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Gene information
General information
TC032224
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001247
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13204901..13205997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC032224
      iBeetle screen iB_10581

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      antenna structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      labrum size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      labrum not present -

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC032224 RA
        MSNIRIYFF LSSLLVARA TVNICIPLH SSSAEVSTP 
        DDLFKEIKD EIVAKTSNK SNLHLEHLD VDVSHPAIS 
        LYGTMDDLD LYHFHEFDI CFLENLFLN LSYYQFDVF 
        NLTFRNLRV EGKYNLTGD IGDLFDIYG VGDFWMELR 
        NFSIAMTNF SINVTDEIC IPVNIDINL QEVRNQFDN 
        FMEDPDLEI LLNGTMMNI LPESFSIFW AEMKAIFDK 
        PLEDEINNI IHHNVPAIL HALRSAQNV KAAPYCYST 
        YK
      CDS sequence
      • >TC032224 RA
        atgtccaat attaggatt tactttttt ctgagttcg 
        ctgcttgtt gctcgagcc actgtcaac atttgtatt 
        ccgctacac tcttcttct gcagaagtt tcaaccccc 
        gatgactta tttaaagaa ataaaagac gaaattgtg 
        gctaaaacc tcgaacaag tccaacttg caccttgag 
        catttggac gtagatgtt agccatccg gcaatcagc 
        ttgtacgga actatggac gatttagac ttgtaccat 
        tttcacgaa tttgatatt tgtttcctt gaaaatctt 
        ttcctgaat ctgagttat taccaattt gatgttttt 
        aatctaacg ttcagaaat ttgagagtc gaaggcaaa 
        tacaaccta actggagat attggagac ttgtttgac 
        atatacgga gtcggtgat ttctggatg gaactgcgg 
        aacttttcc atcgcaatg accaacttt agcataaac 
        gtaaccgac gaaatctgc attccggtc aatatcgat 
        attaatctc caggaagtc agaaaccaa tttgataat 
        tttatggag gaccctgac ttggagata ttactcaac 
        ggcacaatg atgaatatt ttaccagag tcatttagt 
        attttctgg gccgaaatg aaggccatt ttcgacaaa 
        cccctggaa gatgaaatt aataatata atccatcat 
        aatgttccg gcaatactg catgcatta aggagcgct 
        caaaacgta aaagcagcg ccctattgt tattcaact 
        tacaaataa
      mRNA sequence
      • >TC032224 RA
        tattagcaa aatgaaatt ttaaacatg tccaatatt 
        aggatttac ttttttctg agttcgctg cttgttgct 
        cgagccact gtcaacatt tgtattccg ctacactct 
        tcttctgca gaagtttca acccccgat gacttattt 
        aaagaaata aaagacgaa attgtggct aaaacctcg 
        aacaagtcc aacttgcac cttgagcat ttggacgta 
        gatgttagc catccggca atcagcttg tacggaact 
        atggacgat ttagacttg taccatttt cacgaattt 
        gatatttgt ttccttgaa aatcttttc ctgaatctg 
        agttattac caatttgat gtttttaat ctaacgttc 
        agaaatttg agagtcgaa ggcaaatac aacctaact 
        ggagatatt ggagacttg tttgacata tacggagtc 
        ggtgatttc tggatggaa ctgcggaac ttttccatc 
        gcaatgacc aactttagc ataaacgta accgacgaa 
        atctgcatt ccggtcaat atcgatatt aatctccag 
        gaagtcaga aaccaattt gataatttt atggaggac 
        cctgacttg gagatatta ctcaacggc acaatgatg 
        aatatttta ccagagtca tttagtatt ttctgggcc 
        gaaatgaag gccattttc gacaaaccc ctggaagat 
        gaaattaat aatataatc catcataat gttccggca 
        atactgcat gcattaagg agcgctcaa aacgtaaaa 
        gcagcgccc tattgttat tcaacttac aaataaaac 
        acctggcag ggtaaaaaa attctcacc ttgattagg 
        aaatcaatt tttatttca tgtatagaa accaaatgt 
        acaaaaata ttaaactgt atcacactt ttctgagta 
        gcacgtaca acgcctaat tagctcttt cacaaaaca 
        atacactgt gtcacacac t
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY