Status
Please wait ...
Gene information
General information
TC001382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15252271..15258435
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001382
    iBeetle screen iB_10571

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    head mostlynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen posterior segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001382 RA
      MSSNDNVPQ WKKELIARL RSQNKRTQQ LVVDSSSSH 
      QPTTSSVQQ PDGVGRERC GGEVTTKNM VQQERYWDE 
      LMSQKSDSD EDLHYGPGI VSKLKERYL SLAQRENKK 
      QRPTILRKA TSLENILDD AVAVNSNNE TAANRLFET 
      RLNGSNDTN KNANNRYRS TKRGGCEMK RARSVETIS 
      SSSSSTIEK ESKRESVHE EMLITINQE GNDKKLITT 
      PPRVNRPKR IPPLMNEKE KPPVDVVKQ AKMIFERRP 
      EQRTRPPQQ TGEVAAKVA TYKNIIRAA KKPAIKVKP 
      LVITNGVRV ANKPKECEK TKQVIVSPL EKTLPISPI 
      PDVSRIDPN EDATNKTSS LSETPDLII TSSPIPSVN 
      SPSYRISAT ENFLKEEIR LASISDNRK TSVKNHHLP 
      LQQNKNVSN TQTPPSQPL KLDINNVGE TKPNLTIVE 
      IEKNLINTA KTLEKSGGV IKNVEQVTI SKVPKVKKP 
      PREFENNSI VFKFTDRKD VPDYVQNDG VKRVAKLEK 
      PKVGEGGII LLPGASIGE SFTDEDEEI LRSLEGPPS 
      PCDVTFVND NILIDGKSS LSQKTKKAK MKISFVEEG 
      PEIYEYPSE SSLLIDDVP TSPPSAQMR HSVPSLAGT 
      SSLANYTPK STEEFQPGV TRSVQIVST KHENTDTEP 
      TLQQIEKPI LFSAGANSD ILF
    CDS sequence
    • >TC001382 RA
      atgtcctcg aacgataac gttccccaa tggaaaaag 
      gagttgata gcgcgtctc cgaagccaa aacaagcgc 
      acccagcag ctggttgtc gattcttcc tcctcccat 
      cagccgacg accagttcg gtgcaacaa cctgacgga 
      gttggacgt gagagatgt ggtggtgaa gtgacgacg 
      aaaaacatg gtgcaacaa gaaaggtat tgggacgaa 
      cttatgagc caaaagtct gattcggac gaagacttg 
      cactatggc ccaggtatt gtcagcaaa ctcaaagag 
      aggtacctt agtctcgct caaagagag aacaaaaaa 
      caaagaccc acaatttta cgtaaagcc acttcgttg 
      gagaatatt ttggatgat gcagtggct gtcaacagt 
      aataatgaa acagcagct aaccgtttg tttgaaacg 
      aggctgaac ggaagcaat gacaccaac aagaatgcg 
      aacaaccgg tataggagc acaaaacgt ggtggctgc 
      gaaatgaag agagctcgc tctgttgag acaatttca 
      tcatcatca tcatcaacg atcgaaaaa gaaagcaaa 
      agggaatca gtacatgag gaaatgctt atcacaatc 
      aaccaggaa ggaaatgat aagaaatta atcaccaca 
      ccacctaga gtcaatcga ccgaaaaga attcctcct 
      ctaatgaac gaaaaagaa aaaccacct gttgatgtg 
      gtcaagcaa gctaaaatg attttcgaa agaaggccc 
      gaacaaaga acaagacca cctcaacaa accggagaa 
      gtcgccgct aaagtagcc acttacaaa aacatcatc 
      agagctgca aaaaaacca gcaatcaaa gttaaacca 
      cttgttata accaatgga gttagggtc gctaataaa 
      ccaaaagaa tgcgaaaaa actaaacaa gtcattgta 
      tctccattg gaaaaaacc ttgcccatc agtccgata 
      ccggacgtt tcaagaatc gatccaaac gaagacgcc 
      acaaataaa acgtcaagt ctttcagaa acccccgat 
      cttataata acctctagt cccattcca tcagtaaat 
      tcaccatcg tatcggatc tcagcgact gagaatttc 
      ctcaaagaa gaaattcgt cttgcatca atcagcgat 
      aatagaaaa acgagcgta aaaaatcat catcttcca 
      ctccagcag aataaaaac gtgtcgaat actcaaaca 
      ccaccatct caaccgctc aaattggat atcaacaac 
      gtaggtgaa acgaaacca aatttgaca attgttgaa 
      attgaaaaa aacttaatc aacacggca aaaacatta 
      gaaaaaagt ggtggtgta attaaaaat gttgaacaa 
      gtgacgatt agtaaagtg ccaaaagtg aaaaaacca 
      ccacgcgaa ttcgagaat aactccatc gtttttaag 
      tttacggat aggaaagat gtcccggat tatgtacag 
      aatgatggt gttaaaagg gtggccaaa ttggagaaa 
      ccaaaggtg ggcgaaggt ggtattatt ttgcttcct 
      ggtgcctcg attggagaa tcgtttacc gacgaagat 
      gaggaaatt ttgagatct ttggaaggt ccaccaagt 
      ccctgtgat gtcacattt gttaacgat aacatactc 
      atcgatgga aaatctagt cttagtcaa aaaacgaaa 
      aaagccaaa atgaagata tcgttcgtg gaggaagga 
      ccggagatt tacgaatat ccgtcagag tcctctttg 
      ctcatagat gacgttccg acgtctcca ccaagtgca 
      caaatgcgt cattcagtt ccttctctc gcaggtaca 
      tcatcactg gcaaattac acgccgaaa agtaccgaa 
      gagtttcaa ccgggagta acacgaagt gttcaaatc 
      gtgtcgact aaacacgaa aataccgac accgaaccg 
      actttacaa caaatcgaa aaaccaatt ttatttagt 
      gccggggcc aattctgat atacttttc taa
    mRNA sequence
    • >TC001382 RA
      tgatacgaa tctataaca aatcccaac gaaatgaga 
      gtatttgca agaggcttt tttacgtct gttacataa 
      ttgtgagag gcatattaa tgtttgttg tgatacttg 
      tttatgaaa attgatgaa tggtaatac aggatccta 
      caagttgtt acaaattat ttggtagat gtcgcaaac 
      ttcgagcca tgcacagct tgtcacttt cattacgtc 
      atttgatat ttatcacag ttcttttta gcgttttta 
      ttcctacgt gcagtattg tataataga aagtagaat 
      taagtgtaa ggtgacagt gttagatct agtctagtt 
      gctctccac cgtaaacag ttcgcgttc tgtgataaa 
      tcgagtaaa ataccataa attgtgttc aagtgccgg 
      tataataac ttaaaaaaa ctcttaaaa acgttgaat 
      cgatcgatg aaaagccca tatgctgcc tgactgaca 
      ttacaatga tttacgatc gtcgtagat aagtgattg 
      tgataattt ggtgagaaa aattttaaa aatgtcctc 
      gaacgataa cgttcccca atggaaaaa ggagttgat 
      agcgcgtct ccgaagcca aaacaagcg cacccagca 
      gctggttgt cgattcttc ctcctccca tcagccgac 
      gaccagttc ggtgcaaca acctgacgg agttggacg 
      tgagagatg tggtggtga agtgacgac gaaaaacat 
      ggtgcaaca agaaaggta ttgggacga acttatgag 
      ccaaaagtc tgattcgga cgaagactt gcactatgg 
      cccaggtat tgtcagcaa actcaaaga gaggtacct 
      tagtctcgc tcaaagaga gaacaaaaa acaaagacc 
      cacaatttt acgtaaagc cacttcgtt ggagaatat 
      tttggatga tgcagtggc tgtcaacag taataatga 
      aacagcagc taaccgttt gtttgaaac gaggctgaa 
      cggaagcaa tgacaccaa caagaatgc gaacaaccg 
      gtataggag cacaaaacg tggtggctg cgaaatgaa 
      gagagctcg ctctgttga gacaatttc atcatcatc 
      atcatcaac gatcgaaaa agaaagcaa aagggaatc 
      agtacatga ggaaatgct tatcacaat caaccagga 
      aggaaatga taagaaatt aatcaccac accacctag 
      agtcaatcg accgaaaag aattcctcc tctaatgaa 
      cgaaaaaga aaaaccacc tgttgatgt ggtcaagca 
      agctaaaat gattttcga aagaaggcc cgaacaaag 
      aacaagacc acctcaaca aaccggaga agtcgccgc 
      taaagtagc cacttacaa aaacatcat cagagctgc 
      aaaaaaacc agcaatcaa agttaaacc acttgttat 
      aaccaatgg agttagggt cgctaataa accaaaaga 
      atgcgaaaa aactaaaca agtcattgt atctccatt 
      ggaaaaaac cttgcccat cagtccgat accggacgt 
      ttcaagaat cgatccaaa cgaagacgc cacaaataa 
      aacgtcaag tctttcaga aacccccga tcttataat 
      aacctctag tcccattcc atcagtaaa ttcaccatc 
      gtatcggat ctcagcgac tgagaattt cctcaaaga 
      agaaattcg tcttgcatc aatcagcga taatagaaa 
      aacgagcgt aaaaaatca tcatcttcc actccagca 
      gaataaaaa cgtgtcgaa tactcaaac accaccatc 
      tcaaccgct caaattgga tatcaacaa cgtaggtga 
      aacgaaacc aaatttgac aattgttga aattgaaaa 
      aaacttaat caacacggc aaaaacatt agaaaaaag 
      tggtggtgt aattaaaaa tgttgaaca agtgacgat 
      tagtaaagt gccaaaagt gaaaaaacc accacgcga 
      attcgagaa taactccat cgtttttaa gtttacgga 
      taggaaaga tgtcccgga ttatgtaca gaatgatgg 
      tgttaaaag ggtggccaa attggagaa accaaaggt 
      gggcgaagg tggtattat tttgcttcc tggtgcctc 
      gattggaga atcgtttac cgacgaaga tgaggaaat 
      tttgagatc tttggaagg tccaccaag tccctgtga 
      tgtcacatt tgttaacga taacatact catcgatgg 
      aaaatctag tcttagtca aaaaacgaa aaaagccaa 
      aatgaagat atcgttcgt ggaggaagg accggagat 
      ttacgaata tccgtcaga gtcctcttt gctcataga 
      tgacgttcc gacgtctcc accaagtgc acaaatgcg 
      tcattcagt tccttctct cgcaggtac atcatcact 
      ggcaaatta cacgccgaa aagtaccga agagtttca 
      accgggagt aacacgaag tgttcaaat cgtgtcgac 
      taaacacga aaataccga caccgaacc gactttaca 
      acaaatcga aaaaccaat tttatttag tgccggggc 
      caattctga tatactttt ctaattcca ttccaacgg 
      accataaat aatttgtaa tcgattttt acattttaa 
      acgttaatt ttagtttaa ctaaggtat gtattacta 
      ctgtggtaa gtttgattg aattcattc cactttaaa 
      tttgttcga aattctgtg attcaaaac agtgtgagt 
      ttttgtaaa ttttgttgt aactgcttt gtaaacatt 
      tatttaaat tgtatcgtc gagtgtatt actgttttc 
      ttttgcaaa ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY