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Gene information
General information
TC011828 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 13056453..13057647
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011828
      iBeetle screen iB_10577

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      larva dorsal bent - Penetrance (percentage of animals/eggs on slide): less than 30%

      larva size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head appendages random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      labium potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

      maxilla potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
      labrum size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      labrum position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      posterior terminus not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011828 RA
        MLRLIVVTL CVSAATCGD SKCANGDFQ 
        CELVEFLDE LDREPAVDV LGLTLVRKS 
        GHFRARTDP GLIERFVQF VESHEVKIA 
        PSITDRNWE NEARTKKLK KILFPILMF 
        LKLKAAIVI PAVLALMGA LAVKGFGMS 
        LVALTVAVG VALKNFVAE EHYHHGPKV 
        AYEIVPQVW QRNAMEQGA PLAYQPIS
      CDS sequence
      • >TC011828 RA
        atgctgcgt ttgattgtc gttactttg tgcgtttct 
        gcggcgact tgcggtgat tcaaagtgc gctaacggt 
        gatttccag tgcgaattg gtcgaattt ttggacgag 
        ctggaccgc gagccggcc gtcgacgtc ctcggcctg 
        actctggtg cgaaaatcc ggtcatttc cgcgcgcga 
        accgatccc ggattaata gaacgtttc gtccaattt 
        gtggaaagc catgaagtc aaaatcgct ccatccata 
        acggaccgg aactgggag aatgaagcg aggacgaaa 
        aagctgaaa aaaatcctg ttcccgatt ttgatgttc 
        ctgaaactg aaagcagcg attgtgatt ccggcggtt 
        ctggccctg atgggggcc cttgctgtt aagggattc 
        ggcatgagt ttggtggcg ctgacggtg gctgttggg 
        gtggctctg aagaatttt gtggccgag gagcactac 
        caccatggg cctaaagtg gcctacgag attgtgccg 
        caggtgtgg cagaggaat gccatggaa cagggggcc 
        ccgttggcc taccaaccg atctcttaa
      mRNA sequence
      • >TC011828 RA
        aatggctga ttgtgtccc aagacacgc attaacacg 
        gaatttttc tttatccgt gttacatat tttacgcaa 
        acccctact atcattatc accacataa atagtcaat 
        taccacccg caagatcgc ccaaaaccc gaaaccaac 
        gtcgaaaaa atgctgcgt ttgattgtc gttactttg 
        tgcgtttct gcggcgact tgcggtgat tcaaagtgc 
        gctaacggt gatttccag tgcgaattg gtcgaattt 
        ttggacgag ctggaccgc gagccggcc gtcgacgtc 
        ctcggcctg actctggtg cgaaaatcc ggtcatttc 
        cgcgcgcga accgatccc ggattaata gaacgtttc 
        gtccaattt gtggaaagc catgaagtc aaaatcgct 
        ccatccata acggaccgg aactgggag aatgaagcg 
        aggacgaaa aagctgaaa aaaatcctg ttcccgatt 
        ttgatgttc ctgaaactg aaagcagcg attgtgatt 
        ccggcggtt ctggccctg atgggggcc cttgctgtt 
        aagggattc ggcatgagt ttggtggcg ctgacggtg 
        gctgttggg gtggctctg aagaatttt gtggccgag 
        gagcactac caccatggg cctaaagtg gcctacgag 
        attgtgccg caggtgtgg cagaggaat gccatggaa 
        cagggggcc ccgttggcc taccaaccg atctcttaa 
        tgtgaacgc ttctttatt tattggttt taataaaat 
        atttaaaca agt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY