Status
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Gene information
General information
TC006325 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7226061..7228483
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006325
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10563

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006325 RA
      MLSQNLEFW MSNLPQSLR QLPLIHLAI PGSHDSTTF 
      AITKKSKIS PDARNPIQY LKFLEPLLC PIMVKWSKT 
      QSVNVIQQL NAGIRYFDL RIATKKGCG GFYFVHNLY 
      SECVNGALA EIGQFLNTH KGEVVILDC QHFFNMGAA 
      DHCKFISLL VDTFGAKIQ PRTDLTQLT LLEMTERGK 
      QVIIIYRSD LREDFLWPG GSFPTPWAD TVFVPKLIS 
      FLNQTLKSR NYSLGFVSQ CVLTPTVWY VCKNIFSSL 
      VNKCVLPLE TAKYDWIRR QRAGPKGVN IVISDFIDY 
      QDYRFCKEV IDLNEKILN VY
    • >TC006325 RB
      MLSQNLEFW MSNLPQSLR QLPLIHLAI PGSHDSTTF 
      AITKKSKIS PDARNPIQY LKFLEPLLC PIMVKWSKT 
      QSVNVIQQL NAGIRYFDL RIATKKGCG GFYFVHNLY 
      SECVNGALA EIGQFLNTH KGEVRGITE LLQPDVTNF
    CDS sequence
    • >TC006325 RA
      atgctgtct caaaatttg gagttttgg atgtccaac 
      ttgccccaa agcctgcgc cagcttcca ctcattcat 
      ttggcaatt ccaggctcg cacgacagc acgactttt 
      gccataaca aaaaaatca aaaatatcg cccgatgcg 
      cgcaacccc atccagtat ttgaagttc ttggagccc 
      ctcttgtgc cccattatg gtcaaatgg tcgaaaacg 
      cagtctgtg aacgtcatt caacagtta aatgccggg 
      ataaggtat tttgatttg cggattgcg acaaaaaaa 
      gggtgtggg ggcttttat ttcgtccat aatttatac 
      tcagaatgc gtgaatggg gctttggcg gagattggt 
      caatttttg aatacacac aagggagag gttgtgata 
      ctagattgt cagcatttt tttaacatg ggggccgcc 
      gaccattgt aagttcatt tcgctactt gttgacacg 
      ttcggggct aaaatacag ccccggacc gatttaacg 
      caactcact ttactcgaa atgaccgaa cgcgggaaa 
      caagtcatt ataatttat cgatctgat ctcagagaa 
      gactttttg tggccgggg ggctctttc ccgactcct 
      tgggccgac acagtattt gtccccaaa cttatttcg 
      ttcctaaac cagactttg aaaagtcgg aattattcg 
      ctagggttt gtctcacag tgtgtttta acaccgacg 
      gtgtggtac gtttgcaaa aatattttt agttcgttg 
      gtaaacaaa tgcgtgttg ccgctagaa acggccaaa 
      tatgactgg ataaggcgc cagagggcg gggcctaag 
      ggtgtcaat atagtcatt tccgacttt atcgactat 
      caggattac aggttttgc aaagaagtg attgacttg 
      aacgaaaaa atacttaac gtttattag
    • >TC006325 RB
      atgctgtct caaaatttg gagttttgg atgtccaac 
      ttgccccaa agcctgcgc cagcttcca ctcattcat 
      ttggcaatt ccaggctcg cacgacagc acgactttt 
      gccataaca aaaaaatca aaaatatcg cccgatgcg 
      cgcaacccc atccagtat ttgaagttc ttggagccc 
      ctcttgtgc cccattatg gtcaaatgg tcgaaaacg 
      cagtctgtg aacgtcatt caacagtta aatgccggg 
      ataaggtat tttgatttg cggattgcg acaaaaaaa 
      gggtgtggg ggcttttat ttcgtccat aatttatac 
      tcagaatgc gtgaatggg gctttggcg gagattggt 
      caatttttg aatacacac aagggagag gttcgggga 
      attaccgaa ttgttacaa cctgacgtc accaatttc 
      tag
    mRNA sequence
    • >TC006325 RA
      tttaaatta tcaaataat ttatgcact gagttttac 
      cattttaag acaaaaaat tgtctgaaa ttgtatttt 
      ttttttcta aattagggc cgaaatcgt agttttttg 
      tcgatagaa ttaagctat tttaaattt gcgacaaca 
      ggtttagac attttgagt cagaataat ctaaaatca 
      aacaatttt aacaaactt ttcaggcag ccttaaagc 
      aatttctac ttttttgac tgcgagttt tgacccttt 
      ttccacaat atacaataa tgtaattta ccatgatgt 
      aaaaaacat gttattacc tttcttaaa aatttagtt 
      tcaaatttt ttgtttgtt cacatgctc ggtagagca 
      gttcgcctt taagcgcct tagtatatt cagccttaa 
      gaaacagtc tctaagtct tgccgatca ggtgtttac 
      tattttatg tgatatttt taatgcctt attgatgtg 
      ttgtgaaaa aactacttc gtaggtgtt gggactata 
      gttacctta aactgtgat ctatatact cagaaactt 
      cttataatt taacagata ataaactat tcatataca 
      ctacgacta caaaacaaa aatgaataa aaaaaatcc 
      aattccctt cctgttccc cgccaccct aacctcgaa 
      atgattaca aaaatccga taaaacccc gttatcgcg 
      cctaattat gtagtttgc gtccagtct acctcatta 
      taaattaat cactcggca aaatgctgt ctcaaaatt 
      tggagtttt ggatgtcca acttgcccc aaagcctgc 
      gccagcttc cactcattc atttggcaa ttccaggct 
      cgcacgaca gcacgactt ttgccataa caaaaaaat 
      caaaaatat cgcccgatg cgcgcaacc ccatccagt 
      atttgaagt tcttggagc ccctcttgt gccccatta 
      tggtcaaat ggtcgaaaa cgcagtctg tgaacgtca 
      ttcaacagt taaatgccg ggataaggt attttgatt 
      tgcggattg cgacaaaaa aagggtgtg ggggctttt 
      atttcgtcc ataatttat actcagaat gcgtgaatg 
      gggctttgg cggagattg gtcaatttt tgaatacac 
      acaagggag aggttgtga tactagatt gtcagcatt 
      tttttaaca tgggggccg ccgaccatt gtaagttca 
      tttcgctac ttgttgaca cgttcgggg ctaaaatac 
      agccccgga ccgatttaa cgcaactca ctttactcg 
      aaatgaccg aacgcggga aacaagtca ttataattt 
      atcgatctg atctcagag aagactttt tgtggccgg 
      ggggctctt tcccgactc cttgggccg acacagtat 
      ttgtcccca aacttattt cgttcctaa accagactt 
      tgaaaagtc ggaattatt cgctagggt ttgtctcac 
      agtgtgttt taacaccga cggtgtggt acgtttgca 
      aaaatattt ttagttcgt tggtaaaca aatgcgtgt 
      tgccgctag aaacggcca aatatgact ggataaggc 
      gccagaggg cggggccta agggtgtca atatagtca 
      tttccgact ttatcgact atcaggatt acaggtttt 
      gcaaagaag tgattgact tgaacgaaa aaatactta 
      acgtttatt aggattagg cgtaaagtt gtgattatg 
      acagtatta aacaagcag tttaacg
    • >TC006325 RB
      tttaaatta tcaaataat ttatgcact gagttttac 
      cattttaag acaaaaaat tgtctgaaa ttgtatttt 
      ttttttcta aattagggc cgaaatcgt agttttttg 
      tcgatagaa ttaagctat tttaaattt gcgacaaca 
      ggtttagac attttgagt cagaataat ctaaaatca 
      aacaatttt aacaaactt ttcaggcag ccttaaagc 
      aatttctac ttttttgac tgcgagttt tgacccttt 
      ttccacaat atacaataa tgtaattta ccatgatgt 
      aaaaaacat gttattacc tttcttaaa aatttagtt 
      tcaaatttt ttgtttgtt cacatgctc ggtagagca 
      gttcgcctt taagcgcct tagtatatt cagccttaa 
      gaaacagtc tctaagtct tgccgatca ggtgtttac 
      tattttatg tgatatttt taatgcctt attgatgtg 
      ttgtgaaaa aactacttc gtaggtgtt gggactata 
      gttacctta aactgtgat ctatatact cagaaactt 
      cttataatt taacagata ataaactat tcatataca 
      ctacgacta caaaacaaa aatgaataa aaaaaatcc 
      aattccctt cctgttccc cgccaccct aacctcgaa 
      atgattaca aaaatccga taaaacccc gttatcgcg 
      cctaattat gtagtttgc gtccagtct acctcatta 
      taaattaat cactcggca aaatgctgt ctcaaaatt 
      tggagtttt ggatgtcca acttgcccc aaagcctgc 
      gccagcttc cactcattc atttggcaa ttccaggct 
      cgcacgaca gcacgactt ttgccataa caaaaaaat 
      caaaaatat cgcccgatg cgcgcaacc ccatccagt 
      atttgaagt tcttggagc ccctcttgt gccccatta 
      tggtcaaat ggtcgaaaa cgcagtctg tgaacgtca 
      ttcaacagt taaatgccg ggataaggt attttgatt 
      tgcggattg cgacaaaaa aagggtgtg ggggctttt 
      atttcgtcc ataatttat actcagaat gcgtgaatg 
      gggctttgg cggagattg gtcaatttt tgaatacac 
      acaagggag aggttcggg gaattaccg aattgttac 
      aacctgacg tcaccaatt tctaggttg tgatactag 
      attgtcagc attttttta acatggggg ccgccgacc 
      attgtaagt tcatttcgc tacttgttg acacgttcg 
      gggctaaaa tacagcccc ggaccgatt taacgcaac 
      tcactttac tcgaaatga ccgaacgcg ggaaacaag 
      tcattataa tttatcgat ctgatctca gagaagact 
      ttttgtggc cggggggct ctttcccga ctccttggg 
      ccgacacag tatttgtcc ccaaactta tttcgttcc 
      taaaccaga ctttgaaaa gtcggaatt attcgctag 
      ggtttgtct cacagtgtg ttttaacac cgacggtgt 
      ggtacgttt gcaaaaata tttttagta agttgttat 
      agaaatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY