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Gene information
General information
TC000391 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12175970..12181287
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000391
    iBeetle screen iB_10562

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000391 RA
      MASSRPMSQ FAKLLKTGK VHDNQSVLY IPFNSHVAT 
      VKYERYNDL YDLIHTNIP DELQGQGLG TVFAERIFD 
      HLVENNKKI RLTCEFLHK FYIRNMDKY KGHVYYNLM 
      IAACKESAA KFKTESSYR LYQALALLL NNRLEESIN 
      EFDALKNEN TVKLSATIG LMYANKILG VSNKELFHK 
      LDSQVREYR KSAEAIDFC NSGFVLFVL NKPEKALDY 
      IDKSLNIQS DLVEALALK GWVLLHLKK LGHRVSQNI 
      TEIFEQALK DNKRNLDAI LGVSESYLT QNKFEEALD 
      AVNKAIVRY PKSVLPLIQ KMKIQFASQ DWEPTVETM 
      NRIINDKID NLDAQKINI LILLSRDAN YDEATVCIK 
      KYFHEIEIQ EPKNGKILS DSAKLFSRI CGRHVDVLK 
      ETHKMAERA IQINSEDVE FIVELGHQC LLSSRIKEA 
      QKYYKTASK LNESSLKAL MGLTLCELS ENGKSDQIK 
      KQVDFLLEL EDAQNSPQL YLIRAKICD NSDEALIFL 
      KKASDLQLS LVKHQYYSD AYLLSLDPD FMLDVVREF 
      LQYISYSSD FNSVRVTSS AASTALNAL KVVTKSCPG 
      LYEASFLLA KLQYLTGDT TNAMTTLEQ ILAKVEDES 
      SSEAHLLMA QIQVQNGYY ERAAQSLEV GLSYNFKVR 
      ENPMFHYIR GLVDKNLNN VPDSIKFLT TALTLVSLS 
      PQSTTYKSK DQSDLTLIE RASIYIELI EAHNTIGQN 
      DEAAKLLET AVEEFQGTP EEARILILS ADHAVKRKN 
      VQGAIDLLN KVKPNETYY LQARTKLAD ILLKHRLDS 
      YAYLQCYQD MVDENPGPE SYLLLGDAY MTILEPDEA 
      LECYNQALQ QNPKDPHLA LKMGQALVK THYFARAVA 
      FYKEAINTI QDPELRLQL GELYISMKE YDKAESLLS 
      SELKNDKSL DDLTQLQYK TKLSMLLAQ AREKSGNLP 
      GALTILREA RENQNRVRK RLAVEQTNV PEDEIKLLL 
      DIGVKLAEI AATLKDNDK AVQFYKDAL EISPSSTEI 
      LTALAKLYM QMNELDSCQ QICTNILSL DPENENASV 
      MMADIAFRK IDFDMALFH FTQLISKQP TNWTALVRL 
      VEVMRRTGN LTDFPEYLT NAEKKTQNP VKDPGFLFC 
      TALYQWYSG NLNGALRNF NASRQDATW GQQALYNMI 
      EICLNPDDE MLGDQFVDS EDIEYRDSR SMALKTAER 
      LLRELKHRL EAGGDDMLR YRLLVNFRL LATQEKYNI 
      EKGLEDFIS LGSQNMYKD NVGPVLGMV TAYTLLKQS 
      QRAKNQLKR VAKATWTFE DAEYLERCW LLLADYYIQ 
      SSKYDVAND LLTKVVQHN KTCSKAHEL LGFICEKEQ 
      HFKDAVVSY EAAWRFGGK TNVGIGYKL AYSLMKSKN 
      YADAIDVGQ QVLKLSPDY GKIKKDVLD KCLNNLRV
    CDS sequence
    • >TC000391 RA
      atggcttcc agtagacca atgtcacaa ttcgctaaa 
      ttattaaaa actggaaaa gtgcatgat aaccaaagc 
      gtcttatat atccctttt aattcacac gttgcaact 
      gttaaatat gaaagatat aacgacctt tacgattta 
      atccatacg aatattcct gatgagttg cagggtcaa 
      ggtttaggc actgttttt gcagagcga atttttgac 
      cacctggtt gaaaacaac aagaagata cgcttaacg 
      tgtgaattt ctgcataaa ttttatata cggaacatg 
      gataagtac aagggtcat gtttactac aatttaatg 
      atagcggcg tgtaaagag agcgctgca aagtttaag 
      actgaaagc tcctaccgt ttgtaccaa gcacttgcc 
      ttgctctta aataatcgt ttggaggaa agtataaac 
      gagttcgat gccttaaaa aatgaaaat actgtaaaa 
      ctctcagcc acaatcggg ttaatgtac gctaataaa 
      attctgggt gtctcgaac aaagaactg ttccataaa 
      ttagactcc caagttcga gagtatcga aagtcggcc 
      gaagcgatc gatttttgc aattccggc ttcgtcttg 
      tttgtttta aacaaaccg gagaaagcc cttgactac 
      atcgacaag tcgcttaat attcagtcg gacttggtc 
      gaagctttg gccctaaaa ggctgggtt ttgctccat 
      ttgaaaaaa ctgggccac agagtctca caaaacatt 
      acagaaata tttgaacaa gccttaaaa gacaacaaa 
      cggaacctg gacgccatt ttgggcgtc tctgaaagc 
      tacctaact cagaacaag tttgaggag gctcttgac 
      gcggtaaac aaggccata gttcgctac cccaagtca 
      gttctaccc ttaattcaa aaaatgaaa attcaattc 
      gcgtcccaa gactgggaa ccaaccgtt gagacaatg 
      aacaggatc attaacgac aaaatcgat aatctcgat 
      gcgcaaaaa atcaatatt ttgatttta ctgagccgt 
      gatgctaac tacgacgag gctacagtg tgcatcaaa 
      aaatatttt cacgaaatc gagatacaa gaacccaaa 
      aatgggaaa atcctctcc gatagcgcc aaactattc 
      tcgcgcatt tgcggccga catgttgac gtcctcaag 
      gagacgcac aaaatggcg gagcgagct attcaaata 
      aattcggag gatgtggag ttcattgtc gagttgggg 
      caccagtgt ttgctttca agccgcata aaagaggcc 
      caaaaatac tacaaaacg gcatcaaaa ttgaacgaa 
      tcctcgctg aaagccctg atgggcctc acactgtgc 
      gagctttcc gaaaacggc aaatctgac caaatcaaa 
      aagcaggtc gattttttg ctcgaactg gaagatgct 
      caaaactcg cctcaactg tacctaatc cgcgctaag 
      atttgcgac aactcggac gaggctctg atcttcctg 
      aagaaagca agtgatctc cagttgagt ctcgtcaaa 
      caccagtac tattctgac gcgtatttg ctgtcgctt 
      gatccagat ttcatgctg gacgttgtg agggaattc 
      ctccagtat atttcatac agtagtgac tttaactcg 
      gttcgggtc acttcaagt gccgccagc acggctcta 
      aacgcccta aaagtggtg actaagtcc tgtcccggc 
      ctttacgaa gcctcgttt ttgctggca aaacttcag 
      tacttgact ggggacacc actaacgcc atgaccact 
      cttgagcag attttggct aaagtggag gacgagtct 
      tcgagcgaa gctcacctc ttgatggcc caaatccaa 
      gtacagaac ggttattat gagagagct gcccaaagc 
      cttgaagtt gggttgagc tacaacttc aaggttcgg 
      gaaaatccg atgtttcat tacattcgg ggactggtt 
      gataaaaat ttaaataac gttcctgac tctattaaa 
      ttcctaact acggctcta actctggtc agtctcagt 
      ccacaaagt acgacatac aagagcaag gatcagagt 
      gacttgact ctgattgaa cgagcgtcc atttacata 
      gagctgatt gaagcacat aacacgatt ggccaaaac 
      gacgaagct gcaaaactt cttgagaca gcagttgaa 
      gagtttcaa gggacaccg gaagaggcc cgaattttg 
      attttaagc gcagaccac gccgttaaa aggaaaaac 
      gttcaaggg gccatagat ttattaaac aaagtcaag 
      ccgaacgaa acatactac ttgcaagct cggaccaaa 
      ctcgccgat attttactc aaacaccgc ctcgactcg 
      tacgcgtat ttgcaatgt taccaggac atggtggac 
      gaaaatccc ggccctgag agttacctt cttctgggc 
      gatgcttac atgacgatt ctagaacca gacgaagcc 
      ctggaatgc tataatcag gcccttcag caaaatccc 
      aaagacccc catcttgct ttaaagatg gggcaagcg 
      ctagtcaag acgcattat tttgcccgc gctgtggcc 
      ttctacaaa gaggcaatt aacacgatt caagacccg 
      gagttgagg cttcagctg ggcgagctt tacatctca 
      atgaaggag tacgacaag gctgaatct ttgctctcg 
      agtgagctt aaaaacgat aaatcgttg gacgacctg 
      actcagcta cagtacaaa actaagttg tcgatgttg 
      ttggcacaa gcccgcgaa aaatcgggg aatttacct 
      ggggcttta acaattctg agggaagcc agggagaat 
      cagaatcgg gtcaggaag cgcctggcg gtggaacaa 
      acaaacgtt ccggaagac gaaataaag cttctcctg 
      gacattggg gtgaaactg gccgagatc gccgcgact 
      ttgaaagac aacgataaa gcggtgcag ttttacaaa 
      gacgcgctg gaaatttcg cccagtagt accgaaatt 
      ttgacggct ttggccaaa ctatacatg cagatgaat 
      gaattagac tcgtgtcag caaatatgt accaacatc 
      ctgtctctg gacccagaa aatgaaaat gcgtcggtc 
      atgatggct gatatagcc ttcagaaag attgatttt 
      gacatggct ctgttccac ttcacccaa ctgatctcc 
      aaacaaccg accaactgg acggctctg gtgcgtcta 
      gtcgaagtg atgaggcgc acagggaac ttaaccgac 
      ttccccgaa tatttgaca aacgccgag aaaaagacg 
      caaaacccg gtgaaagac cccgggttt ttgttctgt 
      accgctctg taccagtgg tactccggt aacttgaat 
      ggggctttg cgcaacttc aatgcctcg cgtcaggat 
      gccacatgg gggcaacag gccttgtac aacatgatt 
      gaaatttgc ctgaaccct gacgatgag atgcttggc 
      gaccagttt gtcgattcg gaggatata gagtaccgg 
      gattctcgc agcatggct ttgaagacc gcggaacgg 
      ctgttacgc gagctgaag caccggctt gaagctggt 
      ggtgatgac atgctgcgg tataggctt ttggttaat 
      ttccgcctc ctggcgact caggagaag tacaacatt 
      gagaagggg cttgaggat tttatttcg ttgggttcg 
      cagaacatg tacaaggat aatgtgggg ccggttttg 
      ggtatggtg acggcttac accttgctg aagcagtcg 
      cagagagcg aagaatcag ctgaagagg gtggcgaaa 
      gccacttgg actttcgaa gacgcggag tatttggaa 
      cggtgttgg ttgttgctg gctgattac tacattcag 
      tcgtcgaag tacgacgtg gcgaatgat ttgttgacc 
      aaagtggtt cagcacaat aagacgtgc agtaaagcg 
      cacgagctg ctggggttc atttgcgag aaggagcag 
      cattttaag gacgcggtt gtgagctat gaagccgct 
      tggcgtttc ggcgggaag acgaacgtg gggatcggg 
      tacaagttg gcttacagc ctcatgaag agtaaaaat 
      tatgccgat gcgatcgat gtcgggcaa caggtgttg 
      aagttaagc cccgattat ggcaagatc aagaaggat 
      gtgctcgac aagtgcctg aataattta agggtttga
    mRNA sequence
    • >TC000391 RA
      tttgacaca tgtcatttt tgttgtgat tgaaaaacc 
      ggacaaaaa atcaatttt ttgcctttc tttcctgtg 
      tttttatta atttccttt ctcccatct ggcacatta 
      tggcttcca gtagaccaa tgtcacaat tcgctaaat 
      tattaaaaa ctggaaaag tgcatgata accaaagcg 
      tcttatata tccctttta attcacacg ttgcaactg 
      ttaaatatg aaagatata acgaccttt acgatttaa 
      tccatacga atattcctg atgagttgc agggtcaag 
      gtttaggca ctgtttttg cagagcgaa tttttgacc 
      acctggttg aaaacaaca agaagatac gcttaacgt 
      gtgaatttc tgcataaat tttatatac ggaacatgg 
      ataagtaca agggtcatg tttactaca atttaatga 
      tagcggcgt gtaaagaga gcgctgcaa agtttaaga 
      ctgaaagct cctaccgtt tgtaccaag cacttgcct 
      tgctcttaa ataatcgtt tggaggaaa gtataaacg 
      agttcgatg ccttaaaaa atgaaaata ctgtaaaac 
      tctcagcca caatcgggt taatgtacg ctaataaaa 
      ttctgggtg tctcgaaca aagaactgt tccataaat 
      tagactccc aagttcgag agtatcgaa agtcggccg 
      aagcgatcg atttttgca attccggct tcgtcttgt 
      ttgttttaa acaaaccgg agaaagccc ttgactaca 
      tcgacaagt cgcttaata ttcagtcgg acttggtcg 
      aagctttgg ccctaaaag gctgggttt tgctccatt 
      tgaaaaaac tgggccaca gagtctcac aaaacatta 
      cagaaatat ttgaacaag ccttaaaag acaacaaac 
      ggaacctgg acgccattt tgggcgtct ctgaaagct 
      acctaactc agaacaagt ttgaggagg ctcttgacg 
      cggtaaaca aggccatag ttcgctacc ccaagtcag 
      ttctaccct taattcaaa aaatgaaaa ttcaattcg 
      cgtcccaag actgggaac caaccgttg agacaatga 
      acaggatca ttaacgaca aaatcgata atctcgatg 
      cgcaaaaaa tcaatattt tgattttac tgagccgtg 
      atgctaact acgacgagg ctacagtgt gcatcaaaa 
      aatattttc acgaaatcg agatacaag aacccaaaa 
      atgggaaaa tcctctccg atagcgcca aactattct 
      cgcgcattt gcggccgac atgttgacg tcctcaagg 
      agacgcaca aaatggcgg agcgagcta ttcaaataa 
      attcggagg atgtggagt tcattgtcg agttggggc 
      accagtgtt tgctttcaa gccgcataa aagaggccc 
      aaaaatact acaaaacgg catcaaaat tgaacgaat 
      cctcgctga aagccctga tgggcctca cactgtgcg 
      agctttccg aaaacggca aatctgacc aaatcaaaa 
      agcaggtcg attttttgc tcgaactgg aagatgctc 
      aaaactcgc ctcaactgt acctaatcc gcgctaaga 
      tttgcgaca actcggacg aggctctga tcttcctga 
      agaaagcaa gtgatctcc agttgagtc tcgtcaaac 
      accagtact attctgacg cgtatttgc tgtcgcttg 
      atccagatt tcatgctgg acgttgtga gggaattcc 
      tccagtata tttcataca gtagtgact ttaactcgg 
      ttcgggtca cttcaagtg ccgccagca cggctctaa 
      acgccctaa aagtggtga ctaagtcct gtcccggcc 
      tttacgaag cctcgtttt tgctggcaa aacttcagt 
      acttgactg gggacacca ctaacgcca tgaccactc 
      ttgagcaga ttttggcta aagtggagg acgagtctt 
      cgagcgaag ctcacctct tgatggccc aaatccaag 
      tacagaacg gttattatg agagagctg cccaaagcc 
      ttgaagttg ggttgagct acaacttca aggttcggg 
      aaaatccga tgtttcatt acattcggg gactggttg 
      ataaaaatt taaataacg ttcctgact ctattaaat 
      tcctaacta cggctctaa ctctggtca gtctcagtc 
      cacaaagta cgacataca agagcaagg atcagagtg 
      acttgactc tgattgaac gagcgtcca tttacatag 
      agctgattg aagcacata acacgattg gccaaaacg 
      acgaagctg caaaacttc ttgagacag cagttgaag 
      agtttcaag ggacaccgg aagaggccc gaattttga 
      ttttaagcg cagaccacg ccgttaaaa ggaaaaacg 
      ttcaagggg ccatagatt tattaaaca aagtcaagc 
      cgaacgaaa catactact tgcaagctc ggaccaaac 
      tcgccgata ttttactca aacaccgcc tcgactcgt 
      acgcgtatt tgcaatgtt accaggaca tggtggacg 
      aaaatcccg gccctgaga gttaccttc ttctgggcg 
      atgcttaca tgacgattc tagaaccag acgaagccc 
      tggaatgct ataatcagg cccttcagc aaaatccca 
      aagaccccc atcttgctt taaagatgg ggcaagcgc 
      tagtcaaga cgcattatt ttgcccgcg ctgtggcct 
      tctacaaag aggcaatta acacgattc aagacccgg 
      agttgaggc ttcagctgg gcgagcttt acatctcaa 
      tgaaggagt acgacaagg ctgaatctt tgctctcga 
      gtgagctta aaaacgata aatcgttgg acgacctga 
      ctcagctac agtacaaaa ctaagttgt cgatgttgt 
      tggcacaag cccgcgaaa aatcgggga atttacctg 
      gggctttaa caattctga gggaagcca gggagaatc 
      agaatcggg tcaggaagc gcctggcgg tggaacaaa 
      caaacgttc cggaagacg aaataaagc ttctcctgg 
      acattgggg tgaaactgg ccgagatcg ccgcgactt 
      tgaaagaca acgataaag cggtgcagt tttacaaag 
      acgcgctgg aaatttcgc ccagtagta ccgaaattt 
      tgacggctt tggccaaac tatacatgc agatgaatg 
      aattagact cgtgtcagc aaatatgta ccaacatcc 
      tgtctctgg acccagaaa atgaaaatg cgtcggtca 
      tgatggctg atatagcct tcagaaaga ttgattttg 
      acatggctc tgttccact tcacccaac tgatctcca 
      aacaaccga ccaactgga cggctctgg tgcgtctag 
      tcgaagtga tgaggcgca cagggaact taaccgact 
      tccccgaat atttgacaa acgccgaga aaaagacgc 
      aaaacccgg tgaaagacc ccgggtttt tgttctgta 
      ccgctctgt accagtggt actccggta acttgaatg 
      gggctttgc gcaacttca atgcctcgc gtcaggatg 
      ccacatggg ggcaacagg ccttgtaca acatgattg 
      aaatttgcc tgaaccctg acgatgaga tgcttggcg 
      accagtttg tcgattcgg aggatatag agtaccggg 
      attctcgca gcatggctt tgaagaccg cggaacggc 
      tgttacgcg agctgaagc accggcttg aagctggtg 
      gtgatgaca tgctgcggt ataggcttt tggttaatt 
      tccgcctcc tggcgactc aggagaagt acaacattg 
      agaaggggc ttgaggatt ttatttcgt tgggttcgc 
      agaacatgt acaaggata atgtggggc cggttttgg 
      gtatggtga cggcttaca ccttgctga agcagtcgc 
      agagagcga agaatcagc tgaagaggg tggcgaaag 
      ccacttgga ctttcgaag acgcggagt atttggaac 
      ggtgttggt tgttgctgg ctgattact acattcagt 
      cgtcgaagt acgacgtgg cgaatgatt tgttgacca 
      aagtggttc agcacaata agacgtgca gtaaagcgc 
      acgagctgc tggggttca tttgcgaga aggagcagc 
      attttaagg acgcggttg tgagctatg aagccgctt 
      ggcgtttcg gcgggaaga cgaacgtgg ggatcgggt 
      acaagttgg cttacagcc tcatgaaga gtaaaaatt 
      atgccgatg cgatcgatg tcgggcaac aggtgttga 
      agttaagcc ccgattatg gcaagatca agaaggatg 
      tgctcgaca agtgcctga ataatttaa gggtttgaa 
      ggccggccg ataaaattt atagctttt atcaaagga 
      acttattgt tatttgcaa accgataaa tttaactac 
      cagttagta ataaatgtt ggcgcagca tatatagag 
      tagtgttag attgagccg tccactgcg aatttcggt 
      gtctcggca ttgtgattg atcaaatta ataaatagt 
      gtctcgatt cccgttggt ttgttttat ttttcgcga 
      catttattt ttacgccaa gcaataaag cttgtggca 
      aatggaaaa aaccagtgt tcattagat ggcagccaa 
      acacgtgca cgaagtatt tggaaagaa tctaatgat 
      gcaattatt tttgaattg aatttgttg gcaagttca 
      agtgttagt atcttagtg acagacaca agttttcta 
      tggagttat ggctaaaca tggtggcaa gggaaatcg 
      agtgatatg ggccggtag tgacattct cttcgatat 
      acagatctg tcccccgga tgacgacga cttcggaga 
      agatggtga gagtgagtt aaaaacgca t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY