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Gene information
General information
TC008772 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9715093..9729046
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008772
    iBeetle screen iB_10554

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008772 RA
      MATYAAYRH IELDKVGYS KKRVRNPSS 
      EIFNVLCTP PSNNNSPSS KLTPRRSSH 
      SDSFTRLFG TPEPKKVTP RRASLTKQD 
      VTNRNPVTG DGVASWDQK PGNRTTPKV 
      HNERNPVTG EVYTIVTPA TTPTKPVEN 
      GFAHVNGTS TPMLNGNHE PMNGNVATE
    CDS sequence
    • >TC008772 RA
      atggccact tatgctgct tacagacac atcgaactt 
      gacaaagtt ggctacagc aaaaagaga gtacgtaac 
      ccctccagc gagatcttt aatgtcctg tgcacgcca 
      ccgtcgaac aacaattcg ccatcctcg aagctaacg 
      ccgcgccga tcctcccat tccgactcc ttcacccgt 
      ttgtttgga accccggag cctaagaag gtgacacct 
      aggagagcc tcgttgaca aaacaagat gtcacaaat 
      cgcaaccca gtgactggt gacggagtc gcctcttgg 
      gaccaaaag ccagggaat cgtaccacg cccaaagtc 
      cataatgag agaaatcca gtaactgga gaggtgtac 
      accattgtg acccctgct acgacccca accaaacct 
      gtcgaaaac ggtttcgcc catgttaat ggtacttcc 
      actccgatg ctaaacgga aaccatgaa cccatgaat 
      gggaacgtc gcgactgaa taa
    mRNA sequence
    • >TC008772 RA
      ggaagtgca gttaaaatg gttaattcg aagtccgag 
      gttcatgga ctgaaagcg taaataaag cgacgtttc 
      ggaatttaa aaacgttga aacgtgcat tgaccagcg 
      gcaatctgg aggcgcgtt gacatttcg tacgtgtcg 
      gtcttattt cggccttca ttgtgatac attgactcc 
      ggaataatt atagggttg acgattctc atgacgtaa 
      ttgaatttt gctagagca aaagagaac gaggaagga 
      aagtgcgtt tgtgcagct gatgtgcgg atccacaca 
      tgacttgac aataataaa taaagtcgt aacaggatc 
      gccataacg gatgggttc tacttactt ttatttcat 
      acggaaaag ttgcttttt tcttatctt atttcatta 
      agataaggc ccgagtctg gaattccgc atgcttttt 
      ccataaacc tacatatat gaacatgaa aaacgtgac 
      gaatttgag ctatgattg atcgtaatg gaatcttaa 
      agcgaattg aagaccagg ggtcataaa tatcgggac 
      gaaaaacgg atttaccaa ttttacact ttctccggc 
      ttttccgcc tgaatagtt tcagaataa ttaaacaga 
      tacaaccag ccatttcta tgtttagat tacagcaat 
      tttctgcaa acaaggcga cacggtaca cggtactaa 
      ttatcggaa aaatgggcg caatctgag ccccagacc 
      gaaaccaaa tcagtgccc ctcctgcaa gggtggctt 
      gtaatccac cccttggaa ttaactgga aatccagaa 
      aatgcggtg aaacaataa tacaagtat ggatgaatg 
      cacatgatg gctataaaa gcaactact ttgcctcga 
      gctggcagt cagctgttg gcatttgca gcgagagct 
      tataaccgg agcgcgttt tttccgttt ttaccggca 
      tctacttgc gcttgttgt ttctttgcc agtgaccat 
      ggccactta tgctgctta cagacacat cgaacttga 
      caaagttgg ctacagcaa aaagagagt acgtaaccc 
      ctccagcga gatctttaa tgtcctgtg cacgccacc 
      gtcgaacaa caattcgcc atcctcgaa gctaacgcc 
      gcgccgatc ctcccattc cgactcctt cacccgttt 
      gtttggaac cccggagcc taagaaggt gacacctag 
      gagagcctc gttgacaaa acaagatgt cacaaatcg 
      caacccagt gactggtga cggagtcgc ctcttggga 
      ccaaaagcc agggaatcg taccacgcc caaagtcca 
      taatgagag aaatccagt aactggaga ggtgtacac 
      cattgtgac ccctgctac gaccccaac caaacctgt 
      cgaaaacgg tttcgccca tgttaatgg tacttccac 
      tccgatgct aaacggaaa ccatgaacc catgaatgg 
      gaacgtcgc gactgaata agtgctttt atccggact 
      tttcttttc tctacattc gtccatttt ttactgttc 
      tcctttgtt tcataaacc aaattttac tgtagaact 
      taaatatat acgtaggtt ttatatatt tttaagtaa 
      taaattata ctaacatct gggcactaa taaataaag 
      aagtattta tcgagtgcg actttccga gacggttaa 
      ctaatataa taaaatatt tattgcctc tcttaggta 
      tacattata agcaatata acacttcgg atttactag 
      tttatccat atcatcatg cctttatgc cttcataaa 
      tcatgttag tttgtaatt tggattaga agcttttat 
      atcatgttt ataattata ttacttcct aggagtagc 
      tttattttt acgttatgt acgaagagt attgtagaa 
      taaattttt atacacata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY