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Gene information
General information
TC031263
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002184
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 623385..625208
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031263
    iBeetle screen iB_10580

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031263 RA
      MRSILWCTL VNSLCVIGL MPRSDPENV KSLYTCVDN 
      ILNTVNRKK IIYAVNLTN QIKHPIINI NPTKQFTLW 
      FQYEKSDVC IINLTEGNL HLTLIKLFK NPNINPRGV 
      FLVIVSDVD RALFVAASI FYITNLIVI ETNTGTDIK 
      LYGYKPYLQ QNVNNPLTS FYNVATCRN GTLNHAISW 
      SHDAPSLWK NTTINILNN VSPPYTICH NCTKDRGIE 
      FDIFDIIVE KLQFVPNFT RNGEFVVWG DKINGTYDS 
      ILGDLEMRK GEMVIGAFH LKSEEHIDF DMTFTHMED 
      ATYWLVPKA RPLPLWKRL NLIFSLEVY FLLLISIIT 
      TMIVCHVLY KQSFSSALV MLYQILLEC SLNRMPTFK 
      TLIITWIGF CLVVSTVFK SKLMNVMTE NIYEHQIES 
      LSEIVRSNL MITIDHFIA TFYYHHTNP DEEFVVKNY 
      VYCGDHFGC VRRTAFEQD TVTVDFARS YQYTALFMD 
      SEGNLLLHL CKTPIFPVQ MHLFFVKGY PLFPQIDEL 
      LIRIKSSGF IQYHYNKVA YYTQIALNR KKHNLAAQV 
      LSVKHLQIA FVIFGIGIL LSFVAFLAE YFINKLSVS 
      V
    CDS sequence
    • >TC031263 RA
      atgcgttcg attttgtgg tgtacctta gttaatagt 
      ttatgtgtg attggttta atgccacgg tcagaccct 
      gaaaatgta aaatctctc tacacttgt gttgacaat 
      attttaaat acagtgaac agaaagaaa attatttac 
      gccgttaat ttaacaaac cagataaaa catcccatc 
      attaatatt aatccaacc aaacaattc acattatgg 
      tttcagtat gaaaagagc gatgtttgt atcattaat 
      ttgactgaa ggcaatttg catttgact cttattaag 
      ttgtttaag aaccccaac attaatcct cgtggagtg 
      tttcttgtt atagttagt gatgtggat cgggcttta 
      tttgttgca gcttcaatt ttctacatt actaattta 
      atcgttatt gagacgaat acgggaact gatattaaa 
      ctctatggt tataaacct tatttgcaa caaaatgtt 
      aataaccct ttaactagt ttttataat gttgcgacc 
      tgtcgaaat ggcactcta aaccatgcc attagctgg 
      tcacatgac gcaccaagt ctttggaaa aacacaaca 
      attaatatt ttgaataat gtttcacca ccatacacc 
      atatgccat aattgtacg aaagatcga ggaatagaa 
      tttgatatt tttgatata atcgtcgaa aaattacaa 
      ttcgtcccg aattttact agaaatggt gaatttgta 
      gtttggggt gacaaaatc aacggaact tatgattca 
      attttgggc gatttagag atgcgaaaa ggggaaatg 
      gtgattgga gcttttcac ttgaaatcg gaagaacac 
      attgatttt gatatgact tttacacac atggaagat 
      gccacgtat tggttggta ccgaaagca agacctttg 
      cctttgtgg aaacgactg aatttaatt ttcagttta 
      gaagtttat ttcttgcta ctaatttcc ataataacg 
      acaatgata gtttgccat gttttgtat aaacaatcc 
      ttctcgtct gccttagtt atgctctac caaatttta 
      ctagaatgt agcttgaat cgaatgcca acatttaaa 
      acacttatt ataacatgg attggattt tgccttgta 
      gtttcaaca gtatttaaa agcaaatta atgaatgtg 
      atgaccgaa aatatctac gaacaccaa attgaaagc 
      ttaagtgaa attgttaga tcaaattta atgataact 
      attgaccac ttcattgca acgttttat tatcatcac 
      acaaaccct gacgaagaa tttgtagtc aaaaattat 
      gtttattgt ggagaccac tttggttgt gtgagaaga 
      acagctttt gaacaagat actgtgaca gttgatttc 
      gcaagaagt tatcaatac acagctttg ttcatggat 
      tctgaagga aatctcctt ttgcattta tgcaaaact 
      ccgattttt cctgtgcag atgcatttg tttttcgta 
      aaaggttat ccgcttttt ccacagatt gatgagctg 
      ctgattaga attaagtct agcggtttt atacaatat 
      cattacaat aaagtggct tactataca caaatagca 
      ctaaatagg aaaaagcat aatctggca gcacaagtt 
      ctcagtgta aaacattta caaattgca tttgtcatc 
      tttggtatt ggaatttta ctatcgttt gtggcattt 
      cttgcagaa tattttatc aataaatta tcagttagt 
      gtctaa
    mRNA sequence
    • >TC031263 RA
      ctcttcttg ttgcaacaa aatgcgttc gattttgtg 
      gtgtacctt agttaatag tttatgtgt gattggttt 
      aatgccacg gtcagaccc tgaaaatgt aaaatctct 
      ctacacttg tgttgacaa tattttaaa tacagtgaa 
      cagaaagaa aattattta cgccgttaa tttaacaaa 
      ccagataaa acatcccat cattaatat taatccaac 
      caaacaatt cacattatg gtttcagta tgaaaagag 
      cgatgtttg tatcattaa tttgactga aggcaattt 
      gcatttgac tcttattaa gttgtttaa gaaccccaa 
      cattaatcc tcgtggagt gtttcttgt tatagttag 
      tgatgtgga tcgggcttt atttgttgc agcttcaat 
      tttctacat tactaattt aatcgttat tgagacgaa 
      tacgggaac tgatattaa actctatgg ttataaacc 
      ttatttgca acaaaatgt taataaccc tttaactag 
      tttttataa tgttgcgac ctgtcgaaa tggcactct 
      aaaccatgc cattagctg gtcacatga cgcaccaag 
      tctttggaa aaacacaac aattaatat tttgaataa 
      tgtttcacc accatacac catatgcca taattgtac 
      gaaagatcg aggaataga atttgatat ttttgatat 
      aatcgtcga aaaattaca attcgtccc gaattttac 
      tagaaatgg tgaatttgt agtttgggg tgacaaaat 
      caacggaac ttatgattc aattttggg cgatttaga 
      gatgcgaaa aggggaaat ggtgattgg agcttttca 
      cttgaaatc ggaagaaca cattgattt tgatatgac 
      ttttacaca catggaaga tgccacgta ttggttggt 
      accgaaagc aagaccttt gcctttgtg gaaacgact 
      gaatttaat tttcagttt agaagttta tttcttgct 
      actaatttc cataataac gacaatgat agtttgcca 
      tgttttgta taaacaatc cttctcgtc tgccttagt 
      tatgctcta ccaaatttt actagaatg tagcttgaa 
      tcgaatgcc aacatttaa aacacttat tataacatg 
      gattggatt ttgccttgt agtttcaac agtatttaa 
      aagcaaatt aatgaatgt gatgaccga aaatatcta 
      cgaacacca aattgaaag cttaagtga aattgttag 
      atcaaattt aatgataac tattgacca cttcattgc 
      aacgtttta ttatcatca cacaaaccc tgacgaaga 
      atttgtagt caaaaatta tgtttattg tggagacca 
      ctttggttg tgtgagaag aacagcttt tgaacaaga 
      tactgtgac agttgattt cgcaagaag ttatcaata 
      cacagcttt gttcatgga ttctgaagg aaatctcct 
      tttgcattt atgcaaaac tccgatttt tcctgtgca 
      gatgcattt gtttttcgt aaaaggtta tccgctttt 
      tccacagat tgatgagct gctgattag aattaagtc 
      tagcggttt tatacaata tcattacaa taaagtggc 
      ttactatac acaaatagc actaaatag gaaaaagca 
      taatctggc agcacaagt tctcagtgt aaaacattt 
      acaaattgc atttgtcat ctttggtat tggaatttt 
      actatcgtt tgtggcatt tcttgcaga atattttat 
      caataaatt atcagttag tgtctaaat ttattgttt 
      cttttcgac gcgaaatct tattaggtc taaacgagt 
      ttgaattaa aattccaag atttca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY