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Gene information
General information
TC012068 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6769718..6771341
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012068
    iBeetle screen iB_10567

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax anterior partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012068 RA
      MASNNTSAV LDIVVESKE ISSILKDYD RSSICSGST 
      IDNSLVQSP SLESFSTIQ DIENLSELN MDENSDLSV 
      KIDNPQKHL ETLETYITF RITTKVARI EYSENEYVV 
      RRRYNDFIW LRQKLTECH PFCIVPPLP GKHSLIGQL 
      DRYSKDFIL LRMKALNVF VSRIVNHPI LSCNEHFKT 
      FLTAKQPDF NLHRRQRTN SENKIRTLS HSNSTHSAL 
      KNRHLEFDK TKSYLTVLS EKLSSIEKI SSRINKERI 
      DFIVELNSY HPIFTTWAT SEPELAALL QNIGCAVER 
      NTAAQNMLV QSYPTVIGN PIRDFLTYI DVVQDTIKK 
      REAYQCAYE NSLEELNKR HSEKDKLIA SSHNPSQAA 
      GFSLWKQPS CDEKLEKLG VYIPQLLKK VEANQDSLE 
      CANESLRSD IEHWQLEKQ QCLKKILLD FVNKQIEYY 
      QATVNAWEH VTSEIAPQN NVAVSK
    • >TC012068 RB
      MDENSDLSV KIDNPQKHL ETLETYITF RITTKVARI 
      EYSENEYVV RRRYNDFIW LRQKLTECH PFCIVPPLP 
      GKHSLIGQL DRYSKDFIL LRMKALNVF VSRIVNHPI 
      LSCNEHFKT FLTAKQPDF NLHRRQRTN SENKIRTLS 
      HSNSTHSAL KNRHLEFDK TKSYLTVLS EKLSSIEKI 
      SSRINKERI DFIVELNSY HPIFTTWAT SEPELAALL 
      QNIGCAVER NTAAQNMLV QSYPTVIGN PIRDFLTYI 
      DVVQDTIKK REAYQCAYE NSLEELNKR HSEKDKLIA 
      SSHNPSQAA GFSLWKQPS CDEKLEKLG VYIPQLLKK 
      VEANQDSLE CANESLRSD IEHWQLEKQ QCLKKILLD 
      FVNKQIEYY QATVNAWEH VTSEIAPQN NVAVSK
    CDS sequence
    • >TC012068 RA
      atggcaagt aataacacc tctgccgtt ttagacata 
      gtagtggag agtaaagaa atttcgtca attttaaaa 
      gattacgac cgctcaagc atatgtagc ggctcaacc 
      attgataat tctcttgtg caatcaccc agcctggaa 
      agtttttca acaatccaa gacatagaa aacttatcc 
      gagctaaac atggacgaa aactccgac ctgagtgtt 
      aaaatagac aatcctcag aagcacctc gagacgctt 
      gaaacctac atcaccttc agaataaca acaaaagtc 
      gcgcgtatc gaatactcg gagaacgaa tacgtcgtc 
      cgcagacgc tacaacgac ttcatttgg ttgcgtcag 
      aagctaacc gaatgccac cctttttgc atagtccct 
      ccactgccc ggcaagcac tcgctcata ggccaacta 
      gataggtac tcgaaagac ttcatccta ctgcggatg 
      aaagctttg aatgttttc gtttcgcgc atcgttaat 
      cacccgatt ttgagctgc aatgagcat ttcaagacg 
      ttcctcaca gccaaacaa cccgacttc aatttacac 
      cgccgacaa cgaaccaac tcagagaac aagatccgc 
      accttaagc cattccaac tcgactcac agcgcgctg 
      aaaaaccgc cacttggag ttcgacaaa accaaatcg 
      tacttaacc gtcttatcc gagaagctg tcctcaatc 
      gagaaaatt tctagtcgt attaataaa gagcgcatc 
      gattttatc gtagagctg aacagttac cacccgatt 
      ttcacaacg tgggccact agcgagccc gaactggcc 
      gcgctgttg cagaacatc gggtgcgct gtggagcgc 
      aataccgcc gctcagaac atgctagtc cagagttac 
      cccacggtt atcggtaac ccgatccgc gatttcctc 
      acttacatt gacgtggtc caagacacc ataaagaag 
      cgcgaggct tatcagtgc gcgtacgag aactcgttg 
      gaggagttg aacaagcgt cacagcgag aaagataag 
      ctgattgcg tcgtcacat aatccgtcc caagccgcc 
      ggcttttcg ctctggaag cagccgagc tgcgacgag 
      aagctggag aagttaggc gtttacatt ccgcagttg 
      ctgaagaag gtcgaggcg aaccaggac agtctggag 
      tgcgctaac gagtcgctc aggagcgac attgaacac 
      tggcagctc gaaaagcaa caatgtctt aaaaagatt 
      ttgcttgat tttgttaac aagcaaatt gagtactac 
      caagccact gtcaacgct tgggagcac gtcactagc 
      gaaattgcg ccacaaaac aacgtggcc gtgtccaaa 
      tga
    • >TC012068 RB
      atggacgaa aactccgac ctgagtgtt aaaatagac 
      aatcctcag aagcacctc gagacgctt gaaacctac 
      atcaccttc agaataaca acaaaagtc gcgcgtatc 
      gaatactcg gagaacgaa tacgtcgtc cgcagacgc 
      tacaacgac ttcatttgg ttgcgtcag aagctaacc 
      gaatgccac cctttttgc atagtccct ccactgccc 
      ggcaagcac tcgctcata ggccaacta gataggtac 
      tcgaaagac ttcatccta ctgcggatg aaagctttg 
      aatgttttc gtttcgcgc atcgttaat cacccgatt 
      ttgagctgc aatgagcat ttcaagacg ttcctcaca 
      gccaaacaa cccgacttc aatttacac cgccgacaa 
      cgaaccaac tcagagaac aagatccgc accttaagc 
      cattccaac tcgactcac agcgcgctg aaaaaccgc 
      cacttggag ttcgacaaa accaaatcg tacttaacc 
      gtcttatcc gagaagctg tcctcaatc gagaaaatt 
      tctagtcgt attaataaa gagcgcatc gattttatc 
      gtagagctg aacagttac cacccgatt ttcacaacg 
      tgggccact agcgagccc gaactggcc gcgctgttg 
      cagaacatc gggtgcgct gtggagcgc aataccgcc 
      gctcagaac atgctagtc cagagttac cccacggtt 
      atcggtaac ccgatccgc gatttcctc acttacatt 
      gacgtggtc caagacacc ataaagaag cgcgaggct 
      tatcagtgc gcgtacgag aactcgttg gaggagttg 
      aacaagcgt cacagcgag aaagataag ctgattgcg 
      tcgtcacat aatccgtcc caagccgcc ggcttttcg 
      ctctggaag cagccgagc tgcgacgag aagctggag 
      aagttaggc gtttacatt ccgcagttg ctgaagaag 
      gtcgaggcg aaccaggac agtctggag tgcgctaac 
      gagtcgctc aggagcgac attgaacac tggcagctc 
      gaaaagcaa caatgtctt aaaaagatt ttgcttgat 
      tttgttaac aagcaaatt gagtactac caagccact 
      gtcaacgct tgggagcac gtcactagc gaaattgcg 
      ccacaaaac aacgtggcc gtgtccaaa tga
    mRNA sequence
    • >TC012068 RA
      acctaacct cacttcatt acaaatact ttttattat 
      tatggcaag taataacac ctctgccgt tttagacat 
      agtagtgga gagtaaaga aatttcgtc aattttaaa 
      agattacga ccgctcaag catatgtag cggctcaac 
      cattgataa ttctcttgt gcaatcacc cagcctgga 
      aagtttttc aacaatcca agacataga aaacttatc 
      cgagctaaa catggacga aaactccga cctgagtgt 
      taaaataga caatcctca gaagcacct cgagacgct 
      tgaaaccta catcacctt cagaataac aacaaaagt 
      cgcgcgtat cgaatactc ggagaacga atacgtcgt 
      ccgcagacg ctacaacga cttcatttg gttgcgtca 
      gaagctaac cgaatgcca ccctttttg catagtccc 
      tccactgcc cggcaagca ctcgctcat aggccaact 
      agataggta ctcgaaaga cttcatcct actgcggat 
      gaaagcttt gaatgtttt cgtttcgcg catcgttaa 
      tcacccgat tttgagctg caatgagca tttcaagac 
      gttcctcac agccaaaca acccgactt caatttaca 
      ccgccgaca acgaaccaa ctcagagaa caagatccg 
      caccttaag ccattccaa ctcgactca cagcgcgct 
      gaaaaaccg ccacttgga gttcgacaa aaccaaatc 
      gtacttaac cgtcttatc cgagaagct gtcctcaat 
      cgagaaaat ttctagtcg tattaataa agagcgcat 
      cgattttat cgtagagct gaacagtta ccacccgat 
      tttcacaac gtgggccac tagcgagcc cgaactggc 
      cgcgctgtt gcagaacat cgggtgcgc tgtggagcg 
      caataccgc cgctcagaa catgctagt ccagagtta 
      ccccacggt tatcggtaa cccgatccg cgatttcct 
      cacttacat tgacgtggt ccaagacac cataaagaa 
      gcgcgaggc ttatcagtg cgcgtacga gaactcgtt 
      ggaggagtt gaacaagcg tcacagcga gaaagataa 
      gctgattgc gtcgtcaca taatccgtc ccaagccgc 
      cggcttttc gctctggaa gcagccgag ctgcgacga 
      gaagctgga gaagttagg cgtttacat tccgcagtt 
      gctgaagaa ggtcgaggc gaaccagga cagtctgga 
      gtgcgctaa cgagtcgct caggagcga cattgaaca 
      ctggcagct cgaaaagca acaatgtct taaaaagat 
      tttgcttga ttttgttaa caagcaaat tgagtacta 
      ccaagccac tgtcaacgc ttgggagca cgtcactag 
      cgaaattgc gccacaaaa caacgtggc cgtgtccaa 
      atgatataa aaagtactt gtagatctc actgtacat 
      tcctaactt atttaactt aatttaata tatttaata 
      aaatgct
    • >TC012068 RB
      acctaacct cacttcatt acaaatact ttttattat 
      tatggcaag taataacac ctctgccgt tttagacat 
      agtagtgga gagtaaaga aatttcgtc aattttaaa 
      agattacga ccgctcaag catatgtag cggctcaac 
      cattgataa ttctcttgt aggttgtgt tattggtgt 
      cacaaagtt aattaccag tgatttaag gtgcaatca 
      cccagcctg gaaagtttt tcaacaatc caagacata 
      gaaaactta tccgagcta aacatggac gaaaactcc 
      gacctgagt gttaaaata gacaatcct cagaagcac 
      ctcgagacg cttgaaacc tacatcacc ttcagaata 
      acaacaaaa gtcgcgcgt atcgaatac tcggagaac 
      gaatacgtc gtccgcaga cgctacaac gacttcatt 
      tggttgcgt cagaagcta accgaatgc caccctttt 
      tgcatagtc cctccactg cccggcaag cactcgctc 
      ataggccaa ctagatagg tactcgaaa gacttcatc 
      ctactgcgg atgaaagct ttgaatgtt ttcgtttcg 
      cgcatcgtt aatcacccg attttgagc tgcaatgag 
      catttcaag acgttcctc acagccaaa caacccgac 
      ttcaattta caccgccga caacgaacc aactcagag 
      aacaagatc cgcacctta agccattcc aactcgact 
      cacagcgcg ctgaaaaac cgccacttg gagttcgac 
      aaaaccaaa tcgtactta accgtctta tccgagaag 
      ctgtcctca atcgagaaa atttctagt cgtattaat 
      aaagagcgc atcgatttt atcgtagag ctgaacagt 
      taccacccg attttcaca acgtgggcc actagcgag 
      cccgaactg gccgcgctg ttgcagaac atcgggtgc 
      gctgtggag cgcaatacc gccgctcag aacatgcta 
      gtccagagt taccccacg gttatcggt aacccgatc 
      cgcgatttc ctcacttac attgacgtg gtccaagac 
      accataaag aagcgcgag gcttatcag tgcgcgtac 
      gagaactcg ttggaggag ttgaacaag cgtcacagc 
      gagaaagat aagctgatt gcgtcgtca cataatccg 
      tcccaagcc gccggcttt tcgctctgg aagcagccg 
      agctgcgac gagaagctg gagaagtta ggcgtttac 
      attccgcag ttgctgaag aaggtcgag gcgaaccag 
      gacagtctg gagtgcgct aacgagtcg ctcaggagc 
      gacattgaa cactggcag ctcgaaaag caacaatgt 
      cttaaaaag attttgctt gattttgtt aacaagcaa 
      attgagtac taccaagcc actgtcaac gcttgggag 
      cacgtcact agcgaaatt gcgccacaa aacaacgtg 
      gccgtgtcc aaatgatat aaaaagtac ttgtagatc 
      tcactgtac attcctaac ttatttaac ttaatttaa 
      tatatttaa taaaatgct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY