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Gene information
General information
TC007113 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 9187586..9189059
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007113
    iBeetle screen iB_10570

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007113 RA
      MSHVELGFS EECEPWQVE SYQFPTKIG GKPAWLDFE 
      NLPKPENLQ CETCHEPLI FLCQIYAPY EHDERNFHR 
      TIFLFICRN PECCVKNSR NNVKAFRSS LPRRNKFYS 
      FEPPPDHSL DFSPSKWVS LCDLCGCLG EKKCGKCKK 
      ATYCSRDHQ VLDWKQGHK SDCEKGGTP RISSKLFPE 
      SIIVTEPEE IDEKSVDES EEVEKFNQL EREGKTGTM 
      SDVSDKELE KYVCDSDKA FIRFKKRIG DNNDQILRY 
      ERGGEPLWI ASDPKPDKV PNCEYCGNP RQYEFQIMP 
      QLFFVLHEN DLDVGIIIV YTCKESCVA GDNYKKEFV 
      FKQDVK
    CDS sequence
    • >TC007113 RA
      atgtcacac gtggaatta ggcttttcc gaggaatgt 
      gagccttgg caggtggaa agctatcaa tttcccacg 
      aaaataggc ggaaagccg gcttggctc gactttgaa 
      aatctgcca aaaccggaa aatttgcaa tgtgagacg 
      tgtcatgag cccctgatt ttcttgtgc caaatctac 
      gccccctac gaacacgac gagaggaat ttccatcgg 
      actattttc cttttcatc tgccgaaac cccgaatgt 
      tgtgtgaaa aactccagg aacaatgtg aaagcgttc 
      aggagctcg ttaccccga cgcaacaag ttttactcg 
      tttgagccg ccccccgac cacagtttg gatttttcc 
      ccgtcaaaa tgggtgtct ttgtgcgac ttgtgtggc 
      tgtttaggg gaaaaaaag tgtggcaag tgcaaaaaa 
      gcaacgtat tgtagtaga gaccatcag gtacttgat 
      tggaagcaa ggacacaag agtgactgc gaaaagggg 
      ggaacacca cgaatttca tcgaaattg ttccccgag 
      tcgataata gtcacagaa cctgaggaa attgatgaa 
      aaaagtgtc gatgaaagt gaggaagtt gaaaaattc 
      aatcagctt gaacgtgaa ggcaaaacg gggacaatg 
      agcgatgtg tcagataaa gaattggaa aaatatgtt 
      tgtgacagt gataaggca ttcattcgc ttcaaaaag 
      cgaatcggt gataacaac gatcagatt ctgcgatat 
      gaaagaggt ggagaacct ctgtggatt gccagcgat 
      cccaaacct gataaagtc ccgaattgc gaatattgc 
      gggaatccc agacaatac gagtttcaa atcatgccg 
      caattgttc tttgtgttg catgaaaat gacttggat 
      gtgggaatt attattgtt tatacttgt aaggagagt 
      tgtgtggcg ggtgataat tacaaaaag gaattcgtt 
      tttaaacag gacgttaag tag
    mRNA sequence
    • >TC007113 RA
      tcttttttt ggtaagttt gtggttgtt gtcagtcgg 
      ccataacct cacttttgc aatgtcaca cgtggaatt 
      aggcttttc cgaggaatg tgagccttg gcaggtgga 
      aagctatca atttcccac gaaaatagg cggaaagcc 
      ggcttggct cgactttga aaatctgcc aaaaccgga 
      aaatttgca atgtgagac gtgtcatga gcccctgat 
      tttcttgtg ccaaatcta cgcccccta cgaacacga 
      cgagaggaa tttccatcg gactatttt ccttttcat 
      ctgccgaaa ccccgaatg ttgtgtgaa aaactccag 
      gaacaatgt gaaagcgtt caggagctc gttaccccg 
      acgcaacaa gttttactc gtttgagcc gccccccga 
      ccacagttt ggatttttc cccgtcaaa atgggtgtc 
      tttgtgcga cttgtgtgg ctgtttagg ggaaaaaaa 
      gtgtggcaa gtgcaaaaa agcaacgta ttgtagtag 
      agaccatca ggtacttga ttggaagca aggacacaa 
      gagtgactg cgaaaaggg gggaacacc acgaatttc 
      atcgaaatt gttccccga gtcgataat agtcacaga 
      acctgagga aattgatga aaaaagtgt cgatgaaag 
      tgaggaagt tgaaaaatt caatcagct tgaacgtga 
      aggcaaaac ggggacaat gagcgatgt gtcagataa 
      agaattgga aaaatatgt ttgtgacag tgataaggc 
      attcattcg cttcaaaaa gcgaatcgg tgataacaa 
      cgatcagat tctgcgata tgaaagagg tggagaacc 
      tctgtggat tgccagcga tcccaaacc tgataaagt 
      cccgaattg cgaatattg cgggaatcc cagacaata 
      cgagtttca aatcatgcc gcaattgtt ctttgtgtt 
      gcatgaaaa tgacttgga tgtgggaat tattattgt 
      ttatacttg taaggagag ttgtgtggc gggtgataa 
      ttacaaaaa ggaattcgt ttttaaaca ggacgttaa 
      gtagctgat taaatcaat aaatatttt gtgcgtgtc 
      gcatcgttg gaaaaaaat cggattaga taagatttt 
      gtttgttga gtaagtttt agctagaag gggcttaat 
      cttgtaatg attacaaat gacaagtac aatttttcc 
      atacgtgtt aagttggca ccggcgaat tatttgatg 
      ctcagattg atcagaatc tattgtttt ataggaatt 
      gttcaataa atcaattat ttcgagttt ttggtttta 
      ttaagtaaa cacggtggt gagaaataa tttaattgc 
      aataattaa tgacacaag cgacctatc tcacaggaa 
      aatttcagg cgataataa ataataaaa aataaatac 
      tcaattggc acaaccctg acaaagctg tagttaagt 
      acggcaaga tttgtacta aaactattg aagtgac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY