Status
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Gene information
General information
TC008913 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11750563..11752292
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008913
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
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      Email
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      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10553

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008913 RA
        MVDNTDNLE VGSTASSNI SESLRAQIL GRAHRRTRH 
        RPTTGNLQH RRALQNVVE ADESFEIVP QGPGQAESY 
        HTAEGDAGS RALVAASAD NVPKTPSAA SNRASAKRP 
        PSVTSDRVS DKRTPSLTS DRVSDKSSY TIQTQTVAI 
        QVGFDSDTE CCCGDYEYE DPHECCRCR RPEESSDCT 
        ETSYQTPAP KRRTRCTCR CRKNRRNYE SPESLSDIL 
        SDSNDSVCT EFMELPYQN NEEYYKLLK ELEKKLIAR 
        NKERVRRTM MEFEKRSRQ NQNLEKPVC HYEDSPKMG 
        RKKHKNSIC CKCGRNKRT NSDILQNEK QWEDLEYCH 
        AEPEPAPVS RTLKKCQWR IDPRTGEWV KVSFQQSDL 
        SAPCGDCDN TPVKKECCC RKKRH
      CDS sequence
      • >TC008913 RA
        atggtcgac aatacggac aatttggaa gtcgggagc 
        acagcgagc agcaacatt agcgagagt ctccgcgcc 
        caaattctg ggaagagcg caccgacgg acacggcac 
        cgccccacc accggcaac ctccagcac cggagggct 
        ctccagaac gtggtcgaa gccgacgaa tccttcgaa 
        atcgtcccc caaggcccc ggacaggcc gagagctac 
        cacacggcc gagggcgac gccggcagt cgcgccctg 
        gtggccgcc agcgccgac aacgtcccc aaaaccccc 
        tcggcagcc tcgaaccga gcttcggcc aaaaggccc 
        ccctcggta acctcggat cgagtctcc gacaaaaga 
        accccctcg ttgacttcg gaccgcgtc tcggacaag 
        agctcctac accatccag acgcagacc gtcgccatc 
        caagtcggc ttcgactcg gacaccgag tgctgctgc 
        ggggattac gagtacgag gacccgcac gagtgctgc 
        cgctgccgc aggcctgag gagagctcc gactgcacc 
        gaaacgtcg taccagacc cccgccccc aagcggcgc 
        actcgctgc acctgccgc tgccgcaaa aatcggagg 
        aactatgag tcaccggag agtctctcg gacattttg 
        agcgactca aacgactcc gtctgtacc gagtttatg 
        gaactgccg taccaaaac aacgaagag tattacaaa 
        ctgttgaag gagcttgag aaaaaactg atcgcgcgg 
        aataaggag cgcgtgcgg cgcacgatg atggagttt 
        gagaaacgc agtcgccag aaccaaaat ttggagaag 
        ccggtttgc cactacgag gacagcccg aaaatgggg 
        cggaaaaag cacaaaaat tcgatttgt tgcaagtgt 
        gggcggaac aagcggacg aattcggac attttgcaa 
        aatgagaag caatgggag gacttggag tattgtcac 
        gcggaacca gagccggcc ccggtttcg cgcactttg 
        aaaaaatgc cagtggcgg attgacccc cggactggg 
        gagtgggtc aaagtgtcg tttcagcag agcgatctt 
        tcggcgcct tgcggcgac tgcgacaac actcccgtc 
        aagaaagag tgttgttgt cggaaaaaa cggcactaa
      mRNA sequence
      • >TC008913 RA
        aattaaaaa ataatattt ttttcacct tcttgaagc 
        gaacacgat ctatcaaaa cgccccaca gtcttgtca 
        aaatctgac atcctgcaa tgcaaactc ccgtcccgc 
        aaaattcca agcagtgat ttgaaaaat ttcgtgaag 
        cgtttcttg cccaaattc accccaaat tctcccaat 
        ggtcgacaa tacggacaa tttggaagt cgggagcac 
        agcgagcag caacattag cgagagtct ccgcgccca 
        aattctggg aagagcgca ccgacggac acggcaccg 
        ccccaccac cggcaacct ccagcaccg gagggctct 
        ccagaacgt ggtcgaagc cgacgaatc cttcgaaat 
        cgtccccca aggccccgg acaggccga gagctacca 
        cacggccga gggcgacgc cggcagtcg cgccctggt 
        ggccgccag cgccgacaa cgtccccaa aaccccctc 
        ggcagcctc gaaccgagc ttcggccaa aaggccccc 
        ctcggtaac ctcggatcg agtctccga caaaagaac 
        cccctcgtt gacttcgga ccgcgtctc ggacaagag 
        ctcctacac catccagac gcagaccgt cgccatcca 
        agtcggctt cgactcgga caccgagtg ctgctgcgg 
        ggattacga gtacgagga cccgcacga gtgctgccg 
        ctgccgcag gcctgagga gagctccga ctgcaccga 
        aacgtcgta ccagacccc cgcccccaa gcggcgcac 
        tcgctgcac ctgccgctg ccgcaaaaa tcggaggaa 
        ctatgagtc accggagag tctctcgga cattttgag 
        cgactcaaa cgactccgt ctgtaccga gtttatgga 
        actgccgta ccaaaacaa cgaagagta ttacaaact 
        gttgaagga gcttgagaa aaaactgat cgcgcggaa 
        taaggagcg cgtgcggcg cacgatgat ggagtttga 
        gaaacgcag tcgccagaa ccaaaattt ggagaagcc 
        ggtttgcca ctacgagga cagcccgaa aatggggcg 
        gaaaaagca caaaaattc gatttgttg caagtgtgg 
        gcggaacaa gcggacgaa ttcggacat tttgcaaaa 
        tgagaagca atgggagga cttggagta ttgtcacgc 
        ggaaccaga gccggcccc ggtttcgcg cactttgaa 
        aaaatgcca gtggcggat tgacccccg gactgggga 
        gtgggtcaa agtgtcgtt tcagcagag cgatctttc 
        ggcgccttg cggcgactg cgacaacac tcccgtcaa 
        gaaagagtg ttgttgtcg gaaaaaacg gcactaaat 
        tttgtcccc taataagtg tcgggtaaa agtccgtgt 
        cagttagtc aggtcaagg gaaattaca atacaatgt 
        atttattaa c
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY