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Gene information
General information
TC011520 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3753856..3756090
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011520
    iBeetle screen iB_10579

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011520 RA
      MEESSDAHG YSKLGKSRR RNDFHNSNT FSNISPPID 
      SYNAVYLSF VLGGAGFLL PYNSFIMAM DYFKVRYPD 
      TPVVFDMSL VYIAVAFLT VLGNNLLVE TFTLNSRIN 
      FGYIMSFIT LIFVVVCEV WWEAFGTAT SYGVNLAAV 
      AVVAVGCTV QQSSFYGYT SMLPARYTQ AVMVGESAS 
      GVFTSSVRV LTRFLIPEI RGSTIYFFT VSVSAVATC 
      FAMYHLIRR TDFIQFYIA LCERAKTRI TLEPSEDAG 
      LIETNDAQY GVLKIQNSP PPGNTLSFA NPAYEPSAP 
      VPVPTYKVE DVVIRGRQS VSTAGGLSG MKRGLLARW 
      EITKAIHPY MISICLVYF ATLCLYPGI ASEIISCRL 
      GSWMPILMM ALFNGADLF GKMLASSSR YWTGGRLVR 
      CSVARLIMI PLMIMCVAP RANPIFSAE VTAFTFALI 
      LGFSNGILG SVPMIQAPS KVDDRYREL TGNMMTLLY 
      NFGLTTGSL MAYFLESML NPMDNHPCS LNHLHMKMT 
      ESPVNISFT TGTIRSTSV TIITLASTL STGADNSTD 
      FSR
    CDS sequence
    • >TC011520 RA
      atggaggag tccagcgat gcgcacggt tattccaaa 
      ttgggaaaa tctcgtcgg cggaatgat ttccacaat 
      agtaacacg ttttcaaat atatcaccc cccatagat 
      tcgtacaat gcggtctat ttgtcgttt gtcctcggt 
      ggtgctggt tttctgctc ccttacaat agtttcata 
      atggcaatg gattatttc aaagtcagg tatcccgac 
      actcctgtt gttttcgac atgtctttg gtgtacatc 
      gctgttgct tttttgacc gttctgggc aataatttg 
      ctggtcgag actttcacc ctcaattcc agaatcaat 
      ttcggatat atcatgtct tttatcact ttgatattt 
      gttgttgtt tgcgaagtg tggtgggaa gctttcggc 
      actgccact tcttacggt gttaacttg gcagctgtg 
      gcggttgtg gcagtcggc tgcacagta caacagtca 
      agtttttac ggatataca agtatgctg ccagcgaga 
      tacacccaa gctgtgatg gtaggtgaa agcgcttct 
      ggggtcttt acgtcatca gtgcgggtc cttaccaga 
      tttttgata ccagaaatc cggggaagt acaatatac 
      tttttcacc gtgtcagtt tcagctgta gctacatgc 
      ttcgccatg taccacctt atcagaaga actgatttt 
      atacaattc tacatagct ttatgcgag agggctaag 
      actaggatc acgcttgag ccgagcgaa gacgcagga 
      ctgatcgag accaatgac gcccagtat ggggttttg 
      aagatccag aacagcccc ccgccgggg aacaccctc 
      agttttgcc aacccggct tacgagccg tcggctccc 
      gtccctgtg cccacgtac aaagtggaa gatgtggtg 
      attagaggt aggcaaagt gtgagtaca gctggagga 
      ctcagcggc atgaaaagg ggtttactg gcgcggtgg 
      gaaatcacg aaagcgatc catccgtac atgatatcg 
      atttgcttg gtttatttc gccactctg tgtctgtac 
      cctgggata gcttcggaa attatcagt tgtcggttg 
      ggttcgtgg atgcccatt ctgatgatg gcgttgttc 
      aatggggcc gatttgttc gggaaaatg ttggcgtct 
      tcgtccaga tactggact ggtggtaga ttggttcgc 
      tgttcggtc gctcgactc ataatgatt cctttaatg 
      atcatgtgt gtggctccg agggcaaat ccgattttt 
      tcggctgag gtgactgcc ttcaccttt gcgttgatt 
      ttgggtttc agtaatggg atcttgggg agcgttccc 
      atgattcag gcgccgagt aaagtggac gatcgctat 
      agggaactc actggcaat atgatgact ttgttgtat 
      aattttgga ctaactaca ggatcttta atggcgtat 
      tttttggaa tcgatgttg aaccctatg gacaaccat 
      ccgtgcagc ctcaatcat ctccacatg aagatgacg 
      gagtctccc gtcaatatt agctttacg actggtacc 
      atccgcagt acttcggtt acaatcatt actctggca 
      tctacttta tcaactggt gctgacaac tccacagat 
      tttagtcga taa
    mRNA sequence
    • >TC011520 RA
      ctcgatccg caagcgcaa ttttgtaat caatagacc 
      tagaaacat gcgcaagat atttctgtt gtaaaaatg 
      taagtgtaa taattttcc aatatttat tttatctaa 
      aattataat aataacacg tgtgagtga aatcggcgt 
      ctaaatatg cataaaagc aattaatcg ggcgtttcg 
      ttcgaaatg gaggagtcc agcgatgcg cacggttat 
      tccaaattg ggaaaatct cgtcggcgg aatgatttc 
      cacaatagt aacacgttt tcaaatata tcacccccc 
      atagattcg tacaatgcg gtctatttg tcgtttgtc 
      ctcggtggt gctggtttt ctgctccct tacaatagt 
      ttcataatg gcaatggat tatttcaaa gtcaggtat 
      cccgacact cctgttgtt ttcgacatg tctttggtg 
      tacatcgct gttgctttt ttgaccgtt ctgggcaat 
      aatttgctg gtcgagact ttcaccctc aattccaga 
      atcaatttc ggatatatc atgtctttt atcactttg 
      atatttgtt gttgtttgc gaagtgtgg tgggaagct 
      ttcggcact gccacttct tacggtgtt aacttggca 
      gctgtggcg gttgtggca gtcggctgc acagtacaa 
      cagtcaagt ttttacgga tatacaagt atgctgcca 
      gcgagatac acccaagct gtgatggta ggtgaaagc 
      gcttctggg gtctttacg tcatcagtg cgggtcctt 
      accagattt ttgatacca gaaatccgg ggaagtaca 
      atatacttt ttcaccgtg tcagtttca gctgtagct 
      acatgcttc gccatgtac caccttatc agaagaact 
      gattttata caattctac atagcttta tgcgagagg 
      gctaagact aggatcacg cttgagccg agcgaagac 
      gcaggactg atcgagacc aatgacgcc cagtatggg 
      gttttgaag atccagaac agccccccg ccggggaac 
      accctcagt tttgccaac ccggcttac gagccgtcg 
      gctcccgtc cctgtgccc acgtacaaa gtggaagat 
      gtggtgatt agaggtagg caaagtgtg agtacagct 
      ggaggactc agcggcatg aaaaggggt ttactggcg 
      cggtgggaa atcacgaaa gcgatccat ccgtacatg 
      atatcgatt tgcttggtt tatttcgcc actctgtgt 
      ctgtaccct gggatagct tcggaaatt atcagttgt 
      cggttgggt tcgtggatg cccattctg atgatggcg 
      ttgttcaat ggggccgat ttgttcggg aaaatgttg 
      gcgtcttcg tccagatac tggactggt ggtagattg 
      gttcgctgt tcggtcgct cgactcata atgattcct 
      ttaatgatc atgtgtgtg gctccgagg gcaaatccg 
      attttttcg gctgaggtg actgccttc acctttgcg 
      ttgattttg ggtttcagt aatgggatc ttggggagc 
      gttcccatg attcaggcg ccgagtaaa gtggacgat 
      cgctatagg gaactcact ggcaatatg atgactttg 
      ttgtataat tttggacta actacagga tctttaatg 
      gcgtatttt ttggaatcg atgttgaac cctatggac 
      aaccatccg tgcagcctc aatcatctc cacatgaag 
      atgacggag tctcccgtc aatattagc tttacgact 
      ggtaccatc cgcagtact tcggttaca atcattact 
      ctggcatct actttatca actggtgct gacaactcc 
      acagatttt agtcgataa attatagga acgaatgta 
      tctattctg gctttgtta gacattacc attattaaa 
      aacattaat aaaaaatca tatcgatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY