQI GIFLVTVMS VSWIYATFH LGAMLAIIG PQNQFGQFH YSKLSCCPS SKSKTSSSD RNRPVNHSS LSDTHELDS LTCKSGVRP IPRPLQRSL STGGAGKNN HNRYVFTDQ SPSATSAIT IIMADDN
- >TC010878 RB
MNLNWFAHI IVHHPYIII SAVAVFSGT CLVIPFTLK TKPFKDFSD PKMGFTTRG TAISNRLTA WKNLDESTR PSGQFAINP KDILIKRNV TFFKPKVKK NGKKKKNKQ KNDFSNVTL PLKSPPSKP PFQVFINEA SNNRSDNSY ENNNWRALQ NMLHPKAAE NVAVKPVEI DGFFCGYPD ERFAHIVLK SKNAEDLFT LKSLLALCR IEHEFIEAH YYKDLCISN QDYHVKKCC KPWSLANYI AILHNRTSC LAITEEDVN ATKTLLINC SQYFHKLQL MQDCFDKGY CDVPLECVR HDAVFNVLN FLTSTTFLP PNNTRESTT LEETMIFLP LACSTAILP YYHEIIQMD LEYEGISVV AMEFGIKDA LFDECLLRD AWLMTSGAL FVLLCMWLY TESLFLTIM TIIAIIFSL GISYFMYML VFELKFFPF MNLLAIIVA IGIGADDAF IFCKIWHKV KEEKNSSIV KLMGDTFYH AFFSMFVTS LTTSVAFLA SYASSVTAI CCFSIFSGT AVITNFFMM ITWFPACVV IWERSCCSN VDLAKSCLM ACLQRWCCS KLIKPTWNL STTWPKCSF LGRIWSSKE RFLLDSVVN FRYIWLVIL SIIGLISTI VVIYYPKFQ LPDSSEFQL FDSSHPFEQ YDFKYKYHF WFRREEREN LVGVNYKLP LRFVWGVLP VDNGDHLNP ESYGKLELD PDFDISQPE SQVWLLKFC KRLQRQPFF QPTFGPLLP NCFIETFMR SMQRDCVDS FSQRDWSPC CKMSTFPYN SSVFYECIV EEMTDIYET PSQYLIPGM AGPKFSKDQ FPTIKAVIV EYDSNYSYS MSYEYMHEF YTKVENWMI EELKTAPKT MKNGWFISE LEFYDLQRE LSENTILAI IVSMGLALI VLLLSTLNI LTSLYAILT ITCSIFVTV AVLVLLGWK LNILESITV STAIGLTVD FSLHYTVNY RLCPLALAD ERIAATKYA LSYMAGPVL MAALTTGAA GAFMMPSLI LPYIQIGIF LVTVMSVSW IYATFHLGA MLAIIGPQN QFGQFHYSK LSCCPSSKS KTSSSDRNR PVNHSSLSD THELDSLTC KSGVRPIPR PLQRSLSTG GAGKNNHNR YVFTDQSPS ATSAITIIM ADDN
- >TC010878 RA
atgaatttg aattggttc gcacatatt atcgtccat cacccctac atcattata tcggcagtt gctgtgttc tctggtaca tgcctggtg atacctttt acactgaaa accaagccc ttcaaagac ttttcggac cccaaaatg ggcttcact accagagga acagctata tcaaacaga ctaactgct tggaagaac ttagatgaa tcaactcga ccatcagga caatttgct attaacccc aaagatatt ttgataaaa cgaaatgta acatttttt aaacctaaa gttaagaaa aatggaaaa aagaagaaa aacaaacaa aaaaacgat ttttctaat gtatttata aatgaagca agtaataat cgctctgac aatagctac gaaaataat aactggagg gctctacaa aacatgttg cacccaaaa gctgctgaa aacgtcgcc gtcaaaccg gtcgaaatc gacggtttt ttttgtggt tatcctgac gaacgattc gctcacata gtattaaaa tcgaaaaac gccgaggac ctgtttact ttaaaatct ttactggct ctgtgtcgc atagagcac gaatttatt gaagctcac tattacaaa gacttgtgc atttcgaat caggattac cacgtaaag aagtgctgc aagccatgg tctttagcc aattacata gcaatttta cacaataga acttcgtgt ttagcaatt acggaagaa gacgttaac gcaacaaaa acattattg ataaattgt tcgcaatat tttcataaa ctgcaacta atgcaagat tgcttcgat aagggatac tgtgatgta ccgcttgaa tgcgttcga cacgatgcc gtttttaat gtacttaat tttttaaca agcacgacg tttctgccg cctaataac actcgggag agtacaacg ttagaagaa acaatgatc tttctacca ctagcatgt agtacagca attttacca tattatcac gagattatt caaatggac ttagaatat gaaggcata tcagttgta gcaatggaa tttgggata aaagatgct ctgtttgac gagtgcctg ttacgggac gcttggttg atgacttcc ggggcactc tttgtgtta ttatgcatg tggctatat acggaatca ttgttcctg actatcatg acaataatt gcaattata ttctcactg gggatttcg tattttatg tacatgctc gtttttgag ctgaaattt tttccgttt atgaatttg ctagctatt attgttgca atcggtatt ggagcggac gatgctttt atattttgt aaaatatgg cacaaagtt aaagaagaa aaaaatagc tcgatagtc aaactaatg ggtgatacc ttctatcat gcatttttt tcgatgttt gttacgtcc ttaacgacc tcggtggct ttcctggcc tcctatgcc agctctgtg acggcaata tgttgtttc agcatattt tcggggacg gccgtaata acgaatttt tttatgatg atcacttgg ttccccgca tgtgttgta atatgggag cgctcatgt tgttcaaat gttgattta gcgaaaagt tgtttaatg gcgtgcttg caacgttgg tgttgttca aaactgata aaaccgact tggaactta tcaacgaca tggccgaaa tgttcattt ttggggaga atctggagt tccaaagag cgtttcttg ttagacagt gttgtcaat ttcagatat atatggttg gttatattg tccataata ggtttaata agtacgatc gttgtgatt tattatccg aaatttcaa ctacctgac tcatccgaa ttccaactg ttcgacagc tcgcacccc ttcgaacaa tacgatttt aaatacaaa tatcacttt tggttcaga cgcgaggaa agggagaat ttggttggc gttaattac aagcttccg ttacgtttt gtctggggt gttttaccc gtggacaat ggcgaccat ctgaacccg gagagttac ggcaaattg gagctggat ccggacttc gatatatct caaccggaa tctcaagtg tggctcttg aagttttgc aagcgcttg caacgccag ccctttttc caacccacc tttgggccc cttttgcca aactgtttc attgaaacg ttcatgcgc tcgatgcag agggattgt gttgactcg ttttctcaa agagattgg tcaccttgt tgcaaaatg tccacgttt ccgtataac agtagcgtg ttttatgaa tgcatagtg gaagagatg acggatatt tacgaaaca cctagtcaa tatttgata cctggaatg gccggtccg aaattttct aaagaccaa tttccgaca ataaaagct gttatcgta gagtatgat agtaattat agttattca atgtcttat gaatacatg catgaattt tacacgaaa gtcgagaac tggatgatt gaggagctg aagacggct cccaagaca atgaaaaac ggctggttc atcagcgag ttggagttc tacgacttg cagagagag ttatcggaa aatactata ttagcaatt atagtgtca atgggatta gctttaata gtcttgctt ctatcaaca ttgaatatt ttgacaagt ttatacgcg attttaact ataacgtgc agtattttt gtaactgtt gcagtacta gttctgtta gggtggaag ttgaatatt ttggagtct ataactgtc tcgacagct ataggtctt actgtagat tttagttta cattacact gtgaattat agattatgt ccacttgct ctggcagac gagcggatc gctgctacc aagtacgca ttgtcttac atggcaggt cctgtattg atggcagca ttaacaacc ggggcggct ggagcattc atgatgccc tcactaatc ttgccatac atacagatc ggaatattc cttgtaact gtaatgagt gttagttgg atatatgcc acattccat ctgggtgct atgttggcc attattgga ccccagaat cagttcggc caattccac tattccaag cttagttgt tgtccttcc agtaaatca aaaacgtcc tctagcgac cgaaatcgt cctgttaat cactcctct ctctcagat acacacgaa ttagattcg ttgacttgc aagagtgga gtacggcct attccgaga cccctccaa aggtctctc agtactggc ggggctggc aaaaacaac cacaataga tatgttttt accgatcaa tcaccctcc gccaccagc gccattact attatcatg gccgatgac aattaa
- >TC010878 RB
atgaatttg aattggttc gcacatatt atcgtccat cacccctac atcattata tcggcagtt gctgtgttc tctggtaca tgcctggtg atacctttt acactgaaa accaagccc ttcaaagac ttttcggac cccaaaatg ggcttcact accagagga acagctata tcaaacaga ctaactgct tggaagaac ttagatgaa tcaactcga ccatcagga caatttgct attaacccc aaagatatt ttgataaaa cgaaatgta acatttttt aaacctaaa gttaagaaa aatggaaaa aagaagaaa aacaaacaa aaaaacgat ttttctaat gtaactttg ccgctcaag tcgccccct tctaaacca ccatttcag gtatttata aatgaagca agtaataat cgctctgac aatagctac gaaaataat aactggagg gctctacaa aacatgttg cacccaaaa gctgctgaa aacgtcgcc gtcaaaccg gtcgaaatc gacggtttt ttttgtggt tatcctgac gaacgattc gctcacata gtattaaaa tcgaaaaac gccgaggac ctgtttact ttaaaatct ttactggct ctgtgtcgc atagagcac gaatttatt gaagctcac tattacaaa gacttgtgc atttcgaat caggattac cacgtaaag aagtgctgc aagccatgg tctttagcc aattacata gcaatttta cacaataga acttcgtgt ttagcaatt acggaagaa gacgttaac gcaacaaaa acattattg ataaattgt tcgcaatat tttcataaa ctgcaacta atgcaagat tgcttcgat aagggatac tgtgatgta ccgcttgaa tgcgttcga cacgatgcc gtttttaat gtacttaat tttttaaca agcacgacg tttctgccg cctaataac actcgggag agtacaacg ttagaagaa acaatgatc tttctacca ctagcatgt agtacagca attttacca tattatcac gagattatt caaatggac ttagaatat gaaggcata tcagttgta gcaatggaa tttgggata aaagatgct ctgtttgac gagtgcctg ttacgggac gcttggttg atgacttcc ggggcactc tttgtgtta ttatgcatg tggctatat acggaatca ttgttcctg actatcatg acaataatt gcaattata ttctcactg gggatttcg tattttatg tacatgctc gtttttgag ctgaaattt tttccgttt atgaatttg ctagctatt attgttgca atcggtatt ggagcggac gatgctttt atattttgt aaaatatgg cacaaagtt aaagaagaa aaaaatagc tcgatagtc aaactaatg ggtgatacc ttctatcat gcatttttt tcgatgttt gttacgtcc ttaacgacc tcggtggct ttcctggcc tcctatgcc agctctgtg acggcaata tgttgtttc agcatattt tcggggacg gccgtaata acgaatttt tttatgatg atcacttgg ttccccgca tgtgttgta atatgggag cgctcatgt tgttcaaat gttgattta gcgaaaagt tgtttaatg gcgtgcttg caacgttgg tgttgttca aaactgata aaaccgact tggaactta tcaacgaca tggccgaaa tgttcattt ttggggaga atctggagt tccaaagag cgtttcttg ttagacagt gttgtcaat ttcagatat atatggttg gttatattg tccataata ggtttaata agtacgatc gttgtgatt tattatccg aaatttcaa ctacctgac tcatccgaa ttccaactg ttcgacagc tcgcacccc ttcgaacaa tacgatttt aaatacaaa tatcacttt tggttcaga cgcgaggaa agggagaat ttggttggc gttaattac aagcttccg ttacgtttt gtctggggt gttttaccc gtggacaat ggcgaccat ctgaacccg gagagttac ggcaaattg gagctggat ccggacttc gatatatct caaccggaa tctcaagtg tggctcttg aagttttgc aagcgcttg caacgccag ccctttttc caacccacc tttgggccc cttttgcca aactgtttc attgaaacg ttcatgcgc tcgatgcag agggattgt gttgactcg ttttctcaa agagattgg tcaccttgt tgcaaaatg tccacgttt ccgtataac agtagcgtg ttttatgaa tgcatagtg gaagagatg acggatatt tacgaaaca cctagtcaa tatttgata cctggaatg gccggtccg aaattttct aaagaccaa tttccgaca ataaaagct gttatcgta gagtatgat agtaattat agttattca atgtcttat gaatacatg catgaattt tacacgaaa gtcgagaac tggatgatt gaggagctg aagacggct cccaagaca atgaaaaac ggctggttc atcagcgag ttggagttc tacgacttg cagagagag ttatcggaa aatactata ttagcaatt atagtgtca atgggatta gctttaata gtcttgctt ctatcaaca ttgaatatt ttgacaagt ttatacgcg attttaact ataacgtgc agtattttt gtaactgtt gcagtacta gttctgtta gggtggaag ttgaatatt ttggagtct ataactgtc tcgacagct ataggtctt actgtagat tttagttta cattacact gtgaattat agattatgt ccacttgct ctggcagac gagcggatc gctgctacc aagtacgca ttgtcttac atggcaggt cctgtattg atggcagca ttaacaacc ggggcggct ggagcattc atgatgccc tcactaatc ttgccatac atacagatc ggaatattc cttgtaact gtaatgagt gttagttgg atatatgcc acattccat ctgggtgct atgttggcc attattgga ccccagaat cagttcggc caattccac tattccaag cttagttgt tgtccttcc agtaaatca aaaacgtcc tctagcgac cgaaatcgt cctgttaat cactcctct ctctcagat acacacgaa ttagattcg ttgacttgc aagagtgga gtacggcct attccgaga cccctccaa aggtctctc agtactggc ggggctggc aaaaacaac cacaataga tatgttttt accgatcaa tcaccctcc gccaccagc gccattact attatcatg gccgatgac aattaa
- >TC010878 RA
gggaatgca ctgatagta accttgact tgttggtat gagagtggc tgtgtcctt gaagtattg tatttgttt ataaacagc tgacttcca tctcgcctg gccttgata ccgcgctgg gagccgccc ggttcgggc gcaatcggc gggctccgc tcggtgctt caccggcga ttttctcgg aactcgtgc gattaaagt ggatttttc gcgttttat tcgcacagc cgtcgcctt gttcgtcgg aggaatgtg actccaggc tgggttttt cggtgctgg ctgcggcaa ttgtctggt ggggttcca cgaagcggt cgcgttttg gtggttagt ttacccgtt accgcaccc ggctgcccg gacgagcga tccagggtg gctttggct ccatcaaat aggccctaa aatgaccgg ttttcgcac aattcagcg cgcggtttt gatattaca agcaatttt tcgacgagg gactagaag caatctcaa atggtaaat ggagtaagc gttaaaaat gaatttgaa ttggttcgc acatattat cgtccatca cccctacat cattatatc ggcagttgc tgtgttctc tggtacatg cctggtgat accttttac actgaaaac caagccctt caaagactt ttcggaccc caaaatggg cttcactac cagaggaac agctatatc aaacagact aactgcttg gaagaactt agatgaatc aactcgacc atcaggaca atttgctat taaccccaa agatatttt gataaaacg aaatgtaac attttttaa acctaaagt taagaaaaa tggaaaaaa gaagaaaaa caaacaaaa aaacgattt ttctaatgt atttataaa tgaagcaag taataatcg ctctgacaa tagctacga aaataataa ctggagggc tctacaaaa catgttgca cccaaaagc tgctgaaaa cgtcgccgt caaaccggt cgaaatcga cggtttttt ttgtggtta tcctgacga acgattcgc tcacatagt attaaaatc gaaaaacgc cgaggacct gtttacttt aaaatcttt actggctct gtgtcgcat agagcacga atttattga agctcacta ttacaaaga cttgtgcat ttcgaatca ggattacca cgtaaagaa gtgctgcaa gccatggtc tttagccaa ttacatagc aattttaca caatagaac ttcgtgttt agcaattac ggaagaaga cgttaacgc aacaaaaac attattgat aaattgttc gcaatattt tcataaact gcaactaat gcaagattg cttcgataa gggatactg tgatgtacc gcttgaatg cgttcgaca cgatgccgt ttttaatgt acttaattt tttaacaag cacgacgtt tctgccgcc taataacac tcgggagag tacaacgtt agaagaaac aatgatctt tctaccact agcatgtag tacagcaat tttaccata ttatcacga gattattca aatggactt agaatatga aggcatatc agttgtagc aatggaatt tgggataaa agatgctct gtttgacga gtgcctgtt acgggacgc ttggttgat gacttccgg ggcactctt tgtgttatt atgcatgtg gctatatac ggaatcatt gttcctgac tatcatgac aataattgc aattatatt ctcactggg gatttcgta ttttatgta catgctcgt ttttgagct gaaattttt tccgtttat gaatttgct agctattat tgttgcaat cggtattgg agcggacga tgcttttat attttgtaa aatatggca caaagttaa agaagaaaa aaatagctc gatagtcaa actaatggg tgatacctt ctatcatgc atttttttc gatgtttgt tacgtcctt aacgacctc ggtggcttt cctggcctc ctatgccag ctctgtgac ggcaatatg ttgtttcag catattttc ggggacggc cgtaataac gaatttttt tatgatgat cacttggtt ccccgcatg tgttgtaat atgggagcg ctcatgttg ttcaaatgt tgatttagc gaaaagttg tttaatggc gtgcttgca acgttggtg ttgttcaaa actgataaa accgacttg gaacttatc aacgacatg gccgaaatg ttcattttt ggggagaat ctggagttc caaagagcg tttcttgtt agacagtgt tgtcaattt cagatatat atggttggt tatattgtc cataatagg tttaataag tacgatcgt tgtgattta ttatccgaa atttcaact acctgactc atccgaatt ccaactgtt cgacagctc gcacccctt cgaacaata cgattttaa atacaaata tcacttttg gttcagacg cgaggaaag ggagaattt ggttggcgt taattacaa gcttccgtt acgttttgt ctggggtgt tttacccgt ggacaatgg cgaccatct gaacccgga gagttacgg caaattgga gctggatcc ggacttcga tatatctca accggaatc tcaagtgtg gctcttgaa gttttgcaa gcgcttgca acgccagcc ctttttcca acccacctt tgggcccct tttgccaaa ctgtttcat tgaaacgtt catgcgctc gatgcagag ggattgtgt tgactcgtt ttctcaaag agattggtc accttgttg caaaatgtc cacgtttcc gtataacag tagcgtgtt ttatgaatg catagtgga agagatgac ggatattta cgaaacacc tagtcaata tttgatacc tggaatggc cggtccgaa attttctaa agaccaatt tccgacaat aaaagctgt tatcgtaga gtatgatag taattatag ttattcaat gtcttatga atacatgca tgaatttta cacgaaagt cgagaactg gatgattga ggagctgaa gacggctcc caagacaat gaaaaacgg ctggttcat cagcgagtt ggagttcta cgacttgca gagagagtt atcggaaaa tactatatt agcaattat agtgtcaat gggattagc tttaatagt cttgcttct atcaacatt gaatatttt gacaagttt atacgcgat tttaactat aacgtgcag tatttttgt aactgttgc agtactagt tctgttagg gtggaagtt gaatatttt ggagtctat aactgtctc gacagctat aggtcttac tgtagattt tagtttaca ttacactgt gaattatag attatgtcc acttgctct ggcagacga gcggatcgc tgctaccaa gtacgcatt gtcttacat ggcaggtcc tgtattgat ggcagcatt aacaaccgg ggcggctgg agcattcat gatgccctc actaatctt gccatacat acagatcgg aatattcct tgtaactgt aatgagtgt tagttggat atatgccac attccatct gggtgctat gttggccat tattggacc ccagaatca gttcggcca attccacta ttccaagct tagttgttg tccttccag taaatcaaa aacgtcctc tagcgaccg aaatcgtcc tgttaatca ctcctctct ctcagatac acacgaatt agattcgtt gacttgcaa gagtggagt acggcctat tccgagacc cctccaaag gtctctcag tactggcgg ggctggcaa aaacaacca caatagata tgtttttac cgatcaatc accctccgc caccagcgc cattactat tatcatggc cgatgacaa ttaaatgta acattagga ctaaatgtt ctgtgatat actctgtat ttcttttat ttttatttc gatttttac ataaatgct ctttcaaca actatcatt tacccttgt tcaaatggc atttaagta acaaacgta cacatatat aaattaaat atatattat agaaaggac cacgctctt tcagtgcac ccataccat ccccctcct ccaattttt aagcaaacg aaagaattt tgacgatca attaataaa acgttcttt aaatt
- >TC010878 RB
gggaatgca ctgatagta accttgact tgttggtat gagagtggc tgtgtcctt gaagtattg tatttgttt ataaacagc tgacttcca tctcgcctg gccttgata ccgcgctgg gagccgccc ggttcgggc gcaatcggc gggctccgc tcggtgctt caccggcga ttttctcgg aactcgtgc gattaaagt ggatttttc gcgttttat tcgcacagc cgtcgcctt gttcgtcgg aggaatgtg actccaggc tgggttttt cggtgctgg ctgcggcaa ttgtctggt ggggttcca cgaagcggt cgcgttttg gtggttagt ttacccgtt accgcaccc ggctgcccg gacgagcga tccagggtg gctttggct ccatcaaat aggccctaa aatgaccgg ttttcgcac aattcagcg cgcggtttt gatattaca agcaatttt tcgacgagg gactagaag caatctcaa atggtaaat ggagtaagc gttaaaaat gaatttgaa ttggttcgc acatattat cgtccatca cccctacat cattatatc ggcagttgc tgtgttctc tggtacatg cctggtgat accttttac actgaaaac caagccctt caaagactt ttcggaccc caaaatggg cttcactac cagaggaac agctatatc aaacagact aactgcttg gaagaactt agatgaatc aactcgacc atcaggaca atttgctat taaccccaa agatatttt gataaaacg aaatgtaac attttttaa acctaaagt taagaaaaa tggaaaaaa gaagaaaaa caaacaaaa aaacgattt ttctaatgt aactttgcc gctcaagtc gcccccttc taaaccacc atttcaggt atttataaa tgaagcaag taataatcg ctctgacaa tagctacga aaataataa ctggagggc tctacaaaa catgttgca cccaaaagc tgctgaaaa cgtcgccgt caaaccggt cgaaatcga cggtttttt ttgtggtta tcctgacga acgattcgc tcacatagt attaaaatc gaaaaacgc cgaggacct gtttacttt aaaatcttt actggctct gtgtcgcat agagcacga atttattga agctcacta ttacaaaga cttgtgcat ttcgaatca ggattacca cgtaaagaa gtgctgcaa gccatggtc tttagccaa ttacatagc aattttaca caatagaac ttcgtgttt agcaattac ggaagaaga cgttaacgc aacaaaaac attattgat aaattgttc gcaatattt tcataaact gcaactaat gcaagattg cttcgataa gggatactg tgatgtacc gcttgaatg cgttcgaca cgatgccgt ttttaatgt acttaattt tttaacaag cacgacgtt tctgccgcc taataacac tcgggagag tacaacgtt agaagaaac aatgatctt tctaccact agcatgtag tacagcaat tttaccata ttatcacga gattattca aatggactt agaatatga aggcatatc agttgtagc aatggaatt tgggataaa agatgctct gtttgacga gtgcctgtt acgggacgc ttggttgat gacttccgg ggcactctt tgtgttatt atgcatgtg gctatatac ggaatcatt gttcctgac tatcatgac aataattgc aattatatt ctcactggg gatttcgta ttttatgta catgctcgt ttttgagct gaaattttt tccgtttat gaatttgct agctattat tgttgcaat cggtattgg agcggacga tgcttttat attttgtaa aatatggca caaagttaa agaagaaaa aaatagctc gatagtcaa actaatggg tgatacctt ctatcatgc atttttttc gatgtttgt tacgtcctt aacgacctc ggtggcttt cctggcctc ctatgccag ctctgtgac ggcaatatg ttgtttcag catattttc ggggacggc cgtaataac gaatttttt tatgatgat cacttggtt ccccgcatg tgttgtaat atgggagcg ctcatgttg ttcaaatgt tgatttagc gaaaagttg tttaatggc gtgcttgca acgttggtg ttgttcaaa actgataaa accgacttg gaacttatc aacgacatg gccgaaatg ttcattttt ggggagaat ctggagttc caaagagcg tttcttgtt agacagtgt tgtcaattt cagatatat atggttggt tatattgtc cataatagg tttaataag tacgatcgt tgtgattta ttatccgaa atttcaact acctgactc atccgaatt ccaactgtt cgacagctc gcacccctt cgaacaata cgattttaa atacaaata tcacttttg gttcagacg cgaggaaag ggagaattt ggttggcgt taattacaa gcttccgtt acgttttgt ctggggtgt tttacccgt ggacaatgg cgaccatct gaacccgga gagttacgg caaattgga gctggatcc ggacttcga tatatctca accggaatc tcaagtgtg gctcttgaa gttttgcaa gcgcttgca acgccagcc ctttttcca acccacctt tgggcccct tttgccaaa ctgtttcat tgaaacgtt catgcgctc gatgcagag ggattgtgt tgactcgtt ttctcaaag agattggtc accttgttg caaaatgtc cacgtttcc gtataacag tagcgtgtt ttatgaatg catagtgga agagatgac ggatattta cgaaacacc tagtcaata tttgatacc tggaatggc cggtccgaa attttctaa agaccaatt tccgacaat aaaagctgt tatcgtaga gtatgatag taattatag ttattcaat gtcttatga atacatgca tgaatttta cacgaaagt cgagaactg gatgattga ggagctgaa gacggctcc caagacaat gaaaaacgg ctggttcat cagcgagtt ggagttcta cgacttgca gagagagtt atcggaaaa tactatatt agcaattat agtgtcaat gggattagc tttaatagt cttgcttct atcaacatt gaatatttt gacaagttt atacgcgat tttaactat aacgtgcag tatttttgt aactgttgc agtactagt tctgttagg gtggaagtt gaatatttt ggagtctat aactgtctc gacagctat aggtcttac tgtagattt tagtttaca ttacactgt gaattatag attatgtcc acttgctct ggcagacga gcggatcgc tgctaccaa gtacgcatt gtcttacat ggcaggtcc tgtattgat ggcagcatt aacaaccgg ggcggctgg agcattcat gatgccctc actaatctt gccatacat acagatcgg aatattcct tgtaactgt aatgagtgt tagttggat atatgccac attccatct gggtgctat gttggccat tattggacc ccagaatca gttcggcca attccacta ttccaagct tagttgttg tccttccag taaatcaaa aacgtcctc tagcgaccg aaatcgtcc tgttaatca ctcctctct ctcagatac acacgaatt agattcgtt gacttgcaa gagtggagt acggcctat tccgagacc cctccaaag gtctctcag tactggcgg ggctggcaa aaacaacca caatagata tgtttttac cgatcaatc accctccgc caccagcgc cattactat tatcatggc cgatgacaa ttaaatgta acattagga ctaaatgtt ctgtgatat actctgtat ttcttttat ttttatttc gatttttac ataaatgct ctttcaaca actatcatt tacccttgt tcaaatggc atttaagta acaaacgta cacatatat aaattaaat atatattat agaaaggac cacgctctt tcagtgcac ccataccat ccccctcct ccaattttt aagcaaacg aaagaattt tgacgatca attaataaa acgttcttt aaatt
The data is licensed under a
Creative Commons Attribution 3.0 Unported License. (CC-BY)
