Status
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Gene information
General information
TC033610
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015930
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4201665..4206758
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033610
    iBeetle screen iB_10576

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033610 RA
      MGKHWCYFL FMLWFSYLI VSSILLFSR GFLLSKNAQ 
      TTNSTCLSL SEIPCIHKE STTLSAHEQ QCSADTKLS 
      YVFQNINSA SDICLPQRA RVVFVIIDA LRYDFALYD 
      ENLKNPLPF QNKLPVINE LLKQQPDNS RLYKFIADP 
      PTTTMQRLK ALTTGSLPT FIDAGSNFA TNEINEDNI 
      IDQLLRHNL STVLIGDDT WDGLYPNRF LRKYPYPSF 
      DVWDLDTVD DGVNFHLYP ELAKNDWSF LIAHYLGVD 
      HCGHRYGPN HSEMERKLT EMNTVIASI VERLDPSTM 
      LFVIGDHGM TETGNHGGD ADDEVTSAL FVYSHTQLS 
      SLHASATVR QVSLVPTLA SIFGVSIPF SNLGTVILD 
      ALPLLNNSQ VPEWQWSLF HLWANAQQM LTYIDHYAK 
      SAPETFSDS TLQSLRENY ALLNSKLFH VSDQPSFAT 
      FTEECLVFL GKLRETCEQ VWVQFDSFS MTRGLLFLF 
      LSMFFVYVI TDGIPSNRL PEIFMSSFL TCSYFVLLV 
      AVCVSIVLY HFSYVSSLT STIFFSTGL VSQFMLGML 
      VIQNWELIS LNWYDRSKK ERIPNLICR LVLVFHLCG 
      LFSNSFVVE ESSVLLFLL TTVILIGSL SVGQGETKK 
      KIPRPKLRL LLIAFGMLV LVRISMYFW RCRDRLEQP 
      WCYETYNSF TSKAETTKL QWITTLITL ALFVTTTKV 
      WLRNCGNLN GYNFTVIVL KYAPTVLVV CTSGFWVLN 
      RLPGDPKNK AIRPWQANV LAWTVYGVS FVGILVVVL 
      KPLCVYVLP KEKTVEGQN IIPHLFHKV KRLFNEESR 
      DDVPILCGL GTAYSAVFV SIGIFLTHL FALIHGDGV 
      APATVIMFL ASAFVLIIT SIIRLEKAT IMEQLFEIP 
      NMSLLVWVI LSQYFFYGT GHQPSFPNI IWEAAFVGT 
      SGVISHNYI LGSLIILNT FCSQILMGF LVPLLIIMP 
      FTVSVMLPT SINRNILER ENLRGEVLL YERDGLMIT 
      VAFTTICKY MVCHGIRVF ATMLAATIH CRHMMIWKI 
      FAPKLIFEA IGMFVTLVV VNLSYLVLL RINFKVDQL 
      IVSLNKRCR
    CDS sequence
    • >TC033610 RA
      atgggcaaa cactggtgc tatttcttg ttcatgcta 
      tggttctcg tacttgata gtctcgtca attttgctc 
      ttttcgcgc ggattcctc ctctccaaa aatgcccaa 
      acaaccaac tccacttgc ctctccctg tccgagatc 
      ccgtgcatt cacaaggaa tcgacgact ttaagtgcc 
      cacgaacag caatgcagt gccgacacc aaactatcc 
      tatgttttt caaaacata aacagcgct tcggatatt 
      tgcttgccc caaagagcg cgcgttgtc ttcgtgata 
      atcgacgct ctgcggtac gactttgca ctctatgac 
      gagaattta aaaaaccct ctgccgttc caaaacaag 
      ctgccggtt ataaacgag ttgctgaag caacaaccg 
      gacaattcg cggctgtat aagttcata gcggacccc 
      ccgaccacc acaatgcaa agactgaaa gctctaacg 
      acgggaagt cttccaact tttatcgac gccggctcc 
      aattttgcc acgaatgaa atcaacgag gataatatc 
      attgaccaa ttgttgagg cacaacttg agcacggtc 
      ttgatcggc gacgacact tgggacggc ctctaccct 
      aacagattt ctcaggaaa tacccctat ccctctttc 
      gatgtctgg gatttggac acagttgac gacggagtc 
      aatttccac ctttacccc gaattggca aaaaatgat 
      tggagtttt ctgatcgca cattacttg ggggtggac 
      cactgtggc catcgctac ggccccaac cattcggag 
      atggagcgc aaattgaca gaaatgaac accgtgatt 
      gcgagcatt gtggagcga ctagacccc agcacaatg 
      ctgttcgtg atcggcgac cacggaatg accgaaacc 
      ggcaaccac gggggcgac gccgacgac gaagtcacc 
      tcagccctc ttcgtgtac tcccacacc caactctcc 
      tccctccac gcctccgcc accgtccgc caagtcagc 
      cttgtccca acccttgcc tcaattttc ggcgtttcg 
      atcccgttc tcaaacctc ggaacggta atcctggac 
      gcccttccg ctcctcaac aactcacaa gtgcccgaa 
      tggcaatgg tccctcttc cacctatgg gcaaatgcc 
      caacaaatg ctcacctac atcgaccac tacgccaaa 
      tcggccccg gaaaccttc agcgactca actttgcaa 
      agcctgcgc gagaactac gccctcctg aacagcaaa 
      ctcttccac gtcagtgac caaccctcc tttgcgact 
      ttcacagag gagtgtttg gtttttttg gggaaattg 
      cgcgaaact tgcgagcaa gtctgggtc cagttcgat 
      tcgttttcg atgactcgt ggattgttg ttcctgttc 
      ctgtccatg tttttcgtc tatgtgatc actgacggg 
      attcccagc aaccgcctc ccggagatt ttcatgagt 
      tcgttcctc acttgttct tattttgtt ttgttagtt 
      gcggtttgc gtctcgatt gttttgtac cacttttcc 
      tacgtttca agtctcaca tccacgatt tttttctca 
      accggactg gtctcccag ttcatgcta ggcatgctg 
      gtgattcaa aactgggag ttgatcagt ctcaactgg 
      tacgatcgg agcaagaaa gaacgtatc ccgaatcta 
      atctgccgg ctggtgctc gttttccac ttgtgtggc 
      cttttttcc aacagtttt gtcgttgag gagtcgtcc 
      gttttgctg tttctccta accacggtt attttgatt 
      gggagtttg agcgtcggt cagggagaa acaaagaaa 
      aaaatcccg agaccgaag ttgcgattg ttgttaatt 
      gcgttcggg atgttggtg cttgtgcgc atttccatg 
      tatttctgg cgctgtcgc gaccgttta gagcaaccc 
      tggtgttac gaaacgtac aattcgttc acctctaaa 
      gccgaaacg accaaactc cagtggatc accactctc 
      ataaccttg gccctgttt gtcacaacc accaaagtt 
      tggctgcga aattgtggc aacttgaac ggttacaac 
      ttcacggtg attgtgcta aagtacgcg cccacggtt 
      ctggtcgtg tgcacgagc ggtttctgg gtcttgaac 
      cggctcccg ggtgatccg aagaacaag gcgatccgg 
      ccctggcag gcgaatgtt ttggcttgg actgtttat 
      ggtgtttcg tttgttggg attttggtc gtggttttg 
      aagccattg tgcgtttac gttttaccg aaggaaaag 
      acagtcgag gggcagaat atcatcccg catttgttc 
      cacaaagtg aagaggctc ttcaatgaa gaaagccgg 
      gatgatgtc ccgattttg tgcgggctg gggactgcc 
      tacagtgcc gtttttgtc tcgattggg atttttctg 
      acgcatttg ttcgcattg attcatggg gatggtgtt 
      gccccggct actgttatc atgtttctg gcgtcggcg 
      tttgttctg attattacg tcgattatc cggctcgag 
      aaagccaca attatggaa caactgttc gaaattccg 
      aacatgtcc ctcctggtg tgggtgatc ctctcccag 
      tatttcttc tacggaacg ggtcaccag ccctccttc 
      cccaacata atctgggag gcggccttc gtggggact 
      tcgggcgta atcagccac aactacatc ctcggcagt 
      ttgataatt ctgaacacg ttttgttcg caaattttg 
      atgggattt ttggtgcct ctgctgatt ataatgccg 
      tttacggtc tccgtcatg ttaccaact tccattaat 
      cggaatatc ctcgaacgg gaaaatttg cgaggggag 
      gttttgttg tacgaacgc gatgggttg atgatcacg 
      gtcgccttc accaccatt tgcaagtac atggtgtgc 
      cacggcatt agggttttt gcgaccatg ttggcagcc 
      acgattcat tgcaggcac atgatgatt tggaagatt 
      ttcgcgccg aaattgatt tttgaagca attggcatg 
      tttgtgact ttggttgta gttaatttg agttatttg 
      gtgcttctg aggattaac tttaaagtg gatcaattg 
      attgtgtcg ctgaataaa aggtgcaga tag
    mRNA sequence
    • >TC033610 RA
      tgggagtgt gacatttca aaagttggg ttagtgata 
      aaattataa attaaacca aacgagact tttctaaat 
      gacttgtgc acaatgggc aaacactgg tgctatttc 
      ttgttcatg ctatggttc tcgtacttg atagtctcg 
      tcaattttg ctcttttcg cgcggattc ctcctctcc 
      aaaaatgcc caaacaacc aactccact tgcctctcc 
      ctgtccgag atcccgtgc attcacaag gaatcgacg 
      actttaagt gcccacgaa cagcaatgc agtgccgac 
      accaaacta tcctatgtt tttcaaaac ataaacagc 
      gcttcggat atttgcttg ccccaaaga gcgcgcgtt 
      gtcttcgtg ataatcgac gctctgcgg tacgacttt 
      gcactctat gacgagaat ttaaaaaac cctctgccg 
      ttccaaaac aagctgccg gttataaac gagttgctg 
      aagcaacaa ccggacaat tcgcggctg tataagttc 
      atagcggac cccccgacc accacaatg caaagactg 
      aaagctcta acgacggga agtcttcca acttttatc 
      gacgccggc tccaatttt gccacgaat gaaatcaac 
      gaggataat atcattgac caattgttg aggcacaac 
      ttgagcacg gtcttgatc ggcgacgac acttgggac 
      ggcctctac cctaacaga tttctcagg aaatacccc 
      tatccctct ttcgatgtc tgggatttg gacacagtt 
      gacgacgga gtcaatttc cacctttac cccgaattg 
      gcaaaaaat gattggagt tttctgatc gcacattac 
      ttgggggtg gaccactgt ggccatcgc tacggcccc 
      aaccattcg gagatggag cgcaaattg acagaaatg 
      aacaccgtg attgcgagc attgtggag cgactagac 
      cccagcaca atgctgttc gtgatcggc gaccacgga 
      atgaccgaa accggcaac cacgggggc gacgccgac 
      gacgaagtc acctcagcc ctcttcgtg tactcccac 
      acccaactc tcctccctc cacgcctcc gccaccgtc 
      cgccaagtc agccttgtc ccaaccctt gcctcaatt 
      ttcggcgtt tcgatcccg ttctcaaac ctcggaacg 
      gtaatcctg gacgccctt ccgctcctc aacaactca 
      caagtgccc gaatggcaa tggtccctc ttccaccta 
      tgggcaaat gcccaacaa atgctcacc tacatcgac 
      cactacgcc aaatcggcc ccggaaacc ttcagcgac 
      tcaactttg caaagcctg cgcgagaac tacgccctc 
      ctgaacagc aaactcttc cacgtcagt gaccaaccc 
      tcctttgcg actttcaca gaggagtgt ttggttttt 
      ttggggaaa ttgcgcgaa acttgcgag caagtctgg 
      gtccagttc gattcgttt tcgatgact cgtggattg 
      ttgttcctg ttcctgtcc atgtttttc gtctatgtg 
      atcactgac gggattccc agcaaccgc ctcccggag 
      attttcatg agttcgttc ctcacttgt tcttatttt 
      gttttgtta gttgcggtt tgcgtctcg attgttttg 
      taccacttt tcctacgtt tcaagtctc acatccacg 
      atttttttc tcaaccgga ctggtctcc cagttcatg 
      ctaggcatg ctggtgatt caaaactgg gagttgatc 
      agtctcaac tggtacgat cggagcaag aaagaacgt 
      atcccgaat ctaatctgc cggctggtg ctcgttttc 
      cacttgtgt ggccttttt tccaacagt tttgtcgtt 
      gaggagtcg tccgttttg ctgtttctc ctaaccacg 
      gttattttg attgggagt ttgagcgtc ggtcaggga 
      gaaacaaag aaaaaaatc ccgagaccg aagttgcga 
      ttgttgtta attgcgttc gggatgttg gtgcttgtg 
      cgcatttcc atgtatttc tggcgctgt cgcgaccgt 
      ttagagcaa ccctggtgt tacgaaacg tacaattcg 
      ttcacctct aaagccgaa acgaccaaa ctccagtgg 
      atcaccact ctcataacc ttggccctg tttgtcaca 
      accaccaaa gtttggctg cgaaattgt ggcaacttg 
      aacggttac aacttcacg gtgattgtg ctaaagtac 
      gcgcccacg gttctggtc gtgtgcacg agcggtttc 
      tgggtcttg aaccggctc ccgggtgat ccgaagaac 
      aaggcgatc cggccctgg caggcgaat gttttggct 
      tggactgtt tatggtgtt tcgtttgtt gggattttg 
      gtcgtggtt ttgaagcca ttgtgcgtt tacgtttta 
      ccgaaggaa aagacagtc gaggggcag aatatcatc 
      ccgcatttg ttccacaaa gtgaagagg ctcttcaat 
      gaagaaagc cgggatgat gtcccgatt ttgtgcggg 
      ctggggact gcctacagt gccgttttt gtctcgatt 
      gggattttt ctgacgcat ttgttcgca ttgattcat 
      ggggatggt gttgccccg gctactgtt atcatgttt 
      ctggcgtcg gcgtttgtt ctgattatt acgtcgatt 
      atccggctc gagaaagcc acaattatg gaacaactg 
      ttcgaaatt ccgaacatg tccctcctg gtgtgggtg 
      atcctctcc cagtatttc ttctacgga acgggtcac 
      cagccctcc ttccccaac ataatctgg gaggcggcc 
      ttcgtgggg acttcgggc gtaatcagc cacaactac 
      atcctcggc agtttgata attctgaac acgttttgt 
      tcgcaaatt ttgatggga tttttggtg cctctgctg 
      attataatg ccgtttacg gtctccgtc atgttacca 
      acttccatt aatcggaat atcctcgaa cgggaaaat 
      ttgcgaggg gaggttttg ttgtacgaa cgcgatggg 
      ttgatgatc acggtcgcc ttcaccacc atttgcaag 
      tacatggtg tgccacggc attagggtt tttgcgacc 
      atgttggca gccacgatt cattgcagg cacatgatg 
      atttggaag attttcgcg ccgaaattg atttttgaa 
      gcaattggc atgtttgtg actttggtt gtagttaat 
      ttgagttat ttggtgctt ctgaggatt aactttaaa 
      gtggatcaa ttgattgtg tcgctgaat aaaaggtgc 
      agatagtaa attattttt tttgagtgc gacattgga 
      aacaaatta aagtgcttg ttatagacg ttgtattat 
      aattttgta gccactaaa aatgtgaca acgtaatgt 
      taatgactc actcaggtt tgttatttt tactacatg 
      tagacttgt aaatagcag caataattg tcgactttt 
      ttataatac aaagtgatc aaaaaagag tgcttccgt 
      tggcaagag taatagtac tttaatttt ttgggtgtt 
      tgcaatact gctgtggtt tttccgtta ataatttga 
      cctaaacaa cttaaacag tcatttttg atcacatgg 
      cactttaat aaaaattag tagcaaata aattaaaag 
      ctttaggtt tgtgtactg ctaataatc tagtcgaaa 
      aaggcattc aaggataaa gtacttgtt tatctgctc 
      gtttttttt aggtaatac tgttcttct tgttttttt 
      gatagtatt attttgatg ttgagaatg ttttggata 
      aaaaatcct taaagctgt cgaattatt tatttatat 
      tttttgaca ataaaagtg tgtttctta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY