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Gene information
General information
TC015025 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4525874..4528103
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC015025
      iBeetle screen iB_10572

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC015025 RA
        MQTSLLILS LVVFVGAKD MMRSIVFDK 
        NTPDVFYCP IQKPTGFDK MIVKARPLK 
        KLCEFEGEP LPDDYKSDC YQDMDETKY 
        ACKEKYRIM KRLKKASEE
      • >TC015025 RB
        MQTSLLILS LVVFVGAKD MMRSIVFDK 
        NTPDVFYCP IQKPTGFDK MIVKARPLK 
        KLCEFEGEP LPDDYKSDC YQDMDETKY 
        ACKEKYRIM MRMHPPGTD EPFDGKRLA 
        RFIDVDKEF YVMKIDKKT HLKNRLG
      CDS sequence
      • >TC015025 RA
        atgcaaacg tcactcttg atcctgtct ttggttgtc 
        ttcgtgggg gccaaagac atgatgcgg agcatagtc 
        ttcgacaag aacaccccc gacgtgttt tactgcccc 
        attcagaag cccacaggt ttcgacaaa atgatagtc 
        aaggcgcgc cctttgaaa aaactgtgc gagttcgag 
        ggggagccc ctgcctgat gactacaaa agcgactgt 
        taccaggac atggacgag acgaaatac gcatgcaag 
        gaaaaatac cgaattatg aaacggctc aaaaaggct 
        tcagaggaa taa
      • >TC015025 RB
        atgcaaacg tcactcttg atcctgtct ttggttgtc 
        ttcgtgggg gccaaagac atgatgcgg agcatagtc 
        ttcgacaag aacaccccc gacgtgttt tactgcccc 
        attcagaag cccacaggt ttcgacaaa atgatagtc 
        aaggcgcgc cctttgaaa aaactgtgc gagttcgag 
        ggggagccc ctgcctgat gactacaaa agcgactgt 
        taccaggac atggacgag acgaaatac gcatgcaag 
        gaaaaatac cgaattatg atgcggatg catccgcct 
        ggcactgac gagccgttc gatggtaaa agactcgcg 
        agatttatc gatgttgat aaggaattt tacgttatg 
        aagattgat aagaagacc catttgaag aacaggctg 
        gggtga
      mRNA sequence
      • >TC015025 RA
        gactttagc actacaata atgaattaa aaaggggct 
        aaaacttta gccccgacc aatttttta gggattttt 
        cgatttggg atgacagtt gttgggggg aaccagcgt 
        aaccacttg attcaaata aaaaaataa aaaaactca 
        ccaattttt cccaattaa aaccgcaat catccaagc 
        gtagcgtat taattgtgc gaccttctg ttgattaaa 
        tttgaatgt ttatgatct aacagtgaa aaatactta 
        caaggcaac aagtcgccc aattcacca attacgata 
        attatcact aaaaccgtc cagtcttca catgactca 
        ctgtaaacc cacctgtga cttcttctt acggtttac 
        ctgacgggc acccacttt gagtcataa actcgcagt 
        cataaaatt cggacttgt ggctaaatt ttactgttt 
        gatcgagtg gcccttgat agtaaaaaa acaacaagc 
        gattggtcg aacaaattg acacgtgac cataagttt 
        ttcaaaatt gcaatttta tgactggtt tatgacaaa 
        gctcgatgt tcgcttgtc ctttcacgc gtcgcggcc 
        aacgcgatt tcttcatct ggtaagttg agggccgct 
        ttgtgtctt tatcgagtg actaagtgc gaatctagt 
        cgtcggggt gcaaagaaa ttttgaaac aacgcaaaa 
        atgcaaacg tcactcttg atcctgtct ttggttgtc 
        ttcgtgggg gccaaagac atgatgcgg agcatagtc 
        ttcgacaag aacaccccc gacgtgttt tactgcccc 
        attcagaag cccacaggt ttcgacaaa atgatagtc 
        aaggcgcgc cctttgaaa aaactgtgc gagttcgag 
        ggggagccc ctgcctgat gactacaaa agcgactgt 
        taccaggac atggacgag acgaaatac gcatgcaag 
        gaaaaatac cgaattatg aaacggctc aaaaaggct 
        tcagaggaa taacacggc tttgattaa ctccctccc 
        cttcctttg cttaattaa cttaattta ttgtagtgg 
        tgggtgtta ggttttcct ctccctttt tcccgtttt 
        taatggacg ttctgaaga tgggtacgt aacaaagtg 
        taacaagag agggcgcgc gcgcttgct tgagggtgg 
        tgactgatt ttgtgacgt catctcact gtgattttt 
        gacagatgc ggatgcatc cgcctggca ctgacgagc 
        cgttcgatg gtaaaagac tcgcgagat ttatcgatg 
        ttgataagg aattttacg ttatgaaga ttgataaga 
        agacccatt tgaagaaca ggctggggt gacgtagct 
        ctagacact atactaatt ccctaattg ttgccttgt 
        atacggcca acttgattt aaacttttc cgcgataag 
        ttattgtgc tcagatggc gctcatttt tattttttt 
        tgtaaataa aataacgca tctaattac acaatattt 
        tttccgttg actgcacaa taatgtcaa tattaatca 
        attaccgtg gctcctggc ggcccattc aacggaaaa 
        caccaccat tacttacaa agatgcaaa aaatatgtc 
        ttacaaatt gcacttatc acttttgga tcacctaat 
        gattattgt caaatttca gtcgtgttt tttttgctt 
        ttaatttca attttgtcg cgtgattta aagagtaat 
        aatgttgta atccactca ttttttaat aggccattg 
        ataatctat attgtttaa agcacaaac gtgcgatag 
        ggagtgcct tgaaataag tattaaaca tttctgcat 
        gaaaatatt cttatttcc agttctgct taaaattaa 
        ttttctctt aggtttgat ttgacgtca ttaaatgat 
        tgtactaac gtgctcagg gttcaaaaa aagtatgga 
        attaagcat ttttctatt tactttacg aaaaaaata 
        atatttagt acttcat
      • >TC015025 RB
        gactttagc actacaata atgaattaa aaaggggct 
        aaaacttta gccccgacc aatttttta gggattttt 
        cgatttggg atgacagtt gttgggggg aaccagcgt 
        aaccacttg attcaaata aaaaaataa aaaaactca 
        ccaattttt cccaattaa aaccgcaat catccaagc 
        gtagcgtat taattgtgc gaccttctg ttgattaaa 
        tttgaatgt ttatgatct aacagtgaa aaatactta 
        caaggcaac aagtcgccc aattcacca attacgata 
        attatcact aaaaccgtc cagtcttca catgactca 
        ctgtaaacc cacctgtga cttcttctt acggtttac 
        ctgacgggc acccacttt gagtcataa actcgcagt 
        cataaaatt cggacttgt ggctaaatt ttactgttt 
        gatcgagtg gcccttgat agtaaaaaa acaacaagc 
        gattggtcg aacaaattg acacgtgac cataagttt 
        ttcaaaatt gcaatttta tgactggtt tatgacaaa 
        gctcgatgt tcgcttgtc ctttcacgc gtcgcggcc 
        aacgcgatt tcttcatct ggtaagttg agggccgct 
        ttgtgtctt tatcgagtg actaagtgc gaatctagt 
        cgtcggggt gcaaagaaa ttttgaaac aacgcaaaa 
        atgcaaacg tcactcttg atcctgtct ttggttgtc 
        ttcgtgggg gccaaagac atgatgcgg agcatagtc 
        ttcgacaag aacaccccc gacgtgttt tactgcccc 
        attcagaag cccacaggt ttcgacaaa atgatagtc 
        aaggcgcgc cctttgaaa aaactgtgc gagttcgag 
        ggggagccc ctgcctgat gactacaaa agcgactgt 
        taccaggac atggacgag acgaaatac gcatgcaag 
        gaaaaatac cgaattatg atgcggatg catccgcct 
        ggcactgac gagccgttc gatggtaaa agactcgcg 
        agatttatc gatgttgat aaggaattt tacgttatg 
        aagattgat aagaagacc catttgaag aacaggctg 
        gggtgacgt agctctaga cactatact aattcccta 
        attgttgcc ttgtatacg gccaacttg atttaaact 
        tttccgcga taagttatt gtgctcaga tggcgctca 
        tttttattt tttttgtaa ataaaataa cgcatctaa 
        ttacacaat attttttcc gttgactgc acaataatg 
        tcaatatta atcaattac cgtggctcc tggcggccc 
        attcaacgg aaaacacca ccattactt acaaagatg 
        caaaaaata tgtcttaca aattgcact tatcacttt 
        tggatcacc taatgatta ttgtcaaat ttcagtcgt 
        gtttttttt gcttttaat ttcaatttt gtcgcgtga 
        tttaaagag taataatgt tgtaatcca ctcattttt 
        taataggcc attgataat ctatattgt ttaaagcac 
        aaacgtgcg atagggagt gccttgaaa taagtatta 
        aacatttct gcatgaaaa tattcttat ttccagttc 
        tgcttaaaa ttaattttc tcttaggtt tgatttgac 
        gtcattaaa tgattgtac taacgtgct cagggttca 
        aaaaaagta tggaattaa gcatttttc tatttactt 
        tacgaaaaa aataatatt tagtacttc atcaagtgt 
        ataaaaatg taataataa tagttattt attata
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY