Status
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Gene information
General information
TC012407 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6701365..6706470
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012407
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10565

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva dorsal bent - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva cuticle dorsal irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva cuticle dorsal potentiallynot fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs potentially size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs podomer sometimes potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012407 RA
      MSAEGSVCE GEKPQPGPE ACVEDNSKQ SFRKKVWDY 
      LEKNKLVNF PRPAFGRIP NFKGADKAA AQLLQLEEF 
      KNAKSVEVN PDKPLEAAR SLVIESDKT LYVPVPRLQ 
      EGLLKKIAF CEADKPKNV KQTVTRWGI ETLGKAVDF 
      GDGLHIDLL VLGSVAVSR DGRRIGKGR GYADLEYAI 
      LKEMKAVDD NTTIVTTVH DSQVFDCLP SDIFKKYDV 
      PLDYILTPT QIIKVEKKL TRPEGIYWD ILSQRRLSL 
      MPVLQKIKE IHQSEGRDT TLKEEDTDV ESEDKPRKY 
      PKPRPKSNL AEQNELTDS GEKPRKRRF FRRTRSARI 
      TNENEDPGN KENNVTPPM KKKPRPFRR NPRRLQVDF 
      SLVVSNIDK NVRVRHLKD ALIDHGIKP NNITWRGYK 
      GFCYLHYAK QVKKEADKI VEEKPFAVD NVIEILQTL 
      KLNPESSNN LSVKVMEPI TRIETTDVT AV
    • >TC012407 RB
      MSAEGSVCE GEKPQPGPE ACVEDNSKQ SFRKKVWDY 
      LEKNKLVNF PRPAFGRIP NFKGADKAA AQLLQLEEF 
      KNAKSVEVN PDKPLEAAR SLVIESDKT LYVPVPRLQ 
      EGLLKKIAF CEADKPKNV KQTVTRWGI ETLGKAVDF 
      GDGLHIDLL VLGSVAVSR DGRRIGKGR GYADLEYAI 
      LKEMKAVDD NTTIVTTVH DSQVFDCLP SDIFKKYDV 
      PLDYILTPT QIIKVEKKL TRPEGIYWD ILSQRRLSL 
      MPVLQKIKE IHQRLVQKI ITRICLAVI GM
    • >TC012407 RC
      MKKKPRPFR RNPRRLQVD FSLVVSNID KNVRVRHLK 
      DALIDHGIK PNNITWRGY KGFCYLHYA KQVKKEADK 
      IVEEKPFAV DNVIEILQT LKLNPESSN NLSVKVMEP 
      ITRIETTDV TAV
    CDS sequence
    • >TC012407 RA
      atgagtgcc gaggggagc gtctgcgag ggcgagaag 
      ccccaaccg gggccggag gcatgcgtc gaggacaat 
      tcaaagcaa tcttttcgg aaaaaagtt tgggattat 
      ttagagaag aacaagttg gtgaacttt ccgcggccc 
      gcgttcggg cgcatcccc aacttcaag ggggctgac 
      aaggcggct gcccagctc ctgcagctc gaagagttc 
      aaaaacgcg aaatcggtc gaggtcaac ccggacaag 
      ccgctggag gcggcccgg agcctcgtc atcgagagc 
      gataagacc ctctacgtg cccgtgccc cggctgcag 
      gaggggctg ctgaagaaa atcgcgttc tgcgaggcc 
      gacaagccg aaaaacgtc aaacagacc gttacgcgc 
      tggggcatc gagaccctg ggcaaggcg gtggacttc 
      ggggacggc ctccacatt gacctgctg gtcctgggg 
      agcgtggcg gtgtccagg gacggccgc aggatcggc 
      aaggggcgc gggtacgcc gacctggag tacgcgatc 
      ctgaaggag atgaaggct gttgatgac aacaccacg 
      attgttacg acagtgcat gattcacaa gtgtttgat 
      tgtttgcca agtgacatt tttaaaaag tatgacgtg 
      cctcttgat tatatcctg actcccact caaattatc 
      aaagttgaa aagaaactg acaagaccc gagggaatt 
      tattgggat atcttgtca caaaggagg ctcagtctc 
      atgcccgtt ttacagaaa attaaggaa attcatcaa 
      agcgagggt cgagacacg actctgaag gaagaagac 
      accgacgtg gaaagcgag gataaacct cgaaagtac 
      ccaaaaccg cgaccaaag tcgaacctt gccgagcaa 
      aacgagctg acagattct ggcgaaaag ccgcgaaag 
      aggcggttt ttccggaga accaggtct gcgagaatc 
      acaaacgaa aacgaggac ccggggaac aaggaaaac 
      aacgttaca ccccctatg aagaagaag ccccggccc 
      ttccggcgc aacccccgc cgcctgcag gtcgacttc 
      tcgctcgtc gtgagcaac atcgacaaa aacgtccgc 
      gtgcgccac ctgaaagac gccctaatt gatcacggc 
      atcaagccg aacaacatc acatggcgc gggtacaaa 
      ggcttctgc tacttgcat tatgccaag caggtgaag 
      aaggaggcg gacaaaatt gttgaagaa aaaccgttc 
      gccgtcgat aacgttatc gaaatactc caaactttg 
      aaactcaac ccggaatct tcgaataat ttgagcgtc 
      aaagttatg gaaccaatt acgagaatt gaaacgact 
      gatgttaca gctgtttga
    • >TC012407 RB
      atgagtgcc gaggggagc gtctgcgag ggcgagaag 
      ccccaaccg gggccggag gcatgcgtc gaggacaat 
      tcaaagcaa tcttttcgg aaaaaagtt tgggattat 
      ttagagaag aacaagttg gtgaacttt ccgcggccc 
      gcgttcggg cgcatcccc aacttcaag ggggctgac 
      aaggcggct gcccagctc ctgcagctc gaagagttc 
      aaaaacgcg aaatcggtc gaggtcaac ccggacaag 
      ccgctggag gcggcccgg agcctcgtc atcgagagc 
      gataagacc ctctacgtg cccgtgccc cggctgcag 
      gaggggctg ctgaagaaa atcgcgttc tgcgaggcc 
      gacaagccg aaaaacgtc aaacagacc gttacgcgc 
      tggggcatc gagaccctg ggcaaggcg gtggacttc 
      ggggacggc ctccacatt gacctgctg gtcctgggg 
      agcgtggcg gtgtccagg gacggccgc aggatcggc 
      aaggggcgc gggtacgcc gacctggag tacgcgatc 
      ctgaaggag atgaaggct gttgatgac aacaccacg 
      attgttacg acagtgcat gattcacaa gtgtttgat 
      tgtttgcca agtgacatt tttaaaaag tatgacgtg 
      cctcttgat tatatcctg actcccact caaattatc 
      aaagttgaa aagaaactg acaagaccc gagggaatt 
      tattgggat atcttgtca caaaggagg ctcagtctc 
      atgcccgtt ttacagaaa attaaggaa attcatcaa 
      aggttagta caaaaaatt atcacgagg atatgtctt 
      gcagttatc ggaatgtga
    • >TC012407 RC
      atgaagaag aagccccgg cccttccgg cgcaacccc 
      cgccgcctg caggtcgac ttctcgctc gtcgtgagc 
      aacatcgac aaaaacgtc cgcgtgcgc cacctgaaa 
      gacgcccta attgatcac ggcatcaag ccgaacaac 
      atcacatgg cgcgggtac aaaggcttc tgctacttg 
      cattatgcc aagcaggtg aagaaggag gcggacaaa 
      attgttgaa gaaaaaccg ttcgccgtc gataacgtt 
      atcgaaata ctccaaact ttgaaactc aacccggaa 
      tcttcgaat aatttgagc gtcaaagtt atggaacca 
      attacgaga attgaaacg actgatgtt acagctgtt 
      tga
    mRNA sequence
    • >TC012407 RA
      gaaacgtca aaatgtgtc ggtaaccaa gaaaaagat 
      aaacacttc cgagtgtgt agttcttgc tttgctcgt 
      gcgtttttt tctaggatt tctgaatga aaagtgcgt 
      aatgagtgc cgaggggag cgtctgcga gggcgagaa 
      gccccaacc ggggccgga ggcatgcgt cgaggacaa 
      ttcaaagca atcttttcg gaaaaaagt ttgggatta 
      tttagagaa gaacaagtt ggtgaactt tccgcggcc 
      cgcgttcgg gcgcatccc caacttcaa gggggctga 
      caaggcggc tgcccagct cctgcagct cgaagagtt 
      caaaaacgc gaaatcggt cgaggtcaa cccggacaa 
      gccgctgga ggcggcccg gagcctcgt catcgagag 
      cgataagac cctctacgt gcccgtgcc ccggctgca 
      ggaggggct gctgaagaa aatcgcgtt ctgcgaggc 
      cgacaagcc gaaaaacgt caaacagac cgttacgcg 
      ctggggcat cgagaccct gggcaaggc ggtggactt 
      cggggacgg cctccacat tgacctgct ggtcctggg 
      gagcgtggc ggtgtccag ggacggccg caggatcgg 
      caaggggcg cgggtacgc cgacctgga gtacgcgat 
      cctgaagga gatgaaggc tgttgatga caacaccac 
      gattgttac gacagtgca tgattcaca agtgtttga 
      ttgtttgcc aagtgacat ttttaaaaa gtatgacgt 
      gcctcttga ttatatcct gactcccac tcaaattat 
      caaagttga aaagaaact gacaagacc cgagggaat 
      ttattggga tatcttgtc acaaaggag gctcagtct 
      catgcccgt tttacagaa aattaagga aattcatca 
      aagcgaggg tcgagacac gactctgaa ggaagaaga 
      caccgacgt ggaaagcga ggataaacc tcgaaagta 
      cccaaaacc gcgaccaaa gtcgaacct tgccgagca 
      aaacgagct gacagattc tggcgaaaa gccgcgaaa 
      gaggcggtt tttccggag aaccaggtc tgcgagaat 
      cacaaacga aaacgagga cccggggaa caaggaaaa 
      caacgttac accccctat gaagaagaa gccccggcc 
      cttccggcg caacccccg ccgcctgca ggtcgactt 
      ctcgctcgt cgtgagcaa catcgacaa aaacgtccg 
      cgtgcgcca cctgaaaga cgccctaat tgatcacgg 
      catcaagcc gaacaacat cacatggcg cgggtacaa 
      aggcttctg ctacttgca ttatgccaa gcaggtgaa 
      gaaggaggc ggacaaaat tgttgaaga aaaaccgtt 
      cgccgtcga taacgttat cgaaatact ccaaacttt 
      gaaactcaa cccggaatc ttcgaataa tttgagcgt 
      caaagttat ggaaccaat tacgagaat tgaaacgac 
      tgatgttac agctgtttg aggagaaaa caaatcttc 
      catctggct tccagtact tgccttagt agttatttt 
      tgagtttta ggtttgcct tgagtacaa gttttagtt 
      cctggattt gtaatctag aagaaaaga ctaattttt 
      ataacgttt gtgtatttt gttggactg aaagagaat 
      aaagttttt ttcaaaac
    • >TC012407 RB
      gaaacgtca aaatgtgtc ggtaaccaa gaaaaagat 
      aaacacttc cgagtgtgt agttcttgc tttgctcgt 
      gcgtttttt tctaggatt tctgaatga aaagtgcgt 
      aatgagtgc cgaggggag cgtctgcga gggcgagaa 
      gccccaacc ggggccgga ggcatgcgt cgaggacaa 
      ttcaaagca atcttttcg gaaaaaagt ttgggatta 
      tttagagaa gaacaagtt ggtgaactt tccgcggcc 
      cgcgttcgg gcgcatccc caacttcaa gggggctga 
      caaggcggc tgcccagct cctgcagct cgaagagtt 
      caaaaacgc gaaatcggt cgaggtcaa cccggacaa 
      gccgctgga ggcggcccg gagcctcgt catcgagag 
      cgataagac cctctacgt gcccgtgcc ccggctgca 
      ggaggggct gctgaagaa aatcgcgtt ctgcgaggc 
      cgacaagcc gaaaaacgt caaacagac cgttacgcg 
      ctggggcat cgagaccct gggcaaggc ggtggactt 
      cggggacgg cctccacat tgacctgct ggtcctggg 
      gagcgtggc ggtgtccag ggacggccg caggatcgg 
      caaggggcg cgggtacgc cgacctgga gtacgcgat 
      cctgaagga gatgaaggc tgttgatga caacaccac 
      gattgttac gacagtgca tgattcaca agtgtttga 
      ttgtttgcc aagtgacat ttttaaaaa gtatgacgt 
      gcctcttga ttatatcct gactcccac tcaaattat 
      caaagttga aaagaaact gacaagacc cgagggaat 
      ttattggga tatcttgtc acaaaggag gctcagtct 
      catgcccgt tttacagaa aattaagga aattcatca 
      aaggttagt acaaaaaat tatcacgag gatatgtct 
      tgcagttat cggaatgtg attgtagtt ttttgtgtt 
      ttttctatg tttgaaggc tgtagtttt atgccttca 
      gataattat tatttatta cccactaat tattatttt 
      tttggcaaa ttaggtgtt tatttactt tgaacatta 
      cactgtcca gtcttgttt ttacacaag agtgggtgt 
      attttttcg tgcttgaaa taacgtttt tttttgact 
      ctgagtatt tttattccc aatttcttg ttcagtcta 
      caaaaaaat gttcaatgg attaaagtc tggggcgtt 
      ttgggttag atcgcttcg atttataag aattttttc 
      gcttaaatt tattcagtt taactaaaa aaaaaaaaa 
      cagatttct ctctgattt tggtcgcca gaacctggg 
      ttcttctac gagtcgtaa caacttaaa atgcacgtc 
      aagctagtg aatatttat ttaaaaaaa acaagtatt 
      ttgtagaaa gataggttt tttgtaact tacaaccta 
      tttaaaaac tactctaaa gcacttgat atgtaacac 
      aaagttaaa ccaaaaact ggaataata tctgacatc 
      tcct
    • >TC012407 RC
      ccatagagc agtgatttc tcttcaagt gcctatgcg 
      gcaaatttt acaaatttt tactaagtg ttgtaacag 
      cctttgtgt cacaacttg tgatctttt tcctctaat 
      aactatcga aactcgcat ccatctact tgaataatc 
      tgtatctct gaagaattg tctattttt aattttttg 
      acaactcta gagtactcc agaagaatt attattttg 
      taggaattg ccacccttt gctattttt caataattt 
      taaacgtaa atgtcttgg cattccttc aaattgtaa 
      gtcaagtga tgttaaaac caaaaactt ggctgtcag 
      agactattt tggtgttaa atttattag tcaggtgga 
      taaattgca atatttgtt tattttttc tgataactg 
      atcgaaaat cagacaaaa aattacaaa aaacactta 
      gtaggattt agataatcc atgcaagtt atggattaa 
      gtgttgttg taatagaac caatttgat aattaaaaa 
      taaaacgag atttcgtcc aaacattta gttgttagg 
      gctcgaagt ttgtggtct cattgcatt tttttatat 
      aaaatctca aggtggtgt aagtatccc ctcattgta 
      actgcgaaa tagccaaaa aaagcgaca aaatttcaa 
      tataaattg aaagggtga aaaacgtta aaaccgtta 
      gcaacaggg ttaaaaatt cacaatttg tttgaaaaa 
      ttacgtata aagaggata attttaata attcacgaa 
      cttttaatt tattaatta tgcgaaaaa gctttgtat 
      gtcacatga gaccaaagt aacgttatt aatattgat 
      atgggtaag tgaaaatgc tctttcgtc tgccgtcac 
      gttttacgt tacatacaa gtttaaagg ggaaactag 
      tcgtaatgg cgcttttaa atccacact aattgtggt 
      cttgttttg acgttgcgt ttgctatgc aaacccgtt 
      tccaacccc tctgttcac ataacagta agtcttttg 
      ttgcgccac gttttcaca aaatgattt aagcgaggg 
      tcgagacac gactctgaa ggaagaaga caccgacgt 
      ggaaagcga ggataaacc tcgaaagta cccaaaacc 
      gcgaccaaa gtcgaacct tgccgagca aaacgagct 
      gacagattc tggcgaaaa gccgcgaaa gaggcggtt 
      tttccggag aaccaggtc tgcgagaat cacaaacga 
      aaacgagga cccggggaa caaggaaaa caacgttac 
      accccctat gaagaagaa gccccggcc cttccggcg 
      caacccccg ccgcctgca ggtcgactt ctcgctcgt 
      cgtgagcaa catcgacaa aaacgtccg cgtgcgcca 
      cctgaaaga cgccctaat tgatcacgg catcaagcc 
      gaacaacat cacatggcg cgggtacaa aggcttctg 
      ctacttgca ttatgccaa gcaggtgaa gaaggaggc 
      ggacaaaat tgttgaaga aaaaccgtt cgccgtcga 
      taacgttat cgaaatact ccaaacttt gaaactcaa 
      cccggaatc ttcgaataa tttgagcgt caaagttat 
      ggaaccaat tacgagaat tgaaacgac tgatgttac 
      agctgtttg aggagaaaa caaatcttc catctggct 
      tccagtact tgccttagt agttatttt tgagtttta 
      ggtttgcct tgagtacaa gttttagtt cctggattt 
      gtaatctag aagaaaaga ctaattttt ataacgttt 
      gtgtatttt gttggactg aaagagaat aaagttttt 
      ttcaaaac
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